多重PCR检测籽用西葫芦种子带毒及热处理脱毒效果分析

为了明确籽用西葫芦种子携带病毒的情况,以籽用西葫芦种子为材料,采用多重PCR技术检测种子带毒率。同时为了筛选出有效脱除病毒的方法,通过相对定量法分析了不同温度湿热和干热处理对种子携带病毒的钝化效果。结果表明,从7个籽用西葫芦品种种子幼苗中均可检测到CMV、ZYMV及WMV,其检出率分别为67.1%、50.0%和72.3%,多个病毒的复合检出率为66%。不同品种种子幼苗的病毒检出率有明显差异,其中‘粒丰9号’种子幼苗的病毒检出率最低,为60%,而‘京丰9号’,‘瑞丰9号’,‘绿丰9号’及‘金葫360’的病毒检出率均为100%。8种处理均能不同程度降低籽用西葫芦种子幼苗的带毒率,其中干热60、70、75℃,湿热75℃处理同时脱除WMV、CMV和ZYMV的效果最好。     

昆虫对Bt晶体蛋白杀虫作用的影响

本文概述了近年来昆虫肠液蛋白酶、中肠受体蛋白和昆虫抗性方面对苏云金杆菌杀虫晶体蛋白杀虫作用的影响。本期先刊登该文中的第一和第二部分。     

Effects of GOLPH3 gene silencing mediated by lentivirus on proliferation,invasion and metastasis of human gastric cancer cell line SGC-7901

AIM：To investigate the effect of Golgi phosphoprotein 3 (GOLPH3) gene silencing on the proliferation, invasion and metastasis of human gastric cancer SGC-7901 cell in vitro. METHODS：SGC-7901 cells were transfected with lentiviral vector carrying GOLPH3 shRNA to construct a stable GOLPH3-silencing cell line LV-GOLPH3-RNAi. The expression of GOLPH3 at mRNA and protein levelss were detected by real-time PCR and Western blotting, respectively. The cell proliferation was analyzed by MTT assay. Transwell migration and invasion experiments were performed to measure the migration and invasion abilities, respectively. RESULTS：The stable GOLPH3-silencing cell line was successfully established. The expression of GOLPH3 at mRNA and protein levels was reduced significantly (P<0.05), leading to the inhibition of cell proliferation in LV-GOLPH3-RNAi group compared with scrambled group and blank control group, as well as the capacities of migration (56.7±1.5 vs 186.0±3.4 and 183.3±4.2, P<0.05) and invasion (33.5±3.0 vs 85.0±3.9 and 83.1±4.4, P<0.05). CONCLUSION：GOLPH3 silencing suppresses the capacities of proliferation, migration and invasion of human gastric cancer SGC-7901 cells, suggesting that GOLPH3 may be a potential tumor marker and independent prognostic factor.     

绿僵菌R8-4菌株大量培养固相阶段的条件

研究了昆虫病原真菌绿僵菌R8—4菌株液固两相法培养过程中固相培养阶段的条件，包括培养基载体、接种量、接种后基质含水量、培养过程中的温度、光照、通气量等因素对产孢量的影响。结果表明：蛭石和稻壳均可作为固相培养的优良载体，以蛭石为载体的产孢量稍高于稻壳。玉米粉和麦麸组合是该菌的良好营养源，与不同载体配合均可使产孢量达到20×100孢子／g以上；以小米提供营养的不如麦麸加玉米粉，当小米与载体的质量比大于3：1可获得15×100孢子／g的产孢量。液固接种比以8：10最佳；接种后适宜的基质含水量约为55％，产孢量达28．9×10^8孢子／g；在培养过程中，恒温培养时以25℃为宜；最佳温度调节方式是先在25℃下培养16d后转移到21℃下再培养5d，可获得最高产孢量；光照调节对菌株生长和产孢有重要影响，培养前期在暗环境培养14d，再于光照培养7d的产孢量最高；高通气量利于菌株生长和产孢，应保持容器通气口通畅，培养基厚度以〈4cm为宜。     

绿僵菌与椰甲截脉姬小蜂对椰心叶甲的协同控制作用

在室内条件下,研究了金龟子绿僵菌小孢变种Metarhizium anisopliae var. anisopliae侵染和椰甲截脉姬小蜂Asecodes hispinarum寄生两种生防措施之间的相互影响,并对两种措施协同控制椰心叶甲Brontispa longissima的效果进行了评价。结果表明,绿僵菌菌株HK4对椰甲截脉姬小蜂成虫安全;但绿僵菌侵染使姬小蜂对椰心叶甲的寄生率显著降低。在先接种绿僵菌后接入姬小蜂的情况下,椰甲截脉姬小蜂对椰心叶甲的寄生率仅为10.23%,显著低于其单独寄生的寄生率79.55%;绿僵菌对椰心叶甲的侵染率为96.59%,高于绿僵菌单独处理的侵染率84.09%,但不显著。而在先接蜂后接种绿僵菌的处理中,绿僵菌对椰心叶甲的侵染率仅为69.09%,显著低于绿僵菌单独处理。对椰心叶甲的生测结果显示,先接种绿僵菌再接入姬小蜂、先姬小蜂寄生再接种绿僵菌、同时接种绿僵菌和接入姬小蜂3种处理的校正死亡率分别为100%、100%、98.86%,显著高于绿僵菌单独侵染和姬小蜂单独寄生。     

The Genetic Diversity and Phylogenetic Relationships of Five Guangxi Pig Breeds and Three Exotic Pig Breeds by Analysis of mtDNA D-loop Sequences

In order to compare the genetic diversity and phylogenetic relationships between Guangxi pig breeds and three exotic pig breeds,one pair of primers was used to amplify 85 mtDNA D-loop sequences of five Guangxi pig breeds (Bama Xiang pig,Dongshan pig,Guizhong spotted pig,Luchuan pig,Huanjiang Xiang pig),and we downloaded 45 mtDNA D-loop sequences of three exotic pig breeds (Duroc,Yorkshire,Landrace) from GenBank.Then the haplotype,genetic diversity and phylogenetics analysis were carried out.The results showed that 38 haplotypes were identified in 130 sequences,and the mean distances among Guangxi pig breeds were smaller than that among Duroc,Landrace and Guangxi pig breeds.The NJ phylogenetic tree classified all samples into two clusters.One contained all of Guangxi pig breeds and partial Yorkshire,the other was composed wholly of exotic pig breeds,which mean there were two distinct maternal origins.In the cluster of Guangxi pig breeds,Luchuan pig were clustered,and the others were rather dispersive.It indicated that Luchuan pig had simple maternal bloods,and the other breeds had complex maternal bloods,and they had gene flow with each other.The maternal pig resources in Guangxi were not affected by the introduction of exotic pig breeds,and they were still suitable for use as a female parent in their development and utilization.     

车用发动机停缸模式下转速波动仿真

停缸技术可明显改善车用发动机的燃油经济性,但对其动力输出产生影响.通过建立发动机曲柄连杆多体模型,基于示功图和经验公式,分析了模型边界计算方法,仿真了发动机多种停缸模式的转速波动特性.结果表明,停缸工作模式的转速波动主要受发火间隔角的影响,减小工作缸负荷可降低转速波动.     

广西八角中二氧化硫检测及污染情况分析

针对广西南宁高峰市场充斥硫磺八角(Illicium verum Hook.f.)、二氧化硫超标问题,检测广西壮族自治区销售的239批八角中的二氧化硫残留量,以了解目前市面上销售的八角中二氧化硫污染情况.将八角以蒸馏法进行处理,样品中的亚硫酸盐系列物质加酸处理后转化为二氧化硫,以过氧化氢接收,将其氧化为硫酸根离子,采用酸...     

优质高产晚籼新组合博优253高产制种技术

介绍杂交水稻晚籼新组合博优253的亲本测253和博A的特征特性;总结出选择适宜的制种地和理想抽穗扬花期、合理安排父母本播种差期、培育多蘖壮秧、合理密植、培育双强高产群体、预测并调整花期及适时适量喷施“九二○”等高产制种的关键技术。     

裹包贮存对全混合日粮品质的影响

为使中小规模奶牛养殖场和个体养殖户也能用到全混合日粮（Total Mixed Ration,TMR）,研究用拉伸膜将制成的TMR饲料成品进行裹包后存放的效果。TMR饲料初始含水率分别调整为40%、50%和60%,利用裹包技术将新鲜制作的TMR饲料进行打捆裹包,分别于存放后的第0、3、7、15和30 d后进行感官评定、营养物质含量和发酵指标的测定。试验结果表明：各处理TMR饲料裹包存放15 d以内其感官品质表现良好,存放30 d时表层有发霉现象产生;随着贮存时间的延长,TMR饲料中的营养物质含量会由于贮存过程中干物质的损失而有相应的增加;裹包TMR饲料的pH值随存放时间延长表现为显著降低（P＜0.05）,50%初始含水率表现最低的pH值（P＜0.05）,氨态氮含量则表现为显著增加（P＜0.05）,且初始含水率越高,氨态氮生成量越高（P＜0.05）。综合生产、品质和适口性等各方面认为50%含水率为裹包TMR较为适宜的水分含量。因此,裹包技术可以应用于TMR的有效贮存以实现TMR饲养技术在中小规模养殖场和个体养殖户中的应用。