优质黑猪育种30年工作体会

品种是影响养猪生产中的最重要因素,猪育种是一项复杂且系统的工程,文章从育种素材筛选、育种方案设计、育种方法选择、品种审定以及扩群产业化推广等方面进行详细的阐述,并结合育种经验提出相应猪育种建议。     

黄河河道变迁对黄淮流域城市的影响——以安徽省砀山县为例

历史上黄河以“善淤、善决、善迁”称著于世,北宋之前,以北流为主,南宋时起,改道南泛,清咸丰五年之后,复北流。南泛期间,在黄淮大平原上来回滚动,对砀山河段变迁的考证,印证了河道多次变迁。黄河河道在黄淮地区的变迁对当地造成了严重的影响,主要表现为水灾频繁、摧屋毁稼,沙灾伴之、破坏环境,淹没城市、城址变迁,治理黄河、建设河防。     

基于城市现代有轨电车建设的环境影响分析——以沈阳有轨电车建设项目为例

指出了近年来随着城市的发展,人口和汽车数量不断增多,公共交通体系急需升级,轨道交通成为城市公交系统发展方向之一。以沈阳有轨电车项目为例进行了分析,根据典型路段预测了结果:在有轨电车最高运行时速情况下,距轨道中心线20m处,大部分路段均能满足2类功能区噪声标准要求,个别路段超标原因是本底噪声较大。有轨电车项目对噪声增加的贡献值较小,不会对噪声敏感点产生较大影响。     

Advances on Detection Methods for Residues of Nitrofurans in Food

Nitrofurans are broad-spectrum antibiotics which have been widely used in food animals to the prevention and treatment of gastrointestinal infections.Due to abuse of drugs by criminals,animals could produce drug-resistant strains,which reduces the role of antibiotics.Meanwhile,excessive drugs residues in food has carcinogenic,teratogenic and mutagenic effects to the human body.In this paper,the damage and the detection methods of the residues of the nitrofurans in food are surveyed.The main detection methods include high performance liquid chromatography,liquid chromatography-mass spectrometry,liquid chromatography tandem mass spectrometry,enzyme-linked immunoassay,colloidal gold immunoassay and fluorescence immunity analysis method.The research status of various detection methods are summarized and the suggestions for further research are put forward.It is expected to provide reference for future research.     

转录组的测序方法及应用研究概述

转录组研究是基因的结构和功能研究的基础,转录组测序技术在近年来取得了很大的进展,被应用到多种动植物中。利用其能够获得大量未知序列的优势,已经被广泛应用到未知基因挖掘、分子调控及相关代谢途径中。该研究主要介绍了转录组研究的方法、转录组测序技术的发展及其在动植物中的重要应用,并讨论其存在的问题及发展趋势。     

Effects of Smo gene silencing on cell activity and apoptosis of human cervical carcinoma HeLa cells

AIM: To investigate the effect of Hedgehog (Hh) signaling pathway on the viability and apoptosis of cervical carcinoma cells by shRNA technique to knock down Smoothened (Smo) gene. METHODS: Smo shRNA was used to transfect the cervical carcinoma HeLa cells. The expression of Smo and Gli1 at mRNA and protein levels in the HeLa cells was determined by RT-PCR and Western blot, respectively. The effect of Smo gene silencing on the growth of the cells was measured by MTT assay. The apoptosis and cell cycle were determined by flow cytometry. RESULTS: Compared with control group, the mRNA and protein expression of Smo and Gli1 were evenly reduced obviously after transfected with Smo shRNA for 72 h (P<0.05). The viability of HeLa cells transfected with Smo shRNA was significantly inhibited. The percentages of the cells in G₀/G₁ phase and early apoptosis rate were obviously higher in Smo shRNA transfection group than those in control group. CONCLUSION: Smo gene silencing effectively inhibits the cell growth and induces the apoptosis of human cervical carcinoma cells.     

高剪切复杂流动中的液滴分散浅析

研究高剪切复杂流动中的液滴分散过程及其应用于高压均质机的均质阀设计,考察均质效果。先从物理方程上分析,然后通过建模来验证理论分析的结果。采用UG建模,导入fluent软件进行模拟,改变流道数目、操作压力,以及进口速度,通过流场的流线分析,考察工艺条件的改变对最终均质效果的影响,为新型高压均质机的均质阀设计提供依据。     

乡村振兴战略背景下农村坑塘治理分析研究——以河南睢县实地调查为例

针对农村坑塘"无人治、没钱治、无人管、散乱难治"等方面的难题,以乡村振兴战略为思想指导,结合河长制工作重点,根据农村坑塘高效治理工作对打造生态宜居环境、创建乡风文明、促进产业兴旺、实现农民富裕的重要性。在此基础上,以河南省睢县农村坑塘为例,以全面推行河湖长制促进乡村振兴为目标,把小微水体整治与乡村振兴等工作有机结合,进行水岸同治。按照远村塘、近村塘的分类,提出相应建设模式,并提出农村坑塘建设建议;另外,从"人治"和"法治"两个方面进行"人治巩固基础,法治加深层次"管护模式,为黄淮地区农村坑塘治理提供多种路径,助力乡村振兴。