汽轮机本体常见故障检修

汽轮机发生故障通常会造成重大的经济损失和社会后果。通过汽封片原理与检修分析、积盐的分析和预防及处理轴找中心分析,指出了常见故障检修方法,旨在为同行提供借鉴。     

一起人布鲁氏菌病的畜间流行病学调查

2021年5月,湖南省长沙市发生一起人布鲁氏菌病疫情,确诊病例有共同暴露史,均接触或食用同一养殖场的羊胎衣。为确定疫情源头,防止疫情蔓延,消除疫情传播风险,通过现场走访询问、实验室检测等方式,对人确诊病例的共同暴露养殖场开展了畜间流行病学调查。调查结果显示:该养殖场饲养的山羊为外地调入且未经检疫,调入后出现疑似布鲁氏菌病症状并死亡;经实验室检测,存栏山羊的布鲁氏菌抗体阳性率为89.66%;相关人员普遍缺乏布病认知,防范意识不足,存在徒手接触羊流产物等情况,以及有食用羊胎衣的习俗。调查认为,养殖户外购未经检疫的羊是本次疫情的源头,相关人员布病防护意识低,对布病缺乏认知,导致感染。疫情确诊后,当地兽医主管部门按规定对存栏羊群进行全部扑杀和无害化处理,对栏舍进行全面彻底清洗消毒,依法查处违规调运等行为,使疫情得到有效控制。本次疫情警示,需要严格落实动物检疫和调运监管制度,做好监测预警,深化健康教育,提高群众自我防护意识,逐步净化消灭布病。     

Microbial remediation of a pentachloronitrobenzene-contaminated soil under Panax notoginseng: A field experiment

Pentachloronitrobenzene (PCNB) is an organochlorine fungicide that is mainly used in the prevention and control of diseases in crop seedlings. Microbial removal is used as a promising method for in-situ removal of many organic pesticides and pesticide residues. A short-term field experiment (1 year) was conducted to explore the potential role of a PCNB-degrading bacterial isolate, Cupriavidus sp. YNS-85, in the remediation of a PCNB-contaminated soil on which Panax notoginseng was grown. The following three treatments were used:i) control soil amended with wheat bran but without YNS-85, ii) soil with 0.15 kg m^-2 of solid bacterial inoculum (A), and iii) soil with 0.30 kg m^-2 of solid bacterial inoculum (B). The removal of soil PCNB during the microbial remediation was monitored using gas chromatography. Soil catalase and fluorescein diacetate (FDA) esterase activities were determined using spectrophotometry. In addition, cultivable bacteria, fungi, and actinomycetes were counted by plating serial dilutions, and the microbial biodiversity of the soil was analyzed using BIOLOG. After 1 year of in-situ remediation, the soil PCNB concentrations decreased significantly by 50.3% and 74.2% in treatments A and B, respectively, when compared with the uninoculated control. The soil catalase activity decreased in the presence of the bacterial isolate, the FDA esterase activity decreased in treatment A, but increased in treatment B. No significant changes in plant biomass, diversity of the soil microbial community, or physicochemical properties of the soil were observed between the control and inoculated groups (P<0.05). The results indicate that Cupriavidus sp. YNS-85 is a potential candidate for the remediation of PCNB-contaminated soils under P. notoginseng.     

植被恢复过程中侵蚀红壤有机质变化研究

基于2001年与2011年两期土壤样品数据,对江西省兴国县植被恢复过程中侵蚀红壤有机质的变化进行了研究。结果表明：10年间,纯林、林草及林灌草结构下表层(0 ~ 10 cm)土壤有机质变化值分别为?-5.8、-0.1及8.0 g/kg,千枚岩、红砂岩及花岗岩成土母质发育的红壤表层土壤有机质变化值分别为8.7、-0.5及 -3.1 g/kg。植被垂直结构对表层土壤有机质的变化影响显著,主要是由于植被覆盖度及生物量的不同引起的凋落物量的差异所导致;土壤颗粒组成是表层土壤有机质变化的重要影响因素之一,研究区表层土壤有机质的变化与黏粒含量的变化呈显著正相关(P < 0.05);此外,林下土壤侵蚀也影响着表层土壤有机质的变化。     

基于修正介电常数模型的煤矿区复垦土壤压实评价

模拟复垦机械对试验区土壤进行不同程度的压实,在探地雷达获取土壤介电常数的基础上,检验并修正了4种经典复合介电常数模型,并结合野外试验对修正后的模型通用性进行了验证。结果表明,土壤介电常数与土壤压实指标(土壤紧实度、容重、孔隙率等)相关系数89%,可以表征土壤压实状况;原有复合介电常数模型虽然存在误差不能直接使用,但模型计算值与实测值相关系数0.99,修正后二者拟合误差1%;在野外试验中,基于修正后的介电常数模型反算的土壤压实指标(容重、孔隙率)与实验室测量值误差率5%,通用性较好。表明在科学布设测线以保证探地雷达准确获取土壤介电常数的前提下,可以通过修正后的复合介电常数模型对煤矿区复垦土壤压实状况进行全面客观的评价。     

Growth kinetics of amyloid-like fibrils derived from individual subunits of soy β-conglycinin

The amyloid-like fibrillation of soy β-conglycinin subunits (α, α', and β) upon heating (0-20 h) at 85 °C and pH 2.0 was characterized using dynamic light scattering, circular dichroism (CD), binding to amyloid dyes (Thioflavin T and Congo red), and atomic force microscopy. The fibrillation of all three subunits was accompanied by progressive polypeptide hydrolysis. The hydrolysis behaviors, fibrillation kinetics, and morphologies of amyloid-like fibrils considerably varied among α, α', and β subunits. Faster hydrolysis rates and special fragments were observed for the α and α' subunits compared to the β subunit. However, the order of the fibrillation rate and capacity to form β-sheets was α' > β > α, as evidenced by CD and Thioflavin T data. Moreover, sequential growth of twisted screw-structure fibrils, leading to macroscopic fibrils with distinct morphological characteristics, was observed for β-conglycinin and individual subunits. The different fibrillation kinetics and morphologies of α, α', and β subunits appear to be associated with the differences in the amino acid composition and typical sequence of peptides. Besides, the disruption of ordered structure of fibrils occurred upon further heating (6-20 h) due to extensive hydrolysis. These results would suggest that all subunits are involved in the fibrillation of β-conglycinin, following multiple steps including polypeptide hydrolysis, assembly to amyloid structure, and growth into macroscopic fibrils with a fibril shaving process.     

Comparison of the chemical constituents of aged pu-erh tea, ripened pu-erh tea, and other teas using HPLC-DAD-ESI-MSn

Pu-erh tea is a popular beverage in southwestern China and South Asian countries. To explain the differences of aged pu-erh tea and ripened pu-erh tea, the chemical constituents of these teas were identified by HPLC-DAD-ESI-MS(n). In addition, HPLC was used to determine the contents of the major polyphenols, gallic acid, caffeine, and theobromine, in various types of teas. These results showed that the majority of chemical constituents in ripened pu-erh tea and aged pu-erh tea were similar, but the contents of catechins and gallic acid presented significant differences between these two teas. After fermentation by microorganism, the levels of catechins in ripened pu-erh tea were decreased, but the contents of gallic acid and caffeine were conversely elevated compared with aged pu-erh tea.     

生产建设项目用地土壤风蚀量监测方法与预报模型分析

准确掌握生产建设项目用地土壤风蚀量是合理编制水土流失治理方案的必要前提和有效防控人为水土流失的重要基础;但目前对监测方法和预报模型的研究比较薄弱,一些研究中监测方法的适用性和模型参数的准确性还有待商榷。文章系统总结土壤风蚀的观测方法和预报模型,根据这些方法和模型的各自特点,分析它们在生产建设项目中的适用性。认为测扦法、调查法、扫描法、风蚀盘法、集沙仪法、核素示踪法以及Pasak模型和风蚀流失量公式都可以根据不同项目的实际情况和地区特征应用到生产实践中。在此基础上,讨论相关研究的发展方向。本文结果可为生产建设项目土壤风蚀量测算模型的构建提供参考。     

群居型和散居型亚洲小车蝗成虫能量代谢相关酶活性测定

为明确亚洲小车蝗Oedaleus asiaticus成虫飞行肌对其体内能源物质利用的情况,采用室内生化法测定能源物质代谢相关5种酶,即3-磷酸甘油醛脱氢酶(glyceraldehyde phosphate dehydrogenase,GAPDH)、3-磷酸甘油脱氢酶(glycerol-3-phosphate dehyd...