全文获取类型
收费全文 | 207篇 |
免费 | 3篇 |
国内免费 | 2篇 |
专业分类
林业 | 21篇 |
农学 | 4篇 |
基础科学 | 2篇 |
10篇 | |
综合类 | 10篇 |
农作物 | 2篇 |
水产渔业 | 19篇 |
畜牧兽医 | 143篇 |
园艺 | 1篇 |
出版年
2022年 | 2篇 |
2021年 | 7篇 |
2020年 | 1篇 |
2019年 | 6篇 |
2018年 | 4篇 |
2017年 | 1篇 |
2016年 | 7篇 |
2015年 | 7篇 |
2014年 | 11篇 |
2013年 | 14篇 |
2012年 | 7篇 |
2011年 | 16篇 |
2010年 | 1篇 |
2009年 | 4篇 |
2008年 | 14篇 |
2007年 | 14篇 |
2006年 | 15篇 |
2005年 | 26篇 |
2004年 | 18篇 |
2003年 | 15篇 |
2002年 | 13篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1995年 | 1篇 |
1990年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有212条查询结果,搜索用时 455 毫秒
71.
72.
73.
ABSTRACT: This study documents the genetic population relationships between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus by means of the microsatellite technique with an emphasis on the extent of genetic difference between Ezo- and Kuro-awabi populations. Eight markers were employed to screen five populations each collected from Ezo- and Kuro-awabi habitats. All eight loci showed polymorphisms in all populations (number of alleles per locus, 11.1–14.1; averaged expected heterozygosity, 0.64–0.70). The number of loci that yielded significant genetic heterogeneities (allele frequency distribution and/or pairwise θ ) between Ezo- and Kuro-awabi populations was larger than that generated in comparisons between populations within Ezo- and Kuro-awabi habitats. According to the bootstrap neighbor-joining trees constructed on the basis of two genetic distance measures ( D A and D ST ), the 10 populations fell into two clusters of populations (Ezo-awabi and Kuro-awabi habitat groups), and the results of hierarchical amova testing also supported the neighbor-joining clustering. The outcomes presented here suggest that the microsatellite markers used in this study are potentially an efficient means to differentiate Ezo- and Kuro-awabi populations. 相似文献
74.
75.
Sakai H Horiguchi N Endoh D Nakayama K Hayashi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):299-304
In the present study, we examined effects of radiofrequency (RF) radiation at 40 kHz on hepatic injury in Long-Evans Cinnamon (LEC) rats, an animal model for human Wilson disease, which is a heritable disease of copper metabolism in the liver. The activities of ALT and AST in serum of LEC rats exposed to RF radiation for 2 weeks were approximately 3.8-fold and 2-fold higher than those in serum of sham-exposed rats, respectively. Although there were no significant differences in hepatic copper contents between LEC rats exposed to RF radiation for 2 weeks and sham-exposed rats, copper contents in the kidney and serum of exposed LEC rats were approximately 4.2-fold and 12.9-fold higher than those in sham-exposed rats, respectively. Relative O??-scavenging activities in the S-100 fraction of the liver of LEC rats exposed to RF radiation for 2 weeks were 1.6-fold higher than those in sham-exposed rats. No significant differences were observed in activities of AST and ALT in serum and relative O??-scavenging activity in the S-100 fraction of the liver of normal control WKAH rats that were sham-exposed and exposed to RF radiation. No significant differences were observed in copper contents in the liver, kidney and serum of WKAH rats that were sham-exposed and exposed to RF radiation for 2 weeks. The results show that RF radiation at 40 kHz induced hepatic injury in LEC rats. 相似文献
76.
Viet Linh N Kikuchi K Nakai M Noguchi J Kaneko H Dang-Nguyen TQ Maedomari N Nguyen BX Nagai T Manabe N 《The Journal of reproduction and development》2011,57(5):620-626
We have shown in pigs that oocytes denuded of cumulus cells at 24 h of in vitro maturation culture and subsequently matured for a total of 46 h (DO24 oocytes) have lower cytoplasmic maturity than those matured with cumulus cells for 46 h and then denuded (DO46 oocytes). In the present study, DO24 zona-free oocytes were fused with one (1C) or two (2C) cytoplasmic fragments produced by serial centrifugation ("centri-fusion") of DO46 oocytes (DO24+1C and DO24+2C oocytes, respectively). Groups of (1) DO46 (a control), (2) DO24, (3) DO24+1C and (4) DO24+2C oocytes were partheno-activated by an electrical pulse or fertilized in vitro and subsequently cultured for 6 days. In the fused groups, female pronucleus (FPN) formation rates were higher than that in the DO24 group after parthenogenetic activation (PA); however, the blastocyst rates were intermediate between those of the control and DO24 groups. After in vitro fertilization, the male pronucleus (MPN) formation rates in the fused groups were similar to that in the control group and higher than that in the DO24 group; the normal fertilization rate in the DO24+2C group was higher than that in the DO24 group and similar to that in the control group, resulting in significantly higher blastocyst rates in the DO24+2C and control groups than that in the DO24 group. These results suggest that centri-fusion using ooplasm from fully matured DO46 oocytes can offer a potentially novel approach for restoration of cytoplasmic maturity to oocytes with low developmental ability and subsequent improvement of fertilization and developmental competence. 相似文献
77.
Nakao S Tanaka R Hamabe L Suzuki S Hsu HC Fukushima R Machida N 《Journal of Feline Medicine and Surgery》2011,13(6):463-466
An 11-month-old, 3 kg, female domestic shorthair cat was referred to evaluate cardiac structure and function. Echocardiography revealed the membrane dividing the left atrium into two chambers, a large defect in the lower part of the atrial septum, and turbulent blood flow from the distal left atrium into the right atrium. These findings suggested cor triatriatum sinister (CTS) with incomplete atrioventricular septal defect (AVSD). The cat was treated with medications for management of congestive heart failure. In the end, she died from right-sided heart failure 17 months after the initial presentation. At necropsy, a fibromuscular membrane with a round orifice in the left atrium and an ostium primum defect were confirmed, and the definitive diagnosis of CTS with incomplete AVSD was made. To our knowledge, this study presents the first case report of CTS with incomplete AVSD in a cat. 相似文献
78.
79.
Rieko OKAME Keiko NAKAHARA Yumiko KATO Makoto BANNAI Noboru MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):661-668
It has been suspected that in comparison with glucose or fatty acids, the levels of amino
acids may readily change with different forms of exercise. In the present study, we
measured the concentrations of amino acids, glucose, triglycerides, total protein and
total cholesterol in the blood and/or cerebrospinal fluid (CSF) of rats subjected to
forced running exercise on a treadmill, and voluntary running exercise using a wheel, with
a constant running distance of 440 m. Rats that performed no running and rats subjected to
immobilization stress were used as controls. We observed a few significant changes in the
levels of plasma glucose, triglycerides, total protein and total cholesterol in all
groups. Whereas, plasma amino acid levels were significantly changed by exercise and
stress, especially during the light period. The plasma levels of many amino acids were
specifically increased by forced running; some were decreased by immobilization stress.
Few amino acids showed similar changes in their levels as a result of voluntary running.
In addition, there was a significant difference in the degree of amino acid imbalance
between blood and CSF. These results provide the first information on changes in levels of
amino acids in plasma and CSF resulting from forced and voluntary exercises. 相似文献
80.
Noboru KUDO Natsumi ISHIKAWA Aki YAMANE Hiromi IKADAI Takashi OYAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):113-116
Gongylonema pulchrum is an important parasite of captive
primates. Twelve rabbits were infected with 30 third-stage larvae of G.
pulchrum. At 4–7 months post-infection, animals were administered levamisole at
a single dose of 12 mg/kg, levamisole at 8 mg/kg three times at 2-day intervals,
levamisole at a single dose of 8 mg/kg after administration of mebendazole at 70 mg/kg for
3 days or 8 ml of distilled water for 3 days (control). Necropsy at 14
days after treatment revealed that single and multiple dosages of levamisole reduced
nematode burdens by 68.4% and 89.5%, respectively. The combined regimen of mebendazole and
levamisole exhibited high efficacy for treating G. pulchrum located
widely within the upper digestive tract, with a reduction of 98.2%. These results suggest
that this combined chemotherapy treatment may be effective against G.
pulchrum infection, including buccal and lingual gongylonemiasis in
primates. 相似文献