近41年西南地区极端温度变化趋势

利用西南地区90个气象台站1970-2010年逐日最高和最低温度资料,采用世界气象组织(WMO)定义的极端温度指数分析了西南地区近41a极端温度事件的时空变化、突变及周期特征.结果表明:近41年来在西南地区暖日数与冷日数具有较好的局部对称特征,暖夜数与冷夜数具有较高的对称性.暖指数近41年均表现为增多的趋势;冷指数主要为减少的趋势.暖日数与冷夜数分别在2004年与1994年前后发生了1次明显突变,冷日数突变出现在1997年,仅暖夜数未发生突变.暖指数与冷指数的周期振荡并不完全一致,但均存在1个4年的周期振荡.     

关于强遗传meso紧空间的等价刻画与Tychonoff乘积

首先证明:X是强遗传meso紧空间当且仅当X的每个开子空间的每个散射分解在X上有紧有限的开膨胀;然后推出关于强遗传meso紧空间的1个充要条件和1组等价刻画;最后获得了强遗传meso紧空间的1个Ty-chonoff乘积定理.     

一种基于序列数的关联规则挖掘算法

在关联规则挖掘研究中,为了在产生候选频繁项时减少算法存在的重复计算和冗余候选项,为了在计算支持数时减少扫描事务数据库的次数,提出了一种基于序列数的关联规则挖掘算法,其关联规则适合挖掘任何长度.该算法用事务属性的布尔约简法,将传统事务数据转换成二进制数,然后用数字的递增和递减两种方式双向搜索候选频繁项;算法通过序列数的度来计算支持数,实现一次扫描数据库,有效地提高了算法的效率.     

The pathogenesis of ovine footrot

Ovine footrot is a contagious and debilitating disease that is of major economic significance to the sheep meat and wool industries. The causative bacterium is the gram negative anaerobe Dichelobacter nodosus. Research that has used a classical molecular genetics approach has led to major advances in our understanding of the role of the key virulence factors of D. nodosus in the disease process. D. nodosus strains produce polar type IV fimbriae and extracellular serine proteases. Mutagenesis of the fimbrial subunit gene fimA and the pilT gene, which is required for fimbrial retraction, and subsequent testing of these mutants in sheep virulence trials has shown that type IV fimbriae-mediated twitching motility is essential for virulence. The extracellular protease genes aprV2, aprV5 and bprV have also been mutated. Analysis of these mutants has shown that ArpV5 is the major extracellular protease and that AprV2 is the thermostable protease that is responsible for the extracellular elastase activity. Structural analysis of AprV2 has revealed that it contains several novel loops, one of which appears to act as an exosite that may modulate substrate accessibility. Finally, virulence experiments in sheep have shown that the AprV2 protease is required for virulence.     

增铵营养对玉米生长发育的影响

测试了增铵营养对玉米生长发育的影响。利用盆栽,选用收敛型的玉米远征808和平展型的玉米四单19两个品种做试验材料,在氮素水平一致的条件下,设N03--N和NH4+-N比例分别是1∶O(处理1),2∶1(处理2).1∶1(处理3),1∶2(处理4),0∶1(处理5)及CK(不施氮)6个处理。玉米苗期后在生育期间每20d左右取样1次,测定株高、硝酸还原酶活性(NR)、叶绿素含量、叶面积、干物重,收获后烤种测产。结果不表明,增加根际NH4+-N可提高玉米叶绿素含量、干物质的积累量、提高子粒产量,且不同类型玉米对不同氮素形态比例需求不同,远征808在NO3-—N和NH4+—N比例为2∶1,四单19在NO3-—N和NH4+—N比例为1∶2时干物重和单株产量最高。     

Induction of B cell unresponsiveness to noninherited maternal HLA antigens during fetal life

Patients who have received many transfusions become highly sensitized and develop antibodies against almost all HLA alloantigens, so that finding a cross-match negative kidney donor is difficult. A survey of those patients showed that 50 percent did not form antibodies against the noninherited maternal HLA antigens. Apart from the obvious clinical implications, the data indicate that a human equivalent of murine neonatal or actively acquired tolerance has now been identified.     

川渝环状天然气管网运行能效评价

运行能效评价对于天然气管道运营企业提高节能降耗水平具有重要意义.为了对川渝环状天然气管网进行运行能效评价,在分析管网特点、能耗结构及运行能效评价特殊性的基础上,应用AHP层次分析原理,构建了4层次10指标天然气管网运行能效评价体系,综合能效指数的引入实现了以一个整合各种能耗因素影响力的综合能效指标对整个管网能效的定量评价.应用该指标体系对2012年2月川渝管网主环网运行能效进行评价,结果表明：19#、1#、18#、2#、16#站场的能效指数低于平均值,说明应加强站场能耗管理;上游1#～8#管段、中游9#～20#管段、下游21#～23#管段的平均综合能效指数分别为0.73、0.833、0.68,反映了川渝管网中游管段整体比上、下游管段能效高的现状,符合川渝管网无增压站的特点,评价结果可为川渝管网的能效管理和优化调配提供重要依据.     

8株具有生物活性的红树林真菌烟曲霉的分离、鉴定

对海南文昌清澜港红树林和广西北海红树林采集的植物组织和土壤样品进行真菌选择性分离,并对其发酵液进行抗金黄色葡萄球菌、抗白色念珠菌和细胞毒活性筛选。得到具有生物活性的真菌63株,通过培养特征、形态特征、ITS序列测定及其系统发育分析对菌株进行鉴定,发现其中有8株同为烟曲霉,占活性菌株的12.7%。综上表明,分离自红树林的烟曲霉部分菌株,产生了与其它生境烟曲霉菌株不一样的性质,具有了良好的生物活性。     

蜡梅鲜切花规范化生产技术探讨

从选地整畦、品种选择、引苗、定植、养护管理、采收等环节对蜡梅鲜切花规范化生产技术进行探讨,以期加强各地间的交流与合作、共促蜡梅产业持续健康发展。     

Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin

A novel toxin, NetB, has recently been identified in virulent avian Clostridium perfringens isolates and shown to be an essential virulence factor in a clinical necrotic enteritis isolate. To assess whether NetB is more generally associated with avian necrotic enteritis isolates we have screened a range of C. perfringens strains from geographically diverse locations for both the presence and expression of the netB gene. Forty-four isolates were derived from necrotic enteritis disease cases from Australia, Belgium, Denmark and Canada and 55 isolates from healthy chickens from Australia and Belgium. The majority of strains isolated from necrotic enteritis-affected birds were netB positive (70%) and there was an absolute correlation between the presence of netB and in vitro expression of the NetB protein. Only two of the C. perfringens isolates from healthy chickens carried netB. Sequencing of the netB gene from 23 positive isolates showed that NetB is highly conserved, with only one predicted amino acid (A168T) difference, in six isolates, compared to the published sequence. This change did not alter the in vitro activity of the NetB toxin. The gene encoding the recently discovered TpeL toxin was also screened using PCR and only found in a small proportion of NetB-positive isolates from diseased birds. A selection of NetB-negative isolates, originating from diseased birds, was unable to cause disease in a necrotic enteritis induction model. This study provides further evidence that NetB is important in pathogenesis and advances our current understanding of C. perfringens virulence factors in avian necrotic enteritis.