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91.
Serum leptin levels during the periparturient period in cows 总被引:1,自引:0,他引:1
Soliman M Ishioka K Yoshida R Komabayashi K Hatai H Matsui Y Hirai T Katagiri S Takahashi Y Kawakita Y Abe H Kitamura H Kimura K Saito M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1053-1056
Serum leptin concentrations were measured in antenatal and postnatal cows housed at two different locations. The mean serum leptin concentration was 9.2 +/- 0.6 ng/m l (n=22) in one group, and was slightly lower in the other (7.4 +/- 0.4 ng/ml, n=54), probably because of the different nutritional conditions between the two groups. There was no consistent variation in relation to the menstrual cycle and the periparturient period in both groups. Moreover, serum leptin concentrations during the periparturient period were independent of the number of delivery and the incidence of mastitis and milk fever. These results are quite different from those in rodents and human, suggesting the different regulatory mechanism of circulating leptin concentration in cows. 相似文献
92.
Kobayashi S Sato R Aoki T Omoe K Inanami O Hankanga C Yamada Y Tomizawa N Yasuda J Sasaki J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):429-435
Feline immunodeficiency virus (FIV) infection is characterized by chronic overactivation of immune and inflammatory system, resulting in anergic state and dysfunction of immune cells. Lactoferrin (LF), a glycoprotein present in exocrine secretions and neutrophils, plays an important role in host defense system. Our previous study showed that oral administration of bovine LF (bLF) suppressed oral inflammation, improved the clinical symptoms and decreased serum gamma-globulin as a marker of inflammation in FIV-infected cats with intractable stomatitis. The anti-inflammatory effect was partly involved in regulation of neutrophil function by bLF. In this study, to clarify the relationship between anti-inflammatory effects of bLF and peripheral blood mononuclear cells (PBMC), we examined the effect of bLF on proliferation, cell cycle progression and cytokine expression in mitogen-activated PBMC. MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide] assay showed that bLF inhibited the concanavalin A (ConA)-induced cell proliferation in FIV-infected cats with the asymptomatic carrier and AIDS-related complex (ARC) phase. Bovine LF restored ConA-induced cell cycle progression and resulted in suppression of the induced apoptosis in feline PBMC. Real-time RT-PCR showed that bLF suppressed ConA-induced expression of interferon-gamma and interleukin-2 in cells of the ARC group regardless of the time of its addition to the medium. These results suggest the hypothesis that therapy with bLF may have the potential to improve and protect functions of overactivated lymphocytes by modulating the cell proliferation, cell cycle and cytokines expression in cats in terminal stage of FIV infection. 相似文献
93.
Hitoshi Nakamura Shigeto Ohgake Norio Sahashi Nobuyuki Yoshikawa Takanori Kubono Tsuyoshi Takahashi 《Journal of Forest Research》1998,3(1):39-42
Seasonal variation of paulownia witches’-broom (PWB) phytoplasma within different organs (leaves, branch and trunk bark and
roots) in paulownia trees was investigated by the amplification of a PWB-specific DNA fragment by the polymerase chain reaction
(PCR). In leaf samples, PWB phytoplasma was first detected in June and the incidence gradually increased. On the other hand,
the PWB was detected at relatively low incidence in branch bark, trunk bark and roots and the incidence did not change among
seasons. A survey of PWB in 27 fields in the Tohoku district of Japan showed that malformed flower buds were observed in paulownia
trees in almost all of the fields. PWB-phytoplasma was also detected by PCR from paulownia trees in almost all of the fields
in Iwate and Fukushima Prefectures. The frequencies of trees in which phytoplasma was detected by PCR were higher than those
in which symptoms were observed. These results indicated that PCR amplification of a PWB-specific DNA fragment is an effective
tool for practical diagnose and that PWB is widely distributed in the Tohoku district of Japan. 相似文献
94.
Alginate-coated meristems from in vitro-grown strawberry (Fragaria × ananassa Duch.) were successfully cryopreserved following
dehydration by a vitrification solution. Excised meristems from cold-hardened plantlets at 4 °C for two weeks in the dark
were encapsulated into alginate-gel beads containing a mixture of 2 M glycerol plus 0.4 M sucrose. These encapsulated and
cryoprotected meristems were dehydrated with a highly concentrated vitrification solution (designated PVS2) for 2 hr at 0
°C prior to a plunge into liquid nitrogen. Successfully encapsulated vitrified meristems remained green and then developed
shoots within one week after plating without intermediary callus formation. The average rate of shoot formation of encapsulated
vitrified meristems amounted to nearly 90%. The cryogenic protocol was successfully applied to four cultivars of strawberry.
It was also confirmed that encapsulated vitrified meristems cooled to - 196 °C produced higher shoot formation than encapsulated
dried meristems. Besides, the recovery growth was much earlier than the latter. The encapsulation-vitrification method is
easy to handle and produces high levels of shoot formation. Thus, the protocol promises for cryopreservation of strawberry
germplasm.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
95.
Hirofumi Hirai Ryuichiro Kondo Kokki Sakai Yoshio Watanabe Ryuichiro Kurane 《Journal of Wood Science》1999,45(3):262-265
The reduction of ferric chelate caused by various wood-rot fungi was analyzed. Ferric chelate reductive activity was detected in cell-free extracts of seven wood-rot fungi:Phanerochaete chrysosporium, P. sordida YK-624,Ganoderma sp. YK-505,Coriolus versicolor, Bjerkandera adusta, Tyromyces palustris, andGloeophyllum trabeum. These fungi produced NADPH- or NADH-dependent ferric chelate reductive enzymes (or both) of different molecular weight. In the liquid culture ofP. sordida YK-624 andC. versicolor, a positive correlation was observed between extracellular MnP activity and intracellular NADPH-dependent ferric chelate reductive activity. 相似文献
96.
Tomoyoshi Yoshinaga Nobuyuki Tsutsumi Kathryn Ann Hall Kazuo Ogawa 《Fisheries Science》2009,75(5):1167-1176
In the mid-1990s, Neoheterobothrium hirame suddenly appeared as a new species in olive flounder Paralichthys olivaceus in Japanese coastal waters. Anemia caused by the parasite has prevailed in wild and cultured populations of olive flounder since that time. In this study, to clarify the origin of N. hirame, two Neoheterobothrium species, namely unidentified Neoheterobothrium species (tentatively abbreviated as Neoheterobothrium sp. PL) and N. affine, were collected from Paralichthys lethostigma and Paralichthys dentatus, respectively, off the east coast of North America and compared with N. hirame collected in Japan. No substantial differences were detected in the morphology and DNA sequences of ITS1-5.8S rRNA-ITS2 and mitochondrial cytochrome oxidase subunit I (mt COI) regions between N. hirame and Neoheterobothrium sp. PL. On the other hand, the congeneric N. affine was clearly distinguished from both N. hirame and Neoheterobothrium sp. PL in its longer isthmus and the DNA sequences in ITS1 and mt COI. The absence of differences between N. hirame and Neoheterobothrium sp. PL and the clear difference between both of these and N. affine indicate that N. hirame is conspecific with Neoheterobothrium sp. PL infecting P. lethostigma and that N. hirame was recently introduced from North America to Japan. 相似文献
97.
A cointegration analysis is conducted to examine the effect of fishery subsidies on fisheries production using data compiled
over more than 30 years in Japan. The results illustrate that one fishery production indicator (production value per fishermen)
shows a positive relationship with one particular group of government financial transfer (GFT) (that is, government general
service expenditures including cost for fishery managements, scientific researches, and other administrative activities).
No other tested results between GFTs and fishery indicators showed a real relationship. Although further scrutiny is awaited,
this study could provide an empirical basis for an argument that, under an effective fishing management system, fisheries
subsidies do not necessarily cause production increases or negative impact on fishing stocks. 相似文献
98.
Ikutaro Tsuyama Masahiro Horikawa Katsuhiro Nakao Tetsuya Matsui Yuji Kominami Nobuyuki Tanaka 《Journal of Forest Research》2012,17(2):137-148
The objective of this study was to identify climatic factors determining the distribution of a keystone understory taxon,
section Crassinodi of the genus Sasa, and assess the impacts of climate change on the taxon. Relationships between the distribution of sect. Crassinodi and five climatic variables were explored using classification tree analysis. Potential habitats under current climate and
future climate in 2081–2100 were predicted. Potential habitats were further divided into suitable and marginal habitats. The
predictive accuracy of the model was assessed using receiver operating characteristic analysis and by comparing model predictions
with an independent dataset. The model was reasonably accurate. It showed that the warmth index (WI) and snow cover were the
most important climatic variables for Crassinodi distribution. Potential habitats were limited to cooler regions with WI <102.7°C month. Suitable habitats were limited to
even cooler regions with WI <84.8°C month. The model also showed that areas with deeper snow than previously reported would
provide suitable habitats for Crassinodi under some climatic conditions. In 2081–2100, 37.4% of current potential habitats are predicted to become non-habitats because
of increases in WI. Most currently suitable habitats are predicted to vanish from western Japan by 2081–2100. Meanwhile, Hokkaido
and high-elevation areas of eastern Honshu will sustain suitable habitats. Sect. Crassinodi, which is adapted to less snowy climates, is predicted to be more affected by climate change than sect. Sasa and Macrochlamys, which are adapted to snowy climates. 相似文献
99.
Methane uptake and nitrous oxide emission in Japanese forest soils and their relationship to soil and vegetation types 总被引:1,自引:1,他引:1
Tomoaki MORISHITA Tadashi SAKATA Masamichi TAKAHASHI Shigehiro ISHIZUKA Takeo MIZOGUCHI Yoshiyuki INAGAKI Kazuhiko TERAZAWA Satoshi SAWATA Masanori IGARASHI Hiroshi YASUDA Yasuhiro KOYAMA Yoshihito SUZUKI Nobuyuki TOYOTA Masamichi MURO Masaru KINJO Hirokazu YAMAMOTO Daitaro ASHIYA Yoichi KANAZAWA Tetsu HASHIMOTO Hidetaka UMATA 《Soil Science and Plant Nutrition》2007,53(5):678-691
100.