The beneficial effect of (-)-epigallocatechin-3-gallate in an experimental model of Alzheimer's disease in rat: a behavioral analysis

BACKGROUND: Progressive cognitive decline is one of the hallmark symptoms of Alzheimer's disease (AD) which can be modeled by beta-amyloid injection into specific regions of brain. Since epigallocatechin-3-gallate (EGCG) is a potent antioxidant agent which its role against oxidative stress and inflammation has been shown in prior studies, we tried to determine whether EGCG administration protects against beta-amyloid-induced memory and coordination impairment in rats. METHODS: Animals (male Wistar rats) were divided into four groups: sham operated, EGCG-pretreated sham operated (sham+EGCG), untreated lesion (lesion), and EGCG-pretreated lesion (lesion+EGCG). Animals in lesion, lesion+EGCG, and sham+EGCG groups received sterile saline or saline plus EGCG (10 mg/kg) intraperitoneally one day pre-surgery and every other day for three weeks. The lesion was induced one day after EGCG pretreatment by injection of 4 microl of sterile saline or water containing 2 nmol/microl beta-amyloid (1-40) into the hippocampal fissure. For behavioral analysis, psychomotor coordination (PMC) index and spontaneous alternation behavior were assessed using Rota-rod Treadmill and Y-maze, respectively at the third week post-lesion. RESULTS: We found that beta-amyloid (1-40) injection into hippocampus can decrease these behavioral indexes in lesion group in comparison with sham group which is similar to behavioral changes in AD. On the other hand, pretreatment with EGCG can improve the PMC index and spatial Y-maze alternation in the lesion+EGCG group in comparison with lesion group. CONCLUSION: We concluded that EGCG can be effective in restoring beta-amyloid-induced behavioral derangements in rats regarding coordination and memory abilities.     

Suppression of telomerase activity by pyrimethamine: implication to cancer

BACKGROUND: Although pyrimethamine (Tindurin) appears to be effective in the prevention and treatment of some infectious diseases, very little information exists on its unpredictable properties. We design this study to evaluate its anti-tumoral effect on a model of cell line. METHODS: The cytotoxic influence of Pyrimethamine on prostate cell line was investigated using an in vitro colometric assay. The potential modulatory effects on metastasis, apoptosis, and immortality characteristics of cells were assessed with gelatin zymography, terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay and telomeric repeat amplification protocol, respectively. RESULTS: Cytotoxicity analysis of pyrimethamine revealed a dose-dependent fashion. An apoptotic influence of pyrimethamine was also confirmed by data obtained from TUNEL assay. Dose-dependent inhibitory effect on matrix metalloproteinases (MMP) was seen in pyrimethamine. A potent inhibitory effect of pyrimethamine was also established by data achieved from TRAPeze telomerase detection kit. CONCLUSIONS: Collectively, as induction of apoptosis together with MMP and telomerase inhibition could be indicative of cancer treatment, pyrimethamine might be considered as a chemopreventative agent in cancer.     

Acetylation of soybean lecithin and identification of components for solubility in supercritical carbon dioxide

There is a growing interest to develop environmentally friendly surfactants for utilization with supercritical carbon dioxide (scCO2), which is a "green" solvent with many industrial applications. The goal of the present work was to separate the commonly used soybean lecithin into a phospholipid-rich fraction, acetylate this fraction, and then test its solubility in scCO2 to gauge its suitability as a surfactant for potential scCO2-based applications. Soybean lecithin was first purified by fractionation using acetone and ethanol and then acetylated with acetic anhydride. The acetylated lecithin was further purified by fractionation with acetone to separate the acetylated fraction from the nonacetylated fraction. High-performance liquid chromatography and electrospray ionization mass spectrometry were utilized to characterize these fractions. The various acetylated phospholipid fractions were then tested for solubility in scCO2 under various pressures and temperatures using both a cloud-point and a Fourier transform infrared apparatus. Acetylation was found to increase the solubility of the phospholipids in scCO2, and N-acetylated phosphatidylethanolamine (NAc-PE) was found to be the most soluble component of the acetylated phospholipids.     

Microsatellite analysis of genetic diversity and population genetic structure of <Emphasis Type="Italic">Aegilops tauschii</Emphasis> Coss. in Northern Iran

Genetic diversity and population genetic structure of Aegilops tauschii in Northern Iran were studied based on nine microsatellite loci. A high level of genetic diversity was observed from the accessions collected from six regions (provinces). These accessions include 79 samples of the two subspecies (tauschii and strangulata), the intermediate form (among morphologically distinguished subspecies) and ten accessions of Triticum aestivum. The nine microsatellites revealed a total of 141 alleles, with an average of 15.7 alleles per locus. A comparison of the parameters showing genetic diversity, including the observed heterozygosity (Ho), gene diversity (He) and Shannon’s information index (I) of Ae. tauschii accessions from different provinces in Northern Iran, indicated that subsp. tauschii possesses the highest genetic diversity, followed by intermediate form. Genetic distance between subsp. strangulata and subsp. tauschii was low, confirming high gene flow between these two subspecies. However, intermediate form was more distinct from both of them. It was also found that the genetic diversity of T. aestivum is obviously lower than that of Ae. tauschii accessions. Moreover, the level of genetic diversity for Gilan, Golestan and Mazanderan provinces was higher than for Ardebil, Ghazvin and Semnan provinces, suggesting that these regions may provide a readily available source of potentially useful variation for wheat improvement.     

Red List assessment of nine <Emphasis Type="Italic">Aegilops</Emphasis> species in Armenia

The aims of this study are to determine the geographical and ecological distribution of nine Aegilops species in Republic of Armenia and to make an assessment of their IUCN Red List status, using the IUCN Red list categories and criteria, in order to develop an in situ conservation strategy for wild relatives of wheat in Armenia. Ecogeographic surveys of nine Aegilops species were undertaken over 2 years in Armenia. They included a herbarium survey followed by extensive ground-truthing field surveys where targeted Aegilops species occur. The study showed that of the nine Aegilops species studied, four are threatened and of these, Ae. mutica and Ae. crassa are critically endangered. The latter species may even be extinct in Armenia. Ae. neglecta and A. biuncialis are endangered. Additional studies are required to assess the threat status of Ae. umbellulata. Ae. columnaris was assessed as near threatened, while the remaining species (Ae. triuncialis, Ae. cylindrica and Ae. tauschii) are of least concern. There has been a dramatic decline in the genetic resources of Aegilops species during recent years in Armenia as a result of adverse human impacts such as expansion of agriculture, urbanization and uncontrolled grazing. Several species, especially Ae. mutica and Ae. crassa, should be prioritized in conservation activities in Armenia. Efforts should be made to conserve genetic diversity of crop wild relative species both in situ and ex situ, bearing in mind that their germplasm carries potentially valuable information (traits) that can improve adaptability and productivity of cultivated wheat varieties.     

Evaluation of four different crops’ sensitivity to sulfosulfuron and tribenuron methyl soil residues

ABSTRACT

On the basis of greenhouse bioassays, the sensitivities of root and shoot biomass of barley (Hordeum vulgare L.), corn (Zea mays L.), oilseed rape (Brassica napus L.) and sugar beet (Beta vulgaris L.) to soil-incorporated sulfosulfuron and tribenuron methyl were assessed. Shoot and root biomass production was measured 30 days after emergence. Plant responses, including roots and shoots dry weight per pot, were described by a three parameter log-logistic regression model as a function of sulfosulfuron or tribenuron methyl doses and the relative sensitivities were calculated at the various ED-levels with their associated 95% confidence intervals. The most precise ED-levels were that at ED₅₀ and sensitivity rank was oilseed rape, sugar beet, corn and barley, whatever the chosen response level and herbicide. We calculated relative sensitivities at ED₁₀, ED₅₀ and ED₉₀ for the species of barley, the most tolerant crop, as reference. Comparison of relative sensitivity of crops to both herbicides showed that the sensitivity of these crops was much larger for tribenuron methyl than for sulfosulfuron. Oilseed rape was the most sensitive species (ED₅₀?=?0.202 and 0.179?µg?kg^?1 soil for root dry weight (RDW) response to sulfosulfuron and tribenuron methyl, respectively) while barley was the most tolerant one (ED₅₀?=?1.008 and 3.68?µg?kg^?1soil for RDW response to sulfosulfuron and tribenuron methyl, respectively). Sugar beet and corn had intermediate sensitivity. Also, we demonstrated how important it is to show the confidence intervals of relative sensitivities. In several instances the relative sensitivities, even numerically large, were not significantly different from 1.00. We demonstrate that classifying biotypes as resistant to a herbicide requires the threshold of resistance/susceptible (R/S) of 4.00 only be accepted if confidence intervals do not cover 1.00.     

Biomass,proximate biochemical composition and fatty acid profiles associated with the growth phase of Chlorella salina Butcher and Isochrysis maritima Billard and Gayral isolated from the coastal waters of Penang,Malaysia

Choosing the right algae with the relevant properties and optimal harvest time for specific culture conditions and products is essential. As such, biomass, biochemical composition and fatty acid (FA) profile at different growth stages of locally isolated strains, suitable for aquaculture, Chlorella salina and Isochrysis maritima, were determined. Biomass and moisture content of both species were affected by the growth phase. A particular accumulation trend of proximate biochemical compounds was observed in these two strains. Protein content decreased whereby, as culture aged, more carbohydrate and lipid accumulated in C. salina and I. maritima, respectively. Variations in FA profile were exhibited in C. salina where PUFA was the highest, followed by SFA and MUFA throughout the growth phases. I. maritima had the highest SFA content, followed by PUFA and MUFA during the exponential phase. The essential PUFAs in C. salina were linoleic acid (LA) and α-linolenic acid (ALA) with a low occurrence of γ-linolenic acid (GLA; 0.49–0.78%) and docosahexaenoic acid (DHA; 0.21–0.45%). I. maritima recorded relatively high ALA (4.73–6.71%), GLA (5.05–7.80%) and DHA (5.15–7.02%) with minor presence of arachidonic acid (ARA; 0.45–0.59%) and eicosapentaenoic acid (EPA; 0.43–0.58%). Both C. salina and I. maritima are suitable for aquaculture feeds, but I. maritima was more superior by having EPA and higher DHA in their cells. Harvesting regime at a specific phase must be taken into account to achieve maximum yields of a target compound; thus, for feeding purpose, harvesting both strains at stationary phase is recommended as better PUFA compositions were obtained.

     

Economic feasibility of Pabda and stinging catfish culture in recirculating aquaculture systems (RAS) in Bangladesh

Recirculating aquaculture system (RAS) is a unique approach that offers total control to culture a wide variety of aquatic species. As capital investment is higher for setting up RAS, it is necessary to conduct an economic feasibility analysis based on real data. A structured questionnaire was used to collect all economic and technical data on two catfish species, viz., Pabda (Ompok pabda) and Shing (Heteropneustes fossilis), cultured in an RAS farm. The ultimate objective was to estimate net cash flow, net present value (NPV), profitability index (PI), payback period, discounted payback period, internal rate of return (IRR), and sensitivity analysis to find out the best profitability indicators. The analysis revealed that the IRR were 4% and 16% for Pabda and Shing production, respectively. However, for Pabda and Shing productions, the payback periods were 15 years and 4 months and 6 years and 2 months, respectively. The NPV value was positive for Shing production and negative for Pabda. On the other hand, the PI was lower than one for Pabda production and higher than one for Shing production. Therefore, the results showed that the Shing production in the RAS facility could be economically feasible; however, Pabda production may not be viable in RAS due to lower production and longer culture period. This study may suggest that by increasing the production capacity of the farm and market price of the product through the proper supply chain and by decreasing the initial investment, cost of fingerlings and feed, profitability can be achieved in RAS in Bangladesh.