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81.
Akiyoshi H Aoki M Shimada T Noda K Kumagai D Saleh N Sugii S Ohashi F 《American journal of veterinary research》2005,66(10):1830-1835
OBJECTIVE: To determine whether cross-reactivity exists between canine chromogranin A (CgA) and anti-human CgA antibody and investigate the usefulness of plasma CgA concentration measurements as an index of acute stress responses in dogs. ANIMALS: 12 healthy Beagles. PROCEDURE: Canine CgA was extracted and purified from canine adrenal glands of cadaver dogs for studying cross-reactivity with anti-human CgA antibody. Western blotting with anti-human CgA antibody was performed. Blood samples were collected from dogs at 0, 10, 20, 30, 40, 60, 120, and 180 minutes after IV administration of saline (0.9% NaCl) solution or insulin. Canine plasma CgA concentrations were determined by use of a CgA ELISA kit with rabbit antiserum against the carboxy-terminal fragment of human CgA. Plasma cortisol and catecholamine (ie, norepinephrine and epinephrine) concentrations were measured by use of an ELISA and a high-performance liquid chromatography method, respectively. RESULTS: Purified canine CgA was specifically detected by use of western blot analysis and an ELISA with anti-human CgA antibody. An increase in plasma CgA concentrations was observed in insulin-induced hypoglycemic dogs. Changes in plasma CgA concentration were correlated with changes in plasma cortisol or catecholamine concentrations of hypoglycemic dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Use of the CgA ELISA kit for determination of human plasma CgA concentrations is applicable to the measurement of canine plasma CgA concentrations. Canine plasma CgA concentrations, along with measurements of plasma cortisol and catecholamine concentrations, correctly reflect insulin-induced hypoglycemic stressed conditions in dogs. Measurement of canine plasma CgA concentrations may provide a useful index for evaluation of an acute stress response. 相似文献
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We previously developed insertion-deletion (InDel) markers that distinguish three genotypes (two homozygous and one heterozygous) of diverse citrus cultivars. These InDel markers were codominant and could be clearly detected by using simple agarose gel electrophoresis. We sought to establish a method for cultivar identification using these 28 InDel markers to genotype 31 citrus cultivars. The results revealed that a minimum of 6 markers were required to identify individuals using the three-genotype classification method. Furthermore, we found that a simple method for distinguishing between two genotypes (homozygous and heterozygous) could be used to identify individuals using a minimum of 7 markers. Our findings provide a basis for the development of simple and rapid citrus cultivar identification methods. 相似文献
85.
The characterization of autoallotetraploid hybrids between Iris ensata Thunb. and I. laevigata Fisch. 总被引:1,自引:0,他引:1
The characteristics of autoallotetraploid hybrids obtained from the cross between Iris ensata cv. Raspberry Rimmed (4X) and
amphidiploids of I. laevigata × I. ensata were examined and compared with those of their parents. The color of inner and outer
perianths in the autoallotetraploids were bluish purple and similar to those of the amphidiploid parent. However, the autoallotetraploids
exhibited low pollen fertility. In addition, the autoallotetraploids were characterized by 17 or 19 anthocyanins and had high
resemblance to their parents in the anthocyanin expression. Among these anthocyanins, malvidin 3RGac5G and petunidin 3RGac5G
were regarded as major anthocyanins in the autoallotetraploids and their parents, but the differences in the ratios of malvidin
3RGac5G:petunidin 3RGac5G between the autoallotetraploids and their parents were ca. 2:1 for the former and ca. 1:1 for the
latter. No viable hybrid seeds were obtained from the reciprocal crosses between I. ensata (2X and 4X) and the autoallotetraploids.
Finally, the interspecific cross-breeding of I. ensata using the autoallotetraploids is discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
86.
Choong-Min Ryu Beom Ryong Kang Song Hee Han Song Mi Cho Joseph W. Kloepper Anne J. Anderson Young Cheol Kim 《European journal of plant pathology / European Foundation for Plant Pathology》2007,119(4):383-390
The colonization of plant roots with certain rhizosphere bacteria promotes plant growth and induces long lasting systemic
protection against a broad spectrum of plant pathogens. The role of the global regulator, GacS, in the rhizosphere colonist
Pseudomonas chlororaphis O6 in stimulating growth promotion and induced resistance against Cucumber mosaic virus was examined in tobacco. Responses were compared in tobacco cvs Samsun and GX3. Root colonization of Samsun with wild-type
O6 and the gacS complemented mutant-elicited reduced viral symptoms and viral titre. On GX3, there was little affect on symptoms when roots
were colonized by the wild-type, gacS mutant or complemented mutant but colonization by both the wild-type and the gacS mutant lowered viral titre. Wild-type O6 and the gacS mutant caused plant growth to be maintained in both tobacco cultivars after viral infection, although the affect was stronger
with GX3 than Samsun. In contrast, although a chemical inducer, benzothiadiazole, reduced symptoms and viral titre in both
cultivars, plant growth was suppressed. Our results indicate rhizobacteria-elicited induced viral resistance without a negative
impact on growth but there was a differential response between cultivars. Detailed knowledge regarding the mechanisms inherent
to these differences between cultivars requires further investigation. 相似文献
87.
E. Ryu 《Canadian journal of veterinary research》1965,29(3):75-78
The nutritive value of 8 kinds of animal's sera for the growth of Leptospires was studied by a screening method and compared with that of rabbit serum.
The sera of dairy cattle and goats were higher than rabbit serum in average growth number, and thus gave great value for the cultivation of Leptospires.
In eight kinds of animals other than dairy cattle, the growth promoting action of the pooled sera for Leptospires were lower than that of the individual sera of the same kind of animals.
The serum which is to be added to the medium for the cultivation of Leptospires should be tested by a screening test for the absence of growth inhibitory action against Leptospires, no matter from what kind of animal the serum originated.
相似文献88.
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90.
Ali HA Sawada T Noda K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1603-1604
A 39 kDa protein of avian Pasteurella multocida strain P-1059 (serovar A:3) was purified from a crude capsular extract by immunoaffinity chromatography by using a ligand of purified mouse monoclonal antibody to the 39 kDa capsular protein of the strain. Protective activity of the purified 39 kDa protein antigen was determined by inoculation of ddY mice twice with 25 or 125 mug of the protein and challenge-exposure with 10 or 50 LD(50) of strains P-1059 or X-73 (serovar A:1). The results showed that the antigen gave high protection (60 to 100%). These results indicated that the 39 kDa protein of avian P. multocida is a cross-protective antigen over serovars A:1 and A:3. 相似文献