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71.
Kemmochi S Fujimoto H Woo GH Hirose M Nishikawa A Mitsumori K Shibutani M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(5):655-664
We have shown phosphoinositide 3-kinase (PI3K)/Akt signaling activation in thyroid capsular invasive carcinomas (CICs), which are highly induced by promotion with sulfadimethoxine (SDM) in a rat 2-stage thyroid carcinogenesis model. To examine the potency of calcitriol, a synthetic vitamin D3 analog, on the development or progression of CICs, male F344 rats were injected with calcitriol (0.1 μg/kg body weight) three times a week intraperitoneally, during an entire period of SDM-promotion for 13 weeks (Experiment 1) or during the last 2 weeks of a 15-week SDM-promotion (Experiment 2). Initiation with N-bis(2-hydroxypropyl)nitrosamine preceded all treatments. In Experiment 1, long-term calcitriol treatment reduced the multiplicity of CICs, while cell proliferation activity, estimated by Ki-67 cell index in the induced CICs, was unchanged with SDM-promotion alone. Considering the strong dependency of promotion with SDM during the early stages on thyroid-stimulating hormone, the reduced multiplicity in Experiment 1 may be due to the effect on an early stage of neoplastic proliferation. Although the magnitude was mild, cell proliferation activity was decreased in existing CICs after short-term calcitriol treatment in Experiment 2, which was associated with a mild decrease in cyclin-dependent kinase-2-positive cells, cytoplasmic immunolocalization of phosphorylated, inactive, Rb protein and a mild increase in nucleocytoplasmic expression of p27(kip1). Although the effect was mild at the late stage of SDM-promotion in this hypothyroidism-related thyroid carcinogenesis model, our results suggest that calcitriol targets cell proliferation via inhibition of a molecular cascade downstream of PI3K/Akt signaling, controlling G1/S transition. 相似文献
72.
Said AW Kodani M Usui T Fujimoto Y Ito T Yamaguchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(4):545-548
Embryonated chicken eggs (ECEs) are routinely used to isolate equine influenza virus. Propagation of the virus in ECEs results in selection of variants. In the present study, we determined nucleotide sequences of entire coding regions of parent A/equine/Tottori/1/07 (H3N8) and its derivatives that have different passage histories in ECE. After 12 passages, nucleotide sequence analysis predicted 3 amino acid substitutions in hemagglutinin (HA; 2 in HA1 and 1 in HA2). The two amino acid substitutions in HA1 were located in the vicinity of the cell receptor-binding site. Three other amino acid substitutions were predicted in internal proteins, 1 in the M1, 1 in the NP and 1 in the PA. This is the first report showing mutations in the internal protein genes of equine influenza virus associated with adaptation to ECE. 相似文献
73.
74.
Tamie Noda Koichi Murakami Tetsuo Asai Yoshiki Etoh Tomoe Ishihara Toshiro Kuroki Kazumi Horikawa Shuji Fujimoto 《Acta veterinaria Scandinavica》2011,53(1):38
Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in the dominant lineage before and after this event remain unknown. This study determined S. Enteritidis lineages before and after this pandemic event in Japan using multilocus sequence typing (MLST). Thirty S. Enteritidis strains were collected in Japan between 1973 and 2004, consisting of 27 human strains from individual episodes, a bovine strain, a liquid egg strain and an eggshell strain. Strains showed nine phage types and 17 pulsed-field profiles with pulsed-field gel electrophoresis. All strains had homologous type 11 sequences without any nucleotide differences in seven housekeeping genes. These MLST results suggest that S. Enteritidis with the diversities revealed by phage typing and pulsed-field profiling has a highly clonal population. Although type 11 S. Enteritidis may exhibit both pleiotropic surface structure and pulsed-field type variation, it is likely to be a stable lineage derived from an ancestor before the 1980s and/or 1990s pandemic in Japan. 相似文献
75.
D. R. Lynch L. M. Kawchuk C. A. Schaupmeyer J. Holley D. K. Fujimoto D. Driedger T. R. Tarn D. Waterer J. Wahab M. S. Goettel 《American Journal of Potato Research》2001,78(5):339-343
AC Maple Gold is a high-yielding oval/oblong, yellow-fleshed potato with an attractive appearance for the fresh market coupled with excellent french fry quality. The cultivar can be harvested early for french fry processing, but also maintains excellent fry quality during long-term storage. AC Maple Gold produces french fries with a uniform deep yellow color. 相似文献
76.
D. R. Lynch M. S. Goettel L. M. Kawchuk D. K. Fujimoto S. J. Peloquin C. A. Schaupmeyer J. Holley D. Driedger D. Waterer J. Wahab 《American Journal of Potato Research》2001,78(5):327-332
AC Glacier Chip has a maincrop maturity, higher yields than Snowden and excellent quality (color and dry matter) for the chip industry. The tubers are oval and very uniform in size with white flesh. It performs best under irrigated conditions. AC Glacier Chip provides the industry with a high-yielding chip cultivar that produces excellent quality fried product out of the field and from long-term storage. 相似文献
77.
Luis Raul Tovar E. Brito T. Takahashi T. Miyazawa J. Soriano K. Fujimoto 《Plant foods for human nutrition (Dordrecht, Netherlands)》1989,39(4):299-309
Amaranth was a major crop among the Aztecs. In Mexico the seed is popped and eaten with brown sugar. The crude protein content of the seed is 14±2% but its contents of lysine and tryptophan are 6.2 and 1.6 g/16 g N respectively. We developed a popping method based on a fluid bed system (FBS) whereas the traditional method (TM) is just to pop the seeds manually in a hot plate. Assays carried out were evaluation of racemization of the amaranth protein due to heat treatment, amino acid composition of the raw and heat treated seeds and a biological experiment testing whether leucine was the most limiting amino acid of amaranth protein. Male rats were fed both popped amaranths and roasted amaranth. Parboiled amaranth and casein were controls. The results were: (a) Lys, Arg and Cys were damaged in the heat treated seeds; (b) Asp, Met, Glu, Ala and Phe were racemized in that decreasing order in the seeds popped and roasted by the TM; (c) the estimated net protein retention (NPR) and estimated net protein utilization (NPU) of popped amaranths by either method were not different, but were lower than for the parboiled amaranth. The parboiled amaranth was not different from casein; (d) Leu was not the most limiting amino acid in any of the amaranth seeds tested. After Lys, sulfur amino acids appear to be the next most limiting in severely heat treated amaranth. The FBS seems to be a promising method for popping amaranth at industrial level. 相似文献
78.
水旱轮作大豆的营养生长与根系活力的关系 总被引:1,自引:0,他引:1
大豆是实行水旱轮作制度的重要作物之一,水田的排水不良是导致轮作大豆减产的重要原因.通过对水旱轮作大豆幼苗进行过湿处理,分析了不同大豆品种的营养生长特性与根系生理活性的关系.轮作大豆在过湿条件下,所有品种的生育都受到抑制,干物质生产受抑制程度存在着品种间的差异.营养生长诸性状中,伤流速度受过湿处理影响最大,处理区的伤流速度仅为对照区的64.2%.过湿处理导致伤流液中氮含量几乎减少了一半,伤流中的氮含量与叶片氮素含量之间存在极显著正相关关系,过湿条件下叶片氮含量的减少是由于根的氮素吸收能力的低下所致.遭遇过湿逆境时的植株个体大小与维持物质生产关系密切.过湿处理期间及其后的干物质增加量的大小与以伤流速度为代表的根系活力密切相关,说明从田间管理技术上提高大豆的根系活力以维持水旱轮作大豆的干物质生产是可行的. 相似文献
79.
Rivero JC Inoue Y Murakami N Horii Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):519-521
The effects of host age and sex on susceptibility to Strongyloides venezuelensis in Wistar rats were examined by counting larvae recovered from the lungs of animals 3 days after infection. The susceptibility of female rats to S. venezuelensis rapidly decreased with age and elevated estrogen. Resistance in female rats inoculated at 6 and 10 weeks of age was nine and twenty-fold higher, respectively than that in the youngest group (3 weeks). In contrast, the susceptibility of male animals was lowest in the youngest group, then increased with age and elevated testosterone. Sex differences in susceptibility were not evident in the youngest group, but became apparent with age. 相似文献
80.
Imanishi S Sugimoto M Morita M Kume S Manabe N 《The Journal of reproduction and development》2007,53(5):1131-1136
The mRNA expression of GPRC5B, an orphan G protein-coupled receptor, is induced by retinoic acid (RA). Because RA plays critical roles in embryonic development, reproductive functions, metabolism and homeostasis, GPRC5B is also considered crucial in these physiological events. We investigated the changes in expression of GPRC5B and RA receptor (RAR) alpha mRNAs and immunohistochemical localization of their proteins in the murine placenta and yolk sac at 13.5, 15.5 and 17.5 days post coitus. Stable levels of GPRC5B and RARalpha mRNAs were detected in the placenta and yolk sac. In the placenta, GPRC5B was present in maternal and fetal vascular endothelial cells, stromal cells, fibroblast-like cells and glycogen cells. A strong reaction to RARalpha was detected in maternal and fetal vascular endothelial cells and stromal cells. The levels of GPRC5B and RARalpha proteins in maternal and fetal vascular endothelial cells decreased with gestation. In the yolk sac, GPRC5B and RARalpha proteins were detected in vascular endothelial cells, but their levels did not change during the gestation period. These findings indicate that GPRC5B is involved in RA-dependent morphogenesis/angiogenesis and regulation of extracellular matrix synthesis in the murine placenta and yolk sac. 相似文献