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961.
Inger Sundheim Fløistad Gro Hylen Kjersti Holt Hanssen Aksel Granhus 《New Forests》2018,49(2):231-247
Natural regeneration of Norway spruce (Picea abies (L.) Karst.) is a relatively common practice in Norway on medium to low site indices. However, seedling establishment is often hampered by rapid regrowth of competing vegetation in scarified patches. The aim of our study was to examine the effect of coordinating scarification towards an expected seed-fall, by studying germination and seedling establishment in scarified patches of different age (fresh, one- and two-year-old). The experiment was conducted in two stands in southeast Norway that were clear-cut in 2007. Scarification was applied to subplots in autumn 2008–2010. To simulate seed-fall, seeds were sown in fresh scarification patches in spring 2009–2011, in one-year-old patches in 2010 and 2011, and in two-year-old patches in 2011. Both germination and seedling survival were negatively affected by the age of the scarified patches. Germination was higher, and mortality lower, at the small fern woodland site, compared with the bilberry woodland site. Sowing in fresh patches also resulted in increased height and root collar diameter of the seedlings compared with sowing in older patches. It is likely that the competing vegetation both on the site and in the scarification patches affected the growth of the seedlings. In conclusion, the age of the scarified patches affected both germination and mortality, as well as early growth of the seedlings. 相似文献
962.
Domenico Rongai Claudio Cerato Luca Lazzeri 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(4):613-619
This study examines the effects of a vegetable fungicide on sugar beet powdery mildew (Erysiphe betae) and cucumber powdery mildew (Erysiphe cichoracearum). The formulations consisting of a dispersion of Brassicaceae meal in vegetable or mineral oils on infected leaves of sugar beet, reared in the greenhouse, and of musk melons cultivated
under plastic tunnels, were tested in comparison to each oil taken separately. Both formulations containing Brassicaceae meals, caused 94% of conidia to be distorted while for the untreated group only 2% were distorted. Furthermore, the leaf
area infected by E. betae was 56% for untreated plants and 2.7 and 9.9% respectively, for plants treated with meal containing mineral and vegetable
oil. Vegetable oil considered separately or with Brassicaceae meals showed no phytotoxicity, while the formulations based on mineral oil showed a significantly lower fresh and dry weight
on tomato plants. The low level or absence of phytotoxicity of plants treated with vegetable oil formulations suggests that
to improve the efficacy of powdery mildew control, they could be used mixed with sulphur. The efficiency of the vegetable
formulations in the powdery mildew control observed during these trials encourages further investigation on other parasitic
fungi and foliar pathogens. 相似文献
963.
An experiment was conducted to investigate the effects of earthworm (Metaphire guillelmi) activities on rice photosynthates distribution in plant–soil system through 14C pulse-labelling method. Rice was planted in pots, and maize straw was mulched on the surface with or without earthworms.
Rice plants at tillering stage or heading stage were labelled with 14CO2. Plant and soil were sampled 15 days after labelling at the tillering or heading stage and at harvest. Rice growth was inhibited
by earthworms (M. guillelmi) at early stage, but the inhibition disappeared at later stage. Earthworms significantly (P < 0.01) increased the 14C percentage in root at day 15 after tillering stage labelling, but the effect disappeared at harvest. Earthworms (M. guillelmi) significantly (P < 0.01) increased the 14C percentage in root at day 15 after heading stage labelling and increased 14C percentage in soil at harvest. Earthworms decreased the percentages of total organic 14C (TO14C) present as microbial biomass 14C (MB14C) and increased the percentages of total organic 14C present as dissolved organic 14C (DO14C) at all sampling times. It is suggested that earthworms might alter the transfer of plant photosynthates from the aboveground
to the belowground, and thus, soil active C pool. However, these data should be also confirmed in the field. 相似文献
964.
Daniella Fatmata Sesay Habte-Michael Habte-Tsion Qunlan Zhou Mingchun Ren Jun Xie Bo Liu Ruli Chen Liangkun Pan 《Fish physiology and biochemistry》2017,43(4):923-940
The effects of dietary folic acid on biochemical parameters and gene expression of three heat shock proteins (HSPs) of blunt snout bream (Megalobrama amblycephala) fingerling under acute high temperature stress. Six dietary folic acid groups (0.0, 0.5, 1.0, 2.0, 5.0, and 10.0) mg/kg diets were designed and assigned into 18 tanks in three replicates each (300 l/tank) and were administered for 10 weeks in a re-circulated water system. The fingerlings with an initial weight of 27.0 ± 0.03 g were fed with their respective diets four times daily. At the end of the experiment, samples were collected before challenge, 0, 24, 72 h, and 7 days. Serum total protein (TP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), cortisol, glucose, complement C3 (C3), complement C4 (C4, immunoglobulin M (IgM) hepatic superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and the expression of heat shock protein 60 (HSP60), 70 (HSP70), and 90 (HSP90) were studied. The results showed that fish fed with dietary folic acid between 1.0, 2.0, and 5.0 mg/kg significantly (P < 0.05) increased serum TP, C3, C4 hepatic SOD, CAT, and the expression of HSP60, HSP70, and HSP90 before and after temperature challenge of 32 °C. Also, serum ALP, cortisol, glucose, and hepatic MDA were significantly (P < 0.05) reduced by supplementation of dietary folic acid level 1.0, 2.0, and 5.0 mg/kg before and after the same temperature challenge of 32 °C. Before stress, 0, 24, 72 h, and 7 days significantly (P < 0.05) affects serum biochemical parameters, immune and antioxidant capacities, and expression level of three HSPs. Furthermore, there was no statistical evidence to show that dietary folic acid inclusion level and temperature duration have significant interactive effect on serum biochemical parameters, antioxidant parameters, and gene expression level (P > 0.05) of the three HSPs. However, there were statistical significant interactive effect between dietary folic acid inclusion level and temperature duration on serum C3 and C4 (P < 0.05) except IgM (P > 0.05). The present results indicate that supplementation of basal diet from 1.0 mg/kg; 2.0 and 5.0 mg/kg can enhance acute high temperature resistance ability in M. amblycephala fingerling to some degree and improve physiological response, immune and antioxidant capacities, and expression level of three HSPs. 相似文献
965.
Eduardo Luis Cupertino Ballester Fabrício Martins Dutra Tito Luís Pisseti Ronaldo Olivera Cavalli Paulo César Abreu Wilson WasieleskyJr 《Aquaculture International》2018,26(3):713-726
The objective of the present work was to determine the influence of artificial substrates that increase the area for biofilm development on the production performance of Farfantepenaeus paulensis juveniles in pens. Shrimp were stocked at a density of 20/m2 in pen structures (bottom area?=?50 m2) that were installed in the Patos Lagoon estuary. Two treatments with three repetitions were analyzed, where artificial substrates (polyethylene nets—1-mm mesh size) were added to increase the area for biofilm development by 100%, and where no substrates were added. During the experimental period, the biomass and the composition of the biofilm were assessed. After 86 days of rearing, no significant differences were found in shrimp performance between the treatments (p?>?0.05). However, the examination of the chlorophyll a, dry weight, and composition of the biofilm indicated that the shrimp were actively consuming the biofilm attached to the artificial substrates. Significant decreases in the abundances of nematodes >?500 μm after the 56th day and of tintinnids and rotifers between day 28 and day 42, indicated that the shrimp were selectively predating on these organisms. Moreover, a decrease in the chlorophyll a concentration in the biofilm suggests that the shrimp were consuming the microalgae. Although the increase in the area for biofilm development did not improve shrimp performance, the shrimp presented the highest growth rates when they consumed most of the biofilm microorganisms. 相似文献
966.
Morrow EM Yoo SY Flavell SW Kim TK Lin Y Hill RS Mukaddes NM Balkhy S Gascon G Hashmi A Al-Saad S Ware J Joseph RM Greenblatt R Gleason D Ertelt JA Apse KA Bodell A Partlow JN Barry B Yao H Markianos K Ferland RJ Greenberg ME Walsh CA 《Science (New York, N.Y.)》2008,321(5886):218-223
To find inherited causes of autism-spectrum disorders, we studied families in which parents share ancestors, enhancing the role of inherited factors. We mapped several loci, some containing large, inherited, homozygous deletions that are likely mutations. The largest deletions implicated genes, including PCDH10 (protocadherin 10) and DIA1 (deleted in autism1, or c3orf58), whose level of expression changes in response to neuronal activity, a marker of genes involved in synaptic changes that underlie learning. A subset of genes, including NHE9 (Na+/H+ exchanger 9), showed additional potential mutations in patients with unrelated parents. Our findings highlight the utility of "homozygosity mapping" in heterogeneous disorders like autism but also suggest that defective regulation of gene expression after neural activity may be a mechanism common to seemingly diverse autism mutations. 相似文献
967.
Parker JE Timm KI Smith BB Van Saun RJ Winters KM Sukon P Snow CM 《American journal of veterinary research》2002,63(7):948-953
OBJECTIVE: To evaluate temporal changes in bone mineral density associated with seasonal variation in serum vitamin D, calcium, and phosphorus concentrations in alpacas. ANIMALS: 5 healthy mature neutered male alpacas. PROCEDURE: Metacarpal bone mineral density was measured at 4 times during a year. Each time alpacas were weighed, blood was collected for determination of serum calcium, phosphorus, and vitamin D concentrations, and samples of feed were analyzed for nutrient content. Vitamin D status was determined by use of an assay that measured serum 25-hydroxycalciferol concentration. Effects of changes in serum vitamin D, calcium, and phosphorus concentration and body weight with season on bone mineral density were determined. RESULTS: Bone mineral density, body weight, and serum vitamin D and phosphorus concentrations varied with season. Bone mineral density, serum vitamin D concentration, and body weight also varied among individual alpacas. Serum vitamin D concentration was lower in January than the previous October and increased from May to the following September. The decrease in bone mineral density lagged behind the decrease in serum vitamin D concentration and was lower in May, compared with the previous October. Body weight was lower in May than the previous October or following September. Solar radiation was highest in July and lowest in December. CONCLUSIONS AND CLINICAL RELEVANCE: Seasonal changes in bone mineral density are associated with changes in serum vitamin D concentrations in alpacas. Changes in bone mineral density associated with a decline in serum vitamin D concentration may predispose some alpacas to developing fractures minimal trauma. 相似文献
968.
Long-term effect of chemical fertilizer,straw, and manure on labile organic matter fractions in a paddy soil 总被引:2,自引:0,他引:2
To assess the effect of long-term fertilization on labile organic matter fractions, we analyzed the C and N mineralization
and C and N content in soil, particulate organic matter (POM), light fraction organic matter (LFOM), and microbial biomass.
Results showed that fertilizer N decreased or did not affect the C and N amounts in soil fractions, except N mineralization
and soil total N. The C and N amounts in soil and its fractions increased with the application of fertilizer PK and rice straw.
Generally, there was no significant difference between fertilizer PK and rice straw. Furthermore, application of manure was
most effective in maintaining soil organic matter and labile organic matter fractions. Soils treated with manure alone had
the highest microbial biomass C and C and N mineralization. A significant correlation was observed between the C content and
N content in soil, POM, LFOM, microbial biomass, or the readily mineralized organic matter. The amounts of POM–N, LFOM–N,
POM–C, and LFOM–C closely correlated with soil organic C or total N content. Microbial biomass N was closely related to the
amounts of POM–N, LFOM–N, POM–C, and LFOM–C, while microbial biomass C was closely related to the amounts of POM–N, POM–C,
and soil total N. These results suggested that microbial biomass C and N closely correlated with POM rather than SOM. Carbon
mineralization was closely related to the amounts of POM–N, POM–C, microbial biomass C, and soil organic C, but no significant
correlation was detected between N mineralization with C or N amounts in soil and its fractions. 相似文献
969.
Prevalence of resistance to 11 antimicrobials among Campylobacter coill isolated from pigs on 80 grower-finisher farms in Ontario. 下载免费PDF全文
Norma P Varela Robert Friendship Cate Dewey 《Canadian journal of veterinary research》2007,71(3):189-194
We carried out a cross-sectional study to investigate antimicrobial resistance patterns of Campylobacter coli isolated from Ontario grower-finisher pigs. From January to June 2004, 1200 samples were collected from 80 farms by obtaining a constant number (15) of fecal samples per farm. Susceptibility of the isolates to 11 antimicrobial drugs was determined by the agar-dilution technique. The overall prevalence of resistance to 1 or more antimicrobials among the isolates was 99.2%. High levels of resistance were observed for azithromycin, clindamycin, erythromycin, streptomycin, and tetracycline: 91.7%, 82.5%, 81.4%, 70.7%, and 63.7%, respectively. For sulfamethoxazole, ampicillin, and nalidixic acid, resistance was observed in 40.3%, 26.6%, and 22.7% of the isolates, respectively. Although at very low levels, resistance was observed for ciprofloxacin (a fluoroquinolone), chloramphenicol, and gentamicin: in 2.4%, 1.7%, and 0.2%, respectively. Many of the isolates (29.7%) were resistant to 5 antimicrobials, the most common being azithromycin, clindamycin, erythromycin, streptomycin, and tetracycline. Isolates from the same farm showed at least 5 patterns of resistance. Results from this study indicate high levels of resistance to the antimicrobial drugs most commonly used in the Canadian swine industry (macrolides, lincosamides, and tetracyclines) among C. coli isolated from grower-finisher pigs in Ontario. Macrolides and fluoroquinolones are the drugs most commonly used to treat severe human campylobacteriosis. Fortunately, at present, there is little resistance to fluoroquinolones among C. coli from pigs in Ontario. 相似文献
970.
Chun-Lung Su Ian A. Gardner Wesley O. Johnson 《Journal of Agricultural, Biological & Environmental Statistics》2007,12(2):250-271
We develop Bayesian models to estimate cluster-level test characteristics, sensitivity, specificity, prevalence, and predictive
values, based on four different sampling schemes: a single test case and three sequential test cases. The corresponding cluster-level
characteristics are calculated and compared for different sample sizes, sampling schemes, individual-level sensitivities,
specificities, and cut-off values. We compared posterior estimates of individual-level and cluster-level characteristics for
these four sampling schemes with simulated data. Two illustrations, one for Johne’s disease in cattle and another for Salmonella
in pig herds, are used to demonstrate application of the methods. 相似文献