Electroacupuncture-induced neural activation detected by use of manganese-enhanced functional magnetic resonance imaging in rabbits

OBJECTIVE: To investigate the effects of acupuncture on neural activity detected by use of manganese-enhanced functional magnetic resonance imaging (fMRI) and elucidate the relationship between somatic acupoint stimulation and brain activation. ANIMALS: 40 New Zealand White rabbits. PROCEDURE: Manganese-enhanced fMRI was performed in anesthetized rabbits manipulated with electroacupuncture (EA) on Zusanli (ST-36) and Yanglingquan (GB-34) acupoints. Image acquisition was performed on a 1.5T superconductive clinical scanner with a circular polarized extremity coil. T1-weighted images were acquired sequentially as follows: baseline, after mannitol injection, after manganese infusion, and 5 and 20 minutes after initiation of EA. RESULTS: Changes in focal neural activity were detected by use of manganese-enhanced fMRI. Stimulation on Zusanli (ST-36) for 5 minutes resulted in activation of the hippocampus, whereas stimulation on Yanglingquan (GB-34) resulted in activation of the hypothalamus, insula, and motor cortex. Activation became less specific after 20 minutes of EA. Furthermore, stimulation on ipsilateral acupoints led to bilateral brain activation. CONCLUSIONS AND CLINICAL RELEVANCE: Each acupoint has a corresponding cerebral linkage, and stimulation on these points resulted in time-dependent neural activation. Understanding the linkage between peripheral acupoint stimulation and central neural pathways may provide a useful guide for clinical applications of acupuncture.     

“赛豹”追踪侦破女尸案

2010年以来,云南红河州个旧市一些不法分子大肆对锡矿石进行盗抢,给国家造成重大经济损失。为此,个旧市委、市政府决定对矿山进行一次专项整治,制止大规模集体盗抢违法行为的发生,个旧市公安局于3月12日抽调了4头警犬进驻治安较为复杂的1800矿区参与治安专项整治,掀开了矿山整治的序幕。目前,1800矿区的盗抢案件大幅度下降,警犬在专项整治中发挥了重要作用,收到了良好成效。     

Effects of supplemental vitamin E during the mature period on the reproduction performance of Taiwan Native Chicken cockerels

One-day-old Taiwan native male chicks were fed with maize-soybean rearing diets without supplemental vitamin E to 23 weeks of age. From 23 to 52 weeks of age, the cockerels (n = 90) were assigned at random to 5 dietary treatments and fed with maize-soybean diets supplemented with 0, 20, 40, 80 and 160 mg/kg of vitamin E (dl-alpha-tocopherol acetate). Pullets (225) of the same age were fed with standard diets throughout. They were artificially inseminated with one dose of 0.04 ml/bird intact and 5-fold diluted pooled semen at 31 to 43 weeks of age and at 49 weeks of age, respectively. The criteria evaluated included: semen quality, fertility and maximum and effective duration of fertility, blood characteristics, body and testes weight. Supplemental vitamin E did not affect cockerels' effective duration of fertility and percentage of fertility. However, when pullets were inseminated with diluted semen, supplementing 160 mg/kg vitamin E increased the maximum duration of fertility at 49 weeks of age. Cockerels receiving 40 to 160mg/kg supplements had higher sperm viability and motility after 39 weeks of age and those fed 80 mg/kg had higher sperm concentration at 39 weeks of age. Cockerels receiving supplements of more than 40 mg/kg vitamin E had higher body weight gain. Plasma cholesterol and testosterone were not affected by supplemental vitamin E. However, plasma luteinising hormone (LH) concentration was lower in cockerels fed 160 mg/kg. Lack of supplemental vitamin E over 39 weeks was associated with lower semen quality but did not reduce the proportion of fertile eggs laid by inseminated hens, perhaps because the insemination dose compensated for low sperm quality. We found that the maximum duration of fertility might be improved by supplementing 160 mg/kg vitamin E at 49 weeks of age.     

高效液相色谱法测定猪血浆及组织中喹赛多及其脱二氧代谢物

建立了测定猪血浆及肝脏、肾脏、肌肉等组织中的喹赛多及其代谢物脱二氧喹赛多的高效液相色谱(HPLC)法.血浆样用甲醇沉淀蛋白后离心取上清液进样测定.组织样先用乙腈匀浆,用正己烷脱脂后作HPLC检测.色谱柱为ODS C18柱;流动相血浆样测定为乙腈水(2080),组织样测定为甲醇水(4258),流速为1.0 mL/min;紫外检测波长305 nm.药物工作液质量浓度范围为0.005～0.500 mg/L时,血浆样品及组织样品测定条件下药物质量浓度与响应值均具良好线性关系,相关系数＞0.999.血浆中药物质量浓度为0.02、0.10、0.50 mg/L时,喹赛多及脱二氧喹赛多的回收率均大于70%,组织中药物含量为0.05、0.20、1.00μg/g时,肌肉样品的回收率均大于70%,肝脏、肾脏样品则为50%～80%.本试验条件下,喹赛多及其脱二氧代谢物的最低检出质量浓度,血浆样品分别为0.01、0.02mg/L,组织样品2种检测物均为0.025μg/g.测定了工作液3种质量浓度0.01、0.05、0.25 mg/L的仪器精密度,日内相对偏差＜8.0%,日间相对偏差＜17.0%.     

饲料原料与瘤胃培养残渣(8、12、16小时)氨基酸组成差异的研究

选用3头装有瘤胃瘘管的改良阉牛 ,利用尼龙袋技术对不同饲料降解后残渣的氨基酸(AA)组成与原料AA组成的差异进行了研究。结果表明 ,与精料氨基酸浓度相比在降解后有上升趋势(P<0.05)的氨基酸有Ala、Phe、Val、Ile和Leu(其中后3种氨基酸为支链氨基酸) ;在降解后有下降趋势(P<0.05)的氨基酸有Gly、Arg、Tys、Lys、Met和Cys。各氨基酸降解前后的浓度存在强相关回归关系。     

饲喂不同比例小叶章日粮对奶牛瘤胃干物质降解率的研究

本文采用拉丁方实验设计,利用瘤胃尼龙袋技术,测定适宜期收获的小叶章牧草占粗饲料不同比例时其干物质(DM)在奶牛瘤胃中的降解率。结果表明：饲喂不同比例小叶章牧草时DM在奶牛瘤胃降解率依次为42．3％、46．7％、44．5%。测试结果证明,当小叶章牧草占日粮粗饲料25％比例时其对奶牛的饲用价值较高。     

紫花苜蓿灌溉的理论与技术

本文系统地阐述了紫花苜蓿灌溉的理论与技术,内容包括需水量、需水强度、入渗速率、灌溉需要量、灌溉定额、灌水深度、灌水定额、灌水周期、灌水次数、灌水时期、灌水强度、灌溉方法等内容。     

吉林省主要粮食作物比较优势分析

根据比较优势理论，应用综合比较优势指数法对吉林省玉米、水稻、大豆三种主要粮食作物在1997-2005年间的比较优势变动趋势进行了分析，其结果表明：三种作物中，玉米具有明显的比较优势，大豆次之，水稻则处于劣势，但呈现出稳步上升的趋势；并在此基础上提出了吉林省主要粮食作物结构调整的建议。     

对《我县首例动物防疫行政复议案件顺利结案》一文的再商榷

《中国动物检疫》2011年第1期,刊登了江苏省如东县动物卫生监督所高丰撰写的《我县首例动物防疫行政复议案件顺利结案》一文,本刊第4期又刊登了陕西省汉中市汉台区动物卫生监督所任润和等作者撰写的《对〈我县首例动物防疫行政复议案件顺利结案〉一文的商榷》,本作者与前两期作者在法律适用     

如何选择缉毒犬

实践已证明,在犬群中只有约二十分之一的犬适宜作为缉毒犬,因为本身的自然素质及心理素质存在着一定的差异,犬的潜能也是有区别的.好的受训犬能节省训犬员训练时间,提高训练成功率及训犬员的信心,更好地为人民服务.