施肥量对泰兴香荷芋生长及产量效益的影响

通过对泰兴香荷芋施肥量研究,考查了各处理主要生育期的苗情与长势、成熟期子孙芋产量及商品芋产值效益。结果显示:基施腐熟脱水猪粪15t·hm-2,苗期追施(3.1叶)脱水猪粪15t·hm-2+13-10-18高钾复合肥675kg·hm-2的处理子孙芋产量最高为23 421.90kg·hm-2,商品芋效益为88 506.60元·hm-2;基施有机肥7 500kg·hm-2+苗期追施(3.1叶)有机肥7 500kg·hm-2+13-10-18高钾复合肥450kg·hm-2+膨大肥(8.1叶)13-10-18高钾复合肥900kg·hm-2的处理产量相对较高。     

不同供磷水平对胡麻磷素养分转运分配及其磷肥效率的影响简

 以“坝选３号”胡麻为材料，研究了不施磷、低磷、中磷和高磷４ 个不同施磷（Ｐ_２Ｏ_５）水平（０，３５，７０ 和１０５ｋｇ／ｈｍ^２）对胡麻植株中磷素累积、转运、分配和磷肥利用效率的影响。结果表明，低磷、中磷和高磷水平时，胡麻各器官不同生育阶段磷素养分吸收和累积量的基本趋势一致，但其变化量与施磷量有极大关系。胡麻地上茎、叶、非籽粒和籽粒中，磷素的日增量增幅因器官而异；胡麻只有叶片中有磷素转移，中磷处理比低磷和高磷处理磷转移量增加５４．９３％～７３．８３％和８．１９％～１０．００％（P＜０．０５），籽粒中２０．４６％～３５．９３％ 的磷素是靠叶片转运而来。胡麻植株磷素累积主要在生殖生长阶段，占整个生育期总累积量的７９．０２％～９２．１７％。施磷（Ｐ_２Ｏ_５）量为７０ｋｇ／ｈｍ^２时磷肥表观利用率和农学效率最高，分别为２０．２２％～２０．５３％和７．３０～７．４４ｋｇ／ｋｇ。胡麻产量随施磷量增加而增加，增幅最高达２８．９６％～３１．４６％。结合产量与磷肥表观利用率和农学效率，本实验区同等肥力土壤条件下，以施磷（Ｐ_２Ｏ_５）量为７０ｋｇ／ｈｍ^２（中磷）为宜。     

适用于转录组测序的大白菜小孢子总RNA提取方法筛选

为了找到适用于转录组测序要求的大白菜小孢子总RNA 提取方法，以出胚率较高的吉红82 为试材，以热激诱导处理后的小孢子为研究对象，比较分析了TRIzol 法、改良CTAB 法及超纯试剂盒法3 种方法的RNA 提取效果。结果表明：TRIzol法（磨样）和改良CTAB 法提取的RNA 浓度较高，但完整性较差；超纯RNA 提取试剂盒法提取的RNA 质量最高，且完整性好，能够满足转录组测序的要求。     

便携式XRF仪现场快速测定土壤中Pb的可行性研究

便携式XRF分析仪具有轻便、快速和可测对象较多与测量元素较广等特点而广泛应用于矿石、土壤等方面快速检测,但土壤的现场快速测定结果的准确性有待进一步研究与探讨。以安宁河谷平原土壤中Pb为研究对象,通过对便携式XRF仪现场快速检测结果与实验室分析结果的对比和影响因素的分析,建立基于安宁河谷平原表层土壤快速测定结果的线性函数为y=1.170 9x-17.326 0(R~2=0.992 9,x为现场检测结果),探讨其影响因素,为该区域表层土壤中Pb快速检测结果提供依据。     

Analysis of the independent-and interactive-photo-thermal effects on soybean flowering

Soybean(Glycine max(L.) Merr.) is a typical short-day and warm season plant, and the interval between emergence and flowering has long been known to be regulated by environmental factors, primarily photoperiod and temperature. While the effects of photoperiod and temperature on soybean flowering have been extensively studied, a dissection of the component photo-thermal effects has not been documented for Chinese germplasm. Our objective of the current study was to evaluate the independent- and interactive-photo-thermal responses of 71 cultivars from 6 ecotypes spanning the soybean production regions in China. These cultivars were subjected in pot experiments to different temperature regimes by planting in spring(low temperature(LT)) and summer(high temperature(HT)), and integrating with short day(SD, 12 h), natural day(ND, variable day-length), and long day(LD, 16 h) treatments over two years. The duration of the vegetative phase from emergence to first bloom(R1) was recorded, and the photo-thermal response was calculated. The outcome of this characterization led to the following conclusions:(1) There were significant differences in photo-thermal response among the different ecotypes. High-latitude ecotypes were less sensitive to the independent- and interactive-photo-thermal effects than low-latitude ecotypes; and(2) there was an interaction between photoperiod and temperature, with the effect of photoperiod on thermal sensitivity being greater under the LD than the SD condition, and with the effect of temperature on photoperiodic sensitivity being greater under the LT than the HT condition. The strengths and limitations of this study are discussed in terms of implications for current knowledge and future research directions. The study provides better understanding of photo-thermal effects on flowering in soybean genotypes from different ecotypes throughout China and of the implications for their adaptation more broadly.     

抚育间伐对燕山北部山区山杨林物种多样性的影响

为了了解抚育间伐对燕山北部山区山杨林物种多样性的影响,对山杨林抚育间伐前后的物种多样性进行了调查和分析,结果表明:抚育后林地草本植物的物种多样性高于未抚育林地,抚育间伐林地草本植物的Patrick指数、Margarlef指数、Simpson指数和Shannon-Wiener指数分别为35.0、6.13、0.89和2.76,未抚育样地分别为29.0、4.90、0.32和1.91;抚育林地灌木的Patrick指数、Margarlef指数、Simpson指数和Shannon-Wiener指数分别为9.0、1.41、0.43和0.98,未抚育林地则分别为8.0、1.48、0.68和1.51,除Patrick指数外,抚育林地的其他多样性指数都低于未抚育林地;抚育间伐使乔木层优势种的优势更为明显。由此得出结论,抚育间伐对山杨林的物种多样性有明显影响,但草本、灌木与乔木对抚育间伐的响应有所不同。     

Establishment and Optimization of INH Epitope Peptide Vaccine for Detection of Antibody

In this study, an indirect ELISA method was established to detect inhibin hormone (INH) epitope peptide vaccine antibody, it would provide oretical reference for the determination of Fine-wool sheep after active immune body INH epitope peptide vaccine antibody. On the basis of the predecessors, using indirect ELISA method to determinate serum INH epitope peptide antibody levels of sheep, and through control different experimental conditions to look for the best experimental conditions. Through explorating the experimental conditions, finally, the testing experiment conditions were determined, which was blocked solution with skimmed milk powder, INH and GnIH synthetic peptides dilution degrees for 20 000 times, the optimum reaction time was 60 min, the best color action time was 15 min. In this experiment, a kind of method to detection antibody in the body after INH active immune sheep was built, it would provide a reference for future research.     

Analysis on Target Genes with Mutation in microRNA of Blue-shelled Chicken and White Leghorn

The study was conducted to explore the potential different characters between Blue-shelled chicken and White leghorn.Global genome microRNA was combined the identified microRNA with complementary lab-predicted microRNA.Then the two breed chicken's SNP data got by GGRS were mapped to the microRNA and focused on SNP that deliberately located in mature-microRNA.Bioinformatics method was adopted for target prediction on microRNA which had SNPs.By further gene enrichment analysis,the study found these genes enriched in 22 GO terms,10 KEGG pathways,and 3 IPA important networks.And they enriched in traits which associated with growth,such as mTOR signaling pathways,Wnt signaling pathways,growth hormone receptor networks and insulin-like growth factor Ⅰ receptor networks.And they also enriched in some laying traits,such as oocyte meiotic signaling pathways and progesterone mature oocytes signaling pathways.The methods and the results might provide references for further studies.     

贵州农业发展现状及对策

定量化分析了新世纪以来贵州农业的发展,揭示了贵州农业新世纪以来在稳定粮食生产的基础上,在调整产业结构、经济作物生产上取得了长足的进展,2003-2012年贵州的蔬菜、水果产量分别增加了110.7%和84.4%。同时,分析了单产提高不快、化肥和农药用量增幅较快等当前存在的主要问题,并对未来贵州农业的发展提出了初步的对策。