抑制USP7活性对鸡抗沙门菌感染的影响

本研究旨在阐明去泛素酶USP7对雏鸡抵抗鼠伤寒沙门菌感染的影响。选用3日龄的白来航鸡40只,随机分为2组,每组20只,隔天腹腔注射USP7抑制剂P5091或对照溶剂,连续注射5次,待最后一次注射结束后第4天,对雏鸡人工口服感染鼠伤寒沙门菌,感染24 h后,采集样品,RT-qPCR检测IL-1β、IL-8、TNFα以及NF-κB的mRNA表达,平板涂布法检测雏鸡肝载菌量,H&E染色观察肝和肺的炎症状况。结果表明,P5091抑制USP7活性后,转录因子NF-κB mRNA水平显著降低,并且下游细胞因子IL-1β、IL-8以及TNF-α的表达均下调,雏鸡载菌量下降,H&E染色显示肝与肺炎症反应加剧。综上表明,USP7可能在机体抗沙门菌感染方面起重要作用。     

Coordination of autophagy and the proteasome in resolving endoplasmic reticulum stress

Macroautophagy is a cellular degradation mechanism that involves the delivery of cytosolic components (macromolecules or organelles) by the autophagosome to the lysosome for degradation. In mammalian cells, macroautophagy and the ubiquitin proteasome system are 2 major mechanisms to eliminate abnormal proteins accumulated in pathological conditions. Here, the coordination of the 2 pathways to alleviate endoplasmic reticulum stress is reviewed. Also discussed is the regulatory role of macroautophagy and proteasome activity in cell survival and death, as well as the recent discoveries leading to novel strategies of simultaneous control of the proteasome and autophagy activity in anticancer treatment.     

抗旱耐热白三叶新品种选育初报

鄂牧一号白三叶是以美国瑞加和路易斯安那两个白三叶品种为原始材料，经单株抗性造反份系比较鉴定，多系混合杂交，品种比较和区域试验选育而成，历时１０年。该品种具有高产，抗旱，耐热和粗蛋白含量高等特性，是南方低山丘陵地区有推广应用前景的优良牧草和草坪植物。     

鸡干扰素-γ基因的克隆及其重组表达载体的构建

鸡干扰素-γ(ChIFN-γ)是由鸡体或培养的鸡体细胞在干扰素诱生剂作用下所产生的一类非特异性的功能调节蛋白,具有明显的抗病毒、抗肿瘤、调节机体免疫功能等多种活性.为了拓展ChIFN-γ的抗病毒图谱,探讨基因工程干扰素在家禽养殖业中的应用前景,研究采用可以同时进行原核和真核表达的pQE trisystem系统进行ChIFN-γ的表达,旨在弥补传统干扰素制备方法的缺陷,为安全、高效制备重组ChIFN-γ奠定基础.     

A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci-infected domestic poultry in Zhejiang Province,China

A cluster of Chlamydia psittaci (C. psittaci) cases was reported in Zhejiang Province, China, 2019. This study evaluates the extent of the outbreak and determines the source of infection. Real-time PCR and sequencing of the ompA gene of C. psittaci were performed to identify the cases, the domesticated poultry and close contacts. The index patient was a 76-year-old woman with chronic vertigo, and Case 2 was a 64-year-old female farmer with herpes zoster. Both women bought psittaci-infected chickens or ducks from the same mobile street vendor and raised them for 10 days and 23 days before fever onset. There were no direct contact between the two women. C. psittaci test was positive for the two patients, one sick chicken, three healthy ducks and the vendor's chicken cage. Phylogenetic analysis showed that all seven C. psittaci positive samples carried identical ompA genotype A of C. psittaci. Of all of the patients' 148 close contacts, none tested positive for C. psittaci, or developed acute respiratory symptoms. Both patients were discharged after a 4-week hospital stay. In conclusion, the source of this cluster was the poultry infected with C. psittaci, which occasionally cause infections in farmers, but inter-human transmission seems unlikely.     

不同的干燥工艺对荷花蜂花粉功能成分的影响

探讨了5种干燥工艺对荷花蜂花粉功能成分的影响。微波干燥的干燥效率最高,60%火力处理540s可使水分从31.41%降到6.42%;干燥方式对荷花蜂花粉蛋白质影响较小;微波干燥后总黄酮提取率最高,为13.20mg/g;真空冷冻干燥后维生素C和过氧化氢酶含量最高,分别为48.89mg/100g和29.86mg/g·min。总的来说,真空冷冻干燥对荷花蜂花粉的功能成分影响最小;常压恒温干燥对功能成分影响较小、工艺简单、对设备要求低,宜于大规模应用于实际生产中。     

日粮添加免疫生长促进剂C96对雏鸡若干血液指标的影响

选择１２０只１日龄ＡＡ商品健康雏鸡,随机分为４组,每组３０只。初步观察了日粮添加免疫生长促进剂Ｃ９６对血液中红细胞总数（ＲＢＣ）、比容（ＰＣＶ）、血红蛋白含量（Ｈｂ）、网织红细胞比例（ＲＣＣ）、白细胞总数（ＷＢＣ）等的影响。结果表明：肉雏鸡在１５ｄ时,日粮添加Ｃ９６为１１ｍｇ／ｋｇ,饲料的Ｃ９６组和疫苗－Ｃ９６组,与对照组和疫苗组比较,ＲＢＣ和Ｈｂ增高,ＷＢＣ显著下降,ＲＣＣ降低,ＰＣＶ无明显规律性变化;３０ｄ和４５ｄ时,添加Ｃ９６组和疫苗－Ｃ９６组的ＲＢＣ、Ｈｂ和ＰＣＶ,与对照组和疫苗组基本相近,ＷＢＣ和ＲＣＣ均比对照组和疫苗组低或明显低。但３０ｄ时疫苗组的ＷＢＣ比对照组高。整个试验期,ＲＢＣ、ＷＢＣ和ＰＣＶ随日龄的增长呈上升趋势,ＲＣＣ呈波动性下降。     

<Emphasis Type="Italic">Echinococcus granulosus</Emphasis>: Immunoprotection accompanyied by humoral and cytokine response against secondary hydatidosis in mice immunized with r<Emphasis Type="Italic">Eg.</Emphasis>myophilin

Objects:  

This study aimed to investigate the immunoprotection of the recombinant Eg.myophilin (rEg.myophilin) and tentatively analyze mechanism of this protection.     

Advances on Detection Methods for Residues of Nitrofurans in Food

Nitrofurans are broad-spectrum antibiotics which have been widely used in food animals to the prevention and treatment of gastrointestinal infections.Due to abuse of drugs by criminals,animals could produce drug-resistant strains,which reduces the role of antibiotics.Meanwhile,excessive drugs residues in food has carcinogenic,teratogenic and mutagenic effects to the human body.In this paper,the damage and the detection methods of the residues of the nitrofurans in food are surveyed.The main detection methods include high performance liquid chromatography,liquid chromatography-mass spectrometry,liquid chromatography tandem mass spectrometry,enzyme-linked immunoassay,colloidal gold immunoassay and fluorescence immunity analysis method.The research status of various detection methods are summarized and the suggestions for further research are put forward.It is expected to provide reference for future research.     

Acaricidal activity of extracts of neem (Azadirachta indica) oil against the larvae of the rabbit mite Sarcoptes scabiei var. cuniculi in vitro

The acaricidal activity of the petroleum ether extract, the chloroform extract and the acetic ether extract of neem (Azadirachta indica) oil against Sarcoptes scabiei var. cuniculi larvae was tested in vitro. A complementary log-log (CLL) model was used to analyze the data of the toxicity tests. The results showed that at all test time points, the petroleum ether extract demonstrated the highest activity against the larvae of S. scabiei var. cuniculi, while the activities of the chloroform extract and the acetic ether extract were similar. The activities of both the petroleum ether extract and the chloroform extract against the larvae showed the relation of time and concentration dependent. The median lethal concentration (LC(50)) of the petroleum ether extract (1.3muL/mL) was about three times that of the chloroform extract (4.1muL/mL) at 24h post-treatment. At the concentrations of 500.0muL/mL, the median lethal time (LT(50)) of the petroleum ether extract and the chloroform extract was 8.4 and 9.6h, respectively.