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121.
122.
C Yamada WB Feitosa R Simões AC Nicacio CM Mendes MEOA Assumpção JA Visintin 《Reproduction in domestic animals》2011,46(1):173-176
The current study examined the protective effects of l ‐glutamine and cytochalasin B during vitrification of immature bovine oocytes. Oocyte vitrification solution (PBS supplemented with 10% FCS, 25% EG, 25% DMSO and 0.5 m trehalose) was the vitrification control. Treatments were the addition of 7 μg/ml cytochalasin B, 80 mm glutamine or both cytochalasin and glutaminine for 30 s. After warming, oocytes were matured in vitro for 24 h, fixed and stained with Hoechst (33342) for nuclear maturation evaluation. l ‐glutamine improved the vitrified/warmed immature bovine oocytes viability (32.8%), increasing the nuclear maturation rates compared to other treatments and the no treatment vitrified control (17.4%). There was, however, no effect of cytochalasin B on in vitro maturation (14.4%). 相似文献
123.
Sharma Umesh Bhardwaj Daulat Ram Sharma Sunny Sankhyan Neeraj Thakur Chaman Lal Rana Neerja Sharma Subhash 《Agroforestry Systems》2022,96(5-6):925-940
Agroforestry Systems - Agroforestry as a smart agriculture practice has started to get global attention nowadays. The present investigation is based on bamboo-ginger intercropping research carried... 相似文献
124.
Some of the factors influencing the propagation of caper (Capparis spinosa L.) plants in vitro and germination of the seed were studied. The number of adventitious shoots emerging from caper stems cultured in vitro increased from 2.2 shoots per explant when the growth medium contained 2 mg/L of gibberellic acid (GA3) to 5.5 when the growth medium contained 2 mg/L zeatin riboside (ZR) and 1 mg/L naphthalene acetic acid (NAA). The best medium for callus formation from leaf and stem parts contained the growth regulators 1 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L NAA and the best medium for plant regeneration contained 1 mg/L kinetin and 0.1 mg/L indole-3-acetic acid (IAA). The effect of gamma irradiation on the growth of caper shoots in vitro was also studied. A 10 Gy dose of gamma irradiation stimulated growth of shoots up to 200% and increased shoot rooting percentage from 75 to 100%. 相似文献
125.
Influence of socio-economic and cultural factors in rice varietal diversity management on-farm in Nepal 总被引:1,自引:0,他引:1
Ram Bahadur Rana Chris Garforth Bhuwon Sthapit Devra Jarvis 《Agriculture and Human Values》2007,24(4):461-472
A questionnaire survey of 408 households explored the role of socio-economic and cultural factors in rice (Oryza sativa L.) varietal diversity management on-farm in two contrasting eco-sites in Nepal. Multiple regression outputs suggest that
number of parcels of land, livestock number, number of rice ecosystems, agro-ecology (altitude), and use of chemical fertilizer
have a significant positive influence on landrace diversity on-farm, while membership in farmers’ groups linked to extension
services has significant but negative influence on landrace diversity. Factors with significant positive influence on diversity
of modern varieties on-farm were number of parcels of land and of rice ecosystems, access to irrigation, membership in farmers’
groups, and use of insecticide. Within communities, resource-endowed households maintain significantly higher varietal diversity
on-farm than resource-poor households and play a significant role in conserving landraces that are vulnerable to genetic erosion
and those with socio-cultural and market-preferred traits. Resource-poor households also contribute to local diversity conservation
but at lower richness and area coverage levels than resource-endowed households. Households where a female had assumed the
role of head of household due to death or migrant work of her husband had less diversity due to lower labor availability.
Landraces with socio-cultural and market-preferred traits are few in number but have potential to be conserved on-farm.
相似文献
Ram Bahadur RanaEmail: |
126.
AC Fabian 《Science (New York, N.Y.)》1997,275(5296):48-49
The soft x-ray spectra of the Virgo and Coma clusters cannot be modeled by emission from the well-known hot intracluster medium attenuated by photoelectric absorption along the line of sight in our galaxy. If the excess soft emission is due to thermal emission in the clusters from gas at about 1 million kelvin, then the gas should be rapidly cooling. The high rate of cooling poses problems for the origin of the gas and its sink. A model in which the component is due to turbulent mixing layers around clouds scattered throughout the intracluster medium may explain the excess emission. The gas in the mixing layer is reheated after cooling, so that the total mass remains relatively small. 相似文献
127.
Mubshar Hussain Rana Qaisar Abbas Muhammad Faizan Ashraf Muhammad Shahzad Muhammad Farooq 《Journal of plant nutrition》2013,36(18):2330-2343
AbstractNitrogen use efficiency (NUE) is low in cereals especially in wheat. Different wheat cultivars may vary in NUE due to inherited biological nitrification inhibition (BNI) potential. In this study, three wheat cultivars (Punjab-2011, ARRI-2011 and Millat-2011) were fertilized at the rate of 140?kg ha?1 with three N sources [nitrophos (NP), urea and calcium ammonium nitrate (CAN)]. The soil nitrate (NO3?)-N contents were significantly enhanced coupled with simultaneous decrease in ammonium (NH4+)-N contents in the rhizosphere of cultivar Punjab-2011, fertilized with NP; however, cultivar Millat-2011 receiving urea behaved in contrast. Wheat cultivar Punjab-2011 fertilized with NP had the highest grain yield and agronomic NUE than other treatments due to significant increase in chlorophyl contents, allometric and yield parameters. The highest net benefit was recorded from the cultivar Punjab-2011 fertilized with CAN. In conclusion, use of NP in Punjab-2011 enhanced the grain yield and agronomic NUE. 相似文献
128.
Abstract Extract Among the puzzling aspects of the recently described autosomal recessive polycystic kidney disease (ARPKD) of sheep (Johnstone et al 2005) is the multiplicity of organ systems affected and the phenotypic variations that occur between the various animal species. It has been shown in studies of other inherited polycystic kidney disease syndromes that the normal gene products of a mutant are likely to be involved in ciliary structure and function. The studies that have led to this recognition can be expected to eventually provide the basis for a better understanding of the functioning of this organelle, and the pathogenesis of lesions in the related diseases. It is well known that cilia are important in the perception of light, olfactory stimuli and sound and that motile cilia provide cell motility (e.g. sperm) and transport of mucus and other fluids. Recent research has indicated that the involvement of the primary cili-um/basal body complex is of central importance in the detection and cellular response to extracellular movement of fluid, critical phases of embryonic development, cell cycle regulation and maintenance of cell polarity. Confocal microscopic studies of renal cyst epithelium in ovine ARPKD have shown that only 30% of cells have a cilium and that these are often truncated (McGlashan et al 2005). The observation, although preliminary, strongly supports the argument that the mutant gene in this disease of sheep normally encodes for a protein essential for primary ciliary function. 相似文献
129.
Abstract Extract New Zealand is remarkable for the few species of tick that occur in the country and an apparent absence of tick-borne diseases. There is, however, only a lack of reports of locally-acquired infections which indicates tick-borne spotted fever group (SFG) rickettsias, ehrlichias, anaplasmas and bartonellas do not occur in the country (Roberts et al 2001). To provide more definitive information, we conducted a PCR survey on ticks from New Zealand. 相似文献
130.
Waseem Shahzad Rashid Munir Mohammad Younus Rana Rashid Ahmad Mohammad Sarwar Khan Ghulam Akbar Mohammad Ijaz Fayyaz Mehmood 《Tropical animal health and production》2013,45(3):737-742
Mycoplasma conjunctivae are etiological agents of infectious keratoconjunctivitis (IKC), commonly known as pink-eye in domestic sheep, goats and other wild animals in many parts of the world. A few young Lohi lambs maintained at Livestock Experiment Station (LES), Bahadurnagar, Okara, Pakistan showed clinical signs and symptoms of conjunctivitis, keratitis, severe lacrimation and varying degree of blindness. During January to March, 2011, a total of 36 ocular swabs were collected from IKC affected animals and were processed for isolation, identification, and characterization of M. conjunctivae. Sixteen (44.44 %) out of 36 samples showed turbidity in PPLO broth. Twelve (75 %) out of 16 broth samples showed colony growth on PPLO agar. All 16 (44.44 %) out of 36 turbid broth samples, 12 (75 %) out of 16 cultured on agar plate samples, and 21 (59 %) out of 36 sheep ocular direct swab samples were found positive for M. conjunctivae through polymerase chain reaction test by using M. conjunctivae-specific primer pair McoF1 and McoR1 and detecting a 750 base pair fragment on agarose gel. Topical application of 0.5 % sterile solution of gentamycin (100 mg/ml) (Gentafar 10 %, FARVET, Netherlands) proved suitable for the treatment of IKC in Lohi lambs as all clinical signs of IKC disappeared after 5 days of treatment with this antibiotic. This is the first report about the prevalence, molecular diagnosis, and treatment of M. conjunctivae in Lohi sheep affected with infectious keratoconjunctivitis at LES, Bahadurnagar, Okara, Pakistan. 相似文献