敦煌西晋画像砖所绘“白象”形象溯源

敦煌佛爷庙湾西晋早期墓出土的白象画像砖,是佛教在当时被视为一种神秘性方术而存在的现实表现。本文对敦煌西晋画像所绘“白象”形象进行溯源,认为画像砖上的六牙白象应该是中国早期佛教朝着神秘主义方向发展的一个缩影。     

江西省农业数字园区信息系统的架构与实现

农业数字园区JPDAMIS将成为农业生产新的信息源,任何与农业生产有关的信息,都将被定位,并与空间数据库联系起来。用户可以多媒体的形式查询农业生产信息,获得所需的信息。为此,介绍了建立江西省农业数字园区信息系统的必要性和实现,及其应用和前景     

Correlation between progression of atherosclerosis accelerated by emotional stimulation and imbalance of SDF-1/CXCR4 in mice

AIM To observe effects of emotional stimulation on expression of stromal cell-derived factor-1（SDF-1） and CXC chemokine receptor 4 （CXCR4） in plasma, platelets, aortas, hippocampus and bone marrow of apolipoprotein E gene knockout （ApoE^-/-） mice, and to reveal the possible mechanism of the aggravated atherosclerotic plaque vulnerability by emotional stimulation. METHODS Thirty 8-week-old male ApoE^-/- mice were randomly divided into normal control group, high fat group, and emotional stimulation group. Ten 8-week-old inbred C57BL/6J mice served as blank control group. After 12 weeks of intervention, the serum levels of SDF-1 and CXCR4 were investigated by ELISA. The protein levels of SDF-1 and CXCR4 in platelets, aortas, hippocampus and bone marrow were determined by Western blot. The pathological damage of aortas was observed by oil red O staining. RESULTS Compared with blank control group, normal control group and high fat group, the mice subjected to emotional stimulation showed more serious atherosclerosis in aortas detected by oil red O staining, and increased levels of SDF-1 and CXCR4 in the plasma and aortas were also observed （P<0.05）. The results of Western blot showed that the protein levels of SDF-1 and CXCR4 in platelets, aortas and hippocampus were increased in the mice subjected emotional stimulation, but the expression of SDF-1 and CXCR4 in the bone marrow was decreased （P<0.05）. CONCLUSION Imbalance of SDF-1/CXCR4 may be the key target by which emotional stimulation accelerates the progression of atherosclerosis.     

藁本属药用植物分子生物学研究进展

藁本属内大多植物为我国传统中药材,具有活血行气、散寒、除湿、祛风止痛的作用.然而目前对藁本属植物的研究大多集中在栽培种植技术、药用成分价值等方面,分子技术研究较少,研究面比较窄,导致对其更进一步的分子育种、药效成分的基因定位等研究进展缓慢.该研究归纳国内外藁本属植物的遗传多样性、DNA基因鉴别、功能基因克隆、转录组等研究进展,分析了藁本属植物分子生物学研究的不足,并对未来研究应关注的方向进行了展望,以期为藁本属植物的分子生物学研究提供参考依据.     

慈利县茶叶生产的现状与发展对策分析

慈利县产茶历史悠久。据西晋《荆州土地记》载：“武陵七县通出茶,最好。”《直隶澧州志林》之《云朝山记》有“云朝山邑（慈利）南九十里,高数千仞,高顶种有茶树,青香异味,昔人评之日云雾仙品。”1982年以前,全县有25个乡镇产茶,占乡镇总数的70％。现在该县茶园主要集中于三合口乡、庄塔乡、象市镇、江垭镇、国太桥乡等5个乡镇,面积1600hm^2,涉及茶农近4万人,是慈利县北部山区重要的经济作物与收入来源。     

饲用复合酶对蛋用种鸡日粮磷的利用和生产性能的影响

本试验旨在研究低磷日粮添加复合酶制剂对蛋用种鸡磷的利用和生产性能的影响。选用972只24周龄罗曼褐父母代种鸡,分为3个处理,每处理6个重复,每重复54只种鸡。采用玉米-豆粕-杂粕型基础饲粮,正对照组含总磷0.55%,非植酸磷0.40%,不加酶;负对照组含总磷0.40%,非植酸磷0.21%,不加酶;试验组含总磷0.40%,非植酸磷0.21%,加酶制剂。试验期18周。结果表明:在低非植酸磷的日粮中添加复合酶,粪便中磷含量极显著低于正对照组(P<0.01);粪便中粗蛋白质含量显著低于正对照组和负对照组(P<0.05);显著改善蛋黃顔色(P<0.05);不影响生产性能、骨骼发育和蛋壳质量。添加200 g/t复合酶替代罗曼褐蛋用种鸡日粮中75%的磷酸氢钙能够降低饲料成本,提高养殖企业的经济效益,对罗曼褐蛋用种鸡生产性能无不利影响。     

金龟子绿僵菌CQMa421对棉蚜、绿盲蝽的田间防效

棉花是我国重要的经济作物, 虫害严重制约了棉花生产, 造成棉农的经济损失。目前, 化学杀虫剂仍是防治棉花虫害的主要手段, 长期使用化学杀虫剂导致棉蚜Aphis gossypii、绿盲蝽Apolygus lucorum产生抗药性, 降低防治效果。本研究使用80亿孢子/mL金龟子绿僵菌CQMa421可分散油悬浮剂防治棉蚜、绿盲蝽, 室内生测结果表明, 金龟子绿僵菌CQMa421能有效侵染棉蚜和绿盲蝽, 随着浓度的升高, 对棉蚜和绿盲蝽的毒力增强。〖JP+2〗田间试验结果表明, 该药剂能有效控制棉花主要害虫棉蚜和绿盲蝽。采用1 200 mL/hm2用量, 药后14 d对棉蚜的防效达94.42%; 采用1 350 mL/hm2用量, 药后14 d对绿盲蝽的防效分别达到87.25%(新疆), 91.62%(湖北)。     

基于形态性状和SSR标记的克氏原螯虾养殖群体遗传多样性分析

为客观评估广西南宁周边地区克氏原螯虾养殖群体的遗传多样性,采集来自南宁的3个克氏原螯虾群体——埃及群体（AJ）、良庆群体（LQ）和上林群体（SL）,以及湖北荆州（JZ）和江西湖口（HK）的群体各1个,采用形态学结合微卫星分子标记的方法分析其遗传多样性水平。形态分析结果显示,雌性群体的综合判别准确率为68.59%,雄性群体的综合判别准确率为73.60%;3个南宁群体（AJ,LQ,SL）与JZ群体聚为一类,3个南宁群体间具有较高的形态相似度。微卫星分析结果显示,AJ群体和LQ群体的遗传多样性最高,SL群体和HK群体次之;LQ群体与SL群体的遗传分化系数和遗传距离最小。结果表明广西南宁克氏原螯虾群体具有较高的遗传多样性,从国外引种或国内不同地理来源群体的杂交可能是提高克氏原螯虾群体遗传多样性的重要途径。     

Sulodexide protects against hyperglycemia-induced retinal vascular injury via suppressing NOX4/NLRP3 pathway

AIM To investigate the effect of sulodexide (SDX) on high glucose-induced damage in retinal microvascular endothelial cells. METHODS (1) High-fat diet combined with intraperitoneal injection of streptozocin were used to induce type 2 diabetes mellitus (DM) followed by injection of saline or SDX in C57BL/6J male mice. Retinal microvascular leakage and density, and the protein levels of NLRP3 inflammasome-related proteins, zonula occludens-1 (ZO-1) and NADPH oxidase 4 (NOX4) were measured. (2) Human retinal microvascular endothelial cells (HRMECs) were treated with normal glucose or high glucose with or without SDX, and were further transfected with siRNA to knock down NOX4, or infected by adenovirus to over-express NOX4. The protein levels of ZO-1, VE-cadherin (VE-Cad), NOX4 and NLRP3 inflammasome-related proteins as well as the level of reactive oxygen species (ROS) were detected. RESULTS Treatment with SDX increased the protein level of ZO-1, attenuated retinal leakage and NLRP3 inflammasome activation, and enhanced the density of microvasculature and the number of ganglion cells in diabetic retinas. The protein levels of ZO-1 and VE-Cad were decreased, while the levels of NOX4, NLRP3 inflammasome-related proteins and ROS generation were increased in high glucose-treated HRMECs. Silencing of NOX4 inhibited high glucose-induced increases in NLRP3 inflammasome and ROS generation, and decreases in the protein levels of ZO-1 and VE-Cad. Over-expression of NOX4 significantly increased the levels of NLRP3 inflammasome-related proteins and ROS generation in HRMECs, and reduced the protein levels of ZO-1 and VE-Cad. Treatment with SDX partly reversed NOX4 over-expression-induced changes. CONCLUSION SDX alleviates hyperglycemia-induced retinal microvascular endothelial injury via inhibiting NOX4/ROS/NLRP3 pathways.