Cytogenetic analysis of a canine haemangioendothelioma: a case report.

An 8-year-old German Shepherd bitch developed a haemangioendothelioma in the mammary region. The cytogenetic characterization of the tumour cells revealed a chromosome number of 80 plus a double minute. Several aberrations were detected: trisomies 4, 35, and 38, a centric fusion 8/22 and a tandem translocation 9/23.     

Characterisation of complex karyotype changes in a canine thyroid adenoma

A 14-year-old German shepherd dog developed an alveolar adenoma of the thyroid. The cytogenetic evaluation of the tumour cells showed two ranges of chromosome numbers. Centric fusions 2/32, 5/26, 6/11, 8/11, 9/23 and 14/31 were G-band identified in this complex change of karyotype.     

Spontaneous rosette formation of pig and guinea pig thymocytes and peripheral blood lymphocytes: influence of proteases, oxidising and reducing agents, cytochalasin B and carbohydrates

Spontaneous rosette formation (SRF) of pig and guinea pig thymocytes (Th) and thymus derived peripheral blood lymphocytes (PTL) was tested under different experimental conditions. Treatment with different proteases (trypsin, chymotrypsin, protease type VII) showed that SRF of pig Th and PTL is inhibited to a much higher extent than that of guinea pig Th and PTL. Cytochalasin B (CB) inhibited SRF of PTL of both species to a higher degree as compared to Th. Different carbohydrates neither influenced SRF of Th nor of PTL in both species indicating that simple carbohydrates are not the recognized component of SRF receptors. Treatment with oxidising and reducing agents did not influence SRF.