犬蝠源呼肠病毒的分离与鉴定

 【目的】从野生蝙蝠体内分离病毒，为开展蝙蝠的病原监测奠定基础。【方法】从广东省韶关采集了30份野生犬蝠样品，研磨后接种Vero E6细胞，从中分离到2株病毒，对其中一株病毒进行研究，使用生物学与分子生物学方法确定其分类学地位。【结果】该毒株在Vero E6细胞上盲传至第4代开始出现细胞病变，表现为细胞内颗粒增多，细胞收缩、变圆，最后细胞从瓶壁脱落。反复冻融3次后，收集细胞及培养液，电镜观察发现，病毒粒子无囊膜，有双层衣壳，呈正二十面体对称，将病毒命名为Bat/China/2003（B/03）。红细胞凝集试验表明该病毒能凝集健康人O型血红细胞，不能凝集SPF鸡、实验用普通级牛、大鼠和豚鼠的红细胞。理化特性试验表明该病毒对氯仿有抵抗力、能耐受pH3.0的酸性环境、50℃水浴1 h病毒丧失感染能力、1 mol•L-1 MgCl2能提高病毒对热的抵抗力。病毒基因组经琼脂糖凝胶电泳分大、中、小3个区段。用哺乳动物呼肠病毒特异性引物进行反转录-聚合酶链式反应（RT-PCR），扩增得到了预期大小的片段。测序结果经NCBI BLAST分析表明，与呼肠病毒科正呼肠病毒属的Ndelle virus核苷酸同源性最高为91.2%。用DNAMAN Multiple Alignment进行序列同源性分析，与哺乳动物呼肠病毒血清1、2、3型的代表株T1L，T2J，T3D的核苷酸同源性分别为89.9%、76.9%、89.9%。【结论】由以上结果推断该病毒为呼肠病毒科的成员。     

Origin软件在研究市售滴灌产品水力性能中的应用

滴灌是一种受到广泛关注的低压节水灌溉技术,滴灌产品作为承栽该技术的载体,其质量直接影响了滴灌技术的推广。通常那些制造偏差小、流量均匀度高、抗堵塞性能强、单位成本低的滴灌产品才能得到更广泛的应用。采用最小二乘法,使用origin软件对市售的4种主要滴灌产品压力一流量关系进行拟合计算,结果表明：使用该软件拟合的流量及水利性...     

野生蔬菜的研究现状

从野生蔬菜的分类、利用价值等方面概述了其研究进展,同时对目前野生蔬菜开发利用中存在的问题进行了论述,并提出相应的解决方法以及下一步的研究方向。     

黄河水量明显减少对下游河流地貌的影响

分析了黄河下游河床和河口区侵蚀及淤积的变化与黄河水沙变化的关系，黄河水量减少，包括总水量和洪峰水量的减少，会引起下游河床淤积，河口地区淤积减弱，甚至侵蚀后退，也会影响河口以外海岸带泥沙的供给情况，造成黄河三角洲海岸的侵蚀后退。     

锦江河斑鳜消化系统特征和食性

对铜仁市锦江河采集的野生斑鳜消化系统和食性进行了研究,并对不同年龄斑鳜消化系统特征和食性进行比较分析。结果表明,口裂长和肠长都随着体长的增长而增大,Ⅲ龄以上成鱼口裂长和肠长的增长速度慢于体长;个体越小的斑鳜,其眼径与头长的比值越大;低龄斑鳜的摄食率和摄食强度明显高于成鱼。     

不同海拔高度对稻米品质性状的影响

对10个杂交稻组合在同一生态区5个不同海拔高度12个品质性状的变异特点进行了研究.结果表明,不同品质性状在不同海拔高度的变异存在很大差异,糙米率、精米率、粒长相对稳定,各组合平均变异系数仅为0.75%,0.60%和3.16%;而垩白度、垩白粒率波动较大,各组合平均变异系数高达92.28%和66.54%.通过聚类分析,可根据稻米品质性状在不同海拔高度的变异系数大小划分为3种类型,第1种是对气候条件变化最敏感的生态敏感型,如垩白度、垩白粒率,第2种是对气候条件变化反应迟钝的生态迟钝型,如糙米率、精米率、粒长,第3种是介于二者之间的中间型.随海拔升高,灌浆结实时间延长,整精米率增大,垩白度、垩白粒率变小,说明高海拔有利于改善稻米品质.     

Research of the Ignition Timing System for Compressed Natural Gas Vehicle

Compared to bi-fuel engine,it uses completely CNG(Compressed nature gas) to replace diesel which can save more oil fuel than bi-fuel engine that reffited diesel engine into CNG engine. A 4102 diesel engine is refitted into CNG engine and a mechanical ignition timing system is designed by the method of gear and belt. The principles and the construction of the system is introduced and the characteristics of the design is analyzed. The system performed well and had good liability when run in a platform. Compared with the previous one, it kept higher dynamic character and emitted lower. It is a cheap one and can be use to reform the diesel engines.     

学为主体教为主导重在创新能力提高

课堂教学是学校教育的中心，也是培养人才的关键一环。培养学生的主体精神是搞好课堂教学的重要手段，发挥教师的主导作用是搞好课堂教学的关键所在，创新能力的培养是提高课堂教学质量的落脚点。     

Analysis of the Lignin Contents and Related Enzymes Activities in Seed Coat Between Black-Seeded and Yellow-Seeded Rapes (Brassica napus L.)

One pair of near isonegic yellow/black seeded rape (Brassica napus L) were used as experimental materials to study the changes of lignin contents and enzymes activities of 4-coumarate: CoA ligase (4CL), Cinnamyl alcohol dehydrogenase (CAD) and ferulate 5-hydroxylase (F5H) in seedcoats during the stage of seed development. The variation analysis showed that the changes of lignin contents and enzyme activities of 4CL, CAD and F5H in seed coat had significant differences between black- and yellow-seeded rapes, and also between different development stages. The correlation analysis demonstrated that the lignin contents were positively correlative to the activities of all three enzymes investigated in the study, and the interactions between them in the seed coat of the two lines. For yellow-seeded rape, the correlation coefficient (0.7262018) of lignin content and the interaction between 4CL and F5H was significant, the lignin contents were highly positively correlative to the activities of CAD and F5H, the interaction between 4CL and CAD, and the interaction between CAD and F5H. For the black-seeded rape, only the lignin content was highly positively correlative to the activity of F5H (the correlation coefficient was 0.772949), the other correlation coefficients, i. e. lignin contents to 4CL, CAD activities, the interactions between the three enzymes were not significant although all the correlation coefficients were above 0.5000. The results suggested that 4CL, CAD and F5H regulated the biosynthesis of lignin in seedcoat of rapes,leading to the lignin contents in the seedcoats of the yellow-seeded rape much lower than that of the black-seeded line,and affecting the thickness of the developing seedcoats in rapes. Therefore, it was likely to change the seedcoat ratio by overexpressing or suppressing the activities of one of the enzymes, both of them or all of them.