植物提取物减少油菜种子带菌量的初步研究

采用冰冻法研究了十种不同植物提取物对油莱种子表面和种子内部真菌生长的影响，发现多种植物提取物能显著抑制油莱种子表面和种子内部真菌的生长，其中韭菜、辣椒和未灭菌大蒜提取液能完全抑制油莱种子表面和种子内部真菌的生长，可初步选为油莱植物型种衣剂中化学农药杀菌剂的替代品。     

干旱胁迫对不同品系紫甘薯光合特性及干物质积累的影响

为了筛选抗旱品系,以14个紫甘薯品种(系)为材料(徐薯28为对照),采用盆栽模拟干旱胁迫的方法,分析评价干旱胁迫对紫甘薯光合特性及干物质积累的影响。干旱胁迫20 d时对其叶片光合特性及干旱胁迫70 d时对其产量进行测定分析。结果表明:干旱胁迫下,参试紫甘薯品种(系)的叶绿素含量指数(CCI)、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、单株薯块鲜质量和干质量表现出不同程度的降低;CCI和Pn值较高的品种为宁紫薯1号和徐紫薯6号;烟薯0747和泰中11号受干旱胁迫影响较大;14个紫甘薯品种(系)中,徐紫薯6号、徐紫薯1号、徐薯33、徐102903、漯紫薯1号、商077-1、宁紫薯1号、徐薯28属气孔限制因素导致的光合速率降低类型,其余品种(系)属于非气孔限制因素导致的光合速率降低类型;研究结果表明:宁紫薯1号、徐紫薯6号抗旱性较强,烟薯0747、泰中11号抗旱性较弱。     

Event-specific detection of seven genetically modified soybean and maizes using multiplex-PCR coupled with oligonucleotide microarray

With the increasing development of genetically modified organism (GMO) detection techniques, the polymerase chain reaction (PCR) technique has been the mainstay for GMO detection. An oligonucleotide microarray is a glass chip to the surface of which an array of oligonucleotides was fixed as spots, each containing numerous copies of a sequence-specific probe that is complementary to a gene of interest. So it is used to detect ten or more targets synchronously. In this research, an event-specific detection strategy based on the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene is being developed for its high specificity using multiplex-PCR together with oligonucleotide microarray. A commercial GM soybean (GTS 40-3-2) and six GM maize events (MON810, MON863, Bt176, Bt11, GA21, and T25) were detected by this method. The results indicate that it is a suitable method for the identification of these GM soybean and maizes.     

A recombinant pseudorabies virus encoding the HA gene from H3N2 subtype swine influenza virus protects mice from virulent challenge

The hemagglutinin (HA) gene of A/Swine/Inner Mogolian/547/2001 (H3N2) swine influenza virus (SIV) was recombined into the genome of pseudorabies virus (PRV) Bartha-K61 vaccine strain, generating a recombinant PRV expressing the HA gene, designated as rPRV-HA. One group of 15 mice was inoculated intranasally (i.n.) with 10(5.0) PFU of rPRV-HA, and another two control groups of mice (15 mice per group) were mock-inoculated or inoculated with Bartha-K61. Mice inoculated with rPRV-HA developed hemagglutination inhibition antibodies 3 weeks post-inoculation. Twenty-eight days post-inoculation, all mice were challenged i.n. with 10(5.0) TCID50 of A/Swine/Heilongjiang/74/2000 (H3N2). No challenge virus was isolated from vaccinated mice, and mild pathological lesions were observed only in lungs following challenge. The results demonstrate that the recombinant rPRV-HA expressing the HA gene from H3N2 SIV can protect mice from heterologous virulent challenge, and may represent a candidate vaccine against SIV.     

杜鹃冠网蝽的药剂防治试验

针对5种杀虫剂进行了杜鹃冠网蝽的田间防治试验,结果表明：20％氯氟·毒死蜱微乳剂、10％烯啶虫胺可溶液剂、25％吡蚜酮可湿性粉剂、5％甲维盐水分散粒剂、24％甲氧虫酰肼悬浮剂是防治杜鹃冠网蝽的有效药剂。氯氟·毒死蜱、烯啶虫胺、吡蚜酮、甲维盐为防治杜鹃冠网蝽的首选药剂,药后第14d的防效均在97％以上。其次是甲氧虫酰肼,药后第14d的防效在80％以上。     

Effect of Leaf Position and Pretreatment on the Regeneration of an Eucalyptus grandis × E.tereticornis Clone

以巨细桉无性系DH201-2叶片为材料进行植株再生研究,揭示叶片位置和切除方式对愈伤组织诱导和植株再生的影响,结果表明:叶片位置对叶片培养死亡率与叶片植株再生率、叶片植株再生芽数影响显著,对愈伤产生率基本没影响.以组培生根苗第1～6片叶作外植体进行愈伤培养与植株再生培养效果较好,以第1～2片叶作外植体效果最佳,第7～8片叶不宜作为外植体培养;拉下或剪下全叶两种方式,植株再生率与再生芽数较高,全叶剪除1/3叶尖方式效果最佳,能有效加速叶片的愈伤生长及植株分化,提高叶片植株再生率及再生芽数.全叶剪除叶柄及1/3叶尖方式效果最差,植株再生率、再生芽数效果最低.培养50d,愈伤植株再生率及再生芽数达到高峰期.     

Effect of microtubule-destabilizing protein stathmin gene silencing on proliferation and apoptosis of nasopharyngeal carcinoma cell line 5-8F

AIM: To investigate the effects of stathmin gene silencing on nasopharyngeal carcinoma cell line 5-8F. METHODS: Double-strand siRNA targeting to stathmin gene was obtained by chemical synthesis and annealing, and was sub-cloned into the vector pGenesil-1.1. The plasmid was introduced into 5-8F cells by liposome-mediated transfection. The gene expression of stathmin, and the proliferation, morphology and apoptosis of the cells were analyzed by Western blotting, MTT assay and flow cytometry. RESULTS: The cell suppression rate in stathmin gene silencing group was (53.01?1.12)%, significantly higher than that in transfection reagent group and in negative control group. The cell apoptotic rate in stathmin gene silencing group was (8.75?0.67)%, also significantly higher than that in transfection reagent group and in negative control group (P<0.05). CONCLUSION: Silencing of stathmin gene in nasopharyngeal carcinoma cells inhibits the cell proliferation and induces cell apoptosis.     

四种不同香型乌龙茶在卷烟滤嘴中的应用研究

以铁观音清香型茶、铁观音浓香型茶、黄金桂清香型茶、黄金桂浓香型茶4种具有代表性的不同香型安溪乌龙茶为研究对象,采用CO2超临界萃取法将其进行提取,得到提取物。以固相微萃取-顶空进样,GC-MS法对不同香型安溪乌龙茶提取物进行分析,比较各香型提取物的挥发性成分和致香成分的差异。同时将其应用于卷烟丝束当中,评价卷烟抽吸品质,探讨其在卷烟滤棒中应用时的增香效果。结果表明,黄金桂清香型茶超临界萃取物中致香成分最为丰富,特征成分含量较高,同时与卷烟烟气的谐调性较好,能显著改善卷烟的抽吸品质,具有较好的开发应用前景。     

红黄壤茶园不同培肥模式的土壤理化效应

通过长期定位试验,研究闽东地区红黄壤茶园不施肥(CK)、全量化肥(C)、(半量化肥+半量有机肥)(C1/2O1/2)、全量有机肥(O)、(全量化肥+豆科绿肥)(CL)及(半量化肥+半量有机肥+豆科绿肥)(C1/2O1/2L)等6种不同培肥模式下土壤理化性质的动态变化。结果表明:与CK模式相比,其他模式均能一定程度上改良土壤理化性状。其中,处理O和C1/2O1/2L的改良效益相对较佳,砂粒(0.05 mm相似文献