Carbon monoxide measurements in the troposphere

During the second flight of the space shuttle, the measurement of air pollution from satellites (MAPS) experiment in the OSTA-1 payload acquired approximately 35 hours of radiometric measurements of the carbon monoxide mixing ratio in the middle troposphere, upper troposphere, and lower stratosphere. A gas filter radiometer operating in the 4.67-micrometer band was used to acquire the data over the region from 38 degrees N to 38 degrees S during both daytime and nighttime. The performance of the measurement system was excellent. The data reduced to date indicate the presence of significant gradients in the middle tropospheric carbon monoxide mixing ratio with both latitude and longitude over the North Atlantic, the Mediterranean Sea, and the Middle East. On the basis of comparisons with directly measured values, the accuracy of the measurements is approximately 15 percent. Comparisons of data taken on successive orbits over the same geographic region indicate that the repeatability of the measurements is approximately 5 percent.     

Effects of delay in reapplication of nitrogen fertilizer following cutting silage from a ryegrass sward

Plots of a 2-year-old sward of Merlinda perennial ryegrass received a routine dressing of 100 kg N ha⁻¹ as compound fertilizer in March 1991, followed by further dressings of 100 kg N ha⁻¹ after cuts 1, 2 and 3 in a simulated four-cut silage system, either the same day as cutting or with a delay of 3, 7, 10 or 14 d. Partial irrigation ensured that fertilizer could be taken up immediately. Annual total dry matter yield, the dry matter yield for the experimental cuts 2–4 and their apparent response to N showed no effect of delays of 0–10 d in reapplication of fertilizer, but a significant ( P < 0·01) reduction for 14 d delay. Significantly ( P = 0·001) higher apparent recovery of N in the cut herbage was recorded where reapplication of fertilizer had been delayed by 7 or 10 d (93% and 91% respectively) in comparison with either smaller (0 delay, 78%; 3 d delay, 87%) or greater delay (14 d, 86%). It is suggested that lower recoveries at 0 and 3 d result from the sward's reduced NO₃ uptake following defoliation and that, while in practical situations the conditions may not be as rigorous as those imposed in this experiment, further experiments to determine the fate of N not recovered in the herbage are warranted, so defining management circumstances in which immediate reapplication of N might not be advisable.     

The effect of cooking methods on the lipid composition of potatoes

The effect of boiling and baking in either a convential or microwave oven on the lipid content of potatoes was investigated. The crude lipid content of potatoes was lowered significantly by all methods of cooking. The loss was highest in potatoes cooked in a microwave oven, least in boiled tubers, and intermediate in tubers baked in a conventional oven. Similar trends were observed for both cortex and pith tissues. Generally, the phospholipid content followed the same trend as the crude lipid, the exception being the cortex tissue of tubers baked in the conventional oven, in which the greatest loss of phospholipid occurred. Cooking did not significantly alter the fatty acid composition of tubers. A possible relationship between the degree of cell rupturing, known to be influenced by different mechanisms of heat transfer, and the amounts of tuber lipid associated with different cooking methods was discussed.     

Refinement of an orthotopic lung cancer model in the nude rat

Over 85% of people with lung cancer eventually succumb to this disease, largely because current chemotherapies are ineffective. The testing and validation of promising new approaches generally rely on achieving responses with cell lines in vitro or in tumor xenografts in nude mice. However, quite often the results seen with these models are not recapitulated in the clinic, thus necessitating the need for better animal models of lung cancer for preclinical testing of new therapies. One promising model is that of orthotopic lung cancer, where xenografts of human lung cancer are established in lungs of immunodeficient rodents. The problems associated with this model include poor rates of engraftment, limited tumor multiplicity, and a heightened risk for surgical trauma. The purpose of our study was to develop an efficient approach to engraftment of orthotopic tumors throughout the lungs of the Rowett nude rat. Initially, we augmented immunosuppression in the rats with whole-body X-irradiation and then used orotracheal cannulas to intratracheally instill human cancer cells from the Calu-6 cell line. This protocol produced a low rate of engraftment and low tumor multiplicity. The hypothesis that slight disruption of the pulmonary epithelium or the surfactant layer would allow better tumor engraftment was tested by coadministration of either pancreatic elastase or ethylenediaminetetraacetic acid (EDTA) along with the cell instillations. Lung tumor engraftment was evaluated 8 weeks after instillation. The inclusion of elastase or EDTA with the Calu-6 cells resulted in an 80-100% engraftment rate, respectively. Coadministration of EDTA resulted in significantly larger and greater numbers of tumors/lung than those in elastase-treated animals. Temporal studies demonstrated that small nodules were scattered throughout the lung parenchyma 5 weeks after instilling Calu-6 cells and EDTA. These nodules grew to coalesce and form large masses that effaced >75% of the parenchyma at 9 weeks postinstillation. The refinements made through our studies have led to the development of an orthotopic lung cancer model that should facilitate the evaluation of novel therapies designed to treat or impede lung cancer development.     

Inheritance of genes coding for gliadin proteins and glume colour introgressed into Triticum aestivum from a synthetic wheat

A synthetic hexaploid, Triticum timofeevii×T. tauschii, was used to transfer disease resistance genes to the commercial cultivar Saratovskaya 29 (S29) by backcrossing. After five backcrosses the resulting derivatives still showed some traits of the synthetic, namely brown spike glumes and several gliadin components. Genetic analysis showed that the derived forms had inherited the Gli-D1 allele of the synthetic, which was found to be tightly linked to a gene for glume colour. Recombination percentages between these genes was estimated to be 2.5 ± 1.7%. The development of the derivatives was also accompanied by a rearrangement within the Gli-B1 locus, resulting in the formation of a new variant of the allele in S29.     

Interfacing professional training in health maintenance and preventive medicine.

Because of emerging economic realities of the latter half of this century, the practice of preventive medicine, as opposed to curative medicine, is becoming essential in developed and developing countries. This holds true in veterinary medicine as well as in human medicine, and as we become increasingly aware of this fact, we also recognize the increasing interdependence of both fields. To determine the extent to which veterinarians are prepared to deal with this change in emphasis, two surveys have been prepared; one directed to various specialty groups within the profession and the other directed to schools of veterinary medicine. The first survey was designed to identify areas in which veterinarians felt their training had been deficient relative to decision making in applied preventive medicine. The second survey queried the schools to determine what changes had been made in their curricula to remedy these inadequacies.     

Measured Soil Water Concentrations of Cadmium and Zinc in Plant Pots and Estimated Leaching Outflows from Contaminated Soils

Soil water concentrations of cadmium and zinc were measured in plant pots with 15 contaminated soils which differed in origin, texture, pH (5.1 – 7.8) and concentrations of cadmium (0.2 – 17 mg Cd kg-¹) and zinc (36 – 1300 mg Zn kg-¹). The soil waters contained total concentrations of 0.5 to 17 µg Cd L-¹ and 9 to 3600 µg Zn L-¹, which were dominated by free metal ions as measured by an ion exchange-resin method. Annual leaching outflows were estimated from soil water concentrations to be 0.5 – 17 g Cd ha-¹ y-¹ and 9 – 3600 g Zn ha-¹ y-¹ per 100 mm of net percolation, corresponding to 0.1% per year of the total soil content of cadmium and zinc. The measured soil water concentrations of cadmium and zinc did not correlate linearly with the corresponding soil concentrations but correlated fairly well with concentrations measured in Ca(NO₃)₂ extracts of the soils and with soil water concentrations estimated from soil concentrations and pH. Such concentration estimates may be useful for estimating amounts of cadmium and zinc being leached from soils.