Ronidazole pharmacokinetics after intravenous and oral immediate-release capsule administration in healthy cats

Ronidazole (RDZ) is an effective treatment for feline Tritrichomonas foetus infection, but has produced neurotoxicity in some cats. An understanding of the disposition of RDZ in cats is needed in order to make precise dosing recommendations. Single-dose pharmacokinetics of intravenous (IV) RDZ and immediate-release RDZ capsules were evaluated. A single dose of IV RDZ (mean 9.2mg/kg) and a 95mg immediate-release RDZ capsule (mean 28.2mg/kg) were administered to six healthy cats in a randomized crossover design. Plasma samples were collected for 48 h and assayed for RDZ using high pressure liquid chromatography (HPLC). Systemic absorption of oral RDZ was rapid and complete, with detection in the plasma of all cats by 10 min after dosing and a bioavailability of 99.64 (±16.54)%. The clearance of RDZ following IV administration was 0.82 (±0.07) ml/kg/min. The terminal half-life was 9.80 (±0.35) and 10.50 (±0.82) h after IV and oral administration, respectively, with drug detectable in all cats 48h after both administrations. The high oral bioavailability of RDZ and slow elimination may predispose cats to neurotoxicity with twice-daily administration. Less frequent administration should be considered for further study of effective treatment of T foetus-infected cats.     

Surgical decompression,with or without adjunctive therapy,for palliative treatment of primary vertebral osteosarcoma in dogs

Vertebral osteosarcoma (OSA) is the most common primary vertebral tumor in dogs, however studies examining the survival time after surgical decompression of these tumors are limited. There is also limited information regarding the benefit of adjunctive treatments such as radiation therapy or chemotherapy in these patients. The goal of this study was to determine survival time of dogs with primary vertebral OSA after palliative decompressive surgery alone and combined with radiation therapy and/or chemotherapy. Records from 22 client‐owned dogs diagnosed with primary vertebral OSA and treated with decompressive surgery were collected retrospectively from eight referral institutions. Survival time was assessed for dogs treated with surgery alone as well as dogs who received adjunctive radiation therapy and/or chemotherapy. Median survival time in the 12 dogs treated with surgery alone was 42 days (range: 3‐1333 days). The three dogs treated with surgery and chemotherapy had a median survival time of 82 days (range: 56‐305 days). Only one dog was treated with surgery and radiation therapy; this dog survived 101 days. Six dogs were treated with surgery, radiation therapy and chemotherapy; these dogs had a median survival time of 261 days (range: 223‐653 days). Cause of death in all cases that survived the initial postoperative period was euthanasia secondary to confirmed or suspected tumor regrowth. The results of this study suggest that definitive radiation therapy, possibly combined with concurrent chemotherapy, significantly improves survival in dogs treated with palliative decompressive surgery for vertebral OSA and should be the treatment of choice in selected cases.     

The role of ear environment in postharvest susceptibility of maize to toxigenic Aspergillus flavus

A kernel screening assay (KSA) was used to assess the genetic and environmental effects on the vulnerability of maize to aflatoxin accumulation. Kernels of 26 inbred lines that had been grown in seven environments, and 190 lines of the Intermated B73xMo17 (IBM) population grown in one location in the United States, were inoculated with a toxigenic strain of A. flavus and incubated in the dark at 30°C for 6 days. Percent kernel colonization (PKC), sporulation and aflatoxin were influenced by the maize genotypes (G), the location (“ear environment” or E) and the GxE interactions. Overall, low broad‐sense heritabilities were observed for PKC, sporulation and aflatoxin. PKC was significantly correlated with sporulation in all environments. Aflatoxin was positively correlated with colonization for two and with sporulation for all ear environments. Higher grain sulphur or magnesium in IBM was associated with less colonization or aflatoxin. Postharvest susceptibility of maize to aflatoxin is thus influenced by factors that are modulated by the ear environment. In a KSA, sporulation could be a proxy test for aflatoxin accumulation.     

Discovery and directed evolution of a glyphosate tolerance gene

The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity. Kinetic properties of the discovered enzymes were insufficient to confer glyphosate tolerance to transgenic organisms. Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude from 0.87 mM-1 min-1 to 8320 mM-1 min-1. From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize. Glyphosate acetylation provides an alternative strategy for supporting glyphosate use on crops.     

Relationships of dead wood patterns with biophysical characteristics and ownership according to scale in Coastal Oregon,USA

Dead wood patterns and dynamics vary with biophysical factors, disturbance history, ownership, and management practices; the relative importance of these factors is poorly understood, especially at landscape to regional scales. This study examined current dead wood amounts in the Coastal Province of Oregon, USA, at multiple spatial scales. Objectives were to: (1) describe current regional amounts of several characteristics of dead wood; (2) compare dead wood amounts across ownerships; (3) determine the relative importance, according to spatial scale, of biophysical and ownership characteristics, to regional dead wood abundance. Dead wood plot data were evaluated with respect to explanatory variables at four spatial scales of resolution: plots, subwatersheds, watersheds and subbasins. The relationships of dead wood characteristics with biophysical attributes and ownership were diverse and scale-specific. Region-wide dead wood abundance and types varied among ownerships, with public lands typically having higher amounts of dead wood and more large dead wood than private lands. Regression analysis of total dead wood volume indicated that ownership was important at the subbasin scale. Growing season moisture stress was important at plot, subwatershed, and watershed scales. Topography was important at the two coarser scales. Multivariate analysis of dead wood gradients showed that ownership was important at all scales, topography at the subbasin scale, historical vegetation at watershed and subbasin scales, and current vegetation at plot and subwatershed scales. Management for dead wood and related biodiversity at watershed to landscape scales should consider the distinct dynamics of snags and logs, the importance of historical effects, and the relevance of ownership patterns.     

Is risk consistent across tier‐based harvest control rule management systems? A comparison of four case‐studies

There can be substantial differences in data quality and quantity among fished species. Consequently, the quality and type of assessments can also vary substantially. However, all species, especially those that are targeted, need to be managed. Several jurisdictions have developed hierarchical tier systems that categorize stocks based on, for example, the data available for assessment purposes and/or the extent to which quantities on which management advice is based can be estimated. Four case‐studies (Australia's Southern and Eastern Scalefish and Shark Fishery, the USA west coast groundfishery, the USA Alaskan crab fishery and EU fisheries) are used to contrast the types of hierarchical tier systems available, and to assess the extent to which each system constrains risk to be equivalent among the tiers (termed risk equivalency). Only the Australian system explicitly aims to achieve risk equivalency. However, this intent has not been fully operationalized. Our review reveals that best practice is not to define tiers simply on data availability, but also on what the assessments based on those data are capable of estimating. In addition, clearly differentiating the quantification of uncertainty from how decision‐makers wish to address that uncertainty would simplify justification of buffers (the gap between the assessment‐produced target catch or effort and the final management decision that accounts for uncertainty and risk). Risk equivalency can be achieved using management strategy evaluation to select the values for control variables, which determine the buffer given the uncertainty associated with the assessment.     

Development of cotton pests exhibiting different feeding strategy on water-stressed and kaolin-treated cotton plants

Consistent data demonstrate the positive response of sap-sucking insects to water-stressed plants, but there is a lack of information about the performance of chewing species, including whether their responses vary according to their feeding specializations. We tested the hypothesis that herbivores with distinct feeding strategies and host specialization will respond differently to two plant conditions, i.e. water deficit and kaolin treatment, where the latter is used to reduce abiotic and biotic plant stressors. We determined the development and reproduction of four major cotton pest species with different feeding strategies (chewing and sap sucking) and with specializations ranging from monophagous to polyphagous using well-watered or water-stressed cotton plants, which were treated or not treated with kaolin. Three chewing (lepidopteran) species, Alabama argillacea (Hüb.), Heliothis virescens (Fabr.) and Chrysodeixis includens (Walk), as well as one sap-sucking species, Bemisia tabaci (Gen.), were used as models. The neonate larvae of all three chewing species exhibited lower survival when fed the leaves of water-stressed plants. In addition, the neonate larvae fed leaves from water-stressed and kaolin-treated plants did not complete their development or they exhibited low survival. Older larvae also exhibited significantly lower survival on kaolin-treated plants. The effects on life history traits were more obvious in monophagous chewing species compared with polyphagous species when fed leaves from water-stressed plants. The performance of B. tabaci was superior on water-stressed and untreated kaolin plants. Thus, water-stressed and kaolin-treated plants negatively affected all chewing species, whereas water-stressed plants only positively affected the sap-sucking species.