梅花花色色素种类和含量的初步研究

特征颜色反应和紫外 可见光谱表明 :梅花花色色素属于黄酮类化合物 ,不含查尔酮、噢、儿茶素、二氢黄酮、二氢黄酮醇等 ,而可能含黄酮、花色苷及其花色素等 ;红、白梅花的花色色素均含相同的非红色的黄酮类化合物 ;梅花的红色花色是源于花色素或 和其苷 ,且红的程度与其花色苷含量成正相关 ;梅花花色差异与总黄酮含量间似乎无明显规律性 .该文可为梅花花色的机理探索及其色素的分子结构鉴定提供参考     

饲喂大豆异黄酮对母猪繁殖性能及哺乳仔猪生长的影响

66头大约克夏母猪和52头二花脸母猪分别按预产期不同间隔地分成试验组和对照组。在试验组大约克夏和二花脸母猪妊娠85d至产后7d间,于基础日粮中分别添加大豆异黄酮8mg/kg和5mg/kg。结果表明,仔猪初生个体重和窝重,同一猪种内试验组与对照组无显著差异(P>0 05),但试验组有增高的趋势;20日龄大约克夏仔猪个体重和窝重,试验组比对照组分别高1 38kg和14 30kg,差异均达极显著水平(P<0 01);20日龄二花脸仔猪个体重和窝重,试验组与对照组差异均不显著(P>0 05)。大约克夏仔猪断奶时(28日龄)个体重和窝重,试验组分别比对照组增加1 21kg和12 23kg,差异均达显著水平(P<0 05);二花脸仔猪断奶时(45日龄)个体重和窝重,试验组比对照组分别增加0 51kg和6 01kg,差异均达显著水平(P<0 05)。同时,大豆异黄酮对大约克夏和二花脸母猪下一胎次的繁殖性能均无不良影响。     

一次雹暴过程的三维数值模拟

利用中国科学院大气物理研究所建立的完全弹性三维雹云数值模式,模拟了2007年7月25日发生在南京市六合县的一次强雹暴过程,分析了风暴的气流场、雷达回波结构、涡度场和含水量等宏观微物理的分布及其演变。通过将模拟结果与多普勒雷达资料进行对比发现,模拟的雷达回波强度及回波顶高与雷达观测资料基本一致。模拟结果表明,在雹云强盛时期,近地面层是以辐散气流为主,中上层是以辐合上升气流为主,气流在顶端转变方向,形成辐散出流;模拟的高含水量中心,对应着雷达回波图上的强回波中心,出现在上升气流最大区附近,动力场与物质场配合得较好。     

Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China

[Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.[Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.[Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei's gene diversity index(H),Shannon's information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.[Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population.     

杀菌剂烟剂防治侧柏叶枯病试验

本文在介绍烟剂使用方法的同时,着重指出了用杀菌剂烟剂防治侧柏叶枯病,宜在该病菌子囊孢子释放盛期的6月中旬,按每公顷15kg用量,于逆增气流强烈的傍晚放烟,可以获得良好的防治效果。杀菌剂Ⅰ号烟剂防治效果优于Ⅱ号烟剂。在丘陵山区郁闭度较大的侧柏林内应用是一种较好的防治方法。     

激素、钙、水杨酸和铵诱导马尾松、黑松抗松材线虫病的研究

诱抗试验结果表明：（１）ＩＢＡ、乙烯、ＩＢＡ＋乙烯对马尾松的诱抗效果达６０％，而ＩＢＡ＋乙烯对黑松苗的诱抗效果为１２５％。（２）Ｃａ２＋＋ＩＢＡ对黑松苗的诱抗效果为３６６７％。（３）除Ｓａ＋ＩＢＡ对黑松苗的诱抗效果为１６７％外，其余Ｓａ的各处理效果均不明显。（４）铵的单用、混用或与Ｃａ２＋混用对马尾松诱抗效果均可达６６８％～１００％。     

水稻细菌性条斑病种子带菌检测技术研究——Ⅱ.离体检测法

经3年研究建立了一套用离体稻叶检测稻种带细条病菌的方法.应用该方法成功地将44份自然发病的细条病病种接种到离体稻叶上并产生菌脓.用1%水琼脂加90 ppm元毒苯?咪唑,在30℃下可使水稻幼苗的离体叶保持15～20 d,可满足细条病接种发病的时间需要.应用纯菌测定该方法的灵敏度,最低可检测浓度为5×10~3Cells/ml.由于选择了最佳的温度、湿度、光照、苗龄及其它有关环境条件,使1.2～4.0g的病种经研碎浸泡后浓缩针刺接种于离体稻叶上在3 d后出现病斑,5.5～8.5 d后产生菌脓.与其它血清学检测方法相比,离体检测法具有直观、可靠、简便、稳定等优点,特别可避免由于种子携带丧失活性的细条病菌或其它病菌而引起的误诊.     

稻白叶枯病菌外泌蛋白激发子的活性测定及其纯化

采用(NH4)2SO4分步沉淀法,从水稻白叶枯病菌的培养液中分离到具有激发子活性的物质。该蛋白处理水稻,可使水稻体内与抗病性相关的苯丙氨酸解氨酶和过氧化物酶活性显著提高;在田间喷洒水稻叶片,可以显著提高水稻对白叶枯病菌的抗性,病斑长度明显降低。经滚动式等电聚焦电泳和离子交换层析分离,生测结果表明第II吸收峰具有生物活性;再经SDS PAGE电泳和考马斯亮蓝染色,第II吸收峰收集物有明显的一条带,相对分子质量为47 9kD。     

海栖热袍菌电转化技术的研究(英文)

[目的]对海栖热袍菌电转化技术进行研究。[方法]采用不同参数即不同的方型电脉冲参数、指数衰退型电脉冲参数和高电压电击参数对海栖热袍菌感受态细胞进行电转化,得到的转化子进行PCR验证。[结果]试验中发现以150V的低压方形电脉冲,室温厌氧条件下电击25ms、1次,有利于T.maritima MS8的转化,但这种以整合质粒进行的转化效率极低。[结论]为提高T.maritima MS8的基因转化效率奠定了基础。     

Comparison of Nutrient Components in the Muscle of Wild and Artificially Reared Takifugu obscurus

[Objective] The aim of this study was to evaluate the nutritive value of Takifugu obscurus and provide the scientific basis for artificially feed research.[Method] The nutrient components and nutritive qualities in the muscle of wild and artificially reared Takifugu obscurus were comparatively analyzed.[Result] The contents of crude protein,crude fat,crude ash,total amino acids,essential amino acids and half essential amino acid in the muscle of wild Takifugu obscurus were all higher than those of the artificially reared.The content of four delicious amino acids in the muscle of wild and artificially reared Takifugu obscurus was 17.59% and 14.44%(percentage of dry weight),respectively.The essential amino acid index(EAAI)was 52 and 40 separately,and the constitutional rate of the essential amino acids met the FAO/WHO standard.Valine was the first limiting amino acid of Takifugu obscurus.[Conclusion] The muscle of wild and artificially reared Takifugu obscurus were all high quality food full of animal protein,while the protein nutritive value of wild Takifugu obscurus was higher than that of the artificially reared.