A protocol for the assay of arylesterase activity in soil

We set up a protocol for the assay of the arylesterase activity, using p-nitrophenyl acetate (p-NPA) as substrate, dimethylsulfoxide as solvent, modified universal buffer at pH 7.5, and determination of the reaction product (p-nitrophenol) after separation of non-hydrolysed p-NPA after reaction, and tested it using eight soils with a wide range of characteristics. Various incubation temperatures and times, pH values and substrate concentrations were also used to find the optimal conditions for the enzyme activity and to determine characteristics and kinetic parameters of soil arylesterase. Arylesterase activity was significantly correlated with total organic C, total N, and soil ATP content. Soil arylesterase activity showed a pH optimum at 7.5, optimal temperature between 55 and 65 °C and linear increase with incubation time. The K_m values ranged from 4.3 to 8.5 mM, the V_max values from 326 to 803 μmol p-NP g⁻¹ h⁻¹, with higher K_m values observed in soils with higher organic matter content. We conclude that the proposed assay protocol is suitable to determine the arylesterase activity in a wide range of soils.     

Separation of Delta5- and Delta7-phytosterols by adsorption chromatography and semipreparative reversed phase high-performance liquid chromatography for quantitative analysis of phytosterols in foods

A method for the separation, isolation, and identification of phytosterols was developed. A commercial phytosterols mixture, Generol 95S, was fractionated first by adsorption silica gel column chromatography and then separated by means of a semipreparative reverse phase high-performance liquid chromatography fitted with a Polaris C8-A column (250 mm x 10 mm i.d., 5 microm) using isocratic acetonitrile:2-propanol:water (2:1:1, v/v/v) as the mobile phase. Milligram scales of six individual phytosterols, including citrostadienol, campesterol, beta-sitosterol, Delta7-avenasterol, Delta7-campesterol, and Delta7-sitosterol, were obtained. Purities of these isolated sterols were 85-98%. Relative response factors (RRF) of these phytosterols were calculated against cholestanol as an authentic commercial standard. These RRF values were used to quantify by gas chromatography-mass spectrometry (GC-MS) the phytosterols content in a reference material, oils, and chocolates.     

Cover Image,Volume 73, Issue 2

The cover image, by Eugênio E. Oliveira et al., is based on the Research Article Pyrethroid resistance is associated with a kdr‐type mutation (L1014F) in the potato tuber moth Tecia solanivora, DOI: 10.1002/ps.4414 . Photo Credit: Edison Torrado‐Leon.

     

The use of mobile devices with multi-tag technologies for an overall contextualized vineyard management

This paper describes a Viticulture Service-Oriented Framework (VSOF) which turns around context elements or tags that are placed in the field and which can be decoded by mobile devices such as mobile phones or PDAs. The tags are used to automatically associate a field location to the relevant database tables or records and also to access contextual information or services. By pointing a mobile device to a tag, the viticulturalist may download data such as climatic data or upload information such as disease and pest incidence in a simple way, without having to provide coordinates or any other references, and without having to return to a central office. This work is part of an effort to implement a large-scale distributed cooperative network in the Douro Demarcated Region in Northeast Portugal, a region in which the effort makes particular sense due to the extremely variable topography and mesoclimates. The possibility of exchanging contextualized information and accessing contextualized services in the field, using well-known devices such as cell phones, may contribute to increase the rate of adoption of information technology in viticulture, and contribute to more efficient and closer-to-the-crops practices.