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51.
Recombinant bovine interferon-gamma enhances expression of class I and class II bovine lymphocyte antigens 总被引:2,自引:0,他引:2
F Walrand F Picard K McCullough S Martinod D Lévy 《Veterinary immunology and immunopathology》1989,22(4):379-383
Recombinant bovine interferon-gamma augments expression of class I and class II histocompatibility antigens on the surface membrane of bovine lymphocytes. Immunofluorescence techniques using a series of monoclonal anti-HLA antibodies demonstrate that this enhancement is detectable as early as 24 h after incubation with rBoIFN, while maximum surface expression is obtained within 3-5 days. A concentration as low as 10 units of rBoIFN is effective. Such results may be useful for characterizing the BoLA gene products. 相似文献
52.
53.
Granger N de Fornel P Devauchelle P Segond S Delisle F Rosenberg D 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2005,19(1):23-28
It is difficult to predict the size of pituitary corticotroph tumors in dogs with Cushing's disease (pituitary-dependent hyperadrenocorticism [PDH]) without pituitary imaging techniques. The purpose of this study was to examine the relationship between plasma adrenocorticotropin hormone (ACTH) precursor concentration and pituitary size in dogs with Cushing's disease. Plasma concentrations of ACTH precursors (pro-opiomelanocortin [POMC]/pro-ACTH) and pituitary tumor height/brain area were measured in 36 dogs with pituitary corticotroph adenomas of various sizes. There was a correlation between tumor size (measured as the pituitary tumor height/brain area ratio [P/B]) and POMC/pro-ACTH concentration (r = .70; P < .0001). Dogs with P/B > or = 0.40 x 10(-2) mm(-1) had higher concentrations of ACTH precursors than dogs with P/B < 0.40 x 10(-2) mm(-1) (median concentration 85 pmol/L, range 15-1,350 pmol/L, n = 14 versus 15 pmol/L, range 15-108 pmol/L, n = 22; P < .0001). With a threshold of 35 pmol/L of POMC/pro-ACTH concentration, the estimated sensitivity and specificity of the kit were 93% (95% confidence interval [CI], 79-100%) and 86% (95% CI, 73-100%), respectively. We interpret these data as indicating that measurement of POMC and pro-ACTH might be of value in the characterization of tumor size in dogs with Cushing's disease. Low POMC/pro-ACTH concentrations make it unlikely that a large pituitary tumor exists in dogs with PDH. 相似文献
54.
1. Broiler breeder females were fed restricted allocations of a standard wheat-soy ration to meet target body weights. They were housed on raised plastic slotted floors (S) or wood shavings litter (L) from hatch to 8 weeks when each pen of 12 birds was transferred to another pen in the same block in a 2 x 2 factorial experiment (LL, LS, SL and SS). Measures of bird welfare were taken at 4 weeks of age, and at 9 and 10 weeks following transfer to the new pens. 2. At 4 weeks of age, birds reared on S spent more time standing, pecking the feeder, the wall and other birds and less time pecking the floor compared with those on L. Gentle feather pecks, strong feather pecks and strong feather pulls were more common in S than L. 3. Mean body weight was higher and coefficient of variation lower in birds on L than S at the end of the experiment. Feather loss and damage scores at 10 weeks were higher for birds reared from hatch to 8 weeks on S. 4. Birds that were reared on S continued to peck more at the pen walls after transfer to new pens but there was no other carry-over effect on behaviour. Birds on S at 9 and 10 weeks pecked more at the walls and less often at the floor, and rested less often. There was more feather pecking on S than on L in the second week post transfer. 5. Tonic immobility was greater and plasma corticosterone concentrations were lower at the end of the experiment in birds on L than S at 9 and 10 weeks of age. The heterophil-lymphocyte ratio was similar between treatments at 4 weeks and after the birds were moved to a new pen. 6. The results are consistent with the view that litter and wall pecking has de-arousing properties and that this activity is re-directed foraging that diminishes the stress of feed restriction. 相似文献
55.
OBJECTIVE: To evaluate using strain gauges, a hoof cast with heel wedge, and a therapeutic shoe with unsupported toe for their effectiveness in redistribution of load from the dorsal hoof wall. STUDY DESIGN: In vitro biomechanical study. SAMPLE POPULATION: Twenty forelimb specimens. METHODS: Rosette strain gauges were placed on the dorsal and lateral hoof wall of 20 normal shaped hooves. Limbs were loaded vertically using a tensile testing machine with a 1 Hz sinusoidally cycling load up to 3000 N during 15 seconds. Mean values of principal strain and direction at 2500 N load were calculated for 3 experimental conditions (unshod, therapeutic shoe with unsupported toe, and hoof cast with heel elevation) and tested by ANOVA (P<.05). RESULTS: Vertical limb loading in an unshod hoof leads to a biaxial compression of the dorsal wall with high longitudinal compression (epsilon2 = -1515 microm/m). Principal strain at the dorsal wall (epsilon2) was decreased by 23% with the therapeutic shoe and by 59% with the hoof cast. On the lateral hoof wall principal strain was unchanged with the shoe, but increased by 34% with the cast. CONCLUSIONS: Strain measurements indicate unloading of the dorsal hoof wall by both methods with the cast being more effective than the shoe. CLINICAL RELEVANCE: The hoof cast with wedge offers substantial unloading of the dorsal wall, but increases load on the quarter. Therefore a hoof cast would likely be most helpful in acute laminitis when palmar structures can still bear load. The therapeutic shoe offers rehabilitation and regrowth of the dorsal wall without increased load on the quarter wall. 相似文献
56.
Summary Three hundred Warren Isabrown female day old chicks were raised for 18 weeks in 3 distinct artificial climates described as temperate (20°C, 60% RH), hot dry (32°C, 40% RH) and hot humid (32°C, 90% RH). At 18 weeks each group was divided into 3 and re-allocated to 3 similar air-conditioned laying rooms as during the growing period. Different diets were fed to the 3 groups up to 18 weeks. The energy needed in order to produce the same average live body weight at 18 weeks was similar in all the 3 climatic chambers. The effect of treatments in the growing period was not significantly different (P>0·05) for most of the parameters subsequently measured during the laying period. The exception was the body weight gain between 18 and 38 weeks and the food and energy intakes. However, apart from the sexual maturity, all these parameters were negatively influenced (P<0·05) by the effect of hot climates during the laying period irrespective of method of rearing. The results of this study seem to indicate that ambient temperature experienced during the first 18 weeks after hatching has little effect on subsequent productivity and that rearing birds at high ambient temperature does not acclimatise them to these conditions better than rearing them under temperate conditions.
Adaptacion Climatica De Gallinas Ponedoras
Resumen Se leavantaron 300 pollitas de un día de nacidas, Warren Isabrown, por 18 semanas en 3 climas diferentes; templado (20°C, 60% HR), seco caliente (32°C, 40% HR) y caliente húmedo (32°C, 90% HR). A las 18 semanas cada grupo fue dividido en 3 y re-instalado en 3 cuartos de postura con aire acondicionado durante el período de crecimiento. Los 3 grupos recibieron diferentes dietas hasta las 18 semanas. La energía necesaria para producir el mismo peso promedio a las 18 semanas fue el mismo en las tres cámaras climáticas. El efecto del tratamiento durante el período de crecimiento no fue significativamente diferente (P>0·05) para la mayoría de parámetros medidos subsecuentemente durante el peródo de postura. La excepción fue el aumento en peso corporal entre las 18 y 38 semanas y la energía consumida. Sin embargo, aparte de la madurez sexual, todos los parámetros fueron afectados negativamente (P<0·05) por el efecto de los climas cálidos durante el período de postura, irrespectivamente del manejo. Los resultados de este estudio parecen indicar, que la temperatura ambiente experimentada durante las primeras 18 semanas después del nacimiento, tuvo poco efecto sobre la productividad y que la crianza de aves a temperatura ambiente alta, no las aclimata para esas condiciones mejor que levantandolas bajo un clima templado.
Adaptation Climatique De La Poule Pondeuse
Resumé Trois cents poussins femelles d'un jour de souche Warren Isabrown ont été élevés jusqu'à l'âge de 18 semaines dans 3 climats artificiels distincts, respectivement tempéré (20°C, 60% HR), chaud sec (32°C, 40% HR) et chaud humide (32°C, 90% HR). A l'âge de 18 semaines chaque lot d'oiseaux a été divisé en trois et réparti à nouveau en 3 cellules de ponte conditionnées de la même façon que les salles de croissance. Différentes rations alimentaires sont nourries aux oiseaux jusqu'à l'âge de 18 semaines. La consommation d'énergie nécessaire pour produire le même poids vif à 18 semaines a été sensiblement égale dans les 3 climats. L'effet des traitements en période de croissance n'a pas été significativement différent (P>0·05) sur la presque totalité des paramètres étudiés en ponte, exception faite du gain de poids vif 18–38 semaines, des ingérés alimentaires et énérgétiques. Par contre, à l'exception de la maturité sexuelle, tous ces paramètres ont à chaque fois été influencés négativement (P<0·05) par les effets des climats chauds en période de ponte, indépendamment du climat d'élevage. Les résultats de cette expérience semblent indiquer que la température expérimentée pendant les 18 premières semaines après l'éclosion a eu peu d'effet sur la productivité ultérieure, et que l'élevage des oiseaux à hautes températures ambiantes ne leur confère pas une meilleure acclimatation à ces mêmes conditions que lorsqu'ils sont élevés en milieux tempérés.相似文献
57.
Borenstein N Behr L Chetboul V Tessier D Nicole A Jacquet J Carlos C Retortillo J Fayolle P Pouchelon JL Daniel P Laborde F 《Veterinary surgery : VS》2004,33(4):309-313
OBJECTIVE: To report a technique for minimally invasive occlusion of patent ductus arteriosus (PDA) and outcome in 5 dogs. STUDY DESIGN: Clinical cases. Animals: Five, 4-6-month-old, dogs with PDA. MATERIALS AND METHODS: Titanium ligating clips were used for PDA closure in all dogs. Three dogs had video-enhanced mini-thoracotomy PDA occlusion. Two other dogs had thoracoscopic PDA occlusion using a custom-designed thoracoscopy clip applicator. RESULTS: Thoracoscopic PDA occlusion was successful in both dogs in which it was attempted. Complete PDA closure was achieved in 4 dogs. Three months after surgery, the largest dog had residual ductal flow that hemodynamically was insignificant. CONCLUSIONS: Although technically demanding, minimally invasive PDA occlusion is a safe and reliable technique in dogs. Preoperative measurement of the diameter of the PDA is crucial to determine if complete closure with metal clips can be achieved. CLINICAL RELEVANCE: Minimally invasive PDA occlusion should be considered as an alternative to occlusion via conventional thoracotomy. 相似文献
58.
Bibring JP Langevin Y Mustard JF Poulet F Arvidson R Gendrin A Gondet B Mangold N Pinet P Forget F Berthé M Bibring JP Gendrin A Gomez C Gondet B Jouglet D Poulet F Soufflot A Vincendon M Combes M Drossart P Encrenaz T Fouchet T Merchiorri R Belluci G Altieri F Formisano V Capaccioni F Cerroni P Coradini A Fonti S Korablev O Kottsov V Ignatiev N Moroz V Titov D Zasova L Loiseau D Mangold N Pinet P Douté S Schmitt B Sotin C Hauber E Hoffmann H Jaumann R Keller U Arvidson R Mustard JF Duxbury T 《Science (New York, N.Y.)》2006,312(5772):400-404
Global mineralogical mapping of Mars by the Observatoire pour la Mineralogie, l'Eau, les Glaces et l'Activité (OMEGA) instrument on the European Space Agency's Mars Express spacecraft provides new information on Mars' geological and climatic history. Phyllosilicates formed by aqueous alteration very early in the planet's history (the "phyllocian" era) are found in the oldest terrains; sulfates were formed in a second era (the "theiikian" era) in an acidic environment. Beginning about 3.5 billion years ago, the last era (the "siderikian") is dominated by the formation of anhydrous ferric oxides in a slow superficial weathering, without liquid water playing a major role across the planet. 相似文献
59.
Filippi JJ Lanfranchi DA Prado S Baldovini N Meierhenrich UJ 《Journal of agricultural and food chemistry》2006,54(17):6308-6313
The essential oil of Achillea ligustica from Corsica was investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 82 compounds representing 94.0% of the oil were tentatively identified. The main constituents were the camphane derivatives, representing >30% (camphor, 21.3%; borneol, 6.2%; bornyl acetate, 3.5%) of the whole oil, and santolina alcohol (19.3%). The enantiomeric distribution of 8 chiral constituents was determined by GC-MS using two enantioselective stationary phases (DIME-beta-CD and Lipodex-E). Racemic santolina alcohol, required for optimization of the enantioselective GC conditions, was prepared by an original two-step synthesis from 2,5-dimethylhexa-2,4-diene. The whole essential oil was tested for its antibacterial activity against a wide range of bacteria using a paper disk method. The results show a promising activity against Streptomyces species. 相似文献
60.
Ernst NS Freeman DE Mackay RJ 《Journal of the American Veterinary Medical Association》2006,229(12):1945-1948
CASE DESCRIPTION: A 6-year-old Appaloosa mare was examined because of inappetance, difficulty eating, and swelling and mucopurulent discharge in the right eye. CLINICAL FINDINGS: Results of a CBC and serum bio-chemical analysis revealed no important findings. Ophthalmologic examination revealed scarring and ulceration of the superficial layers of the cornea. Endoscopic examination of the upper portion of the respiratory tract and auditory tube diverticula (guttural pouches) revealed abnormal thickness of the right stylohyoid bone and a plaque suggestive of mycotic growth on the left internal carotid artery. Radiographic examination revealed right-sided otitis media. Temporohyoid osteoarthropathy in the right guttural pouch and mycosis in the left guttural pouch were diagnosed. TREATMENT AND OUTCOME: Ceratohyoidectomy of the right stylohyoid bone was performed, and the left internal carotid artery was occluded via placement of stainless steel spring embolization coils. The mare regained the ability to eat without difficulty and improved clinically for approximately 4 weeks. However, the mare returned to the medical center 53 days after surgery with left-sided Horner syndrome, atrophy of the right side of the tongue, and a 3-week history of dysphagia and weight loss. Endoscopic evaluation revealed progression of mycotic growth in the left guttural pouch. The mare was euthanatized. CLINICAL RELEVANCE: Although the mycotic lesion in the left guttural pouch was an incidental finding at the time of initial examination, the lesion progressed to cause dysphagia and Horner syndrome after occlusion of the left internal carotid artery, a treatment that is typically associated with resolution of guttural pouch mycosis. Arterial occlusion is not necessarily a reliable method of resolving guttural pouch mycosis. 相似文献