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排序方式: 共有137条查询结果,搜索用时 15 毫秒
101.
Mariana PacificoAssunta Napolitano Milena MasulloFelipe Hilario Wagner VilegasSonia Piacente Lourdes Campaner dos Santos 《Industrial Crops and Products》2011,33(2):488-496
Syngonanthus nitens is a grass-like species, whose flower stems assume a beautiful golden colour when dried, hence their common name “capim dourado”. This plant represents an important source of income for rural communities, especially in Jalapão region, being the scapes used in the craft industry to make, together with buriti palm strips, traditional handcrafts from their coils. Therefore, considering that scapes and not flowerheads are used, we carried out an analytical study to define the metabolite fingerprint of both S. nitens parts, with the aim, on one hand, to identify the molecules responsible for the characteristic golden colour of the capim dourado herb and, on the other hand, to identify the occurrence of interesting constituents in S. nitens flowerheads to increase the value of this part of the plant, considered a waste matter of golden grass handcrafts. Therefore an HPLC-ESI-MSn method, based on high-performance liquid chromatography coupled to electrospray negative ionization multistage ion trap mass spectrometry, was developed to rapidly identify and guide the isolation of the secondary metabolites occurring in flowers and scapes. On the basis of the on-line HPLC-ESI-MSn screening, 17 compounds, including 6 new molecules, were isolated, and their structures were unambiguously elucidated by NMR spectroscopic data. 相似文献
102.
From a biologically active extract from the fungus Fomitopsis rosea, two new lanostane triterpenes were isolated, 3α-(3′-butylcarboxyacetoxy)oxepanoquercinic acid C 1 and 3α-hydroxy-24-methylene-23-oxolanost-8-en-26-carboxylic acid 2, along with three known triterpenes, 3–5, and the epidioxy sterol derivative 6. The structures of the compounds were elucidated based on their spectral properties. All triterpenes demonstrated antibacterial activity against S. aureus but possessed no antiradical activity against DPPH radicals. 相似文献
103.
Immune defense of wild‐caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation 下载免费PDF全文
Ivana MIRKOV Aleksandra POPOV ALEKSANDROV Vesna SUBOTA Dragan KATARANOVSKI Milena KATARANOVSKI 《Integrative zoology》2018,13(2):180-193
Studies of wild animals' immunity often use comparison with laboratory‐raised individuals. Using such an approach, various data were obtained concerning wild Norway rat's immunity. Lower or higher potential of immune system cells to respond to activation stimuli were shown, because of analysis of disparate parameters and/ or small number of analyzed individuals. Inconsistent differences between laboratory and wild rats were shown too, owing to great response variability in wild rats. We hypothesized that wild rats will express more intense immune activity compared to their laboratory counterparts which live in a less demanding environment. To test this, we analyzed the circulating levels of inflammatory cytokine interleukin‐6 (IL‐6), a mediator which has a central role in host immune defense. In addition, we examined the activity of the central immune organ, the spleen, including cell proliferation and production of pro‐inflammatory cytokines interferon‐γ (IFN‐γ) and interleukin‐17 (IL‐17), which are major effectors of cellular adaptive immune response. In order to obtain reasonable insight into the immunity of wild Norway rats, analysis was conducted on a much larger number of individuals compared to other studies. Higher levels of plasma IL‐6, higher spleen mass, cellularity and basal IFN‐γ production concomitantly with lower basal production of anti‐inflammatory cytokine interleukin‐10 (IL‐10) revealed more intense immune activity in the wild compared to laboratory rats. However, lower responsiveness of their spleen cells' proinflammatory cytokine production to concanavalin A (ConA) stimulation, along with preserved capacity of IL‐10 response, might be perceived as an indication of wild rats' reduced capability to cope with incoming environmental stimuli, but also as a means to limit tissue damage. 相似文献
104.
Paulo Augusto Vianna Barroso Lúcia Vieira Hoffmann Rodolfo Barbosa de Freitas Carlos Eduardo de Araújo Batista Milena Ferreira Alves Uiara Cavalcante Silva Francisco Pereira de Andrade 《Genetic Resources and Crop Evolution》2010,57(3):343-349
Gossypium mustelinum Miers ex Watt is a native from Northeastern Brazil and belongs to the primary gene pool of the cultivated cotton. The unique
places where the species was known to occur were visited to plan ex situ and in situ preservation. Two populations at Caicó became extinct, and only eleven individuals were found. At Jaguarari, although one
population became extinct, a new one was localized with approximately 500 adult plants, where a fence avoided cattle feeding.
The population from Macururé consisted of 28 plants protected from animals by thorns of Bromelia laciniosa Mart. ex Schult. f. Thirteen SSR primer pairs amplified 40 alleles, among which 30% were exclusive of one of the populations.
The genetic differences between populations represented 58.3% of the total genetic variation observed. The high genetic distances
are likely to be caused by geographical isolation as well as by the small number of individuals which contribute to new generations,
and consequential genetic drift. 相似文献
105.
Papetti A Daglia M Aceti C Quaglia M Gregotti C Gazzani G 《Journal of agricultural and food chemistry》2006,54(4):1209-1216
The antiradical properties of water-soluble components of both natural and roasted barley were determined in vitro, by means of DPPH* assay and the linoleic acid-beta-carotene system, and ex vivo, in rat liver hepatocyte microsomes against lipid peroxidation induced by CCl4. The results show the occurrence in natural barley of weak antioxidant components. These are able to react against low reactive peroxyl radicals, but offer little protection against stable DPPH radicals deriving from peroxidation in microsomal lipids. Conversely, roasted barley yielded strong antioxidant components that are able to efficiently scavenge free radicals in any system used. The results show that the barley grain roasting process induces the formation of soluble Maillard reaction products with powerful antiradical activity. From roasted barley solution (barley coffee) was isolated a brown high molecular mass melanoidinic component, resistant to acidic hydrolysis, that is responsible for most of the barley coffee antioxidant activity in the biosystem. 相似文献
106.
The interactions between whey protein isolate (WPI) and high methoxyl pectin (HMP) at pH 3.5 were investigated in situ using ultrasound (US) and diffusing wave spectroscopy (DWS). HMP was added to 10% oil-in-water emulsions containing 1% WPI. At neutral pH, no protein-pectin interactions were observed as both molecules are negatively charged, while at pH 3.5 bridging flocculation occurred via electrostatic interactions. Four different stages were distinguished during the addition of HMP in WPI-stabilized emulsions at pH 3.5. At a concentration below a critical value, no interactions were observed. At concentrations >0.02% HMP, a change in the l factor indicated a change in the ordering of the emulsion droplets, influenced by long-range interactions. At higher concentrations (in the range between 0.04 and 0.06% HMP), attenuation showed significant changes in the surface of the oil droplets, changes which affected the droplet-droplet interactions. At pectin concentrations >0.05%, attenuation of sound and 1/l* decreased, while velocity of sound and particle size increased, as a result of bridging flocculation. These results demonstrated for the first time that methods such as US and DWS combined permit the observation of the early stages of the interactions between two biopolymers at the interface. This is significant in light of increasing efforts in engineering complex interfacial layers. 相似文献
107.
Laurie D. Hall M. H. Gao Amin Elizabeth Dougherty Martin Sanda Jana Votrubova Keith S. Richards Richard J. Chorley Milena Cislerova 《Geoderma》1997,80(3-4):431-448
This paper reports a systematic MRI study at 2 tesla of 23 soils, each separately saturated with a known amount of water. The percentage of that water which could be detected using various MR methods was determined by comparison with a liquid reference sample. A pulse-acquire sequence gave a bulk detection of between 47 and 94% of the known water content of saturated soil. Also for bulk measurements, the inversion-recovery sequence used for determining T1 values detected a range of 0.7–75% of the existing soil water. The CPMG sequence with an echo time (TE) of 1 ms used for determining the bulk T2 values gave lower values, in the range of 0.4–66% overall. A spin-echo MRI sequence with a TE of 2.9 ms gave an even lower bulk detection, ranging from 0.2 to 57%. These low values for the detectable water content of bulk saturated soil water reflect the loss of water magnetization which occurs even during short echo time MR sequence at 2 tesla field strength. The source of the above findings was investigated by measurements of the longitudinal (T1) and transverse (T2) relaxation times and spectral linewidths of the soil-water protons, and by conventional analysis of soil properties. The MR parameters of critical importance to water quantification are T2 and T2*, shorter values of which lead to a progressively greater loss of signal intensity for all MR protocols. Those parameters are affected by the following soil chemical and physical features: soil magnetic susceptibility, and the content of free iron oxides, clay, sand, exchangeable cations (K, Na and Ca), and organic matter. The implication of this work is that the only soil water which can be detected quantitatively at 2 tesla using a conventional spin-echo MRI protocol with echo times of 3 ms or longer is that located in the relatively large soil pores. Using the protocols investigated in this work, water in smaller pores will only be detected accurately for soils which have relatively low paramagnetic-metal impurities and/or have low clay content. Future MR studies of soil water should consider the use of other MRI protocols (e.g. for solid state), and measurement at low magnetic fields. 相似文献
108.
Nicolas S. Bloom Milena Horvat Carl J. Watras 《Water, air, and soil pollution》1995,80(1-4):1257-1268
Twenty-seven laboratories from around the world agreed to participate in an intercomparison exercise for total Hg (Hgt) and methyl Hg (MMHg) in pristine lake water. Unfiltered samples from a remote brown water lake in northern Wisconsin (USA) were collected into acid cleaned Teflon® bottles using ultra-clean sample handling techniques. The samples were acidified in the field with 0.4% by volume of pre-analyzed HCl (12N; <5 pg Hg/mL), and sent to the primary reference laboratory (PRL) by overnight mail. Within one week of receipt, the samples were randomized, and 10% analyzed for Hgt and MMHg at the PRL to verify the homogeneity of the set. Each participating laboratory was then sent 3 randomly selected 1 L bottles, while the PRL retained 30, and the secondary reference laboratory (SRL) retained 12 samples. The participating laboratories were asked to analyze each bottle in triplicate for both Hgt and MMHg, reporting all QA data including blanks, spike recoveries, and detection limits. The PRL analyzed samples in triplicate at both the beginning and the end of the analytical window, to provide a controlled estimate of any changes in concentration or speciation over that time. Of the 23 laboratories that returned results, 18 utilized BrCl oxidation, gold trapping, and cold vapor atomic fluorescence (CVAFS) detection for Hgt. Four laboratories reported similar techniques, varying either in detector (cold vapor atomic absorption), or wet chemistry. Only 16 laboratories reported MMHg results, with 15 using a variation of the aqueous phase ethylation, GC separation, and CVAFS detection technique. The results show good convergence between the participating labs for both Hgt and MMHg. For Hgt 18 of 23 labs reported means within 20% of the consensus value and PRL results (1.27±0.18 ng/L and 1.27±0.14 ng/L respectively). For MMHg, 13 of the 16 labs reported results within 20% of either the consensus value (0.420±0.055 ng/L) or the PRL value (0.446±0.041 ng/L). 相似文献
109.
Kerstin Holmgren 《Water, air, and soil pollution》2001,130(1-4):1751-1756
Semi-quantitative biomass-size distributions (BSD's) along a joint axis of individual size provided an integrated illustration of aquatic communities sampled at different taxonomic and trophic levels. The approach was applied within the Swedish ISELAW-programme (integrated studies of the effects of liming acidified waters) to test the general hypothesis that aquatic communities in limed lakes are not systematically different from communities in comparable non-limed circumneutral lakes. Input data included pelagic phytoplankton and zooplankton, sublittoral/profundal macroinvertebrates, and benthic fish, within twelve Swedish lakes (six limed, two acidic and four circumneutral reference lakes). The four compartments were sampled on different spatial scales, but each designed for between-lakes comparisons. There were no clear-cut differences in overall size distribution between the three categories of lakes. The mean BSD of limed lakes was indeed more similar to the mean BSD of circumneutral lakes than to that of two acidic lakes. Due to high variation within categories, however, acifidication status alone can not be used for reliable prediction of BSD in a certain lake. 相似文献
110.
The antifungal activity on Botrytis cinerea of the diterpenoids 3beta-hydroxy-kaurenoic acid and kaurenoic acid, obtained from the resinous exudates of Pseudognaphalium vira vira, was determined. 3beta-Hydroxy-kaurenoic acid reduced the mycelial growth of B. cinerea in solid and liquid media. Additionally, the damage produced by the fungus on the surface of tomato leaves in the presence of the diterpenoids was evaluated. A higher protective effect was observed in the presence of the hydroxylated diterpene. On the other hand, the effect of the diterpenoids on the production of enzymes that participate in the plant infection by B. cinerea was analyzed. p-Nitrophenylbutyrate esterase production was induced by both diterpenoids, whereas laccase production was only induced by the hydroxylated diterpene. In the study of the mechanism of action of these compounds, it was determined that 3beta-hydroxy-kaurenoic acid would produce permeabilization of the cell membrane of B. cinerea. 相似文献