The triple-ring nebula around SN 1987A: fingerprint of a binary merger

Supernova 1987A, the first naked-eye supernova observed since Kepler's supernova in 1604, defies a number of theoretical expectations. Its anomalies have long been attributed to a merger between two massive stars that occurred some 20,000 years before the explosion, but so far there has been no conclusive proof that this merger took place. Here, we present three-dimensional hydrodynamical simulations of the mass ejection associated with such a merger and the subsequent evolution of the ejecta, and we show that this accurately reproduces the properties of the triple-ring nebula surrounding the supernova.     

Stathmin-tubulin interaction gradients in motile and mitotic cells

The spatial organization of the microtubule cytoskeleton is thought to be directed by steady-state activity gradients of diffusible regulatory molecules. We visualized such intracellular gradients by monitoring the interaction between tubulin and a regulator of microtubule dynamics, stathmin, using a fluorescence resonance energy transfer (FRET) biosensor. These gradients were observed both during interphase in motile membrane protrusions and during mitosis around chromosomes, which suggests that a similar mechanism may contribute to the creation of polarized microtubule structures. These interaction patterns are likely to reflect phosphorylation of stathmin in these areas.     

Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions

Certain inflammatory stimuli render cultured human vascular endothelial cells hyperadhesive for neutrophils. This state is transient and reversible, in part because activated endothelial cells secrete a leukocyte adhesion inhibitor (LAI). LAI was identified as endothelial interleukin-8 (IL-8), the predominant species of which is an extended amino-terminal IL-8 variant. At nanomolar concentrations, purified endothelial IL-8 and recombinant human IL-8 inhibit neutrophil adhesion to cytokine-activated endothelial monolayers and protect these monolayers from neutrophil-mediated damage. These findings suggest that endothelial-derived IL-8 may function to attenuate inflammatory events at the interface between vessel wall and blood.     

An integrated genomic analysis of human glioblastoma multiforme

Glioblastoma multiforme (GBM) is the most common and lethal type of brain cancer. To identify the genetic alterations in GBMs, we sequenced 20,661 protein coding genes, determined the presence of amplifications and deletions using high-density oligonucleotide arrays, and performed gene expression analyses using next-generation sequencing technologies in 22 human tumor samples. This comprehensive analysis led to the discovery of a variety of genes that were not known to be altered in GBMs. Most notably, we found recurrent mutations in the active site of isocitrate dehydrogenase 1 (IDH1) in 12% of GBM patients. Mutations in IDH1 occurred in a large fraction of young patients and in most patients with secondary GBMs and were associated with an increase in overall survival. These studies demonstrate the value of unbiased genomic analyses in the characterization of human brain cancer and identify a potentially useful genetic alteration for the classification and targeted therapy of GBMs.     

Hobbyists acting simultaneously as anglers and aquarists: Novel pathways for non-native fish and impacts on native fish

1. Human translocations of non-native aquatic species are a global conservation challenge. Aquarists and anglers are two hobbyist groups whose practices are particularly likely to translocate non-native aquatic species, especially fish.
2. This article aims to stipulate a change of perspective among these hobbyists, who – by acting simultaneously as anglers and aquarists – establish novel pathways for non-native fish.
3. This study showed that: (i) 51 of 226 respondents to a questionnaire act simultaneously as anglers and aquarists; (ii) 53 of 237 non-native fish species in the European Union are used for both angling and aquarium hobbies; and (iii) hobbyists write online reports on the catch and exchange of a particularly invasive fish, the round goby (Neogobius melanostomus).
4. This combination of knowledge on how hobbyists use non-native fish and the ecological impacts of round goby demonstrates how the impact of an invasive species can be influenced by the combined behaviours of anglers and aquarists.
5. We suggest that future research in aquatic conservation should move beyond considering the commercial aquarium trade and angling as separate pathways for translocating non-native fish, and instead consider these hobbyist groups as interconnected.

     

Predation on native fish eggs by invasive round goby revealed by species‐specific gut content DNA analyses

1. Conservation of riverine fish often aims to improve access to spawning grounds and restore longitudinal connectivity by removing migration barriers, and involves substantial investments. However, these investments also enable non‐native predators to invade upstream into spawning areas and potentially adversely affect the recruitment of threatened freshwater fish through egg or fry predation.
2. Detecting egg predation is often challenging. Visual inspections of fish gut contents may underestimate predation of soft materials such as eggs and fry, which limits the discovery of predators preying upon these life‐stages. DNA‐based detection assays may offer a more sensitive tool to assess predation of soft materials.
3. A conservation issue was confirmed by developing and applying a species‐specific DNA‐based detection assay: invasive round goby (Neogobius melanostomus) prey on the eggs or fry of the threatened common nase (Chondrostoma nasus) in Switzerland.
4. DNA‐based detection assays were also developed for five other valuable native fish species, including endangered salmonid and cyprinid river spawners. The applicability of the assays was confirmed in a series of laboratory and field feeding experiments involving eggs and fish tissue. In addition, this work provides a guiding framework for conservation managers regarding the use and applicability of different DNA‐based detection approaches for gut content analysis.
5. The results of this study could inform local conservation measures – such as temporary reductions in the density of round goby at spawning sites prior to spawning – and demonstrate how targeted application of species‐specific molecular markers may advance freshwater fish management.

     

Viscoelasticity and shear resistance at the aggregate scale of structured and organic carbon-free Chernozems

Soil rheology characterises the flow behaviour of soils at the particle-particle to aggregate scale. Amplitude sweep tests (ASTs) are often the method of choice for parameterizing soil flow properties, such as the shear strain values at the end of the linear viscoelastic range (i.e., the deformation is mainly elastic) and at the yield point (i.e., elastic equals plastic deformation). Samples from seven soil profiles and five soil depths of Chernozems, collected in the Maidanetske study area, clo...     

Competitive sorption of linear alkylbenzene sulfonate (LAS) surfactants and the antibiotics sulfamethoxazole and ciprofloxacin in wastewater-irrigated soils of the Mezquital Valley,Mexico

Purpose

The increasing reuse of wastewater for irrigation introduces surfactants and antibiotics into the environment. How these two kinds of compounds interact with regard to their sorption processes in soil is not clear.

Materials and methods

We performed batch experiments to investigate the sorption of linear alkylbenzene sulfonates (LAS) and its effect on sorption of sulfamethoxazole and ciprofloxacin in irrigated and non-irrigated soils with different organic matter (OM) contents.

Results and discussion

LAS sorption was non-linear in the presence of the antibiotics, and as general trend, it increased with rising OM content of soils. Free LAS was also removed from solution by complexation with Ca²⁺. Dissolved organic compounds released from soils with OM contents ≥18.4 g kg^?1 further reduced LAS sorption. Sorption of sulfamethoxazole was reduced by LAS sorption only in one soil with a small OM content of 9.5 g kg^?1.

Conclusions

The strong sorption of ciprofloxacin is not affected by LAS. Sulfamethoxazole sorption only competes with LAS sorption in organic matter-poor soils. Accumulation of organic matter in soils, for example due to long-term wastewater irrigation, provides extra sorption capacity for LAS and sulfamethoxazole so that competition for sorption sites is reduced.