Activation of repair pathways after neuronal DNA damage induced by bupivacaine

AIM To investigate the activation of related repair pathways after bupivacaine-induced neuronal DNA damage by cDNA gene screening. METHODS The bupivacaine-induced SH-SY5Y neuronal damage and DNA damage model was established. The technique of cDNA microplate array was used to screen the 21 important regulatory factors in the DNA damage repair pathway. Post-analysis of these differentially expressed repair genes for the repair pathway enrichment and distribution was performed. The data were analyzed by GraphPad Prism 6 statistical software to compare differences between groups. RESULTS The viability of SH-SY5Y cells treated with bupivacaine at different concentrations （detected by CCK-8 assay） showed that the IC₅₀ value of bupivacaine was 1.5 mmol/L. The comet assay related index （the comet tail） was increased （P<0.05）, the phosphorylation level of γH2AX protein was increased （P<0.05）, indicating that DNA damage in the SH-SY5Y cells was significantly aggravated after bupivacaine treatment. The results of cDNA microplate assay showed that compared withcontrol group, the differentially expressed genes after bupivacaine treatment were DNA-PKcs, PTEN, NTH1, RAD9, CSB, GADD45, XPD, XPC-HR23B and P53. The analysis showed that these repair genes were mainly concentrated in the following 3 repair mechanisms： base excision repair, nucleotide excision repair, and non-homologous reconstitution. CONCLUSION The repair genes differentially expressed after neuronal DNA damage caused by local anesthetics are mainly concentrated in the pathways of non-homologous end-joining, base excision repair and nucleotide excision repair.     

深松铲减阻性及耕作阻力影响因素研究——基于LS-DYNA

为研究不同触土曲面深松铲的减阻效果及不同工作参数对深松铲耕作阻力的影响,设计了5种典型准线的深松铲结构,并通过ANSYS/LS-DYNA软件对深松铲切削土壤过程进行了仿真,对比分析了不同结构深松铲切削土壤时所受到的阻力,选择出减阻性能最好的深松铲结构。以优选的深松铲作为研究对象,对入土角、工作速度及工作深度等因素进行单因素试验,研究上述因素对耕作阻力的影响。试验结果表明:仿生变曲率深松铲的减阻性能最好,其耕作阻力最小(601 N);入土角为24°时,深松铲耕作阻力最小;耕作阻力随工作速度和工作深度的增加而增大。该文可为深松铲结构的设计以及工作参数的选择提供一定的技术支持。     

斯氏小盲绥螨取食3种花粉和椭圆食粉螨的实验种群生命表

为了研究斯氏小盲绥螨［Typhlodromips swirskii (Athias Henriot)］取食3种不同花粉和椭圆食粉螨［Aleuroglyphus ovatus (Troupeau)］的发育和繁殖,为斯氏小盲绥螨人工大量饲养提供理论依据,在光照L∥D=10 h∥14 h、相对湿度85%,温度(25±1) ℃的条件下, 研究斯氏小盲绥螨取食向日葵花粉、丝瓜花粉、玉米花粉和椭圆食粉螨的生物学特性,并组建其实验种群生命表。结果表明,斯氏小盲绥螨取食向日葵花粉、丝瓜花粉、玉米花粉和椭圆食粉螨均能从卵发育至成螨并繁殖后代,雌螨发育历期分别为10.42、8.32、9.18、14.43 d;平均每雌总产卵量分别为31.20、65.30、40.17、9.62粒;内禀增长率分别为0.134 3、0.150 2、0.144 9、0.089 5;取食丝瓜花粉的净增殖率（R0=45.057 0）、内禀增长率(rm=0.150 2)和周限增长率 （λ=1.162 0）都最大;取食玉米花粉的雌雄性比最大（2.27∶1）;取食椭圆食粉螨的雌雄性比（0.91∶1）、净增殖率（4.578 8）和内禀增长率（0.089 5）都最小。     

石家庄市桥东区地基承载力的可靠度分析

给出了基于随机场理论的在倾斜荷载作用下地基稳定分析的概率模型和可靠分析模型,采用ＪＣ法分析了石家庄桥东区的可靠度指标与土的重度的变异性,水平荷载的变异性、基础宽度和基础埋置深度之间的关系。     

不同催芽处理对酸枣出苗及生长的影响

通过对酸枣种子进行温水浸种、堆放和冬季冷藏等3种催芽处理试验研究,结果表明:酸枣种子经不同方法催芽处理,出苗率和苗高均高于对照,对出苗率影响差异显著;温水浸种、堆放和冬季冷藏等3种催芽处理的出苗率比对照分别提高10、12和25个百分点,苗高比对照分别提高1.1、2和4.4cm;以冬季冷藏催芽效果最好,出苗率达76%,苗高达35.6cm。     

短时高温胁迫对褐飞虱存活及其热激蛋白基因hsp70表达的影响

为探讨短时高温胁迫对褐飞虱成虫存活的影响及热激蛋白基因hsp70在此过程中的作用,通过室内试验观察羽化24 h内的褐飞虱成虫经过不同高温(31~40℃)暴露和不同恢复时间(1~72 h)后的存活及其体内hsp70表达情况,并构建了褐飞虱hsp70实时荧光定量RT-PCR检测体系。结果表明,褐飞虱在31~39℃下暴露2 h对其成虫存活无显著影响,39.5℃下其存活率显著降低,为44.4%,40℃下无成虫存活;在37℃下暴露2 h后,在12 h的恢复时间内褐飞虱的存活率与对照组无显著差异,但随着恢复时间延长存活率迅速下降。缓和高温(31℃和33℃)对褐飞虱成虫hsp70表达具有诱导作用,其表达量分别为对照组的3.8和6.7倍;在35℃和37℃时达到最高峰,为对照组的291.2和232.5倍;当温度达到39℃和39.5℃时hsp70表达量呈下降趋势,但仍为对照组的46.7和26.2倍。褐飞虱37℃处理2 h后,随着恢复时间的延长,hsp70基因表达水平迅速下降,恢复48、72 h时,仅为对照组的0.4和0.7倍。推测该基因的表达在褐飞虱抵抗短时高温胁迫过程中具有重要作用。     

Effects of Armillariella tabescens mycelia on the growth performance and intestinal immune response and microflora of early‐weaned pigs

This study was performed to evaluate effects of Armillariella tabescens (A. tabescens) on the growth performance and intestinal immune response and microflora in early‐weaned pigs when used as feed additive. A. tabescens mycelia were added to basal diets at concentrations of 0%, 0.1%, 0.3% or 0.9% (w/w). A total of 144 commercial cross‐bred piglets were randomly allocated to one of these four diets and fed for 30 days. The growth performance of early‐weaned piglets displayed improvement with diets containing 0.1% and 0.3% dried mycelia powder from A. tabescens. Supplementing with 0.1% or 0.3% A. tabescens mycelia induced a 2.6‐ and three‐fold increase in secretory immunoglobulin A (sIgA) content in the jejunal mucosa, respectively, but had only a marginal effect on sIgA in the ileal mucosa. Expression of interleukin‐2, interferon‐γ, and tumor necrosis factor‐α in the jejunal mucosa were elevated with A. tabescens mycelia administration. Increased amounts of Lactobacillus spp. and Bifidobacterium spp. in the jejunum, and decreased amounts of Escherichia coli in the jejunum and ileum were observed with the administration of A. tabescens‐containing diets. This study demonstrated that A. tabescens had beneficial effects on the growth performance and intestinal microflora of early‐weaned pigs.     

湘西引种月见草不同成熟期种子含油量及组分分析

为选育和栽培适于湘西地区种植的月见草新品种,对辽宁省清原县引进的月见草"油毛1号"种子在湘西种植后的不同成熟期种子含油量及组分进行对比分析。采用索氏提取法测定粗脂肪,采用超临界CO2萃取法测定精油得油率,并用GC-MS分析精油组分含量的变化,结果表明,"油毛1号"在湘西引种后的单枝晚熟种子中含油量和亚麻酸含量均为最高。     

我校高职教育实训教学改革的实践与探索

阐述高职教育实训教学的作用与意义,总结我校高职教育实训教学改革的实践与成效,提出进一步加强我校高职教育实训教学改革的思路。     

8种杀菌剂对番茄灰霉病菌的毒力及田间番茄灰霉病菌对咯菌腈的敏感性

为了解决番茄灰霉病菌的抗性问题,筛选出理想的替代药剂,采用菌丝生长速率法测定了山东寿光和河北徐水两地番茄灰霉病菌对不同类型杀菌剂的敏感性。两地的番茄灰霉病菌对咯菌腈、氟啶胺均高度敏感,对其他不同作用方式的杀菌剂表现出不同的敏感性。用同样方法检测了不同地区的106株番茄灰霉菌对咯菌腈的敏感性,结果显示其EC50值分布在0.003 9～0.044 9μg/mL之间,平均EC50值为(0.014 2±0.008 1)μg/mL。咯菌腈可作为防治番茄灰霉病的候选药剂。