太湖中大银鱼_太湖新银鱼和寡齿新银鱼群体的遗传结构

利用ＲＡＰＤ技术对太湖中的大银鱼,太湖新银鱼,寡齿新银鱼的遗传结构进行了研究,共在太湖中的６个采样片采到大银鱼和太湖新银鱼样品,在两个采样片得到了寡齿新银鱼的样品。结果表明,太湖中不同水域的三种银鱼没有明显的遗传差异,三种银鱼中,大银鱼的遗传相似性指数显示最大,太湖新银鱼的次之,寡齿新银鱼的最小,说明大银鱼的遗传变异性最小,寡齿新银鱼的最大,太湖新银鱼的遗传变异性界两者之间。     

Zinc exposure has differential effects on uptake and metabolism of sulfur and nitrogen in Chinese cabbage

Zinc (Zn) is a plant nutrient; however, at elevated levels it rapidly becomes phytotoxic. In order to obtain insight into the physiological background of its toxicity, the impact of elevated Zn²⁺ concentrations (1 to 10 μM) in the root environment on physiological functioning of Chinese cabbage was studied. Exposure of Chinese cabbage (Brassica pekinensis) to elevated Zn²⁺ concentrations (≥ 5 μM) in the root environment resulted in leaf chlorosis and decreased biomass production. The Zn concentrations of the root and shoot increased with the Zn²⁺ concentration up to 68‐fold and 14‐fold, respectively, at 10 μM compared to the control. The concentrations of the other mineral nutrients of the shoot were hardly affected by elevated Zn²⁺ exposure, although in the root both the Cu and Fe concentrations were increased at ≥ 5 µM, whereas the Mn concentration was decreased and the Ca concentration strongly decreased at 10 µM Zn²⁺. The uptake and metabolism of sulfur and nitrogen were differentially affected at ≥ 5 µM Zn²⁺. Zn²⁺ exposure resulted in an increase of sulfate uptake and the activity of the sulfate transporters in the root, and in enhanced total sulfur concentration of the shoot, which could be ascribed partially to an accumulation of sulfate. Moreover, Zn²⁺ exposure resulted in an up to 6.5‐fold increase in water‐soluble non‐protein thiol (and cysteine) concentration of the root. However, nitrate uptake by the root and the nitrate and total nitrogen concentrations of the shoot were decreased upon Zn²⁺ exposure, demonstrating the absence of a mutual regulation of the uptake and metabolism of sulfur and nitrogen at toxic Zn levels. Evidently, elevated Zn²⁺ concentrations in the root environment did not only disturb the uptake, distribution and assimilation of sulfate, it also affected the uptake and metabolism of nitrate in Chinese cabbage.     

Interaction between protein, phytate, and microbial phytase. In vitro studies

The interaction between protein and phytate was investigated in vitro using proteins extracted from five common feedstuffs and from casein. The appearance of naturally present soluble protein-phytate complexes in the feedstuffs, the formation of complexes at different pHs, and the degradation of these complexes by pepsin and/or phytase were studied. Complexes of soluble proteins and phytate in the extracts appeared in small amounts only, with the possible exception of rice pollards. Most proteins dissolved almost completely at pH 2, but not after addition of phytate. Phytase prevented precipitation of protein with phytate. Pepsin could release protein from a precipitate, but the rate of release was increased by phytase. Protein was released faster from a protein-phytate complex when phytase was added, but phytase did not hydrolyze protein. Protein was released from the complex and degraded when both pepsin and phytase were added. It appears that protein-phytate complexes are mainly formed at low pH, as occurs in the stomach of animals. Phytase prevented the formation of the complexes and aided in dissolving them at a faster rate. This might positively affect protein digestibility in animals.     

Influence of storage practices on acrylamide formation during potato frying

A number of parameters linked to storage of potatoes were evaluated with regard to their potential to influence the acrylamide formation in French fries. Acrylamide, which is a potential human carcinogen, is reported to be formed during the frying of potatoes as a result of the reactions between asparagine and reducing sugars. This study was conducted using three potato varieties (Bintje, Ramos, and Saturna) typically used in Belgium, The Netherlands, and the northern part of France for French fry and crisp production. Saturna, mainly used in crisp production, appeared to be the least susceptible for acrylamide formation during frying. Especially storage at low temperatures (4 degrees C) compared to storage at 8 degrees C seemed to enhance acrylamide formation due to a strong increase in reducing sugars caused by low-temperature storage. Because of the reversible nature of this physiological reaction, it was possible to achieve a significant reduction of the reducing sugars after a reconditioning of the cold-stored potatoes for 3 weeks at 15 degrees C. All changes in acrylamide concentrations could mainly be explained by the reducing sugar content of the potato (R2 = 0.84, n = 160). This means that, by ensuring a low reducing sugar content of the potato tuber, the risk for acrylamide formation will largely be reduced. Finally the use of a sprout inhibitor did not influence the composition of the potato, and thus acrylamide formation was not susceptible to this treatment.     

Primary and secondary metabolite composition of kernels from three cultivars of Portuguese chestnut (Castanea sativa Mill.) at different stages of industrial transformation

Chestnut (Castanea sativa) is an important basic food in rural diets and a major starch crop used in a similar way to potatoes. Chestnuts are a fundamental economic resource in the "chestnut regions" not only for the fruit but also for the chestnut wood. Chestnuts have become increasingly important with respect to human health, for example, as an alternative gluten-free flour source. Chestnuts are also a rich source of other beneficial compounds, but there have been few studies on the composition during processing. In this study, we analyzed the chemical composition of three Portuguese cultivars at different stages of industrial processing. The chestnut cultivars were Longal, Judia, and Martaínha. All three cultivars had high moisture contents but were low in ash, crude fat, and crude protein contents, with high starch and low fiber contents. The free amino acid contents, including various essential amino acids, varied depending on the cultivar. All three cultivars also had a significant content of polyphenolics with gallic acid; ellagic acid was predominant among hydrolyzable and condensed tannins. Many of these compounds are known to exert significant positive effects on human health. The one-way analysis of variance for fresh chestnut shows significant differences among the three cultivars for most of the studied parameters. The same statistical analysis applied to each one of the two cultivars (Judia and Longal) sampled for the four processing steps analyzed indicates a significant effect of this factor in practically all of the constituents. On the other hand, the two-way analysis of variance shows that, besides the residual, the processing step and the interaction cultivar x processing step were the factors that more contributed for the total variation observed in the constituents analyzed, while the contribution of cultivar was much less significant.     

辣椒细胞质雄性不育系离体培养植株再生研究

以辣椒细胞质雄性不育系9704A和8214A为试材，取无菌苗真叶进行培养。研究了不同浓度的IAA,6-BA和GA3对不定芽的诱导和伸长作用，结果表明：MS+6-BA5.0 mg/L +IAA1.0 mg/L有利于不定芽的诱导。MB+IAA1.0 mg/ L +6-BA3.0 mg/L + GA32.0 mg/L +AgNO310.0 mg/L有利于芽的伸长。将2.0 cm以上的芽转入生根培养基诱导不定根发现：1/2Ms+IAA0.3~0.5 mg/L有利于不定根的诱导。     

优质稻核心种质青六矮1号及其衍生品种的性状相关性研究

对青六矮1号及其衍生品种(品系)的相关分析表明: 产量与有效穗数呈极显著正相关, 与每穗总粒数和每穗实粒数呈显著负相关; 倒三叶长与倒二叶长呈极显著正相关, 与着粒密度呈显著负相关, 与粒长呈显著正相关, 与垩白率呈显著负相关, 与垩白度呈极显著负相关, 与胶稠度呈显著正相关, 与直链淀粉含量呈显著负相关. 主成分分析表     

黄瓜根腐病致病病原的鉴定

在天津市及周边地区采集病株进行组织分离，同时分离到两类病原菌，经初步的生物学特性鉴定表明，引起黄瓜根腐病的主要病原菌为甜瓜疫霉、尖孢镰刀菌和茄病镰刀菌。经接种试验，认为甜瓜疫霉为主要致病菌。     

浓缩风能型风力发电机的整体模型风洞实验（第Ⅲ报）

低速风洞内进行的实验结果表明：当风速为５．５０ｍ／ｓ时，浓缩风能型风力发电机模型的输出功率为１００Ｗ，是直径相等的普通型风力发电机（风力机和发电机的总机械效率为０．３３２时）的输出功率的３．８倍；其输出功率相当于目前我国草原上使用的小型风力发电机输出功率的６倍；并且该机组的起动风速比相同叶轮普通型风力发电机模型的起动风速低     

In situ net N transformations in pine,fir, and oak stands of different ages on acid sandy soil, 3 years after liming

Summary The effect of liming on in-situ N transformations was studied in two stands of different ages of each of Scots pine (Pinus sylvestris L.), Douglas fir [Pseudotsuga menziesii (Mirb.) Franco], and common oak (Quercus robur L.). The stands were located on acid sandy soils in an area with high atmospheric N input. The organic matter of the upper 10-cm layer of the soil, including the forest floor, had a relatively high N content (C: N ratio <25) in all stands. Using a sequential core technique, N transformations were measured in both control plots and plots that had been limed 3 years previously with 3 t ha^-1 of dolomitic lime. Limed plots had a higher net NO _inf3 ^sup- production and a higher potential for NO _inf3 ^sup- leaching than the controls in all stands except that of the younger oak. Net N mineralization did not differ significantly between limed and control plots in oak stands and younger coniferous stands but was significantly lower in the limed plots of the older coniferous stands. It is concluded that long-term measurements of net N mineralization in limed forest soils are needed to evaluate the effect of liming with respect to the risk of groundwater pollution.