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851.
Aphid-borne viruses are responsible for major cucurbit diseases and hamper the sustainability of crop production. Systematic monitoring can reveal the occurrence and distribution of these viruses, in addition to unadvertised viruses, facilitating the control of diseases. For three consecutive (2018–2020) seasons, the presence of aphid-borne viruses was monitored from a total of 292 samples of watermelon and squash plants that showed yellowing symptoms in three major cucurbit-producing areas (Castilla La-Mancha, Alicante, and Murcia) in Spain. We observed that cucurbit aphid-borne yellows virus (CABYV) was the most common virus found (29%) in the plants from both crops. Likewise, except for squash samples from Castilla La-Mancha and Alicante, watermelon mosaic virus (WMV) was also found (23%) with a relatively high frequency. Furthermore, we observed the exacerbation of bright yellowing symptoms in watermelon plants that was often accompanied by considerable fruit abortion. CABYV was the only causative agent for this new yellowing disease, and two infectious cDNA clones (one from watermelon, CABYV-LP63, and another from melon, CABYV-MEC12.1) were constructed to further compare and characterize this CABYV disease. Based on the full-length genome, both isolates were grouped phylogenetically together within the Mediterranean clade. However, the Koch's postulates tests were only successfully completed for the LP63 isolate, which also showed several amino acid changes and two potential recombination events, as compared to MEC12.1. Remarkably, the LP63 isolate caused more severe symptoms and showed higher RNA accumulation than MEC12.1 in five cucurbit plant species. These results suggest that a novel CABYV variant that causes severe yellowing symptoms may be causing outbreaks in cucurbit crops. 相似文献
852.
Liu Shaoshuai Ladera-Carmona Maria Jose Poranen Minna M. van Bel Aart J. E. Kogel Karl-Heinz Imani Jafargholi 《植物病害和植物保护杂志》2021,128(5):1201-1212
Journal of Plant Diseases and Protection - Macrophage migration inhibitory factors (MIFs) are multifunctional proteins regulating major processes in mammals, including activation of innate immune... 相似文献
853.
854.
Adriana Príncipe Alice Nunes Pedro Pinho Lúcio do Rosário Otília Correia Cristina Branquinho 《European Journal of Forest Research》2014,133(4):757-767
Understanding forest regeneration at sites previously used for agriculture underlies the establishment of science-based woodlands management policies. This is especially relevant in semi-arid areas, where the tree cover is critical in ameliorating the effects of aridity and in preventing desertification and land degradation. Natural regeneration in semi-arid areas occurs very slowly, which in part explains why it has hardly been studied. In the present work, we sought to devise a method to predict the natural regeneration potential of woodlands in semi-arid areas, to be used in guiding restoration efforts. Specifically, we evaluated holm oak coverage at a long-term ecological research site and then designed and validated a model to predict the natural regeneration of holm oak based on a few environmental variables. Unlike available studies, we obtained long-term information on tree regeneration (making use of >60 years of aerial photography) and climate (using long-term climate and microclimate data). We found that microclimate, measured using the potential solar radiation as a proxy, was a key driver of natural regeneration: after 60 years of agricultural abandonment, less sun-exposed areas attained a tree cover >90 %, whereas in more sun-exposed areas it remained below 20 %. We then used the model to map the natural regeneration potential, first in the study area and then in an area where holm oak plantations had been unsuccessfully introduced. In the latter case, the model successfully predicted the failure of this reforestation effort. Our results support the use of this model by decision makers to optimize management practices, as it will encourage the concentration of efforts in areas more prone to successful reforestation and allow the identification of areas more likely to benefit from natural regeneration processes. 相似文献
855.
Magnus Fredriksson Olof Broman Fredrik Persson Ann Axelsson Pedro Ah Shenga 《Wood material science & engineering》2014,9(1):31-39
With the development of scanning technology in sawmills, it is possible to optimise log rotational position when sawing. However, choosing a different rotational position than horns down might be detrimental for the board shape after drying, especially for curved logs. Thus, there is a need to investigate at what level of log curve it is possible to freely rotate logs without causing board warp. This study was carried out through a test sawing that was conducted at a sawmill situated in the middle of Sweden. The tests were made on 177 Norway spruce logs, with varying amount of curve. Half of the logs were sawn in the horns-down position, half were sawn rotated perpendicular to horns down. Log shape and warp of the dried boards were measured. The results indicated a relationship between board spring, log curve and choice of rotational position. Furthermore, board bow was related to log curve but not rotational position. It can be concluded that for straight logs, with a bow height of less than 15 mm, an unconventional rotational position does not cause excess spring in the boards. Bow and twist are not affected by the rotational position at all. 相似文献
856.
Julian A. Bartolome Pablo Khalloub Rodolfo Luzbel de la Sota Marc Drillich Pedro G. Melendez 《Tropical animal health and production》2014,46(1):79-85
The objectives of this study were to evaluate the efficacy of (1) administering ceftiofur hydrochloride in dairy cows with calving-related disorders to prevent metritis and (2) a combination of GnRH and PGF2α for the treatment of clinical endometritis, under Argentinean dairy farming conditions. Cows at high risk (HRC) for metritis (dystocia, RFM >12 h postpartum, hypocalcaemia, twins, or stillbirth) were randomly assigned to receive either 1.1 mg/Kg of ceftiofur hydrochloride on three consecutive days (HRC treated group HRCT, n?=?110) or remained untreated (HRC control group HRCC, n?=?126). Cows with low risk (LRC, no calving-related disorders, n?=?868) did not receive any treatment (LRC group, n?=?868). All cows were examined for metritis between days 4 and 10 and for clinical endometritis between 24 and 30 days postpartum. The body condition score (BCS) was recorded at both examinations. Cows with endometritis at days 24 to 30 postpartum received either 1.5 mg of D-cloprostenol (PGF; n?=?129) or 100 μg of GnRH followed by D-cloprostenol after 7 days (GnRH+PGF, n?=?119). There was no overall effect of treatment on the incidence of metritis or on time to pregnancy. Treatment, however, reduced the incidence of metritis in cows with high BCS (HRCT?=?24.0 %, HRCC?=?38.5 %) but had no effect in cows with low BCS (HRCT?=?38.7 %, HRCC?=?37.5 %). The proportion of pregnant cows by days in milk was greater (P?<?0.01) in LRC group compared with that of the HRCT and HRCC groups. No significant differences were found between groups PG and PG+GNRH. GnRH+PGF treatment, however, tended (P?=?0.06) to increase pregnancy rate in cows with a moderate loss of BCS (76.5 vs 65.2 %) but tended to reduce pregnancy rate (54.5 vs 76.0 %) in cows with a more pronounced loss in BCS (>0.75 points). 相似文献
857.
858.
Pablo J. Pinedo Claus D. Buergelt G. Art Donovan Pedro Melendez Laurence Morel Rongling Wu Taimour Y. Langaee D. Owen Rae 《Preventive veterinary medicine》2009,91(2-4):189-196
Paratuberculosis (Johne's disease) imposes a significant problem to the world dairy and beef industries and today is considered a potential zoonosis. The disease is caused by Mycobacterium avium subsp. paratuberculosis and is characterized by progressive weight loss and profuse diarrhoea. Susceptibility to infection is suspected to have a genetic component, and moderated values for heritability of infection have been reported. Interferon gamma is an inducible cytokine with a crucial role in the innate host response to intracellular bacteria. Toll-like receptors are trans-membrane structures responsible for coordination of innate and adaptive immune responses. The solute carrier family 11 member 1 (SLC11A1, formerly NRAMP1) gene plays an important role in innate immunity, preventing bacterial growth in macrophages during the initial stages of infection. The objective of this candidate gene case–control study was to characterize the distribution of polymorphisms in three candidate genes related to the immune function; interferon gamma (BoIFNG), toll-like receptor 4 (TLR4), and SLC11A1 genes and to test their role as potential risk factors for paratuberculosis infection in cattle. The statistical analysis demonstrated significant differences in allelic frequencies between cases and controls for BoIFNG-SNP12781 and SLC11A1 microsatellites, indicating a significant association between infection and variant alleles. In the analysis of genotypes, a significant association was also found between infection status and BoIFNG-SNP12781 and SLC11A1-275-279-281 microsatellites. However, when variables such as breed and age were included in the multivariate logistic regression analysis, a tendency toward statistical significance for the effect of polymorphisms in the odds of infection was only found for alleles SLC11A1-275 and 279. 相似文献
859.
Carina García-Feliz Ana Carvajal Jesús ngel Collazos Pedro Rubio 《Preventive veterinary medicine》2009,91(2-4):130-136
Herd-level risk factors for faecal shedding of Salmonella enterica were investigated in a cross-sectional study on Spanish finishing units. For this purpose, 10 faecal samples were collected from 10 different pens containing pigs close to market weight in a total of 232 fattening units. The total sample size was proportionally distributed according to the fattener census in each of the regions and provinces of the country in order to ensure a sample representative of the entire swine population. All samples were individually examined by culture of 25 g of faecal material. Data regarding characteristics and management of each fattening unit were collected by means of a questionnaire. Logistic regression was used to detect relationships between the detection of faecal shedding of S. enterica and potential herd-level risk factors. The feeding of pelleted feed was associated with an increased risk of culture-positive faecal samples (OR = 2.28; 95% CI: 1.22, 4.26). The odds of a farm being Salmonella positive were associated with its size. Fattening units that slaughtered more than 3500 pigs per year had a higher risk for Salmonella faecal shedding (OR = 1.78; 95% CI: 0.96, 3.31). Interventions at these two points should be considered when designing or managing growing pig facilities to reduce Salmonella faecal shedding by fatteners. 相似文献
860.
Ruth Müller Cornelia Bandow Anne Seeland Dennis Fennel Anja Coors Peter K. Ebke Bernhard Förster Pedro Martinez-Arbizu Thomas Moser Matthias Oetken Jasmin Renz Jörg Römbke Nadine Schulz Jörg Oehlmann 《Journal of Soils and Sediments》2010,10(4):718-721