Exploring the silvicultural and economic viability of gap cutting in Mediterranean softwood plantations

Gap cutting consists of the removal of all trees growing on a small surface,varying in size from 200 to 600 m 2.In Italy,gap cutting has been successfully tested for introducing the natural regeneration of native species into softwood plantations,thus accelerating the transition towards climax hardwood stands.Depending on plantation types and conditions,gap cutting should be started at an age between 70 and 80 years and the system should include at least 12 to 15 gaps,in order to produce enough wood to cover costs.Generally,natural regeneration is firmly established within 5-7 years from treatment.Gap cutting also offers superior results to selection thinning,the latter producing about 35% less value and incurring 25% to 30% more costs.As for all regeneration cuts,the success of gap cutting depends on wildlife control in order to prevent extensive damage to the regeneration.     

Assay of the set of all Sudan azodye (I, II, III, IV, and Para-Red) contaminating agents by liquid chromatography-tandem mass spectrometry and isotope dilution methodology

A high-throughput mass spectrometric method is presented for the simultaneous detection of Sudan I, II, III, IV, and Para-Red azodyes in foodstuff. The method is based on the use of deuterium-labeled internal standards and atmospheric pressure chemical ionization (APCI) in a triple-quadrupole instrument. The gas-phase breakdown pattern of each labeled and unlabeled analogue displays the naphthoic moiety as a common fragment. The search for the parents of this common species (parent ion scans) allows, by flow injection and in a single run, the evaluation of the presence of each polluting species spiked in typical foodstuffs. A detailed assay of each azodye was performed by LC-APCI and isotope dilution method, through the multiple reaction monitoring approach, using deuterium-labeled internal standards. Sudan dyes can be quantified above the threshold of 10 ppb except for Sudan Para-Red, for which the limit of quantification was 20 ppb, likely due to the different ionization efficiency.     

Motility of sea urchin Paracentrotus lividus spermatozoa in the post‐activation phase

The characterization of sperm motility patterns, particularly post‐activation changes, is the first step in setting up species‐specific protocols involving gamete management and embryo production, for both aquaculture and laboratory research purposes. This study is aimed at the characterization of the sperm motility pattern of the purple sea urchin Paracentrotus lividus. Semen samples were individually diluted in artificial sea water for sperm motility activation. They were then incubated at 18°C for up to 24 hr. Motility was evaluated on dilution, and 1 hr, 3 hr and 24 hr after activation, by computerized analyser. The semen fertilization capacity was also evaluated. Under our experimental conditions (dilution 1:1,000 in artificial sea water plus 0.05% BSA, 18°C, in the dark), P. lividus semen remained viable for up to 24 hr, as the total motile sperm and the fertilization percentages did not change significantly during the incubation time. In contrast, the mean curvilinear velocity and the subpopulation of rapid sperm (those having a curvilinear velocity > 100 µm/s) slightly but significantly decreased after 3 hr, thereafter remaining unchanged for up to 24 hr after activation. In conclusion, our results show that diluted P. lividus semen can be used for a longer period than that of most fish species, with no need for motility inhibition procedures, supporting its wider use in laboratory research. In addition, the development of artificial fertilization protocols for aquaculture production is simplified by long‐lasting sperm motility.     

Increasing the stocking density in Paracentrotus lividus larviculture: Effects on survival and metamorphosis rates

The development of sustainable methods for sea urchin juvenile production is currently constrained by high mortality rates during larval growth and the high costs of larval rearing systems management. With the aim of developing a method for the production of juveniles of the purple sea urchin Paracentrotus lividus in a medium‐scale recirculating system, the present study focused on the effects of high stocking densities on larval growth. Plutei larvae were reared at three different densities (up to 7 ind/ml) in a semi‐static culture system. Larval survival and metamorphosis success were evaluated in order to identify the most effective density range. The highest metamorphosis rates (80%–95%) were obtained at 4 and 7 larvae/ml. These results are comparable with (and in some cases higher than) those reported for the same species at much lower larval densities. In conclusion, the rearing conditions tested here show for the first time that a significantly higher (4 ind/ml) stocking density than those of traditional P. lividus rearing methods (based on large volumes and low densities) can be adopted, thus supporting the feasibility of an increase in the final output of competent larvae with no increase in rearing volumes.     

A Survey of Logging Contract Rates in the Southern European Alps

A survey of harvesting contracts was conducted in the Alpine regions of France and Italy. The main goal was to produce a benchmark for the harvesting contract rates in the area that may guide forest owners and logging contractors when making their harvesting decisions. The sample included 443 contracts, evenly distributed between France and Italy. The mean tract size was 9.17 ha, while the mean lot size was slightly larger than 500 m³ under bark (ub). Mean removal intensity varied from 70 to 120 m³ ub ha^?1, depending on country and harvesting technique (i.e. ground-based or cable-yarder based). Mean contract rate was 35€ m^?3 ub, but individual contract rates varied significantly between countries and for different harvest techniques. Regression analysis showed that contract rate was strongly affected by tree size, extraction distance and harvest technique. Contrary to expectation, neither tract size nor lot size had any effect on contract rate. This may be explained by the widespread use of mobile operations that are specifically designed for handling small lots and incur minimum relocation cost. The technical factors explored in the study could only explain 40 % of the variability in the dataset, and therefore at least part of the variability must derive from non-technical factors such as local market dynamics and national economics. The study did find significant differences between countries. In particular, ground-based operations were more cost efficient in France, and cable yarder-based operations in Italy.     

Influence of characteristics and extension of a forest road network on the supply cost of forest woodchips

A forest road network in northeastern Italy was explored by field investigations to evaluate the cost of covering annual demand of woodchips according to three scenarios. The first scenario (A) considered the current extent of the forest road network (mainly tractor road) and the associated qualitative characteristics; the second scenario (B) evaluated the possibility of increasing the availability of the technical amount of forest biomass by extending the forest road network by three tractor roads (3.9 km); the third scenario (C) considered the impact on woodchip supply of converting 9.3 km of tractor forest road into truck forest road. At a woodchip price of 60 € t⁻¹, the cost–supply curve of scenario C indicated a woodchip amount of 2886 t year⁻¹, which was 561 t year⁻¹ greater than scenario A and 161 t year⁻¹ greater than scenario B.