A method for measuring adenosine triphosphate in soil

A method was devised for the extraction and measurement of adenosine 5'-triphosphate (ATP) in soil that minimizes sorption of ATP on the soil colloids. Soil was ultrasonified for 1 min with a solution containing trichloracetic acid (0.5 m). disodium hydrogen orthophosphate (0.25 m) and paraquat dichloride (0.1 m). The ATP content of the filtered extract was determined without further treatment in a scintillation spectrometer by the firefly luciferin-luciferase system. Recovery of added ATP was greater using the extratant containing trichloracetic acid, orthophosphate and paraquat than with trichloracetic acid alone or with a sulphuric acid extradant. Recoveries of added ATP ranged from 45% to 84% in thirteen different soils; ATP contents from 0.64 to 9.03 μg g^?1 soil.     

Biodegradation of litter in Eucalyptus pauciflora communities—II. Fungal succession in fungicide- and insecticide-treated leaves

Fungal succession on untreated and fungicide- and insecticide-treated leaves of Eucalyptus pauciflora was compared. Natural succession on untreated leaves fits the general scheme for succession on deciduous tree leaves. Initial colonizers are species of Coelomycetes and Moniliales. When the numbers of Coelomycetes decrease in the late stages of decomposition, the frequency of Penicillium species and species of Zygomycetes increases. The succession is affected only marginally by DDT and dieldrin treatment while benomyl severely restricts the occurrence of most fungal species. The effect of benomyl persists even after exposure for 48 weeks in the field.     

Some problems in assessing the fluorescein diacetate-active fungal biomass in the soil

A technique for assessing the metabolically-active fungal biomass by means of fluorescein diacetate (FDA) staining was recently described (Söderström, 1977). In this note some practical details in the application of this technique to studies of FDA-active fungal biomass in the soil are discussed.     

Calcium oxalate accumulation and soil weathering in mats of the hypogeous fungus Hysterangium crassum

Fungal mats of Hysterangium crassum (Tul. and Tul.) Fischer occupied a mean of 9.6% of the upper 10 cm of soil developed under a 40–65 yr old stand of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) in Oregon. This hypogeous basidiomycete exudes large amounts of oxalic acid, some of which precipitates with Ca in microscopic crystals of calcium oxalate, resulting in a mean CaC₂O₄ content of 82g m^?2 for the entire soil. Soil oxalate concentration was significantly greater within fungal mats (P < 0.01) and soil pH was significantly lower (P < 0.01) than in soil adjacent to mats. The quantity of Ca present as CaC₂O₄ is 0.5 the amount of exchangeable Ca in the soil and exceeds the mass of Ca lost annually in runoff. Scanning electron micrographs show intense chemical weathering, attributable to oxalate attack, in the immediate vicinity of hyphae. X-ray diffraction patterns of clay indicate bulk weathering is more intense within the fungal mats than in adjacent uncolonized soil.     

Interactions between bacteria and ectomycorrhizal fungi

Interactions between eight ectomycorrhizal fungi and eight bacteria were tested on five laboratory media and in the rhizoplane of Pinus radiata. Depression of growth of the fungi by the bacteria in laboratory media was dependent on the medium and bore little relation to effects in the rhizoplane. In the rhizoplane, different bacteria could depress, have no effect or even stimulate growth of mycorrhizal fungi. Competition and antagonism are suggested as mechanisms for depression of the fungi. Some bacteria gave protection against the depressive effects of other bacteria. Considerable differences occurred between ectomycorrhizal fungi in their colonization of the rhizoplane in the absence of bacteria and also in their presence. The common mycorrhizal fungi Rhizopogon luteolus and Thelephora terrestris generally colonized roots well but the strain of Pisolithus tinctorius studied colonized poorly. Direct microscopy showed the percentage cover of the root by microorganisms was usually only 10–20%.It is proposed that interactions of ectomycorrhizal fungi with soil organisms are important in determining the successful introduction and persistence of inoculated ectomycorrhizal fungi. Fungi should be selected for compatibility with a wide range of soil microflora as well as efficiency in plant stimulation.     

Distribution and partial characterisation of acid hydrolysable organic nitrogen in six New Zealand soils

Six New Zealand topsoils of widely different origins and properties were subjected to 6m HC1 hydrolysis and the distribution of N fractions and amino acids were determined qualitatively and quantitatively.Of the total-N in the soils studied 83–91%, was hydrolysable with 6m HCl. The largest proportion of the hydrolysable N was α-amino acid N (38– 42%). followed by hydrolysable-unknown N (HUN) (14–24%), and NH₄⁺-N (14–22%). A significant proportion (25–50%) of the HUN fraction was accounted for by the non α-amino acid-N. Oxidative (3% H₂O₂) hydrolysis released N-phenoxy amino acid-N and possibly N-compounds which were complexed with phenols and sugars. All soils had a similar amino-acid composition with a predominance of acidic amino-acids.     

Colonization by Rhizobia of the seed and roots of legumes in relation to exudation of phenolics

A study of the colonization pattern of Rhizobium inoculated either in the soil or on to the seeds of green gram (Phaseolus aureus Roxb.), black gram (P. mungo L.) and sun hemp (Crotalaria juncea L.) revealed a significant reduction of Rhizobium in the spermosphere of sun hemp which was attributed to excretion of phenolic compounds by the seed. As the plants aged, the quantity of phenolics exuded by the roots was reduced and there was a parallel increase in the Rhizobium population in the rhizospheres of the three plant species.     

Remarks on the anatomy and ontogeny of scales of teleosts. I

Densitometer records were made from the scales of a number of fish species, mainly from pikeperch. Analysis of the records showed that the pattern of hollows, representing the circuli in the curves, was very regular. No arrangement into groups of narrowly interspaced circuli could be demonstrated. It was therefore concluded that the circuli of the surface layer do not produce the “growth-checks”. Ringlike structures were also present in the fibrous basal plate. These were about 3–5 μm closer together than the circuli. These two circular gratings of slightly different inter-ring spacing will create an interference pattern, in this case a circular moiré pattern, having no direct relation to the structures present in the two layers forming the scale.     

Comparison of yeast and algal diets for bivalve molluscs

Four species of bivalve molluscs were fed diets consisting of varying proportions of the yeast Candida utilis and the diatom Thalassiosira pseudonana. Juvenile Argopecten irradians, Mercenaria mercenaria, and Mytilus edulis grew as fast or faster than controls when fed diets containing as much as 50% yeast. Growth of soft tissue in Crassostrea virginica, however, decreased with the amount of yeast in the diet. The relative food values of the different diets were not closely correlated with gross chemical composition or amino acid composition.     

Glugea heraldi n. sp. (Microsporida, Glugeidae) from the seahorse Hippocampus erectus Perry

Abstract. A new species of Microsporida, Glugea heraldi , is described from the seahorse Hippocampus erectus . Measurements of fixed spores and notes on the histopathology of parasitized tissue are given.