The effect of glucocorticoid receptor on level of inflammatory media in rats after critical scald

AIM:To further elaborate the effect of glucocorticoid receptor(GR) decrease on level of inflammatory media in rats after critical scald.METHOD:The changes of phospholipase A₂(PLA₂) activity and concentrations of tumor necrosis factoralpha (TNFα) and malondialdehyde (MDA), the product of lipid peroxidation metabolism in plasma and tissue homogenate have been studied in scalded rats with or without GR blockade by RU38486.RESULTS:The PLA₂ activity and the concentrations of TNFα and MDA in plasma and homogenate of pulmonary and renal tissue in scalded rats were significantly higher than those in the controls(P＜0.01) and further increased in scalded rats accompanied with GR blockade (P＜0.01 or P＜0.05).CONCLUSION: Blockade of GR might aggravate the increase of inflammatory mediator caused by scalding. It suggested that GR might potentiate anti-inflammation in scald.     

Experimental research on cardiac fibrosis induced by recombinant mouse interleukin-11 in mice

AIM To observe the effect of recombinant mouse interleukin-11 （rmIL-11）injected subcutaneously into mice on heart structure and function and to determine its pro-fibrotic effect. METHODS C57BL/6 mice were randomly divided into experimental group and control group.The mice in experimental group were injected subcutaneously with recombinant mouse IL-11 at the dose of 100 μg·kg^-1·d^-1 for 3 consecutive weeks, while the control group were given equal volume of normal saline in the same way. After the experiment was finished, the parameters of heart function were measured by echocardiography.The heart weight was weighed and the cardiac weight index (CWI) was calculated. HE staining and Masson's trichrome staining were performed to observe the pathological changes and the extent of myocardial fibrosis in mouse myocardia respectively, and the cardiac collagen volume fraction (CVF) was calculated. The expression levels of extracellular matrix proteins in the myocardial tissues of mice, including type Ⅰ collagen, type Ⅲ collagen and fibronectin, were determined by Western blot. RESULTS Left ventricular ejection fraction and left ventricular fraction shortening in experimental group were obviously lower than those in control group (P<0.01), however left ventricular end-diastolic diamension and left ventricular end systolic dimension were significantly higher than those in control group (P<0.05).Compared with control group, the CWI was increased (P<0.01), the myocardial arrangement was disorder, the necrosis of cardiac myocytes was increased, and excessive deposition of collagen was observed in the myocardial tissues in experimental group. Correspondingly, the CVF and protein levels of type Ⅰ collagen, type Ⅲ collagen and fibronectin in the left ventricle in experimental group were increased significantly (P<0.05). CONCLUSION Injection of rmIL-11 into the mice subcutaneously induces fibrogenesis in the heart, which implies that IL-11 is likely a novel pro-fibrotic factor.     

伏毛铁棒锤种子萌发特性的研究

为探明伏毛铁棒锤(Aconitum flavumHand.-Mazz.)种子的萌发特性,采用了物理、化学、植物激素等不同处理方法对种子进行预处理.试验结果表明:①种子在常温(20±1)℃下有休眠现象,发芽率仅为1.3%;②低温15℃是种子发芽的最适宜温度;③机械去皮后种子发芽率有所提高,可达28%;④采用质量分数为100 mg/kg的赤霉素处理24 h,去皮种子发芽率最高可达到86.0%.因此,种皮障碍和内部萌发抑制物的存在是造成种子休眠的重要原因,生产上应首先去掉种子的外种皮,再用适宜浓度的赤霉素处理,会达到理想的出苗效果.     

农民工回流的原因及影响分析

农民工数量与日俱增,这从一个侧面折射了我国劳动力转移的成果。而与此同时发生的回流现象,也值得关注。文章从分析回流现象产生的原因与影响入手,结合实际,提出要系统地解决回流问题,就要认真对待其消极影响,注意回流人员的复杂性。     

燃烧室结构对天然气发动机燃烧过程的影响

为了研究燃烧室结构对天然气发动机燃烧过程的影响,模拟了浅盆形和涡流室式燃烧室结构的天然气发动机的进气、压缩及燃烧过程。给出了2种不同结构燃烧室的燃烧持续期、燃烧过程中缸内气流运动、火焰前锋面位置等,并对2种燃烧室进行了试验对比。模拟计算及试验结果表明,通过涡流室喷孔的约束作用,可以改善已燃气体和未燃气体之间的传热传质模式,提高火焰传播速度。     

一起东佛里生奶绵羊附红细胞体病流行病学调查、诊疗报告

为探究附红细胞体在内蒙古呼和浩特市托克托县地区的流行情况,接诊呼和浩特托县地区某东佛里生奶绵羊养殖场病例,该场近一周多圈舍出现产奶羊只体温升高、精神沉郁、瘫软、食欲减退或废绝等症状,有出现症状1 ~ 2 d迅速死亡病例,也有使用抗生素硫酸庆大霉素-青霉素联用结合补液治疗1周无好转或死亡病例,死亡总数超8只,仍有10余只相似症状正在接受治疗。经现场剖检、圈舍环境调查、样品实验室检测,结合临床诊疗,确诊此次疫情为奶绵羊附红细胞体病,且周边羊养殖场（户）羊只也存在附红细胞体感染。说明附红细胞体在托克托县呈地方性流行。     

核桃举肢蛾生物学特性的研究

核桃举肢蛾(Atrijuglans hitauhei Yang)是引起核桃早期落果的主要果实害虫之一,落果率达52%。该虫喜生活于阴坡地带,略具趋光性,飞翔、交尾、产卵均在傍晚。幼虫期共5龄,雌雄性比1∶1.14。果内危害30~40 d,发生情况受环境因子影响较大。     

来安县耕地质量等级特征研究

本研究结合来安县测土配方施肥土壤测试数据、新建高标准农田耕地质量等级数据、来安县第三次全国国土调查数据和来安县土壤图对来安县2022年耕地质量等级进行评价。利用县域耕地资源管理信息系统平台,建立来安县耕地质量评价数据库,完成2022年来安县耕地质量等级评价。结果显示2022年来安县耕地质量等级分布在1-9等,2022年耕地质量等级为4.375等,比2021年来安县耕地质量等级提升0.028等。2022年来安县耕地质量等级主要分布在3-6等,占来安县耕地面积的80.86%,其中5等地占23.10%,其次为3等地占比20.97%,6等地占比19.08%,4等地占比17.71%。     

浙江不同稻区耳叶水苋对苄嘧磺隆的抗性比较

采用琼脂法测定了长江三角洲地区宁绍平原和杭嘉湖平原5个稻区(宁波、绍兴、杭州、嘉兴和湖州)稻田耳叶水苋Ammannia arenaria不同生物型对苄嘧磺隆的抗性水平。结果表明,所采集的88种耳叶水苋生物型中,有96.6%已对苄嘧磺隆产生了抗性,其中,低抗(3RI≤10)、中抗(10RI≤50)和高抗(RI>50)的生物型分别占总数的22.7%、53.4%和20.5%。宁波NB0143-01和绍兴SX077生物型对苄嘧磺隆的RI值分别高达124.4和120.4。5个稻区中,绍兴地区耳叶水苋的抗性水平最高,平均RI值为53.1;宁波次之,平均RI值为35.1;湖州、杭州和嘉兴地区的抗性水平相对较低,平均RI值分别为24.1、19.9和18.7。表明稻田耳叶水苋对苄嘧磺隆的抗性程度较高,且在宁绍平原和杭嘉湖平原稻区已普遍出现抗性。其中在宁绍平原苄嘧磺隆对耳叶水苋的选择压较大,长期连续用药可能是该地区抗性水平高于其他区域的重要原因。这是有关耳叶水苋对苄嘧磺隆抗性种群分布的首次报道。