Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates

In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain.     

Establishment and Optimization of INH Epitope Peptide Vaccine for Detection of Antibody

In this study, an indirect ELISA method was established to detect inhibin hormone (INH) epitope peptide vaccine antibody, it would provide oretical reference for the determination of Fine-wool sheep after active immune body INH epitope peptide vaccine antibody. On the basis of the predecessors, using indirect ELISA method to determinate serum INH epitope peptide antibody levels of sheep, and through control different experimental conditions to look for the best experimental conditions. Through explorating the experimental conditions, finally, the testing experiment conditions were determined, which was blocked solution with skimmed milk powder, INH and GnIH synthetic peptides dilution degrees for 20 000 times, the optimum reaction time was 60 min, the best color action time was 15 min. In this experiment, a kind of method to detection antibody in the body after INH active immune sheep was built, it would provide a reference for future research.     

Heavy Metals Residues in Soil,Feed and Beef of the Cattle Industry in Yili area of Xinjiang

In order to understand the situation of heavy metal residues of the beef industry chain in Yili area of Xinjiang.The heavy metals (Pb, As, Hg, Cr and Cd) in soil, feed and beef of the cattle farm were determined using the atomic absorption spectrometry.The results showed that the heavy metal (Pb, As, Hg, Cr and Cd)contents in soil samples were 0.03-0.20, 3.87-8.30, 0.15-0.23, 0.09-0.48 and 0.41-0.86 mg/kg, respectively.The heavy metal (Pb, As, Hg, Cr and Cd) contents in feed samples were 3.01-18.09, 75.35-94.27, 19.65-24.37, 0.06-1.04 and 1.81-8.46 μg/kg, respectively.The heavy metal (Pb, As, Hg, Cr and Cd) contents in beef samples were 0.23-0.54, 1.22-7.12, 0.28-0.53, 0.01-0.27 and 0.02-0.03 μg/kg, respectively.The correlation coefficient of the heavy metal element between soil and feed, feed and beef, soil and beef were 0.96, 0.99 and 0.98, respectively.It was concluded that the heavy metal (Pb, As, Hg, Cr and Cd) contents in soil, feed and beef of the cattle farms in Yili area did not exceed the standard and that in the soil, feed and beef had the high correlations.     

Cloning and Sequence Analysis of Buffalo Keap1 Gene and Investigation of Its Expression Pattern in Different Tissues

In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture.     

吉林省蜜蜂疫病危害现状及发展对策

调查分析了吉林省主要蜜蜂饲养区蜜蜂疫病危害的现状,发现蜜蜂白垩病(56%)、蜂螨(40%)及爬蜂病(20%)为吉林省3大主要蜜蜂病害,同时通过实验室检测病蜂样本发现,蜜蜂微孢子虫的隐性感染情况较为严重,32%的蜂场存在不同程度的感染,应引起重视。蜜蜂敌害中胡蜂危害(42%)较为严重,个别蜂场兼受地胆、蚂蚁及蟾蜍的侵害。蜂病的确诊是目前饲养者面临的第一难题,建议尽快建立蜜蜂疫病防治技术体系,宣传蜜蜂健康养殖技术,加强流行病学及相关基础研究,加大抗病蜂种的培育力度,加快无公害对症蜂药的研究进展,给饲养者养成防患于未然、饲养强群等优良习惯,为蜜蜂产业的健康发展奠定良好的基础。     

中药冲剂抗蛋鸡热应激药效学研究

为探讨中药冲剂抗蛋鸡热应激的药效学特征,按0.6 g/(只·d)剂量经口给予热应激蛋鸡中药冲剂,考察其对蛋鸡生产性能及血液生化指标的影响。结果表明,中药冲剂抗蛋鸡热应激效果良好,平均产蛋率比对照组提高10.08%(P0.01),饲料报酬提高26%(P0.01),并能调节热应激蛋鸡血液p H值、血浆钙含量、血浆皮质醇含量至正常水平。     

Genome-wide Association Study of Teats Number Traits in Kele Pigs

Teats number is one of the most important reproductive traits,and closely related to the economic benefit in pig industry.In order to reveal the underlying genetics of left teats number,right teats number and total teats number traits,a genome-wide association study（GWAS）was performed.Samples of DNA were collected to genotyping for 22 Kele pigs using the Illumina Porcine SNP 60K Chip.The GWAS was performed using a mixed-effects model and linear regression approach.When a genome-wide threshold was determined using the Bonferroni method（P<2.06E-5),4 single nucleotide polymorphism（SNP）markers were potentially associated with left teats number,right teats number and total teat number.However,3 SNPs were significant associated and 18 SNPs were potentially associated in chromosomes level.304 Ensembl genes were retrieved around 1 cM of the associated SNPs.The candidate genes in Wnt and Fgf signaling pathway（BTRC,FGF5,FGF8,BMP3,RASGEF1B and HMGB3）might have effect on target traits.These results provided valuable information about the selective breeding for Kele pigs.     

花椰菜主要性状遗传规律以及性状相关性分析

总结了花椰菜的单球质量、成熟期、株高株展、花球品质等主要性状的遗传规律以及性状之间的相关性,并得出结论:花椰菜主要性状遗传规律以及性状相关性研究对育种研究具有重要指导意义,但必须注重与田间调查相结合,只有将遗传规律与田间调查有机结合才能提高育种效率,提高育种水平。     

松滋市“一站式”农村沼气服务模式建设现状及建议

文章深入的考察了湖北省松滋市“一站式”沼气后续服务模式的具体实现形式和建设现状；通过总结、对比等方式,对该模式做了详细分析和研究,发现“一站式”服务模式在优化人员配置、稳定服务人员等方面取得了良好效果,但仍存在诸如服务意识不到位、服务网点收入保障不足等问题.最后在分析“一站式”模式的优势和不足的基础上,提出了针对性的建议.