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11.
Ken Overturf Roger L. Vallejo Yniv Palti Fredrick T. Barrows James E. Parsons 《Aquaculture International》2012,20(2):213-232
Microarray analysis was conducted using liver samples from two families of rainbow trout that differed in their growth responses
when compared between individuals fed a fishmeal or plant protein-based diet. Differential expression relating to dietary
utilization between the two families found significant changes in expression of 33 expressed sequence tags (ESTs). Eight of
the differentially expressed ESTs had identified mammalian homologs that had been previously researched with identified cellular
interactions and functions. Utilizing pathway analysis software to analyze sequences annotated with known mammalian genes,
we were able to map gene pathways and process interactions. From this information, we were able to infer that the metabolic
changes associated with utilization of plant protein versus fishmeal were associated with differential regulation of genes
related to cell oxidative stress, proliferation, growth and survival. Furthermore, we inferred from the changes we observed
in immune response gene expression that ingestion of this plant-based diet upregulated the expression of genes involved in
immunoregulatory processes. 相似文献
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Effects of dietary change and rotavirus infection on small intestinal structure and function in gnotobiotic piglets 总被引:1,自引:0,他引:1
G A Hall K R Parsons G L Waxler K J Bunch R M Batt 《Research in veterinary science》1989,47(2):219-224
The combined effects of weaning and rotavirus infection on small intestinal structure and function and on growth rate were studied in 28 gnotobiotic piglets. There was little damage by rotavirus to the proximal small intestine, some damage to the mid small intestine and relatively severe damage to the distal small intestine; villi were stunted, crypts lengthened and activities of all brush border enzymes decreased. The damage was short-lived despite the synchronisation of rotavirus infection with simulated weaning. There was no evidence of persistent damage to the small intestine and growth rate was unaffected. 相似文献
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J H Parsons 《The Veterinary record》1970,87(13):367-370
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Rotaviruses are enteric pathogens of cattle but sub-clinical infections are common. Virulence variation has been identified with bovine rotaviruses and some rotaviruses replicated without clinical signs in non-immune calves. The rotavirus genome is composed of eleven segments of double-stranded RNA and the fourth largest segment codes for a non-glycosylated surface protein, VP4, which has been linked with virulence. In the present study the biological basis of rotavirus virulence variation was studied in vivo and compared with the known properties of the fourth gene. Calves were inoculated orally with a virulent rotavirus or a rotavirus of low virulence which multiplied but failed to cause diarrhoea. They were taken for necropsy at intervals of 2 days after inoculation. Clinical signs, virus in faeces and the percentage of infected small intestinal epithelium were determined. Damage to the small intestine was assessed by measurement of villus heights and crypt-cell production rates. Virulence was associated with a greater level of colonization of the small intestinal epithelium, greater enterocyte damage and preferential infection of the upper small intestine. The fourth gene determines the ability of rotaviruses to spread in vitro and the finding that virulence was associated with greater colonization in vivo raises the possibility that this gene may have an important role in rotavirus virulence. 相似文献
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R. W. Skaggs M. A. Brevé A. T. Mohammad J. E. Parsons J. W. Gilliam 《Irrigation and Drainage Systems》1995,9(3):259-277
The design and management of drainage systems should consider impacts on drainage water quality and receiving streams, as well as on agricultural productivity. Two simulation models that are being developed to predict these impacts are briefly described. DRAINMOD-N uses hydrologic predictions by DRAINMOD, including daily soil water fluxes, in numerical solutions to the advective-dispersive-reactive (ADR) equation to describe movement and fate of NO3-N in shallow water table soils. DRAINMOD- CREAMS links DRAINMOD hydrology with submodels in CREAMS to predict effects of drainage treatment and controlled drainage losses of sediment and agricultural chemicals via surface runoff. The models were applied to analyze effects of drainage intensity on a Portsmouth sandy loam in eastern North Carolina. Depending on surface depressional storage, agricultural production objectives could be satisfied with drain spacings of 40 m or less. Predicted effects of drainage design and management on NO3-N losses were substantial. Increasing drain spacing from 20 m to 40 m reduced predicted NO3-N losses by over 45% for both good and poor surface drainage. Controlled drainage further decreases NO3-N losses. For example, predicted average annual NO3-N losses for a 30 m spacing were reduced 50% by controlled drainage. Splitting the application of nitrogen fertilizer, so that 100 kg/ha is applied at planting and 50 kg/ha is applied 37 days later, reduced average predicted NO3-N losses but by only 5 to 6%. This practice was more effective in years when heavy rainfall occurred directly after planting. In contrast to effects on NO3-N losses, reducing drainage intensity by increasing drain spacing or use of controlled drainage increased predicted losses of sediment and phosphorus (P). These losses were small for relatively flat conditions (0.2% slope), but may be large for even moderate slopes. For example, predicted sediment losses for a 2% slope exceeded 8000 kg/ha for a poorly drained condition (drain spacing of 100 m), but were reduced to 2100 kg/ha for a 20 m spacing. Agricultural production and water quality goals are sometimes in conflict. Our results indicate that simulation modeling can be used to examine the benefits of alternative designs and management strategies, from both production and environmental points-of-view. The utility of this methodology places additional emphasis on the need for field experiments to test the validity of the models over a range of soil, site and climatological conditions. 相似文献
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