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201.
Improving Drought Tolerance of Rice by Designed QTL Pyramiding 总被引:4,自引:0,他引:4
Drought is the most important factor limiting rice yields in the rainfed areas of Asia. To overcome the problem we developed a new strategy ''''designed QTL pyramiding'''' to more efficiently develop drought tolerant 《分子植物育种》2007,5(2):205-206
Drought is the most important factor limiting rice yields in the rainfed areas of Asia. To overcome the problem, we developed a new strategy 'designed QTL pyramiding' to more efficiently develop drought tolerant (DT) rice cultivars. In the first phase of this strategy, we developed large numbers of introgression lines (ILs) in elite backgrounds using backcross (BC) breeding, each of which carries multiple genomic segments for improved DT from a known donor. Then, we genotyped all ILs with SSR markers to track the genomewide pattern of introgression in the DT ILs of 16 BC populations derived from crosses between 2 recipients (IR64 and Teqing) and four different donors. X^2 tests and linkage disequilibrium analyses of introgression in the DT ILs revealed significant frequency shifts at many loci across the genome and non-random associations at the multilocus level as a result of selection for DT in the ILs, which led us to the discovery of putative genetic networks underlying DT in the ILs. The networks each containing all DT loci detected in ILs from a BC population showed some interesting features. 相似文献
202.
Lipoxygenases are nonheme-iron-containing dioxygenases that catalyze the hydroperoxidation of unsatrated fatty acids containing a cis cis- -pentadiene structure producing hydroperoxy acids with conjugated dienes.…… 《分子植物育种》2007,5(2):188-189
Lipoxygenases are nonheme-iron-containing dioxygenases that catalyze the hydroperoxidation of unsatrated fatty acids containing a cis, cis-1,4-pentadiene structure producing hydroperoxy acids with conjugated dienes. LOX activity has been found in a wide range of plants. Typical substrates for LOX in plants are linoleic acid and linilenic acid fatty acids. The function of various LOXs in plants is unknown, but their participation in all stages of plant growth and development has been suggested (Hildebrand, 1989; Siedow, 1991). Some of the physiological processes in whicn lipoxygenses have been implicated include wounding (Saravitz and Siedow, 1996), pathogen attack (Melan et al., 1993), seed germination (Kato et al., 1992), fruit ripening (Ferrie et al., 1994), plant senescence (Paliyath and Droillard, 1992). The study on the role of lipoxygenase in ripening and senescence fruit focused on tomato and strawberry. Cloning LOX gene of cucumber fruit will make us further understand the molecularaction mode of this enzyme during fxuit ripening and senescence. In this paper we isolated the partial nucleotide sequences of cucumber fiuit lipoxygenase gene and discuss the characterization of it. 相似文献
203.
Mai H. ElNaggar Ghada M. Abdelwahab Omnia Kutkat Mohamed GabAllah Mohamed A. Ali Mohamed E. A. El-Metwally Ahmed M. Sayed Usama Ramadan Abdelmohsen Ashraf T. Khalil 《Marine drugs》2022,20(3)
Several natural products recovered from a marine-derived Aspergillus niger were tested for their inhibitory activity against SARS CoV-2 in vitro. Aurasperone A (3) was found to inhibit SARS CoV-2 efficiently (IC50 = 12.25 µM) with comparable activity with the positive control remdesivir (IC50 = 10.11 µM). Aurasperone A exerted minimal cytotoxicity on Vero E6 cells (CC50 = 32.36 mM, SI = 2641.5) and it was found to be much safer than remdesivir (CC50 = 415.22 µM, SI = 41.07). To putatively highlight its molecular target, aurasperone A was subjected to molecular docking against several key-viral protein targets followed by a series of molecular dynamics-based in silico experiments that suggested Mpro to be its primary viral protein target. More potent anti-SARS CoV-2 Mpro inhibitors can be developed according to our findings presented in the present investigation. 相似文献
204.
Hani A. Alhadrami Gaia Burgio Bathini Thissera Raha Orfali Suzan E. Jiffri Mohammed Yaseen Ahmed M. Sayed Mostafa E. Rateb 《Marine drugs》2022,20(3)
The COVID-19 pandemic and its continuing emerging variants emphasize the need to discover appropriate treatment, where vaccines alone have failed to show complete protection against the new variants of the virus. Therefore, treatment of the infected cases is critical. This paper discusses the bio-guided isolation of three indole diketopiperazine alkaloids, neoechinulin A (1), echinulin (2), and eurocristatine (3), from the Red Sea-derived Aspergillus fumigatus MR2012. Neoechinulin A (1) exhibited a potent inhibitory effect against SARS-CoV-2 Mpro with IC50 value of 0.47 μM, which is comparable to the reference standard GC376. Despite the structural similarity between the three compounds, only 1 showed a promising effect. The mechanism of inhibition is discussed in light of a series of extensive molecular docking, classical and steered molecular dynamics simulation experiments. This paper sheds light on indole diketopiperazine alkaloids as a potential structural motif against SARS-CoV-2 Mpro. Additionally, it highlights the potential of different molecular docking and molecular dynamics simulation approaches in the discrimination between active and inactive structurally related Mpro inhibitors. 相似文献
205.
PCB removal,soil enzyme activities,and microbial community structures during the phytoremediation by alfalfa in field soils 总被引:1,自引:1,他引:1
Chen Tu Ying Teng Yongming Luo Xianghui Sun Shaopo Deng Zhengao Li Wuxing Liu Zhihong Xu 《Journal of Soils and Sediments》2011,11(4):649-656
Purpose
An in situ phytoremediation trial was developed in order to investigate the function of alfalfa during a 2-year bioremediation of an agricultural soil contaminated with polychlorinated biphenyls (PCBs). The study was conducted with the aim to better understand the application potential of PCB phytoremediation at field scale. 相似文献206.
Contaminant transport to groundwater systems through the unsaturated zone follows, in most cases, preferential pathways. The
understanding and assessment of this preferential flow mechanism is important to the prevention and solution of resulting
problems. A column study was carried out where a NaCl-tracer solution (33 to 37 mmohs/cm of conductivity) was passed through
an intact soil core under saturated conditions and three different ponding heights ranging from 5 to 30 mm. Outflow rates
varied from 13 to 103 ml/min during the three tests performed. A subsequent dye tracing and slicing of the soil column was
performed. Electrical conductivity measurements assessed indirectly the chloride content of the water outflow. Breakthrough
curves (BTCs) showed short threshold times (less than 20% pore volume time) and asymmetry with respect to the point defined
by C/Co = 0.5 and pore volume time. The BTCs successfully represent the flow measurements through the soil column, this fact
is supported by the subsequent horizontal slicing (5 cm thick) of the intact core. 相似文献
207.
Three soil classification systems—the World Reference Base for Soil Resources (WRB), Soil Taxonomy, and the recent Russian system—were used for the identification of 17 soil profiles in southwestern Poland; all the systems put emphasis on the soil properties as diagnostic criteria. Different soils developed on glaciofluvial plains, loessic uplands, and in the Sudetes Mountains were classified. The best correlation between the classification decisions in the different systems was obtained for the most widespread soils owing to the similarity of the diagnostic criteria, which were essentially close although not coinciding. The most prominent divergence between the systems in both the names and the taxonomic categories of the soils was found for the polygenetic soils and for the soils developing from the lithologically discontinuous parent materials. It was also found that the diagnostic elements differ in terms of their taxonomic importance among the classification systems. 相似文献
208.
Katriina Kyllönen Hannele Hakola Heidi Hellén Markku Korhonen Matti Verta 《Water, air, and soil pollution》2012,223(3):1171-1182
Total gaseous mercury (TGM) fluxes from the forest floor and a boreal wetland were measured by a flux chamber technique coupled
with an automatic mercury vapour analyser. The fluxes were measured at three sampling sites in southern Finland, 61°14′ N,
25°04′ E in summer 2007, with additionally in situ TGM concentrations in the air at one of the sites and mercury bulk deposition
at another. Most of the flux data were collected during the daytime. At one of the sites, diurnal flux behaviour was studied,
and a clear cycle with an afternoon maximum and a night minimum was observed. The highest emissions (up to 3.5 ng m−2 h−1) were observed at the forest floor site having a moss and grass cover. At the wetland and litter-rich forest floor sites,
the emissions were below 1 ng m−2 h−1 and sometimes negative (down to −1.0 ng m−2 h−1), indicating mercury uptake. The measured average fluxes in August were 0.9 ± 1.1 and 0.2 ± 0.3 ng m−2 h−1 for the forest floor sites and wetland sites, respectively. The flux data were compared with the mercury bulk deposition,
which proved to be of the same magnitude, but opposite in sign. At the mossy forest floor site, the extrapolated TGM emissions
were 130% of the Hg deposition in August 2007. Comparison with other studies showed that the fluxes in background areas are
relatively uniform, regardless of measurement site location and method used. Airborne TGM remained at the background level
during the study, with an average value of 1.3 ± 0.2 ng m−3; it frequently showed a diurnal cycle pattern. 相似文献
209.
S. B. Sosorova A. B. Gyninova M. G. Merkusheva L. L. Ubugunov L. N. Boloneva 《Eurasian Soil Science》2012,45(4):376-385
The content of microelements (Mn, Zn, Cu, Co, Ni, Cr, Pb, and Cd) and Fe is determined in the soils and plants of the Lake
Kotokel’ basin. Their content in the soils is proved not to exceed the regional background and the existing MPC and APC. The
content of Cd is revealed to exceed its clarke value for the world soils, which is related to the natural origin of this element.
The concentrations of Mn, Co, and Pb are close to their clarke values, and those of Zn, Cu, Ni, and Cr are lower than their
clarkes. The studied soils are specified by the maximal amount of the mobile forms of microelements. The profile distribution
of the microelements differs depending on the genetic soil type. For Mn, Zn, and Cu, a significant biogenic accumulation is
pronounced in the organic soil horizons. The content of microelements in the aboveground phytomass exceeds the maximal permissible
levels for Mn, Co, Cr, and Fe. The intensity of the microelements absorption by the plants varies widely, being specified
by the high coefficient of the biological adsorption (except for Fe). Mn, Zn, and Cu are accumulated in the plant phytomass
the most intensely. 相似文献
210.
The regularities of extracting humus acids from soils of different types with solutions of sodium pyrophosphate at the equilibrium
pH values of 5–13 were studied. The increase in the humus acids yield from the soils with the increasing alkalinity of the
solution applied is directly related to the capability of acid functional groups (carboxyl and phenol hydroxyl) for dissociation
at definite pH values. The regularities of the changes in the chemical nature and degree of humification of humus acids extracted
from the soils related to the pH of the solution were revealed. A sodium pyrophosphate solution (pH 10) was suggested for
extracting the humus acids most active in the given soil. 相似文献