Trx-1 over-expression attenuates oxidative stress injury in MPP+ -induced PC12 cells by regulating NF-κB signaling pathway

AIM:To investigate the inhibitory effect of thioredoxin 1 (Trx-1) over-expression on oxidative stress injury in 1-methyl-4-phenylpyridinium (MPP⁺)-induced rat pheochromocytoma PC12 cells by regulating NF-κB signaling pathway.METHODS:The PC12 cells were damaged by treatment with MPP⁺ at 1, 3 and 5 mmol/L, and the optimal concentration of 3 mmol/L was selected. The cell viability was measured by MTT assay. The oxidative stress indexes lactate dehydrogenase (LDH) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in the cell culture supernatant were detected, and the protein expression of Trx-1 was determined by Western blot. Lentiviral infection with Ad-Trx-1-GFP sequence was used to establish a model of MPP⁺-treated PC12 cells with Trx-1 over-expression. The effects of Trx-1 over-expression on the cell viability, oxidative stress responses and NF-κB signaling pathway were determined by MTT assay, commercial kits and Western blot. The effects of phorbol 12-myristate 13-acetate (PMA), an activator of NF-κB signaling pathway, on the viability and oxidative stress of PC12 cells were observed. The NF-κB signaling pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used in MPP⁺-treated PC12 cells with Trx-1 over-expression, and the cell viability and oxidative stress responses were measured. RESULTS:The viability of PC12 cells, SOD activity in the supernatant and protein expression of Trx-1 were decreased, while LDH activity and MDA content in the supernatant were increased significantly by treatment with MPP⁺ at 1, 3 and 5 mmol/L. The effect of MPP⁺ at 3 mmol/L and 5 mmol/L was significantly greater than that at 1 mmol/L (P<0.05), and no significant difference between 3 mmol/L and 5 mmol/L was observed (P>0.05). The inhibitory effect of MPP⁺ on the viability of PC12 cells, and the oxidative stress injury and activation of NF-κB signaling pathway induced by MPP⁺ were significantly attenuated by over-expression of Trx-1. The inhibitory effect of MPP⁺ on the viability of PC12 cells and the oxidative stress injury induced by MPP⁺ were promoted by the activation of NF-κB signaling pathway, while the protective effects of Trx-1 over-expression on the MPP⁺-treated PC12 cells were enhanced by the inhibition of NF-κB signaling pathway. CONCLUSION:Over-expression of Trx-1 protects MPP⁺-treated PC12 cells from oxidative stress injury by regulating NF-κB signaling pathway.     

一般均衡与pareto最优关系的探讨

用构建数学模型的方法探讨了私有制经济系统中一般均衡和pareto最优的关系,并以私有制经济第一、第二福利定理的形式对其进行了证明.     

微量元素对烤烟品质效应的影响

为了提高贵州烤烟的品质,通过大田小区试验,以施用Zn、Mo、B、Cu 4种微量元素肥料为材料,研究微量元素对烤烟品质效应的影响,结果表明:不同微量元素影响烤烟不同的化学成分。微量元素配施可以促进烟碱的合成,Mo、B、Cu与Zn肥配施,消除锌的负面影响,保持烟叶有良好的化学成分,有利于提高烟叶品质。根据贵州省烤烟状况,施用微量元素可采取促控结合的调施技术,保障和改善烟叶产量和品质。     

分户热计量系统中室内温度的分析

根据分户热计量系统的供热特点,提出了分户热计量系统中室内设计计算温度的最佳值和适合我国国情的适用设计值;并给出了在调节过程中流量变化系数与室温变化之间的数学关系式。     

玉米杂种优势预测和固定的研究

本文首先从玉米杂种的生理、生化代谢特点和生物学表现进行研究,杂种优势表现的生理基础为,杂种的光合强度和呼吸强度高于亲本自交系,对生化指标的测定表明,杂交种生物学上比自交系耐热能力强。其次,对玉米各性状的遗传规律进行分析研究,包括产量性状,品质性状,抗病性的遗传,了解各性状的遗传规律,可针对育种目标,选育优良自交系,进行杂交种生产,使杂种Fl表现出强大的杂种优势。再次,阐述玉米杂种优势固定方法,包括传统的固定方法,无性繁殖,无融合生殖,多倍体的应用,染色体易位的应用。最后对QTL在杂种优势预测和固定中的作用加以阐述。     

多疣狭口蛙的两性异形

测量和比较采自昆明东北郊的131只成年多疣狭口蛙K a lou la verrucosa标本(72♂59♀),同时野外观察雌雄蛙的伏卧姿势、运动方式和繁殖行为.成年雌雄蛙的体长、吻长、鼓膜直径、内突长、前臂宽、后肢全长和足长7个形态特征差异显著.对与体长相关的前臂宽、后肢全长和足长进一步回归分析表明,雄蛙前臂宽随体长的增长速率显著大于雌蛙,雌蛙后肢全长和足长随体长的增长速率显著大于雄蛙.雄蛙的前臂宽、后肢全长和足长均大于相同体长雌蛙的前臂宽、后肢全长和足长.繁殖期雄蛙前肢内侧及胸腹部布满分泌物,雌蛙无.雄蛙为2/3蹼,雌蛙约为1/3蹼.雄蛙具单咽外声囊,雌蛙无.雄蛙肛部疣粒大而光滑,雌蛙肛部疣粒细长而尖.     

槟榔土壤养分与叶片营养含量变化调查及相关分析研究

为探讨槟榔叶片营养与土壤养分间关系,在海南槟榔种植园采集槟榔与相应土壤样品,包括槟榔不同生产管理水平、正常结果和黄化槟榔区,分析不同生产管理水平和正常结果与黄化槟榔叶片氮、磷、钾和土壤养分含量,以及槟榔营养含量与土壤养分含量相关系数。结果表明:土壤有机质与土壤碱解氮、全磷、速效磷和速效钾均呈极显著正相关关系,全磷与速效磷、全钾与速效钾含量间呈极显著正相关关系,碱解氮、全磷、速效磷和速效钾之间呈极显著正相关关系,全钾与全磷呈显著正相关关系。有机质、碱解氮、全磷、速效磷、全钾、速效钾与叶片全N、P、K都呈极显著正相关关系。因此,槟榔土壤碱解氮、全磷、速效磷和速效钾间存在彼此互相促进的关系;槟榔土壤有机质、碱解氮、全磷、速效磷、全钾和速效钾含量变化对槟榔叶片N、P、N含量会产生正向影响。     

剑湖大型底栖动物群落结构及多样性

在剑湖选择11个采样点,于2011年1月、4月、6月和10月按季度4次对大型底栖动物进行采样调查,共发现大型底栖动物52种,主要为软体动物、水生昆虫和环节动物种类。大型底栖动物的密度和生物量时空分布差异明显,密度表现为冬季＞春季＞夏季＞秋季;生物量表现为夏季＞春季＞秋季＞冬季。各采样点全年密度为16～23840个/m2,最高密度出现在采样点A左,最低密度则在采样点E右;生物量全年为1.68～2343.47 g/m2,以采样点D最高,采样点I最低。Margalef丰富度指数全年为0.10～2.75,最高值出现在秋季,最低值出现在夏季;Shannon -Wiener 指数、Simpson生物指数和Pielou均匀度指数全年分别为0.09～1.09、0.03～0.65和0.03～1.43,最高值均出现在夏季,最低值均出现在冬季。     

一次强雷暴过程中的VWP产品特征分析

[目的]研究一次强雷暴过程中的VWP产品特征分析。[方法]在前人分析的基础上,对2008年4月8日湖南一次强雷暴发生前、维持发展中、消失前的不同阶段的VWP产品特征进行详细的分析。[结果]在雷暴出现前,低层＂ND＂层的破坏预示着水汽含量层的增厚,中层干冷空气的侵入导致了层结的不稳定;雷暴维持期间,低层强的垂直风切变会导致强雷暴发展,边界层冷空气渗透导致雷暴达到最强盛阶段,地面出现冰雹天气;雷暴消失前（17：59后）,整个风场转为连续＂ND＂层的一个趋势表明强对流云已远离雷达所在地,预示着雷暴结束。[结论]该研究为预报员做雷暴预警提供参考依据。