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101.
The aim of this study was to develop regression models for correlation of canine fetal heart development with body size to characterize normal development or suggest cardiac anomalies. Twenty clinically healthy pregnant bitches, either brachycephalic and non-brachycephalic, were examined ultrasonographically. Transabdominal fetal echocardiography was conducted every 4 days from the beginning of cardiac chambers differentiation until parturition. Ten cardiac parameters were measured: length, width and diameter of the heart; heart area; left and right ventricular dimensions; left and right atrial dimensions; and aortic and pulmonary artery diameter. Femoral length, biparietal diameter and abdominal cross-sectional area were also recorded. Regression equations were developed for each parameter of fetal body size, and linear and logarithmic models were compared. The model with the highest correlation coefficient was chosen to produce equations to calculate relative dimensions based on the correlations. Only the left-ventricular chamber differed between the two racial groups. Biparietal diameter was the independent parameter that produced the highest correlation coefficient for the most fetal cardiac dimensions, although good correlations were also observed using femoral length and abdominal cross-sectional area. Heart width and heart diameter were used as surrogates of cardiac development, as these measurements showed the best statistical correlation. Quantitative evaluation of fetal cardiac structures can be used to monitor normal and abnormal cardiac development.  相似文献   
102.
Tendons regenerate poorly due to a dense extracellular matrix and low cellularity. Cellular therapies aim to improve tendon repair using mesenchymal stem cells and tenocytes; however, a current limitation is the low proliferative potential of tenocytes in cases of severe trauma. The purpose of this study was to develop a method useful in veterinary medicine to improve the differentiation of Peripheral Blood equine mesenchymal stem cells (PB-MSCs) into tenocytes. PB-MSCs were used to study the effects of the addition of some growth factors (GFs) as TGFβ3 (transforming growth factor), EGF2 (Epidermal growth factor), bFGF2 (Fibroblast growth factor) and IGF-1 (insulin-like growth factor) in presence or without Low Level Laser Technology (LLLT) on the mRNA expression levels of genes important in the tenogenic induction as Early Growth Response Protein-1 (EGR1), Tenascin (TNC) and Decorin (DCN). The singular addition of GFs did not show any influence on the mRNA expression of tenogenic genes whereas the specific combinations that arrested cell proliferation in favour of differentiation were the following: bFGF2 + TGFβ3 and bFGF2 + TGFβ3 + LLLT. Indeed, the supplement of bFGF2 and TGFβ3 significantly upregulated the expression of Early Growth Response Protein-1 and Decorin, while the use of LLLT induced a significant increase of Tenascin C levels. In conclusion, the present study might furnish significant suggestions for developing an efficient approach for tenocyte induction since the external administration of bFGF2 and TGFβ3, along with LLLT, influences the differentiation of PB-MSCs towards the tenogenic fate.  相似文献   
103.
104.

Background

The pathogenic mechanism of equine recurrent uveitis (ERU) is still poorly defined and many variations between experimental animal models and spontaneous disease exist.

Objectives

The aim of our study was to investigate if Th17 cell-mediated response plays role in the pathogenesis of the used experimental model in horses and to reveal its pathological findings.

Methods

Experimental uveitis was induced in 6 healthy horses. The concentrations of retinal autoantigen CRALBP and IL-17 were measured using ELISA in aqueous humor and vitreous body of the 12 inflamed eyes as well as in 12 control non-inflamed eyes taken from 6 horses in slaughter house. After centrifugation of the two eye media, smears were prepared and cytological investigation was performed. Tissue specimens were taken from all eye globes and were submitted to histopathological investigation.

Results

CRALBP and IL-17 concentrations were significantly elevated in eye media of horses with experimental uveitis in comparison with controls. Cytological and histopathological findings corresponded to the changes characteristic of chronic immune-mediated inflammation with mononuclear cell infiltration of uvea, choroid, retina, and eye media as well as severe retinal destruction.

Conclusions

Our study demonstrated the involvement of the retinal autoantigen CRALBP as well as IL-17 in the pathogenesis of experimental uveitis in horses. These findings suggests that this experimental uveitis in horses may serve as a suitable animal model for investigation of IL-17- mediated immune response during spontaneous autoimmune uveitis in horses as well as in humans.
  相似文献   
105.
Symmetrical alopecia, scaling and hepatitis in a rabbit   总被引:2,自引:1,他引:1  
A 5-year-old rabbit with inappetence, symmetrical alopecia and skin lesions was examined. No mites or Malassezia were found in skin scrapings and tape impressions and dermatophyte culture was negative. Trial therapy with ivermectin did not reduce skin lesion severity, and euthanasia was performed because of anorexia after 1 month. Histopathology of the skin showed hyperkeratosis, lymphocytic exocytosis, cell-poor interface dermatitis (lymphocytic infiltration and apoptotic cells in basal layer of epidermis), absence of sebaceous glands and lymphocytic mural folliculitis comparable to sebaceous adenitis and thymoma-associated exfoliative dermatitis previously described in rabbits. The liver exhibited an interface hepatitis, comparable to autoimmune hepatitis in man. The occurrence of morphological similarities to exfoliative dermatitis and sebaceous adenitis in rabbits, in association with an autoimmune hepatitis, has not been described before.  相似文献   
106.
107.
本试验旨在研究苏氨酸和异亮氨酸对皖西白鹅屠宰性能、器官指数及肠道形态结构、消化酶活性、抗氧化和免疫指标的影响。选取30日龄体重相近的皖西白鹅180只,随机分为3组,每组3个重复,每个重复20只。对照组饲喂基础饲粮,苏氨酸组在基础饲粮中添加2.0 g/kg的L-苏氨酸,异亮氨酸组在基础饲粮中添加2.5 g/kg的L-异亮氨酸。试验期60 d。结果表明:1)各组之间90日龄屠宰性能和器官指数无显著差异(P>0.05)。2)异亮氨酸组60日龄十二指肠和空肠绒毛高度、隐窝深度显著或极显著高于对照组(P<0.05或P<0.01),苏氨酸组60日龄十二指肠、空肠和回肠隐窝深度及90日龄十二指肠和回肠绒毛高度显著或极显著高于对照组(P<0.05或P<0.01)。3)苏氨酸组和异亮氨酸组60、90日龄十二指肠胰蛋白酶活性极显著高于对照组(P<0.01),苏氨酸组60、90日龄十二指肠麦芽糖酶活性及90日龄空肠淀粉酶、乳糖酶活性显著或极显著高于对照组(P<0.05或P<0.01)。4)异亮氨酸组60日龄空肠总抗氧化能力显著高于对照组和苏氨酸组(P<0...  相似文献   
108.
小麦红吸浆虫在土内有4种虫态,其分布以中层(7~13厘米)最多,下层(13~20厘米)次之,上层(0~7厘米)最少,20~38厘米仍有虫口分布.幼虫以上层为最多,中层次之、下层最少;圆茧以中,下层最多,上层很少;长茧及蛹以上层为最多,中、下层较少.成虫发生迟早及持续时间长短的关键因素是3~5月的降雨量,其中以4月下旬降雨最为重要.4月下旬降雨多,则成虫发生早,持续时间长,成虫发生盛期与小麦抽穗期相吻合的机会多,小麦受害重.在小麦抽穗前施一次长效农药封闭地面,可杀死不断上升地面的幼虫和蛹,阻止成虫羽化.  相似文献   
109.
A new Monte Carlo Method for system reliability evaluation is proposed in this paper. The repeated sampling of system states with relatively great probabilities is avoided in the simulation,thus the sampling efficiency is significantly improved. Besides,the negative correlations used in different trials can further reduce the estimated varinaces. The sparse matrix technique is employed to reduce the memory requirement and to save the computer time. Comparative studies show that the method proposed is of great value and the application to the IEEE reliability test system (power system) is successful.  相似文献   
110.
A flexible mathematical model used for dynamic simulation studies of HVDC systems which is based on the stale variable method is presented, and the correspongding computer program has been implemented. Generality, easy to use, and suitable to run on microcomputers are the main features of the model and the computer program. Therefore the model is easy to be extended for application. An example is given in the paper to verily the coneclness of the model and the program.  相似文献   
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