全文获取类型
收费全文 | 154篇 |
免费 | 7篇 |
专业分类
林业 | 25篇 |
农学 | 3篇 |
7篇 | |
综合类 | 5篇 |
农作物 | 2篇 |
水产渔业 | 16篇 |
畜牧兽医 | 90篇 |
植物保护 | 13篇 |
出版年
2023年 | 1篇 |
2022年 | 6篇 |
2021年 | 5篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 10篇 |
2014年 | 5篇 |
2013年 | 9篇 |
2012年 | 7篇 |
2011年 | 16篇 |
2010年 | 5篇 |
2009年 | 5篇 |
2008年 | 7篇 |
2007年 | 8篇 |
2006年 | 22篇 |
2005年 | 7篇 |
2004年 | 9篇 |
2003年 | 12篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
排序方式: 共有161条查询结果,搜索用时 31 毫秒
91.
Analysis of the relationship between luminescence and toxicity of <Emphasis Type="Italic">Vibrio carchariae</Emphasis> pathogenic to shrimp 总被引:1,自引:0,他引:1
Tatsuya?Nakayama Nakao?Nomura Masatoshi?MatsumuraEmail author 《Fisheries Science》2005,71(6):1236-1242
ABSTRACT: Aquaculture deterioration is caused by Vibrio harveyi , which is one of the main shrimp pathogens. Vibrio carchariae having the same phenotypic characteristics as V. harveyi was isolated from a shrimp farm and used in this study to investigate the relationship between luminescence and toxicity. Luminescence was verified by monitoring the expression of the LuxR gene. It was found that the relationship between the expression of LuxR and toxicity was the most significant factor related to luminescence. Results showed that LuxR expression was highest after 20 h of culture, which is comparable to the highest scintillation counter luminescence value (20 h). However, toxin levels in the culture broth were highest between 24 h-36 h of culture. Significant decrease in the toxicity level was observed after 36 h. It was also found that there was a decrease in toxicity level after 12 h of incubation at room temperature of the V. carchariae culture supernatant. The effect of the addition of cell-free culture supernatant on the luminescence of V. carchariae was also determined. Results showed that the addition of cell-free supernatant from 24 h-old culture was most effective in inducing and maintaining luminescence. 相似文献
92.
Kien Trung LE Norikazu ISODA Lam Thanh NGUYEN Duc-Huy CHU Long VAN NGUYEN Minh Quang PHAN Diep Thi NGUYEN Tien Ngoc NGUYEN Tien Ngoc TIEN Tung Thanh LE Takahiro HIONO Keita MATSUNO Masatoshi OKAMATSU Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(6):860
The impact of low pathogenicity avian influenza (LPAI) has been confirmed mainly in farms. Unlike apparent losses caused by the high pathogenicity avian influenza (HPAI), the LPAI impact has been hardly evaluated due to underestimating its spread and damage. In 2019, a questionnaire study was conducted in southern Vietnam to identify the specific risk factors of LPAI virus (LPAIV) circulation and to find associations between husbandry activities and LPAI prevalence. A multilevel regression analysis indicated that keeping Muscovy ducks during farming contributed to LPAIV positivity [Odds ratio=208.2 (95% confidence interval: 13.4–1.1 × 104)]. In cluster analysis, farmers willing to report avian influenza (AI) events and who agreed with the local AI control policy had a slightly lower risk for LPAIV infection although there was no significance in the correlation between farmer characteristics and LPAI occurrence. These findings indicated that keeping Muscovy ducks without appropriate countermeasures might increase the risk of LPAIV infection. Furthermore, specific control measures at the local level are effective for LPAIV circulation, and the improvement of knowledge about biosecurity and attitude contributes to reducing LPAI damage. 相似文献
93.
Nakano M Wu H Taura Y Inoue M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):421-425
The objective of this study was to evaluate nuclear reactivity of Mdm2 and p53 proteins by immunohistochemical means in feline mammary gland tumors; 12 adenomas which included 6 adenomatous lesions obtained from the tissue adjacent to adenocarcinomas, and 22 adenocarcinomas. Seven adenomas and 18 adenocarcinomas showed moderate or marked Mdm2 reactivity. Sixteen adenocarcinomas showed moderate to marked p53 reactivity, but 9 adenomas showed none. Discordant Mdm2 overexpression was found in 5 adenomas and 3 adenocarcinomas, although co-overexpression of Mdm2 and p53 was found in 15 adenocarcinomas. These results suggest that nuclear overexpression of Mdm2 is present in the tumors of early stage without p53 overexpression and related to feline mammary gland tumorigenesis. Nuclear overexpression of p53 is more frequent in adenocarcinomas, but not in adenomas. 相似文献
94.
Pathogenicity of border disease virus FNK2012-1 strain isolated from a pig in
the natural host,sheep
Tomokazu TAMURA Junki MINE Shiho TORII Yuri FUJIMOTO Masatoshi OKAMATSU Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):341-343
A first isolation of border disease virus (BDV) in Japan was from a pig on a farm without
keeping any ruminants. Our previous study showed that this BDV, termed the FNK2012-1
strain, replicated inefficiently in swine-derived cells compared with those of ruminant
origin. Pigs inoculated with this virus showed neither clinical symptoms nor viremia. In
this study, we evaluated the pathogenicity of the FNK2012-1 strain in sheep, its natural
host. The inoculated sheep showed clinical symptoms and transient viremia. Seroconversion
was observed in the inoculated sheep. These results suggest that the FNK2012-1 strain was
introduced from sheep and has not yet adapted to swine. Therefore, surveillance of border
disease in Japan is necessary among both the swine and ruminant populations. 相似文献
95.
Genya Watanabe Hiroyuki Kobayashi Masahiro Shibata Masatoshi Kubota Motoni Kadowaki Shinobu Fujimura 《Animal Science Journal》2015,86(4):435-442
Regulation of taste is important for improving meat quality and glutamate (Glu) is one of the important taste‐active components in meat. Here, the effects of dietary lysine (Lys) content on taste‐active components in meat, especially free Glu, were investigated. Fourteen‐day‐old broiler chicks (Gallus gallus) were fed on diets containing 100% or 150% of the recommended Lys content for 10 days. Concentrations of free amino acids in plasma, muscle and liver were measured. The levels of messenger RNAs (mRNAs) for enzymes related to Glu metabolism were determined in muscle and liver. The concentration of muscle metabolites was also determined. The free Glu content in muscle of chicks fed the Lys150% diet was increased by 44.0% compared with that in chicks fed the Lys100% diet (P < 0.01). The mRNA level of lysine α‐ketoglutarate reductase, which is involved in Lys degradation and Glu production, was significantly increased (P < 0.05) in the Lys150% group. Metabolome analysis showed that the Lys degradation products, muscular saccharopine, pipecolic acid and α‐aminoadipic acid, were increased in the Lys150% group. Our results suggest that free Glu content in muscle is regulated by Lys degradation. These results suggest that a short‐term feeding of high‐Lys diet could improve the taste of meat. 相似文献
96.
Kazuki Komatsu Tsugumi Iwasaki Kosuke Murata Hideaki Yamashiro Valerie Swee Ting Goh Ryo Nakayama Yohei Fujishima Takumi Ono Yasushi Kino Yoshinaka Simizu Atsushi Takahashi Hisashi Shinoda Kentaro Ariyoshi Kosuke Kasai Masatoshi Suzuki Maria Grazia Palmerini Manuel Belli Guido Macchiarelli Toshitaka Oka Manabu Fukumoto Mitsuaki A. Yoshida Akifumi Nakata Tomisato Miura 《Reproduction in domestic animals》2021,56(3):484-497
Since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, we have established an archive system of livestock and wild animals from the surrounding ex-evacuation zone. Wildlife within the alert zone have been exposed to low-dose-rate (LDR) radiation for a long continuous time. In this study, we analysed the morphological characteristics of the testes and in vitro fertilization (IVF) capacity of cryopreserved sperm of racoons from the ex-evacuation zone of the FDNPP accident. The radioactivity of caesium-137 (137Cs) was measured by gamma-ray spectrometry, and the measured radioactivity concentration was 300–6,630 Bq/kg in the Fukushima raccoons. Notably, normal spermatogenesis was observed in the seminiferous tubules of the testes, with the germinal epithelium composed of a spermatogenic cell lineage with no evident ultrastructural alterations; freeze-thawing sperm penetration ability was confirmed using the interspecific zona pellucida-free mouse oocytes IVF assays. This study revealed that the chronic and LDR radiation exposure associated with the FDNPP accident had no adverse effect on the reproductive characteristics and functions of male raccoons. 相似文献
97.
Lee DM Kiener HP Agarwal SK Noss EH Watts GF Chisaka O Takeichi M Brenner MB 《Science (New York, N.Y.)》2007,315(5814):1006-1010
The normal synovium forms a membrane at the edges of joints and provides lubrication and nutrients for the cartilage. In rheumatoid arthritis, the synovium is the site of inflammation, and it participates in an organized tissue response that damages cartilage and bone. We identified cadherin-11 as essential for the development of the synovium. Cadherin-11-deficient mice have a hypoplastic synovial lining, display a disorganized synovial reaction to inflammation, and are resistant to inflammatory arthritis. Cadherin-11 therapeutics prevent and reduce arthritis in mouse models. Thus, synovial cadherin-11 determines the behavior of synovial cells in their proinflammatory and destructive tissue response in inflammatory arthritis. 相似文献
98.
Esmot ara Begum Motoki Bonno Makoto Obata Hatsumi Yamamoto Masatoshi Kawai Yoshihiro Komada 《Journal of circadian rhythms》2006,4(1):11-7
Background
Biological rhythmicity, particularly circadian rhythmicity, is considered to be a key mechanism in the maintenance of physiological function. Very little is known, however, about biological rhythmicity pattern in preterm and term neonates in neonatal intensive care units (NICU). In this study, we investigated whether term and preterm neonates admitted to NICU exhibit biological rhythmicity during the neonatal period. 相似文献99.
Kuhara A Okumura M Kimata T Tanizawa Y Takano R Kimura KD Inada H Matsumoto K Mori I 《Science (New York, N.Y.)》2008,320(5877):803-807
Temperature is an unavoidable environmental cue that affects the metabolism and behavior of any creature on Earth, yet how animals perceive temperature is poorly understood. The nematode Caenorhabditis elegans "memorizes" temperatures, and this stored information modifies its subsequent migration along a temperature gradient. We show that the olfactory neuron designated AWC senses temperature. Calcium imaging revealed that AWC responds to temperature changes and that response thresholds differ depending on the temperature to which the animal was previously exposed. In the mutant with impaired heterotrimeric guanine nucleotide-binding protein (G protein)-mediated signaling, AWC was hyperresponsive to temperature, whereas the AIY interneuron (which is postsynaptic to AWC) was hyporesponsive to temperature. Thus, temperature sensation exhibits a robust influence on a neural circuit controlling a memory-regulated behavior. 相似文献
100.
Utiyama Masahiro Fukuyama Tsutomu Yamada Maruo Yasuko Ichino Toshihiro Izumi Katsuyuki Hara Hiroshi Takano Kenichi Suzuki Haruko Aoki Masatoshi 《Water, air, and soil pollution》2004,151(1-4):53-70
Concentrations of ozone and nitrogen oxides, together with air temperature and solar radiation intensity, were measured at several heights on a tower standing through the canopy of a red pine forest in summer and in autumn. In the summer observation, the diurnal variation patterns of ozone concentration both above and below the canopy were all similar and parallel to the solar radiation intensity. Using the data collected immediately above the canopy, deviation from the Leighton relationship and variations of concentration sums [O3] + [NO] and [NO2] + [NO] were examined, and as a result, it was supposedthat ozone was photochemically formed there in the daytime, probably because hydrocarbons emitted from pine trees broke the photostationary state among ozone and nitrogen oxides. The vertical temperature profile exhibited an inversion at the leaf-layer, which must have hindered vertical mixing of the air and made the trunk space more or less isolated from the upper atmosphere. These observations led to an idea that the similarity of the ozone variation pattern at every height was caused by the photochemical formation that proceeded simultaneously above and below the canopy rather than by vertical transport. Such situations of ozone formation were supported by observation of two maximums in the ozone vertical profile, one immediately above the canopy and another in the trunk space. Another feature of the ozone profile was a deep minimum in the leaf layer, which indicated ozone deposition onto leaf surfaces. This study thus revealed concurrence of ozone formation and deposition, and left two potentially important implications worthy of further investigation: (1) a forest is not always a sink but can be a source of ozone in sunlit conditions, and (2) deposition of ozone to trees can take place not only from outside but also from inside of a forest. In the autumn observation, however, the ozone formation was barely recognizable above the canopy and no longer found in the trunk space; in addition, the ozone concentration minimum in the leaf layer disappeared, suggesting that the deposition or removal was dependent on temperature. 相似文献