兰州市城市污水处理厂污泥中重金属形态分布特征与潜在生态风险评价

通过现场调查采样及室内分析,研究了兰州市七里河安宁污水处理厂和雁儿湾污水处理厂污泥中重金属的形态分布特征,并对其进行了潜在生态风险评价。结果表明,污泥总体呈高有机质、高全N和有效P、高微量营养元素,且Cu、Zn、Cd、Pb、Ni5种重金属的含量均低于污泥农用控制标准,具有很好的农用价值。污泥中Zn、Cd、Pb主要以有机结合态和残渣态存在,而Cu、Ni可交换态所占比例较高,分别为14.95%和14.41%,不稳定态所占比例分别为41.12%和31.04%,表现出较高的生物活性和可利用性。七里河和雁儿湾污泥中重金属的潜在生态风险属于低度风险;Cd、Cu是污泥中重金属潜在生态风险的主要贡献者,在污泥处理处置时应引起高度重视。     

元谋干热河谷植被恢复对土壤酶活性的影响特征

选取5种植被恢复模式,研究了不同模式对土壤酶活性的影响以及土壤肥力与土壤酶活性的关系。结果表明,植被恢复后土壤蔗糖酶、过氧化氢酶和碱性磷酸酶活性相比光板地都有明显提高,顺序依次为酸角〉小桐子〉印楝;同种植被的土壤酶活性表现出根际大于非根际的特性;不同的植被恢复模式和种植方式对酶活性都有较大的影响,等高垄沟模式具有较好的保土保肥能力,创造了良好的微生物环境,增强了土壤酶活性;植被恢复模式下酶活性与土壤肥力之间的关系密切,特别是有机质、全氮与土壤蔗糖酶、过氧化氢酶和碱性磷酸酶的相关性较好。因此,用土壤酶活性来评价植被恢复的效果是可靠的。     

半干旱地区果园土壤Cl-迁移积累与环境因子的关系

为揭示环境因子对果园表层土壤氯离子迁移累积的影响,为预防和改良土壤次生盐渍化提供理论指导和相关依据,研究果树生育期表层土壤不同土层氯离子的变化与环境因子（土温、土壤水分、蒸发量、降雨量、水分亏值、气温、空气相对湿度）的相互关系。结果表明：在土壤0~25cm土层的微域范围内,不同土层对氯离子的迁移与累积具有不同的功能。0~5cm土层为氯离子聚集层,10~15cm土层为氯离子传导层。认为10~15cm土层是半干旱地区环境因子对土壤性质直接作用与影响的临界深度。     

基于圆柱面模型的仿形喷雾植物冠层密度超声量化测试

基于低成本超声波传感器搭建了一套植物冠层超声回波信号检测系统,建立了基于圆柱面叶片分布模型的量化测试台。在正交中心复合设计试验基础上,建立了超声回波信号均值与冠层密度、探测距离的定量关系,即植物冠层密度量化模型。对已建立的植物冠层密度量化模型进行方差分析,结果表明,植物冠层密度量化模型具有显著性,且失拟性不显著。植物冠层密度量化模型决定系数R2和预测模型决定系数R2分别为0. 988 5和0. 911 4,表明试验值和预测值具有良好的一致性。为了验证已建立的植物冠层量化模型的可靠性,于室内测试台进行了4种植物在3种不同测试距离下的冠层密度验证测试,试验结果表明,实测值与模型测量值的相对误差最小为1. 230%,最大为13. 650%,平均相对误差为6. 120%,植物冠层密度量化模型对室内测试台的冠层密度测量有较好适用性。室外选择3棵不同的桂树,每棵树选择9个测试点进行验证测试,试验结果表明,实测密度与模型测量密度的最小相对误差为3. 959%,最大相对误差为20. 600%; 3棵桂树的实测密度与模型测量密度的平均相对误差分别为11. 244%、12. 246%和9. 628%,植物冠层密度量化模型对户外桂树密度测量有较好的适用性。     

盐渍化土壤覆土材料理化性状及其对双孢蘑菇生长的影响

研究山西省当地盐渍化土壤添加草炭土、醋糟、谷糠或高粱壳配制的不同覆土材料(Ⅰ:66.7%草炭土,33.3%盐渍化土壤;Ⅱ:50%草炭土,50%盐渍化土壤;Ⅲ~Ⅴ:每立方米盐渍化土壤分别添加45kg醋糟、谷糠或高粱)的理化性状,并比较不同覆土材料对双孢蘑菇(Agaricus bisporus)生长的影响,以草炭土为对照。从覆土至第一潮菇采收结束,Ⅰ和Ⅱ覆土电导率呈逐渐上升趋势,而Ⅲ~Ⅴ覆土的电导率则一直保持较高水平,为1427~1980μS/cm,第三潮菇采收时,Ⅰ~Ⅴ覆土电导率均显著下降;覆土第6天开始,Ⅰ~Ⅴ覆土的pH明显高于对照,为7.47~8.47;Ⅰ和Ⅱ覆土处理的发菌天数与对照相同,Ⅲ~Ⅴ覆土处理则比对照延长3d;各处理的第一潮菇和第三潮菇的采收时间均为5d,第二潮菇采收时间各处理间略有差异,但总产量均高于对照,其中以Ⅲ覆土处理产量最高,达21.3kg/m~2。试验结果表明,盐渍化土壤添加草炭土、醋糟、谷糠或高粱壳均可作为生产双孢蘑菇的覆土材料。     

Heme oxygenase-1 protects renal function in septic rats via influencing expression of thrombomodulin

AIM: To investigate the protective effect of heme oxygenase-1 (HO-1) on the kidney of septic rats and the influence of HO-1 on the expression of thrombomodulin (TM) in the kidney. METHODS: Sepsis in rats was developed with cecal ligation and puncture (CLP). The septic rats were randomly divided into sham group, CLP group, CLP+HO-1 inducer group and CLP+HO-1 inhibitor group (n=18). The plasma levels of creatinine (Cr), cystatin-C (Cys-C), carboxyhemoglobin (COHb), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and TM, and the changes of prothrombin time (PT) and activated partial thromboplastin time (APTT) in each group were measured. Histopathological examination was performed in the kidney. The expression of TM in the kidney tissue was detected by Western blot. RESULTS: Compared with sham group, significantly elevated plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), shortened PT and APTT (P<0.05), significantly increased microthrombus formation, and lowered TM expression in the kidney (P<0.05) of CLP group were observed. The administration of hemin lowered the plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), prolonged PT and APTT (P<0.05), attenuated microthrombus formation, and up-regulated the expression of TM in the kidney (P<0.05). In contrast, ZnPP had the opposite effects. CONCLUSION: HO-1 increases the expression of TM in the kidney and exerts anticoagulatory and antiinflammatory effects, thereby improving renal function in the septic rats.     

Effects of autophagy inhibitor bafilomycin A1 on macrophages polarization

AIM:To investigate the effect of bafilomycin A1 (Baf A1) on polarization in mouse macrophages RAW264.7. METHODS:The macrophages RAW264.7 were treated with Baf A1, the concentration of M1/M2 polarization related cytokines were determined by ELISA. The markers of M1/M2 polarization in the macrophages were determined by flow cytometry. The formation of autophagicbody was observed by immunofluorescence. Western blot was used to detect the expression of autophagy related protein levels. RESULTS:The concentration of M1 related proinflammatory cytokine tumor necrosis factor-α (TNF-α) was increased significantly after Baf A1 intervention (P<0.01). However, the concentration of M2 related anti-inflammatory cytokines IL-10 and IL-13 showed no significant difference. The double positive rate of CD197 and HLA-DR (M1 marker) positive cells in Baf A1 treated group were significant higher than that in control group (P<0.05), indicated that Baf A1 induced polarization of macrophage to M1. The results of immunofluoresence showed that the autophagosomes formation was notable increased in Baf A1 group (P<0.05), meanwhile Western blot results showed the expression of autophay related protein LC3-Ⅱ but not P62 was marked up-regulated (P<0.05). For autophagy activator rapamycin (Rapa) treated group, autophagosome formation was also significant increased (P<0.05), but the expression of P62 was notable down-regulated (P<0.05). CONCLUSION:Baf A1 induces polarization of mouse macrophage RAW264.7 to M1, which may be related to the inhibitory effect on the formation of autolysosome.     

地下水埋深对夏玉米根冠生长和耗水量的影响

利用排水式蒸渗仪,借助自制的Mariotte瓶装置,通过设置6种地下水埋深控制处理(地下水位埋深分别为0.2、0.4、0.6、0.8、1.01、.2m),探讨了不同地下水埋深对夏玉米的地上部分、根系生物量及耗水量的影响。结果表明,夏玉米株高和冠层叶面积随地下水埋深减小有增加的趋势,但是当地下水埋深减小到一定程度时就会抑制株高、叶面积,地下水埋深0.6m处理株高和叶面积系数(LAI)最大。各土层根系及其总生物量随地下水埋深增大而增大;地上部总生物量与地下部根生物量的比值随地下水埋深的增大呈明显减少的趋势。夏玉米全生育期和各阶段耗水量分别与地下水埋深呈较好的负相关关系,达到极显著水平(P<0.01);地下水补给量占耗水量的比例随地下水埋深的增大而逐步降低。研究结果可为江淮丘陵区夏玉米灌溉制度的制定及农田排水工程的设计提供参考依据。     

高产条件下夏玉米需水量与需水规律研究

依据2009-2010年田间试验资料,利用农田水量平衡方程计算高产条件下夏玉米各生育阶段需水量和全生育期需水量.结果表明,高产条件下,夏玉米全生育期土壤水分维持在田间持水量的80%左右;全生育期需水量为417.30～507.45 mm.各生育阶段需水量分别为:苗期16.80～33.75 mm,占全生育期需水量3.31%...     

不同局部灌溉方式对玉米叶片生理指标的影响

以畦漫灌为对照,对隔行喷灌、隔行沟灌、膜上灌溉和穴灌4种局部灌溉方式下玉米的叶片生理指标变化规律进行了分析。结果表明,局部灌溉对玉米叶片的生理活性产生了一定的影响;膜上灌溉叶片的脯氨酸含量和细胞膜相对透性均不同程度低于畦漫灌,叶绿素、丙二醛含量,细胞间隙CO2浓度、净光合速率、气孔导度、蒸腾速率等指标均有不同程度提高。隔行灌溉、隔行喷灌生理指标接近于畦漫灌,穴灌与畦漫灌相比,生理指标变化与膜上灌溉正好相反。