In vitro adherence of Staphylococcus pseudintermedius to canine corneocytes is influenced by colonization status of corneocyte donors

The current knowledge of in vitro adherence of Staphylococcus pseudintermedius to canine corneocytes is limited to comparative analyses between strains, staphylococcal species or corneocytes collected from different breeds, body sites and hosts. However, the role played by colonization status of corneocyte donors remains unknown. The aim of this study was to evaluate the adherence properties of commensal S. pseudintermedius strains to corneocytes collected from dogs with different colonization status. For this purpose, corneocytes were collected from five dogs that were classified as persistently colonized (D1 and D2), intermittently colonized (D3 and D4) or non-colonized (D5) on the basis of the results of a previous longitudinal study. Adherence to corneocytes originating from each of the five dogs was assessed by an in vitro adhesion assay using four genetically unrelated strains isolated from the colonized dogs (S1 to S4). Irrespective of their host of origin, all strains adhered significantly better to corneocytes from D1 and D2 than to corneocytes from D3, D4 and D5 (P < 0.0001). The mean count of cells adhering to corneocytes from persistently colonized dogs was on average three times higher than the mean count using corneocytes from the other dogs. A significant difference between strains was only observed for one strain-corneocyte combination (S2-D4), indicating that S. pseudintermedius adherence to corneocytes is driven by host factors and only marginally influenced by strain factors. This finding has important implications for understanding and preventing S. pseudintermedius skin colonization and infection.     

Novel “filter pellet” sample preparation strategy for quantitative LA-ICP-MS analysis of filter-bound sediments: a “green chemistry” alternative to sediment fingerprinting in Tanzania’s Ruvu River basin

Journal of Soils and Sediments - The goal of this study was to develop a “green chemistry” alternative sample preparation method for elemental analysis of sediments using laser ablation...     

Greenhouse gas implications of water reuse in the Upper Pumpanga River Integrated Irrigation System, Philippines

Enhancing water productivity is often recommended as a “soft option” in addressing the problem of increasing water scarcity. However, improving water productivity, particularly through water reuse, incurs additional investment and may result in increased greenhouse gas (GHG) emissions. In this study, we analysed the water productivity and GHG implications of water reuse through pumping groundwater and creek water, and compare this with gravity-fed canal irrigation in the Upper Pampanga River Integrated Irrigation System (UPRIIS) in the Philippines.Water productivity indicators show that water reuse contributes significantly to water productivity. For example, water productivity with respect to gross inflow (WP_gross) with water reuse (0.19 kg grain/m³) is 21% higher than without water reuse (0.15 kg grain/m³). However, there is a tradeoff between increasing water productivity and water reuse as water reuse increases GHG emissions. The estimated GHG emission from water reuse (pumping irrigation) is 1.47 times higher than without water reuse (gravity-fed canal irrigation). Given increasing concerns about climate change and the need to reduce carbon emissions, we recommend that a higher priority be given to water reuse only in areas where water scarcity is a serious issue.     

Morph-physiological responses of cotton interspecific chromosome substitution lines to low temperature and drought stresses

Limited knowledge about genetic and physiological traits associated with drought and low temperature stresses and narrow genetic diversity in Upland cotton (Gossypium hirsutum L.) are serious impediments in its genetic improvement. The objectives of this research were to determine the genetic and physiological traits associated with drought and low temperature effects and to identify chromosomal effects on these traits using chromosome substitution (CS) lines from three alien species of Gossypium, G. barbadense, G. tomentosum, and G. mustelinum, respectively. Two experiments were conducted to study low temperature and drought stress effects during seedling emergence and early growth stages in 21 cotton CS-lines with parent, Texas Marker (TM)-1. In Experiment I, plants were grown at optimum (30/22 °C) and low (22/14 °C) temperature conditions under optimum water and nutrient conditions. In Experiment II, plants were grown at optimum water (soil moisture content of 0.167 m³ m^?3) and in drought (soil moisture content 0.105 m³ m^?3) conditions under optimum temperature conditions. Above- and below-ground growth traits including several root traits of the CS lines were assessed at 25 days after sowing. The findings suggest which substituted chromosome or chromosome segment from the alien species likely harbors one or more genes for higher and lower tolerance to low temperature, respectively. CS-T04 and CSB08sh showed higher and lower tolerance to low temperature, respectively and CS-T04 and CS-B22sh showed higher and lower tolerance, respectively, to drought. CS lines are valuable analytical tool and useful genetic resources for targeted exploitation of beneficial genes for drought and low temperature stresses in Upland cotton.     

Group planting of cherry(Prunus avium L.) fosters growth and tree quality is superior to conventional row planting in Germany

Wild cherry trees produce high-quality timber and provide multiple ecosystem services.However, planting and tending cherry stands in conventional rows are too costly.Therefore, low density group planting was trialled as an alternative to row planting.The mortality, growth, and quality of planted cherry trees were compared between the group and row planting.The influence of neighbourhood competition and light availability on growth and quality was studied.The group and row planting of cherry trees were established at a wind-thrown site in southwestern Germany in the year 2000.In group planting, five cherry seedlings and seven lime seedlings(Tilia cordata Mill.) were planted with a 1×1 m spacing.In total, 60 groups were planted per hectare with a 13×13 m spacing.In contrast, 3300 seedlings(2475 cherries and 825 limes) were planted per hectare in row planting with a 3×1 m spacing.Ten groups and plots(10×10 m) were randomly established in group and row planting stand, respectively.The survival rate, stability(height to diameter ratio), diameter, and height growth were significantly higher in group planting.In the group plantings,40.5% of cherry trees had straight stems and 13.5% had a monopodial crown compared with 15% with straight stems and 2% with a monopodial crown in row planting.The proportion of dominant cherry trees in canopy was 49% in groups compared with 22% in rows.The length of branch free bole was significantly higher in cherries planted in groups than those grown in rows.Intra-and interspecific competition reduced the growth and stability of cherry trees in row planting, but not in group planting.Light availability did not cause any significant effects on growth and quality between group and row planting.This first study on cherry group planting indicates that the survival rate, growth, and tree quality were higher in groups than in rows at this early development stage.The competition by naturally born seedlings was an important reason for the difference in performance between group and row planting.This study will encourage forest practitioners to establish more cherry group planting trials on multiple sites to test the effectiveness of this alternative technique as a tool of regeneration and restoration silviculture.     

Effect of miconazole nitrate on immunological response and its preventive efficacy in Labeo rohita fingerlings against oomycetes Saprolegnia parasitica

This study evaluated the effect of sublethal doses of antifungal drug miconazole nitrate (MCZ) on immunological responses and its role as a prophylactic drug against S. parasitica in Labeo rohita fingerlings. Fish were fed with sublethal doses of MCZ, that is, T1—6.30 mgMCZ kgBW^?1, T2—12.61 mgMCZ kgBW^?1 and T3—25.22 mgMCZ kgBW^?1, and sampling was done at different time intervals for 240 hr. Immunological parameters viz. lysozyme activity, oxygen radical production and plasma antiprotease activity showed significant enhancement (p < 0.05) in fish fed with T2 and T3 doses. Expression of immune‐relevant genes such as TLR‐22 and β2‐M showed significantly higher expression at 6 hr and 24 hr of sampling in both liver and head kidney. However, these genes showed a downregulation after 120 hr of sampling in both the tissues. Preventive efficacy study showed that single dose of MCZ provides protection against oomycetes up to the fourth day of infection. Significantly higher mortality was observed in control diet‐fed fish as compared to fish fed with MCZ medicated diet. Thus, it can be concluded that the MCZ can act as a potent antifungal agent for preventing oomycetes infection as well as to enhance the immune response.     

Cage‐pond integration of African catfish (Clarias gariepinus) and Nile tilapia (Oreochromis niloticus) with carps

Cage‐pond integration system is a new model for enhancing productivity of pond aquaculture system. A field trial was conducted using African catfish (Clarias gariepinus) and Nile tilapia (Oreochromis niloticus) in cages and carps in earthen ponds. There were four treatments replicated five times: (1) carps in ponds without cage, (2) tilapia at 30 fish m^?3 in cage and carps in open pond, (3) catfish at 100 fish m^?3 in cage and carps in open pond, (4) tilapia and catfish at 30 and 100 fish m^?3, respectively, in separate cages and carps in open pond. The carps were stocked at 1 fish m^?2. The cage occupied about 3% of the pond area. The caged tilapia and catfish were fed and the control ponds were fertilized. Results showed that the combined extrapolated net yield was significantly higher (P < 0.05) in the catfish, tilapia and carps integration system (9.4 ± 1.6 t ha^?1 year^?1) than in the carp polyculture (3.3 ± 0.7 t ha^?1 year^?1). The net return from the tilapia and carps (6860 US$ ha^?1 year^?1) and catfish, tilapia and carps integration systems (6668 US$ ha^?1 year^?1) was significantly higher than in the carp polyculture (1709 US$ ha^?1 year^?1) (P < 0.05). This experiment demonstrated that the cage‐pond integration of African catfish and Nile tilapia with carps is the best technology to increase production; whereas integration of tilapia and carp for profitability.