Relationship between changes of soil microbial biomass content and imazamox and benfluralin degradation

The present research was carried out to determine the relationship between the soil microbial biomass content and the persistence of imazamox and benfluralin in three different soils, incubated in the laboratory under different conditions. The half-life values varied between 17.1 and 92.4 days for imazamox and 11.4 and 37.9 days for benfluralin depending on initial concentration, temperature, soil moisture and soil type. Significant decreases in microbial biomass-C content compared to untreated soil were observed initially, not exceeding 25.0% for imazamox and reaching 64.7% for benfluralin. The microbial biomass-C content then returned to initial values at varying times depending on incubation conditions. The relationship between herbicide persistence and microbial biomass-C content gave parabolic curves (P<0.001 in all cases) under all conditions tested. At the time of maximum microbial biomass decrease, the concentration of imazamox was generally about 50% of the initial dose (except for at 10°C for imazamox, when the biomass began to recover as early as the point when the pesticide concentration was at 60-65% of its initial dose). The final equation proposed could be useful to deduce the decrease in soil microbial biomass in relation to the herbicide level.     

Response to water stress of some oleander cultivars suitable for pot plant production

Oleander (Nerium oleander L.) is an evergreen shrub of great ornamental interest which, in recent times, has been increasingly used as a flowering pot plant. Plants grown in pots undergo more frequent water stress conditions than those grown in the soil, due to the limited volume of substrate available for the roots. Oleander is a species adaptable to dry conditions and able to survive long periods of drought. It is well known that under water stress conditions all plants reduce photosynthetic activity, resulting in reduced plant growth. In case of severe water stress, leaves undergo strong wilting and senescence resulting in the loss of ornamental value. In the present work, a study was conducted to evaluate the ecophysiological response to water stress in four oleander cultivars previously recognised (on the basis of traits such as size, habit, earliness, abundance and duration of flowering, aptitude for cutting propagation and rapidity of growth) as suitable for pot plant production. Our data confirm the high drought tolerance of oleander. In the studied cultivars, plants submitted to water stress showed only minor variations in leaf gas exchange parameters [transpiration (E), stomatal conductance (g_s) and CO₂ net assimilation (A)] for at least 10 days without a change in leaf water content [assessed as relative water content (RWC)] for 22 days from the beginning of the stress treatment. During this period, non-irrigated plants maintained the same water status as control plants and were visually undistinguishable from them. Moreover, plants survived without water supply for one month. Following the supply of water again, they were able to restore RWC, gas exchange parameters and instantaneous water use efficiency [A/E ratio (WUE_inst)] to the values of control plants. Furthermore, if at the end of the stress period plants appeared withered and were pale green in colour, they regained their normal appearance after they were irrigated again. Although the four studied cultivars showed some minor differences in leaf gas exchange parameters and in the manner in which the latter parameters changed after irrigation was stopped, the response to water stress was essentially the same. Therefore, as far as drought tolerance is concerned, all these cultivars have a good aptitude for use as flowering pot plants.     

Estimation of basket trap selectivity for changeable nassa (Nassarius mutabilis) in the Adriatic Sea

Size selectivity of basket traps for changeable nassa (Nassarius mutabilis) was estimated through comparative fishing trials with traps varying in mesh size and net colour. Selectivity curves of two commercial (19 mm white twine and 19 mm black twine) and three experimental (23, 26 and 28 mm white twine) traps were estimated using SELECT method. In addition, theoretical size-selectivity curves were calculated from morphometric data using a simplified version of Sechin method.Differing from the opinion of fishermen, N. mutabilis did not show any preference for dark coloured twine. Retention curves estimated with SELECT method had a very sharp selectivity and well fitted catch data in all cases. Theoretical size-selectivity curves were very close to size-selectivity curves obtained from experimental data. The two types of curve were very similar in shape, although the theoretical ones were shifted towards larger size classes and showed a slightly steeper slope than SELECT curves. The mesh size commonly used by fishermen is not adequate for rationally exploiting the resource, as catches mainly included undersized specimens, whilst the 23 and 26 mm mesh sizes would represent a good compromise between reduced occurrence of undersized individuals and satisfactory yields of commercial product.     

Do water dynamics and land use in riparian areas change the spatial pattern of physical–mechanical properties of a Cambisol?

Precision Agriculture - Changes in land use in riverside ecosystems added to the flood and ebb regimes of rivers modify the spatial variability of the physical–mechanical attributes of soil,...     

Rhabdomyosarcoma in 8 horses

This multi-institutional report describes 8 cases of rhabdomyosarcoma in horses. Four neoplasms were in the tongue and other areas of the mouth or head, 2 were in the abdominal wall, and 1 each was in right shoulder muscles and heart. Four rhabdomyosarcomas that were less than 10 cm in diameter were treated by surgical excision or radiation with no recurrence. Two neoplasms greater than 10 cm in diameter in the abdominal wall and the right shoulder were considered inoperable and led to decisions to euthanize the horses. Two neoplasms were incidental findings at necropsy. All the neoplasms were classified as embryonal except for 1 pleomorphic rhabdomyosarcoma. These 8 cases were evaluated with 9 published case reports of equine rhabdomyosarcoma. For all cases, the most common sites were limb muscles (5/17) and tongue (4/17). Metastasis was reported in 4 of the previously published cases; none was found in this study.     

Amplified fragment length polymorphism for variety identification and genetic diversity assessment in oleander (Nerium oleander L.)

Oleander is a Mediterranean evergreen shrub found along watercourses, gravelly places and damp slopes. It is grown widely as an ornamental for its abundant and long-lasting flowering as well as its moderate hardiness. Genetic relatedness among 71 accessions, including commercial varieties, different sources of the same varieties, and selections from the wild were investigated using amplified fragment length polymorphism (AFLP). Nine primer combinations yielded a total of 603 bands of which 241 were polymorphic. Genetic similarities among accessions were calculated according to Jaccard's Similarity Index and used to construct a dendrogram based on the unweighted pair group method using arithmetic averages. Our results show that the AFLP technique, which can simultaneously and assay a large number of loci randomly distributed in the genome, is much more informative on the genetic relationship and origin of accessions than the limited number of morphological characters conventionally used for variety discrimination. Up to about 9% molecular genetic differentiation was detected among morphologically indistinguishable provenances of the same variety; this can be partly attributed to scoring error but mainly to somatic variation occurring during vegetative propagation. On the other hand lower genetic distance values were sometimes found among varieties which differ in morphological characters and are thus commercialised with different names. The possibility of considering the amount of genetic variation within a variety as the threshold value for discrimination of initial varieties and essentially derived varieties is discussed. This revised version was published online in July 2006 with corrections to the Cover Date.     

Cutaneous neosporosis in two adult dogs on chronic immunosuppressive therapy.

Antemortem diagnosis of generalized ulcerative and pyogranulomatous dermatitis with numerous intralesional tachyzoites was made from skin biopsy specimens from 2 adult dogs on chronic immunosuppressive therapy. A 9-year-old Italian Greyhound was on long-term corticosteroid therapy for the treatment of a lupus-like systemic autoimmune disorder, and a 7-year-old Labrador Retriever had received several months of chemotherapy for lymphosarcoma. The tachyzoites were identified as Neospora caninum by immunoperoxidase immunohistochemistry. Both dogs were treated with clindamycin. Lesions in the Greyhound resolved; however, the Labrador Retriever was euthanized because of evidence of neuromuscular disease, despite improvement of the skin lesions. These 2 cases indicate that cutaneous neosporosis can occur in adult dogs on chronic immunosuppressive therapy. The disease may result from reactivation of a congenital infection and/or a recently acquired primary infection.     

Analysis of insecticide-resistant Myzus persicae (Sulzer) populations collected in Italian peach orchards

Several populations of the green peach aphid, Myzus persicae (Sulzer), were collected in the most important Italian peach-growing areas. In these populations, collected as primary colonies or as secondary ones, total esterase content and acetylcholinesterase sensitivity towards pirimicarb was assessed using biochemical assays. Most of the individuals analysed generally exhibited an elevated esterase content, and in some cases acetylcholinesterase insensitivity. The implications of resistance levels and distribution on M persicae control strategies are discussed.