Soybean molasses as an organic carbon source in the farming of Litopenaeus vannamei (Boone, 1931) in a biofloc system

Soybean molasses was evaluated as a partial replacement for sugarcane molasses as a carbon source for biofloc development in the superintensive culture of Pacific white shrimp (Litopenaeus vannamei). A 50‐day study was conducted with juvenile (3.2 g) shrimp stocked in 16 800 L tanks at a stocking density of 250 shrimp m^?3. Control of total ammonia concentration was performed by the addition of combined mixtures of soybean and sugarcane molasses to the culture water. Three different molasses treatments were evaluated using different soybean‐to‐sugarcane molasses ratios: 15–85%, 38–62% and 60–40% respectively. The control group was treated only with sugarcane molasses. Water quality, chlorophyll a concentration, heterotrophic bacterial load, Vibrio spp. concentration and zootechnical indexes were all evaluated. Total ammonia concentration was controlled by heterotrophic and chemotrophic pathways. Biofloc formation, as quantified by measuring the total suspended solids, was not altered. The Vibrio spp. concentration showed a significant reduction in treatments with soybean‐to‐sugarcane molasses ratios of 38–62% and 60–40%. All combined mixtures of soybean and sugarcane molasses could maintain water quality and productivity in the superintensive culture of L. vannamei using the biofloc system. Thus, the potential use of a residue from agroindustry as a carbon source in a biofloc culture is demonstrated.     

稻草及其制备的生物质炭对土壤团聚体有机碳的影响

向土壤中添加生物质炭已被认为是改善土壤质量,增加碳吸存的有效措施。通过模拟实验,利用同位素δ13C标记技术,研究稻草及其制备的生物质炭添加对土壤团聚体有机碳的影响。结果表明：稻草和生物质炭对土壤团聚体中新形成碳和原有机碳的影响截然不同。培养112 d,来自稻草或生物质炭的新碳主要进入到中团聚体（50 ~ 250 μm）中,比例为70.3% ~ 75.3%。与对照土壤相比,稻草添加显著促进了大团聚体（250 ~ 2 000 μm）原有机碳的分解（p <0.05）,但对中团聚体和微团聚体（<50 μm）原有机碳的影响并不明显,而生物质炭添加（SB250和SB350）则对大团聚体和中团聚体原有机碳没有显著影响,但SB250处理（土壤中加入250℃热解制备的生物质炭）显著抑制了微团聚体原有机碳的分解（p <0.05）,而SB350处理（土壤中加入350℃热解制备的生物质炭）的则无影响。对于同一粒级团聚体,稻草与生物质炭处理的区别,主要体现在新碳分配上,而对原有机碳的影响并不显著。     

秦巴山区土地利用时空格局及地形梯度效应

自然景观的垂直地带性为区域土地利用的垂直景观格局奠定了基础,同时也决定了垂直景观带上土地利用的强度及其变化情况。该文采用地形位指数、分布指数、土地利用程度综合指数和土地利用信息图谱法等,分析了1989-2010年秦巴山区土地利用格局的地形梯度效应和图谱信息,揭示地形控制下土地利用时空格局和土地转移的变化规律。结果表明:1)秦巴山区土地利用以林地和耕地为主,呈西林东耕分布;1989-2010年间林地减少显著,建设用地增加明显,其中耕地是建设用地主要转入来源,林、草地是耕地主要转入来源。2)秦巴山区各生态亚区用地类型差异明显,各生态亚区土地利用转移方向也存在差异性。3)秦巴山区土地利用强度指数呈东高西低,中高周低的态势;土地利用强度变化率稳定少动,增加趋势不明显;土地利用动态度呈中高周低的态势,整体变化不大。4)水域、建设用地和耕地主要集中在地形起伏度小和坡度小的低地形位,且耕地主要转移为其他两种用地类型,建设用地有向中低地形位分布的趋势。林地和草地集中在地形起伏大、坡度陡的中高地形位,且存在着相互转移演替现象,中低地形位往往是耕地、林地、草地间的相互转换,未来势必加强坡耕地和低山丘陵区林草地的管治工作。     

基于GIS的长江中游油菜种植区土壤养分及pH状况

为了掌握长江中游油菜种植区土壤养分状况,在湖北、湖南、江西三个省份调查和取样分析5 463份土壤数据的6个指标(土壤有机质、全氮、有效磷、速效钾、有效硼和pH),基于Arc GIS平台,采用Kriging插值法分析4个不同油菜种植区域(二熟制山地区、二熟制平原区、三熟制山地区、三熟制平原区)的土壤养分状况。结果表明,长江中游油菜种植区土壤有机质、全氮和有效磷均处于适宜/丰富的含量水平,基本没有5级和6级水平的有机质、全氮、有效磷分布。从不同区域来看,以上3个养分指标总体均表现为三熟制地区养分含量高于二熟制地区。长江中游仍有1.82×10~5 hm~2区域土壤速效钾处于缺乏的水平(30~50 mg kg~(-1))。土壤有效硼含量仍有待提高,尤其是在三熟制地区,土壤有效硼缺乏(5级和6级)的比例仍高达13.6%~20.4%。土壤pH多集中在6.0左右,但是三熟制地区仍然有较高比例(约35.7%)的土壤pH处于4.5~5.5。综上所述,长江中游不同种植区域土壤养分性状存在差异,尤其是三熟制地区仍有相当比例需要提高土壤有效硼和改良酸性土壤。     

生物降解膜促进冬油菜养分吸收减少土壤硝态氮累积

针对普通地膜覆盖导致的农田环境污染和土地退化问题,通过2 a田间试验,从土壤有机质含量、硝态氮累积与分布、作物养分吸收和籽粒产量等层面出发,进行了普通地膜覆盖(PM)、生物降解地膜覆盖(JM)和露地(CK)栽培冬油菜的对比研究。结果表明,播种后60和150 d,JM处理的土壤有机质含量、土壤硝态氮的累积和分布与PM处理无显著差异;播种后240 d,JM处理的土壤有机质含量显著大于PM处理,土壤硝态氮的累积量显著小于PM处理,且PM处理土壤硝态氮的淋洗下移峰值更大。PM和JM处理冬油菜的产量及地上部各器官的氮、磷、钾吸收量均显著大于CK,且PM和JM无显著差异。与PM处理相比,JM处理在播种后240 d时土壤有机质质量分数提高7.0%,土壤硝态氮累积量减少34.1%。可见,PM处理在冬油菜生育后期过分消耗地力,且残留在土壤中的硝态氮含量较高。该研究从土壤营养和作物养分吸收利用方面为生物降解地膜应用于农业生产的可行性提供了理论依据。     

Weed abundance and soil seedbank responses to tillage systems in continuous maize crops

Weed abundance in crops undergoes frequent changes, often due to changes in tillage practices. Annual species, with quick germination, a short vegetative stage, profuse seed production and long-lived seeds become problematic under zero-tillage systems. Portulaca oleracea L. and Amaranthus blitoides L. are widespread weeds in the Mediterranean area, prominent in irrigated crops. We studied the total weed abundance in the field, and specifically these two species (Portulaca oleracea and Amaranthus blitoides) with high frequency of occurrence in monoculture maize, from 2012 to 2014, in the field and soil seedbank. Results showed significant differences between zero-tillage (ZT) and conventional tillage (CT) systems on total weed abundance and relative abundance of Portulaca oleracea. Total weed abundance decreased in ZT plots (from 136 plants m^?2 to 25 and 46 plants m^?2, in 2013 and 2014 respectively). The same trend was observed in Portulaca oleracea recorded in ZT plots, but the abundance of Amaranthus blitoides did not vary in this system. Weed seedling germination and weed seed numbers both of total weed seedbank and Portulaca oleracea, were greater in ZT plots compared to CT, regarding Amaranthus blitoides seedling germination and seed count, the values did not increase with ZT, in continuous maize crops.     

模拟条件下土壤硝化作用及硝化微生物对不同水分梯度的响应

以江苏滨海县一植稻土壤为研究对象,在微宇宙培养条件下设置了不同水分处理(最大持水量的30%、60%、90%和淹水2 cm深),研究了硝化作用及硝化微生物对水分变化的响应特征。结果表明:淹水处理显著降低了土壤的氧化还原电位(Eh),但所有处理土壤Eh变化范围为330~500 m V,土壤整体处于氧化态。在每7天向土壤加入10 mg kg-1NH+4-N的连续培养过程中,各个水分处理均观察到明显的NH+4-N降低和NO-3-N累积的现象,60%WHC处理下土壤硝态氮累积最显著和迅速,90%WHC处理次之,随培养时间延长,30%WHC和淹水处理也观察到明显的硝化作用。淹水处理中氨氧化细菌(Ammonia-oxidizing bacteria,AOB)的数量显著高于非淹水处理,且淹水处理中AOB在DGGE图谱上的条带更加清晰明亮,而氨氧化古菌(Ammonia-oxidizing archaea,AOA)的群落组成和数量在不同水分处理间无明显变化。表明该土壤中AOB对水分条件变化响应灵敏,是该土壤的硝化作用、尤其是淹水条件下硝化作用发生的主要原因。     

不同前处理条件对动态光散射检测酪蛋白胶束粒径的影响

为了准确评价乳的稳定性和加工性能,探讨不同前处理条件对动态光散射检测酪蛋白胶束粒径的影响,研究了稀释液的种类(超纯水、钙咪唑缓冲液、模拟牛乳超滤液和牛乳超滤液)、稀释液温(4和25℃)和稀释液的放置时间(0~48 h)对脱脂乳中酪蛋白胶束粒径的动态光散射测试结果的影响,并将酪蛋白胶束粒径的动态光散射测试结果与冷冻透射电镜图像中测得的真实结果进行比较。研究发现以超纯水和钙咪唑缓冲液作为脱脂乳稀释液时,部分胶束发生解离,影响测试结果;采用牛乳超滤液及模拟牛乳超滤液作为稀释液时,胶束的微环境没有改变,反映了胶束的真实粒径及分布;放置24 h后,牛乳超滤液及模拟牛乳超滤液将产生颗粒;温度对测试有显著的影响(P0.05):4℃的样品用25℃的稀释液进行稀释后,动态光散射的计数率和粒径分别增大了16.6%和11.4%;25℃的样品用4℃的稀释液进行稀释后,计数率和粒径分别降低了16.1%和9.8%。结果表明酪蛋白胶束粒径的测试前处理较适宜的条件为:在与样品的温度相同条件下,以配置好后24 h内的模拟牛乳超滤液或牛乳超滤液(10 k Da超滤膜)作为脱脂乳的稀释液进行稀释。通过与冷冻电镜条件下测得的酪蛋白胶束粒径的真值比较,发现该前处理条件下酪蛋白胶束粒径的动态光散射测试结果的相对误差为-5.7%~1.8%,表明该样品前处理方法可用于动态光散射方法快速检测酪蛋白胶束粒径。研究结果为快速、准确地获取酪蛋白胶束的粒径信息,进而准确分析乳的稳定性及加工性能提供参考。     

Xylan-rich hemicelluloses-graft-acrylic acid ionic hydrogels with rapid responses to pH, salt, and organic solvents

Exploitation of biomaterials derived from renewable resources is an important approach to address environmental and resource problems in the world today. In this paper, novel ionic hydrogels based on xylan-rich hemicelluloses were prepared by free radical graft copolymerization of acrylic acid (AA) and xylan-rich hemicelluloses (XH) by using N,N-methylene-bis(acrylamide) (MBA) as cross-linker and ammonium persulfate/N,N,N',N'-tetramethylethylenediamine (APS/TMEDA) as redox initiator system. The network characteristics of the ionic hydrogels were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), as well as by determination of mechanical properties, swelling, and stimuli responses to pH, salts, and organic solvents. The results showed that an increase in the MBA/XH or AA/XH ratio resulted in higher cross-linking density of the network and thus decreased the swelling ratio. Expansion of the network hydrogels took place at high pH, whereas shrinkage occurred at low pH or in salt solutions as well as in organic solvents. The ionic hydrogels had high water adsorption capacity and showed rapid and multiple responses to pH, ions, and organic solvents, which may allow their use in several areas such as adsorption, separation, and drug release systems.     

自动观测与人工观测土壤温度差异及分析

利用我国基本(准)站在人工观测与自动观测双轨运行期间的平行观测资料,分析了不同观测方式下各层土壤温度的差异.结果表明,全国平均自动与人工观测各层土壤温度的日对比差值均在0.11℃以下,且日对比差值随着深度的增加而减小.从5 cm到320 cm,无论冬季还是夏季,全国绝大部分地区平均对比差值在±0.2℃之间.由浅至深各层土壤温度日对比差值的标准差从0.78℃减小至0.47℃,其不确定度在5～ 20 cm时均超过了1.0℃,但在深层(40 cm及以下),不确定度已经低于1.0℃.除极个别站外,各层自动与人工观测日平均值无显著性差异.自动与人工观测土壤温度的差异主要是由于土壤中水平温度场分布非均匀和土壤垂直温度梯度很大及对比观测时间不同步造成的.