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31.
Effect of the route of administration on the mucosal and systemic immune responses to Lawsonia intracellularis vaccine in pigs 下载免费PDF全文
In an on‐farm study, 40 weaned piglets aged 3 weeks were vaccinated with Lawsonia intracellularis vaccine orally, IM or IP while a fourth group remained unvaccinated. All vaccinated animals showed increased serum levels of L. intracellularis‐specific IgG antibodies, but significantly elevated concentrations of specific IgG, IgA and cytokines were generated in ileal mucosal secretions from the orally and IP vaccinated pigs when examined at 17 days after vaccination. 相似文献
32.
Luiz Sergio Almeida Camargo Fernanda Queiros Costa Michele Munk Sabine Wohlres‐Viana Raquel Varela Serapio Bruno Campos Carvalho Paulo Henrique Campos Alex Cabral Vieira Luiz Altamiro Garcia Nogueira Joao Henrique Moreira Viana 《Reproduction in domestic animals》2019,54(10):1357-1365
This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus–oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleavage at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes. 相似文献
33.
Nogueira I Lobo-da-Cunha A Afonso A Rivera S Azevedo J Monteiro R Cervantes R Gago-Martinez A Vasconcelos V 《Marine drugs》2010,8(10):2721-2732
Neurotoxicity induced in fish by domoic acid (DA) was assessed with respect to occurrence of neurotoxic signs, lethality, and histopathology by light microscopy. Sparus aurata were exposed to a single dose of DA by intraperitoneal (i.p.) injection of 0, 0.45, 0.9, and 9.0 mg DA kg(-1) bw. Mortality (66.67 ± 16.67%) was only observed in dose of 9.0 mg kg(-1) bw. Signs of neurological toxicity were detected for the doses of 0.9 and 9.0 mg DA kg(-1) bw. Furthermore, the mean concentrations (±SD) of DA detected by HPLC-UV in extracts of brain after exposure to 9.0 mg DA kg(-1) bw were 0.61 ± 0.01, 0.96 ± 0.00, and 0.36 ± 0.01 mg DA kg(-1) tissue at 1, 2, and 4 hours. The lack of major permanent brain damage in S. aurata, and reversibility of neurotoxic signs, suggest that lower susceptibility to DA or neuronal recovery occurs in affected individuals. 相似文献
34.
TA Corrêa MP Monteiro TM Mendes DM Oliveira MM Rogero CI Benites CG Vinagre BM Mioto D Tarasoutchi VL Tuda LA César EA Torres 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(3):277-282
We compared the effects of medium light roast (MLR) and medium roast (MR) paper-filtered coffee on antioxidant capacity and lipid peroxidation in healthy volunteers. In a randomized crossover study, 20 volunteers consumed 482?±?61?ml/day of MLR or MR for four weeks. Plasma total antioxidant status (TAS), oxygen radical absorbance capacity (ORAC), oxidized LDL and 8-epi-prostaglandin F2α, erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activity were measured at baseline and after the interventions. MLR had higher chlorogenic acids-(CGA; 334?mg/150?mL) and less caffeine (231?mg/150?ml) than MR had (210 and 244?mg/150?ml, respectively). MLR also had fewer Maillard reaction products (MRP) than MR had. Compared with baseline, subjects had an increase of 21 and 26?% in TAS, 13 and 13?% in CAT, 52 and 75?% in SOD, and 62 and 49?% in GPx after MLR and MR consumption (P?0.001), respectively. ORAC increased after MLR (P?=?0.004). No significant alteration in lipid peroxidation biomarkers was observed. Both coffees had antioxidant effects. Although MLR contained more CGA, there were similar antioxidant effects between the treatments. MRP may have contributed as an antioxidant. These effects may be important in protecting biological systems and reducing the risk of diseases related to oxidative stress. 相似文献
35.
Custódio L Escapa AL Fernandes E Fajardo A Aligué R Alberício F Neng N Nogueira JM Romano A 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(1):78-84
This work aimed to evaluate the phytochemical content and to determine the antioxidant and cytotoxic activities of methanol
extracts of the carob tree (Ceratonia siliqua L.) germ flour. The extracts were rich in phenolic compounds, had considerable antioxidant activity, and reduced the viability
of cervical (HeLa) cancer cells. The chemical content and the biological activities of the extracts were significantly affected
by gender and cultivar. Female cultivar Galhosa had the highest levels of phenolic compounds, and the highest antioxidant
activity. Extracts from the hermaphrodite trees and from the female cultivars Galhosa and Costela/Canela exhibited the highest
cytotoxic activity. The most abundant compound was theophylline. The phenolic content was correlated to both antioxidant and
cytotoxic activities. Our findings provide new knowledge about the health implications of consuming food supplemented with
carob germ flour. 相似文献
36.
The effects of temperature and photoperiod on egg hatching success,egg production and population growth of the calanoid copepod,Acartia grani (Calanoida: Acartiidae) 下载免费PDF全文
Natacha Nogueira Bernardo Sumares Carlos Alberto Pestana Andrade António Afonso 《Aquaculture Research》2018,49(1):93-103
Calanoid copepods, including species of the genus Acartia, are commonly used as larval diets for marine finfish. This study aimed to determine the separate effects of water temperature (18, 22, 24, 28° ± 0.5°C) and photoperiod (24L:0D; 18L:6D; 12L:12D; 8L:18D; 0L:24D) on Acartia grani egg production (EP), hatching rate (EHR) and population growth. Egg production rate was not affected by the two abiotic parameters. A. grani eggs incubated at T24°C and T28°C were the first to achieve 50% hatching rate (23–25 hr), with significant differences at the end of the experiment (48 hr) between T28°C treatment (EHR 88 ± 5%) and T18°C treatment (EHR 65 ± 2%). However, different temperature regimes did not affect final number of individuals in population growth experiment. Still, when eggs were excluded from data, population at lower temperatures (18°C) was mainly composed by the nauplii stage (72%), while at higher temperatures (24°C and 28°C) more than 60% of the population was composed by copepodites and adults. A. grani subjected to long‐day photoperiods had significantly lower EHR (16.7% at 24L:0D; 20.8% at 18L:6D) than at short‐day photoperiods (52.6% at 6L:18D; 50.0% at 0L:24D). In population growth experiment, eggs were the most common life stage after 12‐day culture. Lowest population number was found at constant light conditions (665.0 ± 197.1), suggesting higher metabolic rates and depletion of energy reserves in long‐day conditions. This study expanded knowledge on the biological response of A. grani to separate temperature and photoperiod regimes, and provided ground to improve the culture of this potential life feed species for hatcheries. 相似文献
37.
38.
M. E. M. Almeida J. N. Nogueira 《Plant foods for human nutrition (Dordrecht, Netherlands)》1995,47(3):245-256
Objective of this research was to find alternative methods for the control of polyphenol oxidase (PPO) activity in fruits and vegetables with the purpose of reducing or eliminating the use of SO2 for this purpose. Interactions between the use of ascorbic acid, citric acid, EDTA, sodium metabisulphite and heat treatment (70 °C for 2 min) in the control of PPO activity were studied in avocado (var. Fortuna), banana (var. Nanica), apple (var. Ana, Fuji, Gala & Golden), pear (var. D'Agua), peach (var. Real), potato (var. Bintje), eggplant (var. Super F100), mushroom (Agaricus bisporus) and hearts-of-palm (Euterpe edulis Mart.). The results demonstrated that PPO of avocado and eggplant was most resistant to inhibition by the methods used. The least efficient method tested for the control of PPO was the addition of ascorbic acid and EDTA, while the most efficient methods investigated included the use of ascorbic acid, citric acid, sodium metabisulphite and heat treatment. The results indicated that, with the exception of PPO from avocado, the most adequate alternative method to substitute for the use of SO2 in the control of PPO was a combination of ascorbic acid, citric acid and heat treatment. 相似文献
39.
Nilia de la Paz Mirna Fernndez Orestes Lpez Caridad Garcia Antonio Nogueira Leonid Torres Wilfredo Turio Jyrki Heinmki 《Marine drugs》2021,19(6)
We investigated a spray drying process for preparing water-soluble salts of high molecular weight chitosan (CH) intended for pharmaceutical excipient applications. CH was derived from chitin of marine lobster origin (Panulirus argus). The effects of organic acid (acetic or lactic acid) and the ratio (difference) of inlet/outlet air temperature (140/90 °C or 160/100 °C) on spray drying were studied. The yield of spray-dried CH salt powders ranged from 50% to 99% in laboratory and industrial-scale processes. The spray-dried dry powder of CH salts consisted of spherical agglomerated particles with an average diameter of 36.2 ± 7.0 µm (CH acetate) and 108.6 ± 11.5 µm (CH lactate). After dispersing the spray-dried CH salt powder samples in purified water, the mean particle sizes obtained for the CH acetate salts were 31.4 nm (batch A001), 33.0 nm (A002) and 44.2 nm (A003), and for the CH lactate salts 100.8 nm (batch L001), 103.2 nm (L002) and 121.8 nm (L003). The optimum process conditions for spray drying were found: an inlet air temperature of 160 ± 5 °C, an outlet temperature of 100 ± 5 °C and an atomizer disk rotational speed of 18,200 min−1. The X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) results confirmed the amorphous state of the CH salts. The 1H nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectra of CH acetate and lactate salts verified that the spray drying process does not affect the polymer backbone. In conclusion, both laboratory and industrial-scale spray drying methods for preparing water-soluble acid salts of CH are reproducible, and the physicochemical properties of the corresponding CH acid salts are uniform. 相似文献
40.
Martínez Mauro Dinolfo María I. Nogueira Soledad Stenglein Sebastián A. 《植物病害和植物保护杂志》2021,128(5):1377-1381
Journal of Plant Diseases and Protection - The objective of the present work was to evaluate the pathogenicity of Fusarium tricinctum in barley and to determine the potential effects on grain... 相似文献