富含多糖的强旱生植物半日花基因组DNA提取方法

半日花(Helianthemum soongoricum)属强旱生植物,其组织富含多糖和多酚等次生代谢物质,采用常规CTAB和SDS等方法提取的半日花基因组DNA由于含有多糖等杂质,无法进行PCR扩增和限制性酶切等试验,为此,在CTAB法的基础上进行了改进。第1步通过向植物组织CTAB提取液中添加高盐和乙醇来沉淀部分多糖,第2步将获得的DNA粗提液,经过CTAB分离溶液沉淀、NaCl溶解、乙醇沉淀等方法,可有效去除半日花组织中的残余多糖等杂质,提取DNA的质量和纯度经凝胶电泳条带清晰、无降解、无多糖等杂质。PCR扩增和限制性内切酶的结果表明,通过改进的方法所获得的DNA,可以进行PCR扩增试验,扩增条带清晰,基因组DNA能被限制性内切酶完全酶切,该方法为后续半日花分子生物学研究奠定了重要基础。     

Research on a Method for Purification and Multiplication of PTGMS Lines

[目的]研究一种光温敏核不育系提纯繁殖技术。[方法]利用生产用种安农810S、P88S和 C815S三个光温敏核不育系,第1年通过分蔸建立2个遗传背景完全相同的不育系大群体,实现核心单株筛选与繁殖同步进行。所得核心种子于当年在海南严格隔离条件下冬繁获得原原种,并从中筛选优良单株,作为下一年2个遗传背景完全相同供试群体用种。第2年在长沙正季种植所得用种,继续核心单株筛选与繁殖同步进行。所得核心种子又在海南冬繁获得原原种,以后每年重复上述步骤。[结果]安农810S、P88S和 C815S群体中核心单株的比例由第一年的40.3%、0.7%和0.3%分别提升到第二年的71.67％、66.00％和68.67％。[结论]该方法经过连续多年的不育系提纯繁殖,能有效控制不育系起点温度“遗传漂移”,同时得到高纯度、不育起点温度能有效控制的核心种子。     

Effects of Ligustrazine Combined with Cis- dichlorodiamine Platinum on Anti-proliferation,Cell Cycle and Apoptosis of Human Gastric Carcinoma SGC-7901 Cell Lines

[Objective] This study aimed to investigate the combination effects of ligustrazine and cis-dichlorodiamine platinum （DDP） on anti-proliferation, cell cycle and apoptosis of SGC-7901 cell lines in vitro. [Methed] SGC-7901 cells were treat- ed with ligustrazine and DDP alone or combined for 48 h for morphology assay. Anti-proliferative effects with the same treatment for 24, 48 and 72 h were assayed by MTT method, respectively. Cell cycle distribution and apoptosis assay of treated cells were performed in flow cytometry. Zhengjun Jin＇s protocol was used to assay the effect of drug combination. [Result] Ligustrazine significantly increased the prolif- eration inhibition rate of DDP on SGC-7901 cells in combination with DDP, com- paring with the effects of ligustrazine or DDP alone, and exhibited synergistic antitu- mor effect. The combination drug treatment induced cell cycle arrest occurred in S and G2 phase of the cell cycle and increased the apoptosis rate significantly. [Conclusion] Our results indicate that ligustrazine, as a low-toxic and natural herbal component, can significantly increase the anti-proliferative effect and apoptosis rate of antitumor drug DDP on human gastric carcinoma SGC：.-7901 cells.     

几种生态因子对偏顶蛤浮游幼虫生长和发育的影响

为了解偏顶蛤Modiolus modiolus最适培育条件,采用实验生态学方法研究了海水盐度、 pH和饵料对偏顶蛤浮游幼虫生长和发育的影响。结果表明：3种生态因子对偏顶蛤浮游幼虫的生长发育均有显著影响(P<0·05);幼虫生长发育的适宜盐度为25~35,最适盐度为30,但幼虫对高盐度的耐受力比对低盐度的耐受力强;幼虫生长发育的适宜pH为6~9,最适pH为8;饵料试验中,湛江等鞭金藻Isochrysis zhan：jiangensis、新月菱形藻Nitzschia closterium和牟氏角毛藻Chaetoceros muelleri Lemmerman 3种饵料混合投喂的效果最好,牟氏角毛藻单独投喂以及牟氏角毛藻与新月菱形藻混合投喂的效果最差,湛江等鞭金藻基本上能够满足偏顶蛤早期浮游幼虫生长发育的营养需求,单一投喂湛江等鞭金藻和新月菱形藻组与投喂含金藻的混合饵料组对幼虫的饲喂效果无显著性差异(P>0·05)。     

储罐机械清洗技术的改进措施

针对传统的储罐机械清洗设备及其技术在部分环节上的不足,提出了具体的改进措施：将一罐增加为两罐,使离心泵始终在常压状态下工作;将制氮机与氧气检测仪联动运行,以安全控制罐内氧气的体积分数;将油水分离装置改成自动密闭撇油容器,以防止油气扩散,降低人员劳动强度;安装气动控制阀门,避免人员在油罐浮顶上切换清洗机,增强了安全保障。通过真空回收、监测、撇油、清洗机切换等技术改进和创新,引导油罐底泥清洗技术向高效化、密闭化、自动化的方向发展,值得普遍应用与推广。     

微乳透皮给药机制及影响吸收的因素

微乳能够提高药物溶解度,作用于皮肤角质层、皮肤附属器进而促进药物的透皮吸收,影响药物透皮吸收的因素主要是微乳中的各个组分以及所承载的药物特性。微乳用于经皮给药制剂将会有更广阔的应用前景和发展空间。文章综述了微乳透皮给药的吸收机制以及影响微乳透皮吸收的因素。     

UPLC法同时测定人参糖中7种人参皂苷

建立同时测定人参糖中7种人参皂苷含量的快速检测方法。采用超高效液相色谱法,ACQUITY UPLC BEH C18色谱柱(2.1×50mm,1.7μm),以乙腈-水为流动相,梯度洗脱,流速0.5mL/min,柱温35℃,进样量2μL,检测波长203nm。人参皂苷Rg1、Rf、Re、Rb1、Rc、Rb2、Rd均达到基线分离,线性良好,平均回收率分别为98.26%、98.46%、97.00%、103.75%、96.32%、95.71%、95.00%。该方法准确、重现性好,可用于人参糖中人参皂苷含量测定。     

纳米氧化铈颗粒对大鼠肺组织 GRP78表达的影响

探讨不同粒径纳米氧化铈颗粒对大鼠肺组织的毒性效应及对大鼠肺组织 GRP78蛋白表达的影响,将粒径分别为35 nm±3 nm、287 nm±27 nm 的纳米氧化铈颗粒悬液以400 mg／kg 剂量对大鼠灌肺。染毒28 d 后,采用免疫组化法检测大鼠肺组织 GRP78蛋白表达情况。结果表明,与对照组相比,不同粒径纳米氧化铈细胞 GRP78蛋白表达均增加（P ＜0．05）,且35 nm±3 nm 组高于287 nm±27 nm 组（P ＜0．05）。说明不同粒径纳米氧化铈均可引起大鼠肺组织 GRP78蛋白表达增加,且粒径越小,表达越高。     

鸭坦布苏病毒和鸭瘟病毒二重荧光定量RT-PCR方法的建立

The study was conducted to establish the duplex Real-time PCR assay for detecting both duck tembusu virus (DTMUV) and duck plague virus (DPV). According to the sequences of DTMUV E gene and DPV UL6 gene in GenBank, two sets of specific oligonucleotide primers for DTMUV and DPV along with two TaqMan probes were designed. The duplex Real-time PCR assay was developed through optimization of reaction conditions and validation of specificity, sensitivity and repetitiveness of the method. The sensitivity of the assay were both 100 template copies for DTMUV and DPV. There was no specific bands of the same sizes were amplified from other duck pathogens, such as duck Newcastle disease virus, duck hepatitis virus, muscovy duck parvovirus, duck circovirus, H9 subtype avian influenza virus, egg drop syndrome virus. This duplex Real-time RT-PCR assay is a sensitive, quick, specific and quantitative test for detection of DTMUV and DPV, and will be useful for the control of these viruses in ducks.     

Effects of Oral Echinacea purpurea on Heart Rate，ECG,Blood Pressure and Respiration of Awake Beagle Dogs

The experiment studied the effects of oral Echinacea purpurea on heart rate, ECG, blood pressure and respiration of awake Beagle dogs. Experimental results showed that oral Echinacea purpurea 0.125 to 1.25 g/kg could apparently reduced the heart rate of awake Beagle dogs, and had no significant effects on blood pressure, respiration and ECG between P wave, T wave, R wave, QT interval, PR interval as well as QRS duration （P>0.05）. It showed that Echinacea purpurea had no apparent effects on the dog’s respiratory system and cardiovascular system, which was safe and reliable in application doses.