Cellulolytic bacteria have a bio-activating role in the composting process. A study was carried out to isolate and identify cellulolytic bacteria from various sources. The isolates were cultured in the carboxymethyl cellulose (CMC) agar medium and incubated at 30°C for 3–7 days. Based on morphological characteristics of the isolates, maximum diameter of a clear zone around the colony and maximum cellulolytic activity, eight isolate were selected for further studies regarding composting experiments.
Molecular tests based on PCR amplification and sequencing of 16S rRNA gene of isolates showed the closest phylogenetic similarity with the species of Stenotrophomonas rhizophila DSM14405 (99.8%), Brevibacterium halotolerans DSM8802 (99.6%), Achromobacter marplatensis B2 (99.8%), Bacillus methylotrophicus CBMB205 (100%), Pseudomonas azotoformans IAM 1603 (99.7%), Bacillus sonorensis NBRC 101234 (99.8%), Bacillus subtilis KCTC 13429 (100%) and Ochrobactrum thiophenivorans DSM 7216 (99.3%). The study of the isolates impact on the composting of palm wastes in a randomized complete block design with 11 treatments in 3 replications showed that strain IB (B. methylotrophicus) caused a significant decrease in C:N ratio (58%). The increasing of microbial respiration compared with control after 30 days incubation at 37°C showed that the B. methylotrophicus strain IB with cellulolytic characteristics can be applied to hydrolysis of cellulosic biomass in the composting processes. 相似文献
Sunflower is an important oilseed crop, which shows susceptibility to heat stress. In this study, 63 single cross hybrids were evaluated under heat stress condition for 2 years and compared with the two commercial hybrids. Genotype and genotype × environment (GGE) was used to differentiate single cross hybrids on the basis of multiple traits. GGE biplot showed that several single cross hybrids had higher seed yield potential than standard check. Moreover, seed yield per plant (SYP) was related to pollen viability percentage, showing that achene yield was the product of high gametophytic fertility under heat stress. Hybrids having high seed yield potential under heat stress had lower cell membrane injury. GGE biplot for SYP and its components showed that single cross hybrids were characterized into two major groups. Group I was further characterized into two sub group. Group Ia included hybrids with high 100-SW, while group Ib had the hybrids with high number of seeds per head and head diameter. Group II had the hybrids with high kernel weight and kernel to seed ratio. The hybrids could be recommended according to their potential utilization in the seed industry. 相似文献
To investigate the effect of applying 15N-labeled ammonium sulfate with or without a nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on fertilizer use efficiency and crop productivity of different wheat genotypes, a field trial was conducted at the Nuclear Agricultural Department’s farm of Iran in 2013–2014. The treatments included five wheat genotypes with different 13 C isotope discrimination and three fertilizer treatments, an unfertilized control, 15N-labeled ammonium sulfate, and 15N-labeled ammonium sulfate with DMPP in three replications. Soil samples were taken after 2, 4, and 6 weeks after sowing and also at harvest time. Results from 15N experiment showed that DMPP delayed nitrification of ammonium for 42 days. Genotypes with lower discrimination index had greater uptake of ammonium ions which led to increase crop yield and nitrogen fertilizer use efficiency. The results also suggested that the use of DMPP may not be beneficial in some fast growing wheat genotypes. 相似文献
Animal rearing is the major occupation of most population of South Asian countries. Due to lack of resources and limited approach to modern medicine, most of the livestock raisers prefer to use plant-based traditional medicine also referred to as ethnoveterinary medicine (EVM). Indeed, the use of medicinal plants in South Asia dates back to several centuries with documented evidences. However, there is currently a dearth of documentation and compilation of use of medicinal plants for animal diseases in this part of the world. This review aims to provide an up-to-date compilation of common medicinal plants used for the treatment and/or management of common animal diseases in South Asian countries. Extensive literature search was conducted online and relevant data was retrieved from well-known scientific databases. A total of 276 plants belonging to 95 families have been documented to be in common use for managing 14 different categories of animal diseases. Solanaceae, Lamiaceae, Fabaceae, and Leguminosae were most common plant families in terms of their plant species used for EVM. Gastric diseases were commonly reported and accounted for 72 species of plants used for its treatment followed by the miscellaneous disorders category and skin diseases comprising of 65 and 39 plant species respectively. Herbs accounted for 46% of the total plant species, followed by trees (33%), and shrubs (18%). The EVM were applied through different routes of administration; oral administration accounted for 72% followed by topical application 27%, while burning of plant parts to create smoke around animals to repel insects was less common (1%). It is anticipated that the present review will stimulate further ethnoveterinary research among livestock disease management practices in South Asia. 相似文献
Field populations of beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), from Pakistan were assessed for their resistance to the chlorinated hydrocarbon endosulfan, the organophosphates chlorpyrifos and quinalphos, and the pyrethroids cypermethrin, deltamethrin, bifenthrin and fenpropathrin. Using a leaf-dip bioassay, resistance to endosulfan was high during 1998–2000 but declined to very low, to low levels during 2001–2007, following a reduced use of the insecticide. Organophosphates and pyrethroids were consistently used over the past three decades, and the resistance had been increasing to these insecticide classes. Generally, the resistance to chlorpyrifos and pyrethroids remained low from 1998 to 2002–2003, but resistance increased to moderate to high levels from 2003–2004 to 2006–2007. For deltamethrin, resistance was very high during 2004–2007. Quinalphos resistance remained low during 1998–2006. Correlation analysis of LC50 and LC90 values showed a positive correlation between organophosphates and pyrethroids, but no correlation between endosulfan and organophosphates or pyrethroids tested herein. These results suggest that the conventional chemistries should be replaced with new chemistries for the successful management of S. exigua. 相似文献
Twenty-four 10- to 16-week-old calves in a dairy herd in Birjand, Iran, inadvertently received an excessive dose of salinomycin mixed with barley grain. There was 58% mortality within 10 d. The calves had high serum AST, LDH, and CK activities; histopathologic lesions in myocardium, liver, and kidneys; and clinical signs associated with acute and congestive heart failure. 相似文献
This study was performed to evaluate the infectivity of bradyzoites of two Besnoitia caprae isolates, BC-1 and BC-2, to inbred BALB/c mice. Each group of inbred BALB/c mice was inoculated intraperitoneally with 1 × 103, 1 × 104, 1 × 105, 5 × 105 and 1 × 106 of one of the two isolates of B. caprae bradyzoites. The mice were monitored daily for a period of 40 days for survival. After death of each mice, several passages
from its peritoneal washing and tissues were analyzed using ribosomal DNA-specific PCR assay. Marked differences in pathogenicity
between the isolates were seen. All the inbred BALB/c mice infected with BC-2 survived but all the mice that were administered
with 1 × 05, 5 × 105 and 1 × 106 BC-1 bradyzoites were died within 4–9 days post-infection (DPI). Histopathological examination of the tissues of the dead
mice revealed hyperemia and necrosis with presence of mononuclear and polymorphonuclear cell infiltration in myocardium, spleen
and intestines together with interstitial pneumonia and peritonitis. All inbred BALB/c mice in the 1 × 103 and 1 × 104 groups of BC-1 inoculated mice survived and they were euthanized after 40 DPI. Chronic inflammation with infiltration of
mononuclear cells was evident in myocardium, spleen, alveolar septa of the lungs of most of the examined tissues with hemorrhagic
enteritis in the mice infected with 1x106 bradyzoites. The mice infected with different doses of BC-2 were euthanized after 40 DPI and no lesion was seen in histopathological
sections of their organs. All peritoneal washings and examined tissues were PCR positive in BC-1 group. This experiment is
the first report to show inbred BALB/c mice as a relevant model for B. caprae and demonstrates that this strain of inbred BALB/c mice is a suitable animal model for biological studies and examination
of pathogenesis for this species of Besnoitia. The present findings also provide evidence for significant differences between the two isolates of B. caprae.相似文献