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11.
Louis R. Iverson Frank R. ThompsonIII Stephen Matthews Matthew Peters Anantha Prasad William D. Dijak Jacob Fraser Wen J. Wang Brice Hanberry Hong He Maria Janowiak Patricia Butler Leslie Brandt Christopher Swanston 《Landscape Ecology》2017,32(7):1327-1346
Context
Species distribution models (SDM) establish statistical relationships between the current distribution of species and key attributes whereas process-based models simulate ecosystem and tree species dynamics based on representations of physical and biological processes. TreeAtlas, which uses DISTRIB SDM, and Linkages and LANDIS PRO, process-based ecosystem and landscape models, respectively, were used concurrently on four regional climate change assessments in the eastern Unites States.Objectives
We compared predictions for 30 species from TreeAtlas, Linkages, and LANDIS PRO, using two climate change scenarios on four regions, to derive a more robust assessment of species change in response to climate change.Methods
We calculated the ratio of future importance or biomass to current for each species, then compared agreement among models by species, region, and climate scenario using change classes, an ordinal agreement score, spearman rank correlations, and model averaged change ratios.Results
Comparisons indicated high agreement for many species, especially northern species modeled to lose habitat. TreeAtlas and Linkages agreed the most but each also agreed with many species outputs from LANDIS PRO, particularly when succession within LANDIS PRO was simulated to 2300. A geographic analysis showed that a simple difference (in latitude degrees) of the weighted mean center of a species distribution versus the geographic center of the region of interest provides an initial estimate for the species’ potential to gain, lose, or remain stable under climate change.Conclusions
This analysis of multiple models provides a useful approach to compare among disparate models and a more consistent interpretation of the future for use in vulnerability assessments and adaptation planning.12.
Dennis N. Lozada R. Esten Mason Md Ali Babar Brett F. Carver Gina-Brown Guedira Keith Merrill Maria Nelly Arguello Andrea Acuna Lucas Vieira Amanda Holder Christopher Addison David E. Moon Randal G. Miller Susanne Dreisigacker 《Euphytica》2017,213(9):222
Genome-wide association studies (GWAS) are useful to facilitate crop improvement via enhanced knowledge of marker-trait associations (MTA). A GWAS for grain yield (GY), yield components, and agronomic traits was conducted using a diverse panel of 239 soft red winter wheat (Triticum aestivum) genotypes evaluated across two growing seasons and eight site-years. Analysis of variance showed significant environment, genotype, and genotype-by-environment effects for GY and yield components. Narrow sense heritability of GY (h 2 = 0.48) was moderate compared to other traits including plant height (h 2 = 0.81) and kernel weight (h 2 = 0.77). There were 112 significant MTA (p < 0.0005) detected for eight measured traits using compressed mixed linear models and 5715 single nucleotide polymorphism markers. MTA for GY and agronomic traits coincided with previously reported QTL for winter and spring wheat. Highly significant MTA for GY showed an overall negative allelic effect for the minor allele, indicating selection against these alleles by breeders. Markers associated with multiple traits observed on chromosomes 1A, 2D, 3B, and 4B with positive minor effects serve as potential targets for marker assisted breeding to select for improvement of GY and related traits. Following marker validation, these multi-trait loci have the potential to be utilized for MAS to improve GY and adaptation of soft red winter wheat. 相似文献
13.
Cell cultures infected with BHV-1/F(syn), a recombinant bovine herpesvirus 1 (BHV-1) which expresses a synthetic open reading frame encoding the fusion (F) protein of the bovine respiratory syncytial virus (BRSV), showed a cytopathic effect (CPE) indistinguishable from that induced by wildtype BHV-1 although transient transfection experiments demonstrated that expression of the F protein leads to formation of large syncytia. Since it has been shown that glycoprotein M (gM) of pseudorabies virus inhibits BRSV F-induced syncytium formation in transient plasmid transfection experiments [Pseudorbies virus glycoprotein M inhibits membrane fusion. J. Virol. 74 (2000) 6760], the gM ORF of wtBHV-1 and BHV-1/F(syn) was interrupted. Infection of cell cultures with the resulting gM(-) mutant of BHV-1/F(syn) led to formation of syncytia, whereas the CPE in gM(-)BHV-1 infected cells was comparable to the CPE in wtBHV-1 infected cultures. Our results demonstrate that gM is not essential for BHV-1 replication in cell culture and that gM is involved in inhibition of the cell fusion activity of the BHV-1 expressed BRSV F protein. 相似文献
14.
15.
ASSESSING CIRCLE OF WILLIS BLOOD CIRCULATION IN DOGS WITH TRANSCRANIAL COLOR-CODED DUPLEX SONOGRAPHY
Francisco J. Duque Jose M. Dominguez-Roldan Patricia Ruiz Concepcion Zaragoza Rafael Barrera-Chacon 《Veterinary radiology & ultrasound》2009,50(5):530-535
Insonation of Circle of Willis by transcranial Doppler duplex color sonography is described in 30 healthy dogs with 15 weighing <33 lb and 15 weighing >33 lb. Imaging was via a temporal window to explore the rostral, middle, and caudal cerebral arteries on both the left and right-hand sides; and through an suboccipital window to study the basilar artery. Normal mean values of the peak systolic velocity (PSV), end diastolic velocity, mean velocity, resistance index (RI), and pulsatility index (PI) were characterized and compared with those obtained in previous studies. There was significant differences in the PSV, RI, and PI in the rostral cerebral artery between dogs weighing <33 vs. >33 lb. Mean PSV was higher in weighing over 33 lb, whereas the mean resistive index and mean PI were lower in these dogs. 相似文献
16.
17.
Patricia Ferreira-Neves Stéphane Lezmi Typhaine Lejeune Farasao Rakotovao Claire Dally Jean-Jacques Fontaine Florence Bernex Nathalie Cordonnier 《Journal of veterinary diagnostic investigation》2008,20(1):110-114
A hepatic mass was identified in a 5-year-old, female mixed-breed cat that died spontaneously after a clinical history of progressive emaciation, ptyalism, and persistent coryza. At necropsy, a 7-cm-diameter, yellow-brown, firm, multilobulated tumor was identified in the liver. Microscopically, the mass consisted of neoplastic cells arranged in small, closely packed nests within a thin fibrovascular stroma. These cells were of medium sized and polygonal, with fine argyrophilic cytoplasmic granules. Nuclei were predominantly round with finely stippled chromatin and indistinct nucleoli. Mitotic figures were numerous. Immunohistochemically, most of the neoplastic cells were immunoreactive for chromogranin A, neuron-specific enolase (NSE), and cytokeratin AE1/AE3 and weakly labeled for synaptophysin. The tumor was negative for glial fibrillary acidic protein (GFAP), vimentin, and cytokeratins 5, 6, 8, and 17. Vascular emboli and intrahepatic micrometastasis were also identified with chromogranin A. All these features were consistent with a hepatic neuroendocrine carcinoma and emphasized the importance of using a panel of antibodies to diagnose such rare tumors. 相似文献
18.
Anna Catharina B Berge Elizabeth Thornburg John M Adaska Robert B Moeller Patricia C Blanchard 《Journal of veterinary diagnostic investigation》2008,20(4):497-500
The present study describes antimicrobial resistance patterns of Salmonella enterica subspecies enterica serovar Dublin (S. Dublin) in clinical submissions from calves and temporal and farm-type trends in antimicrobial resistance patterns of the isolates. A total of 300 isolates of S. Dublin were obtained from fecal or internal organs of calves fewer than 120 days of age originating from 84 dairies and 18 calf ranches from July 1998 to December 2002. The isolates were susceptibility tested to a panel of 10 antimicrobials using the disk diffusion assay. Temporal and farm-type trends in individual antimicrobial inhibition zone sizes were assessed and antimicrobial resistance patterns were described using cluster analysis. Isolates obtained from calf ranches compared with dairies exhibited decreased susceptibility to florfenicol, gentamicin, neomycin, sulfisoxazole, sulfamethoxazole/trimethoprim, and tetracycline. During the years 1998-2002, decreasing susceptibility was seen for ceftiofur, enrofloxacin, and sulfamethoxazole/trimethoprim. There were 20 different antimicrobial resistance patterns in the isolate set, indicating that S. Dublin has the ability to transfer and pick up resistance genes with relative ease. The trends seen in antimicrobial resistance in S. Dublin may likely be linked to antimicrobial drug use in young calves. 相似文献
19.
Gavin W. Glenney Patricia A. Barbash John A. Coll 《Journal of aquatic animal health》2016,28(1):56-67
Epizootic epitheliotropic disease virus (EEDV; salmonid herpesvirus [SalHV3]; family Alloherpesviridae) causes a systemic disease of juvenile and yearling Lake Trout Salvelinus namaycush. No cell lines are currently available for the culture and propagation of EEDV, so primary diagnosis is limited to PCR and electron microscopy. To better understand the pervasiveness of EEDV (carrier or latent state of infection) in domesticated and wild Lake Trout populations, we developed a sensitive TaqMan quantitative PCR (qPCR) assay to detect the presence of the EEDV terminase gene in Lake Trout tissues. This assay was able to detect a linear standard curve over nine logs of plasmid dilution and was sensitive enough to detect single-digit copies of EEDV. The efficiency of the PCR assay was 99.4 ± 0.06% (mean ± SD), with a 95% confidence limit of 0.0296 (R2 = 0.994). Methods were successfully applied to collect preliminary data from a number of species and water bodies in the states of Pennsylvania, New York, and Vermont, indicating that EEDV is more common in wild fish than previously known. In addition, through the development of this qPCR assay, we detected EEDV in a new salmonid species, the Cisco Coregonus artedi. The qPCR assay was unexpectedly able to detect two additional herpesviruses, the Atlantic Salmon papillomatosis virus (ASPV; SalHV4) and the Namaycush herpesvirus (NamHV; SalHV5), which both share high sequence identity with the EEDV terminase gene. With these unexpected findings, we subsequently designed three primer sets to confirm initial TaqMan qPCR assay positives and to differentiate among EEDV, ASPV, and NamHV by detecting the glycoprotein genes via SYBR Green qPCR.
Received April 20, 2015; accepted November 10, 2015 相似文献
20.
Patricia A Heine 《Veterinary Clinics of North America: Small Animal Practice》2004,34(2):379-395
This review of the anatomy is meant to serve as a quick reference to provide the reader with a concise yet thorough overview of the anatomy of the inner, middle, and external ear as well as the physiology of hearing. Additional information is also presented regarding structures located adjacent to the ear that may be important with respect to surgical anatomy. 相似文献