无锡市锡山区失地农民再就业的影响因素

失地农民的再就业问题关系到农民失地后的生活保障和生活出路，长期以来一直是各级政府和理论界所关注的重点问题。在走访调查无锡市锡山区东港镇X社区的失地农民再就业情况的基础上，从政府、劳动力因素和社会因素3个方面探讨分析了无锡市锡山区失地农民再就业的影响因素。      

棉花花铃期持续高温对不同部位成铃和品质的影响

为了探讨花铃期高温对棉花成铃的影响,设置了6个品种试验,调查生长发育、农艺性状与纤维品质。结果表明花铃期高温天气出现频率高,持续时间长,棉花下部棉铃,尤其是中部优质棉铃的成铃率下降,对纤维品质影响大,高温期开花5-6果枝成铃的纤维长度变短,中下部1-6果枝铃的纤维断裂比强度明显低于上部7-9果枝。7月中下旬应增施磷钾肥,提高滴灌频率,缩短滴灌时间,改善田间小气候,减轻天气对棉花产量和品质的影响。     

玉米DDGS中抗生素残留的潜在风险及其检测方法

本文就DDGS产品中抗生素残留风险及其检测方法作一综述。     

Variation of Color and Ray Pattern in Juvenile Shells in Hatchery‐produced Freshwater Triangle Pearl Mussels,Hyriopsis cumingii,in China

The variation and inheritance of juvenile shell color and pigmentation pattern in the freshwater mussel, Hyriopsis cumingii, were reported in 1‐yr‐old progeny of eight families. There were three distinctive phenotypes of shell color and pigmentation patterns observed, including a greenish‐brown shell with radial rays, yellowish‐brown shell with radial rays, and yellowish‐brown shell without radial rays. There were no greenish‐brown individuals without radial rays. The shell color phenotypes showed variation with the growth in juvenile Stage I (1–3 cm in shell length), and the percentage of individuals with radial rays increased once they reached a shell length of 11 mm and then stabilized after reaching 20 mm in shell length. Shell color differentiation became more apparent at a shell length of 26 mm. Results of chi‐square tests of the segregation ratio of shell color or ray phenotypes obtained from eight families at juvenile Stage II (6–9 cm in shell length) suggested that greenish‐brown is controlled by a dominant allele (G) and yellowish‐brown‐shell phenotype is by a recessive allele (y); the ray pattern phenotype is controlled by a recessive (r) and a dominant allele (R) at a single locus. Shell color phenotypes may be a useful genetic marker for future selective breeding of triangle pearl mussels.     

Origin and distribution of facial nerve anatomy in van cats

This study aims to reveal the morphological properties of facial nerve and the middle ear in Van cats. Study material was composed of 6 female Van cats. Dissections were performed under a Zoom Stereo Microscope. There was no plexus buccalis in Van cats. The chorda tympani was observed to pass through an opening in the tympanic cavity, emerge through a small opening just behind the retroarticular process, and join the lingual nerve. A rounded anatomical formation with a size of 2.75 ± 0.3 mm was found to be located within the mastoid process of the temporal bone between the facial nerve and the auricular branch of the vagus nerve. The stapes nerve was not present. The geniculate ganglion was very prominent and about 1.00 mm high. The deep petrosal nerve was observed to emerge from the plexus tympanicus. The bulla tympanica was 18.96 ± 0.10 mm long, 13.03 ± 0.20 mm wide and 13.16 ± 0.20 mm high. After leaving the mandibular nerve, the n.tensoris tympani coursed caudally around the a.maxillaris, formed an ansa, entered the tympanic cavity through the canalis musculotubarius and reached an end in the m. tensor tympani. Due to the scarcity of studies on the middle ears of Van cats, it is thought that this study will fill a gap in the field of veterinary anatomy.     

Immunohistochemical expression of HER - 2 and Ki - 67 in granulosa cell tumor in bitches

Granulosa cell tumour, an ovarian neoplasm of stromal origin, is an important tumour related to oestrogenic dominance syndrome and cystic endometrial hyperplasia–pyometra complex. In order to analyse ovarian tumour´s malignant potential, immunohistochemical markers can be used, such as anti-HER2 and anti-Ki-67. The aim of this study was to evaluate the expression of immunohistochemical markers HER-2 and Ki-67 in granulosa cell tumour from bitches´ ovaries. In HER-2 immunomarker analysis using the HercepTest^® method, most tumours were classified as 2+ (moderate labelling). Concerning Ki-67 immunomarker, only one case was described as having a high proliferative index. An association was found between immunostained cell percentage by anti-HER-2 antibodies and high pleomorphism, represented by the pattern of follicular/trabecular tumour arrangement. There was no correlation between anti-Ki-67 and anti-HER-2 antibody immunostaining intensities, probably due to only one case with a high Ki-67 index. With an effective protocol for HER-2 and Ki-67 immunohistochemical identification in granulosa cell tumours in bitches, it was possible to characterize this neoplasm proliferation profile.     

部分商品化非洲猪瘟病毒荧光PCR检测试剂盒的比对

为获得商品化非洲猪瘟病毒(ASFV)荧光PCR检测试剂盒的敏感性、特异性、一致性和最低检测限等试验数据,以世界动物卫生组织(OIE)推荐引物探针为金标准,以62份已灭活的ASFV阳性田间样品、32份阴性田间样品和P72基因核酸标准物质为检测对象,对农业农村部公布许可的其中17个厂家生产的商品化ASFV荧光PCR检测试剂盒开展了试验特性方面的比对研究。结果显示,17个厂家生产试剂盒的敏感性最低为77.4%,最高为96.8%,特异性最低为87.5%,最高为96.8%,均未达到100%,且有一定差别;12个厂家的试剂盒与OIE推荐引物探针检测结果一致性较好(Kappa值≥0.75),其余5个厂家的试剂盒与其一致性一般(0.4Kappa值0.75);17个厂家的试剂盒均可检测到的最低稀释度为5.9×10~3×2~(-3)拷贝/μL,但不同厂家试剂盒的最低检测限有所差别,其中5个试剂盒可检测到的最低稀释度为5.9×10~3×2~(-3)拷贝/μL,2个试剂盒为5.9×10~3×2~(-4)拷贝/μL,6个试剂盒为5.9×10~3×2~(-5)拷贝/μL,3个试剂盒为5.9×10~3×2~(-6)拷贝/μL,1个试剂盒至少为5.9×10~3×2~(-7),且大部分国产试剂盒的最低检测限比进口试剂盒低。本研究为ASFV荧光PCR检测试剂盒的筛选和比对提供了参考数据。     

Production,chemical composition,and fatty acid profile of milk from buffaloes fed with cupuaçu (Theobroma grandiflorum) cake and murumuru (Astrocaryum murumuru) cake in the Eastern Amazon

The objective was to evaluate the effect of concentrate supplementation using by-products of the Amazonian industry on milk production, milk composition, and milk fatty acid profile of dairy buffaloes. Twelve lactating buffaloes (544.5 ± 35.6 kg, 6.4 ± 2.2 years old, 59 ± 6 days in milk) were allotted in a pasture of Mombaça grass and managed under rotational grazing (4 days occupancy/28 days rest). A 3 × 3 Latin square was adopted, and each animal alternately received three supplementary treatments based on corn bran + soybean meal or cupuaçu cake or murumuru cake for 21 days per treatment. Murumuru cake increased the levels of lauric acid and myristic acid in the milk (p < 0.05). Murumuru cake reduced the unsaturated fatty acid contents in the milk compared with animals fed control diet or cupuaçu cake (24.27% vs. 25.24% vs. 25.08%). The n-6/n-3 ratio was 2.6, 1.97, and 2.0 in the control, cupuaçu, and murumuru groups, respectively. Based on this parameter, cakes made from cupuaçu as well as murumuru could be considered to be adequate for inclusion in dairy water buffalo feed. However, the murumuru cake addition requires some caution because its use induces the secretion of higher levels of lauric and myristic fatty acids that are related to human cardiovascular disease.     

猪场生物安全体系中十个通道的设计应用

2018年8月开始,非洲猪瘟在我国快速多点发生,该病是高度接触性传染性疾病,目前无有效的疫苗和药物控制,各大公司和养殖户开始了猪场生物安全体系的升级改造。两年后我国大部分猪场的成功复产,全国生猪出栏量的大幅提升,证明了生物安全措施巨大效力;同时在严格的生物安全措施下创新了精准剔除、快速复产等操作,进一步证明了生物安全措施的有效作用。文章主要介绍猪场生物安全体系中入口(大门)和出口(后门)最为关键防线处的通道设计,方便所有的生物安全操作能够高效落实,切断病原轻易进出猪场的途径。