Evaluation of ground-level and space-borne sensor as tools in monitoring nitrogen nutrition status in immature and mature oil palm

Monitoring nitrogen (N) in oil palm is crucial for the production sustainability. The objective of this study is to examine the capability of visible (Vis), near infrared (NIR) and a combination of Vis and NIR (Vis + NIR) spectral indices acquired from different sensors for predicting foliar N content of different palm age groups. The N treatments varied from 0 to 2 kg per palm, subjected according to immature, young mature and prime mature classes. The Vis + NIR indices from the ground level-sensor that is green + red + NIR (G + R + NIR) was the best index for predicting N for immature palms (R² = 0.91), while Vis indices blue + red (B + R) and Green Red Index from the space-borne sensor were significantly useful for N assessment of young and prime mature palms (R² = 0.70 and 0.50), respectively. The application of vegetation indices for monitoring N status of oil palm is beneficial to examine extensive plantation areas.     

Molecular and serological prevalence of Babesia bovis and Babesia bigemina in cattle from central region of Syria

A total of 207 bovine blood samples were collected from clinically healthy cattle bred in central region of Syria and examined by Giemsa-stained blood smears, nested PCR, ELISA, and IFAT to determine the molecular and serological prevalence of Babesia bovis and B. bigemina. All samples were negative to Babesia spp. by microscopic examination of blood smears. On the other hand, the overall prevalence of B. bovis and B. bigemina was 9.18% and 15.46% by nPCR, 15.46% and 18.84% by ELISA, and 18.36% and 21.74% by IFAT, respectively. Mixed infections were detected in a total of 5 samples (2.4%) by nPCR, 16 (7.73%) by ELISA and 27 (13.04%) by IFAT. Statistically significant differences in the prevalence of the two infections were observed on the basis of age and location. These data provide valuable information regarding the occurrence and epidemiology of B. bovis and B. bigemina infections in Syrian cattle, which can be employed in developing rational strategies for disease control and management.     

Evapotranspiration, seed yield and water use efficiency of drip irrigated sunflower under full and deficit irrigation conditions

Deficit irrigation occurrence while maintaining acceptable yield represents a useful trait for sunflower production wherever irrigation water is limited. A 2-year experiment (2003–2004) was conducted at Tal Amara Research Station in the Bekaa Valley of Lebanon to investigate sunflower response to deficit irrigation. In the plots, irrigation was held at early flowering (stage F1), at mid flowering (stage F3.2) and at early seed formation (stage M0) until physiological maturity. Deficit-irrigated treatments were referred to as WS1, WS2 and WS3, respectively, and were compared to a well-irrigated control (C). Reference evapotranspiration (ET_rye-grass) and crop evapotranspiration (ET_crop) were measured each in a set of two drainage lysimeters of 2 m × 2 m × 1 m size cultivated with rye grass (Lolium perenne) and sunflower (Helianthus annuus L., cv. Arena). Crop coefficients (K_c) in the different crop growth stages were derived as the ratio (ET_crop/ET_rye-grass).

Lysimeter measured crop evapotranspiration (ET_crop) totaled 765 mm in 2003 and 882 mm in 2004 for total irrigation periods of 139 and 131 days, respectively. Daily ET_crop achieved a peak value of 13.0 mm day⁻¹ at flowering time (stage F3.2; 80–90 days after sowing) when LAI was >6.0 m² m⁻². Then ET_crop declined to 6.0 mm day⁻¹ during seed maturity phase. Average K_c values varied from 0.3 at crop establishment (sowing to four-leaf stage), to 0.9 at late crop development (four-leaf stage to terminal bud), to >1.0 at flowering stage (terminal bud to inflorescence visible), then to values <1.0 at seed maturity phase (head pale to physiological maturity). Measured K_c values were close to those reported by the FAO.

Average across years, seed yield at dry basis on the well-irrigated treatment was 5.36 t ha⁻¹. Deficit irrigation at early (WS1) and mid (WS2) flowering stages reduced seed yield by 25% and 14% (P < 0.05), respectively, in comparison with the control. However, deficit irrigation at early seed formation was found to increase slightly seed yield in WS3 treatment (5.50 t ha⁻¹). We concluded that deficit irrigation at early seed formation (stage M0) increased the fraction of assimilate allocation to the head, compensating thus the lower number of seeds per m² through increased seed weight. In this experiment, while deficit irrigation did not result in any remarkable increase in harvest index (HI), water use efficiency (WUE) was found to vary significantly (P < 0.05) among treatments, where the highest (0.83 kg m⁻³) and the lowest (0.71 kg m⁻³) values were obtained from WS3 and WS1 treatments, respectively. Finally, results indicate that irrigation limitation at early flowering (stage F1) and mid flowering (stage F3.2) should be avoided while it can be acceptable at seed formation (stage M0).     

Deficit irrigation under water stress and salinity conditions: The MOPECO-Salt Model

In both arid and semi-arid areas the use of saline water for irrigation is a common practice, even though it may cause a drop in crop yield and progressive soil salinization. In order to determine the most suitable irrigation strategy for higher yield, profitability, and soil salinity management of certain crops, the MOPECO-Salt Model has been developed. This model was first validated in the Eastern Mancha Agricultural System in Albacete (Spain) through a test carried out on onion crop in April-September 2009, where the simulated yield was 2% lower than the observed one. The model was then tested at Tal Amara Research Station in the Central Bekaa Valley Agricultural System (Lebanon) using data from a 5-year experiment on the effects of deficit irrigation on two cultivars of potato (Spunta: July-October 2001, and June-September 2002; and Agria: March-August 2004, 2005, and 2007). Furthermore, these results were compared with those obtained through AquaCrop, which does not currently assess crop response to salinity. Differences between observed and simulated yields were lower than 3% for MOPECO-Salt and up to 12% for AquaCrop. According to findings from simulations, the irrigation strategies without leaching fraction employed in both areas are remediable since the off-season rainfall is sufficient to wash out soluble salts supplied with irrigation water. Results showed that as much as 14.4% water could be saved when this strategy was adopted for onion crops.     

The measurement of three cytokine transcripts in naïve and sensitized ovine peripheral blood mononuclear cells following in vitro stimulation with bluetongue virus serotype-23

Bluetongue (BT), a serious disease of sheep and some wild ruminants, is caused by bluetongue virus (BTV), a member of the family, Reoviridae. The current research thrust for controlling BT is on development of efficient vaccines, necessitating clear understanding of ovine immunology. At present, comparative studies on cytokine gene expression profiles of naïve and BTV-sensitized peripheral blood mononuclear cells (PBMC) in sheep have not been clearly understood. In the present study, PBMC from naïve and BEI-inactivated-saponin-adjuvanted BTV-1 vaccinated sheep were stimulated in vitro with heterologous BTV-23. At various intervals, RT-qPCR was carried out to estimate cytokine (interferon-gamma, interleukin-12 and interleukin-2) mRNA expressions that are linked to cell-mediated immunity. The results showed that PBMC cytokine profiles were relatively increased both temporally and quantitatively in immunized sheep PBMC compared to naïve ones, suggesting that BTV-1 vaccination may prime immune system that can cross-react with BTV-23 antigens.     

Metal concentrations,growth, and yield of potato produced from In Vitro plantlets or microtubers and grown in municipal solid‐waste‐amended substrates

In vitro plantlets or microtubers (in vitro produced tubers) of ‘Spunta’ potato (Solanum tuberosum L.) were planted in a 3 soil: 2 peat moss: 1 sand substrate (by volume) amended with municipal solid waste (MS W) compost at 0, 10, 20, or 30 g 4^‐1 L pot. Three months later, plant growth and tuber yield were evaluated and concentrations of shoot and tuber tin (Sn), arsenic (As), copper (Cu), zinc (Zn), nickel (Ni), lead (Pb), manganese (Mn), cadmium (Cd), and iron (Fe) were determined. Amending with MSW resulted in significant increases in concentrations of all tested metals in the substrate. Number of proliferated shoots of plants started from rooted plantlets was greatest at 10 g pot^‐1 MSW, whereas shoot weight of plants started from microtubers was greatest at 10 and 20 g pot^‐1 MSW. Tuber yield of plants started from rooted plantlets or microtubers was greatest at 10 or 30 g pot^‐1 MSW, respectively. In all instances, amending with MSW at 30 g pot^‐1 resulted in significant increases in concentrations of all tested metals in shoots and tubers. Concentrations of shoot Ni and tuber Zn and Fe for plants started from rooted plantlets and concentrations of shoot Fe and tuber As, Cu and Pb for plants started from microtubers increased consistently with increasing MSW percentage of the substrate. Plants started from rooted plantlets produced shoots with sufficient Zn, Mn, and Ni concentrations regardless of the substrate but with toxic Cu content at 30 g pot^‐1 MSW. Plants started from microtubers produced shoots with sufficient Mn and Ni concentrations regardless of the substrate but with low Zn and deficient Cu in unamended substrates. All plants had shoot Fe content higher than the sufficiency range. Although there were significant differences in concentrations of some nutrients among MSW treatments, no symptoms of nutrient toxicity or deficiency were observed. In all instances, tested elements did not accumulate in tubers to levels hazardous to human health. Concentrations of Cd, the most hazardous element, in potato tubers was not high enough to pose a threat to human. Our results indicate that there is a potential use of MSW in satisfying the needs of potato growth with negligible increases in heavy metal concentrations in tubers.     

Evidence for bovine besnoitiosis in Egypt—first serosurvey of Besnoitia besnoiti in cattle and water buffalo (Bubalus bubalis) in Egypt

The present study evaluated the presence of specific antibodies against Besnoitia besnoiti in cattle and water buffalo (Bubalus bubalis) in Egypt. Sera from cattle (n?=?216) and water buffaloes (n?=?133) collected from five different provinces of Egypt (Behera, Alexandria, Assuit, Gharbia, and Matrouh) were analyzed. Testing for B. besnoiti antibodies by PrioCHECK® Besnoitia Ab 2.0 ELISA initially identified 13.75 % (48 out of 349) of individual sera as positive at the manufacturer’s suggested cutoff threshold, 15 percent positivity (PP). Statistically significant associations between B. besnoiti prevalence, species, sex, age, and geographical distribution were observed. Seropositive animals were distributed in all of the provinces from which animals were sampled except Gharbia province. Assuit province showed the highest percentage of infection (30.76 %) followed by Matrouh, Alexandria, and Behera provinces (25, 16.29, and 9.6 %, respectively). The highest infection rate of B. besnoiti was significantly higher in cattle (17.13 %) than in water buffaloes (9.02 %). Positive cases were observed in all age categories. While the highest infection rate (17.13 %) was recorded in the age group 5–10 years followed by the age group 1–5 years (15.38 %), and only one positive case (1.58 %) was recorded in the age group less than 1 year. The highest infection rate of B. besnoiti infection was recorded in the female animals (14.95 %) followed by the male animals (8.33). This is the first report on the detection of B. besnoiti in cattle and water buffaloes in Egypt.     

Rabies – epidemiology,pathogenesis, public health concerns and advances in diagnosis and control: a comprehensive review

Rabies is a zoonotic, fatal and progressive neurological infection caused by rabies virus of the genus Lyssavirus and family Rhabdoviridae. It affects all warm-blooded animals and the disease is prevalent throughout the world and endemic in many countries except in Islands like Australia and Antarctica. Over 60,000 peoples die every year due to rabies, while approximately 15 million people receive rabies post-exposure prophylaxis (PEP) annually. Bite of rabid animals and saliva of infected host are mainly responsible for transmission and wildlife like raccoons, skunks, bats and foxes are main reservoirs for rabies. The incubation period is highly variable from 2 weeks to 6 years (avg. 2–3 months). Though severe neurologic signs and fatal outcome, neuropathological lesions are relatively mild. Rabies virus exploits various mechanisms to evade the host immune responses. Being a major zoonosis, precise and rapid diagnosis is important for early treatment and effective prevention and control measures. Traditional rapid Seller's staining and histopathological methods are still in use for diagnosis of rabies. Direct immunofluoroscent test (dFAT) is gold standard test and most commonly recommended for diagnosis of rabies in fresh brain tissues of dogs by both OIE and WHO. Mouse inoculation test (MIT) and polymerase chain reaction (PCR) are superior and used for routine diagnosis. Vaccination with live attenuated or inactivated viruses, DNA and recombinant vaccines can be done in endemic areas. This review describes in detail about epidemiology, transmission, pathogenesis, advances in diagnosis, vaccination and therapeutic approaches along with appropriate prevention and control strategies.     

Relationships among the composition and physicochemical properties of starches with the characteristics of their films

The physical, molecular, and functional properties of corn, cassava, and yam starches were related to the film properties of these starches. Corn, cassava, and yam starches contained 25%, 19%, and 30% amylose, respectively. Amylose from yam starch showed the smallest molecular weight among the starches and amylopectin from corn starch the smallest molecular weight. Cassava starch presented a higher amylopectin content, and its gels and films were less strong, more transparent, and more flexible than corn and yam films. Plasticized films of the three starches were more flexible, with a higher strain and lower stress at break when the glycerol content increased. Unplasticized films were brittle and had water vapor permeability values ranging from 6.75 x 10(-10) to 8.33 x 10(-10) g m(-1) s(-1) Pa(-1). These values decreased when the glycerol content reached 20 g/100 g of starch because a more compact structure was formed. Then, at a glycerol content of 40 g/100 g of starch, the WVP increased because the film matrixes became less dense.     

A comparison of intradermal and intravenous inoculation of bluetongue virus serotype 23 in sheep for clinico-pathology, and viral and immune responses

The pathogenesis of bluetongue (BT) could vary with route of inoculation. Using laboratory-passaged moderately virulent bluetongue virus serotype 23 (BTV-23), one of the most prevalent Indian serotype, we investigated the pathogenesis of BT in intradermally (ID) and intravenously (IV) inoculated native sheep. The ID inoculation resulted in relatively increased clinical signs and lesions in many organs as compared to IV inoculation. BTV-23 detection by real-time RT-PCR and isolation studies revealed that ID inoculation can be more efficient than IV ones in disseminating and spreading virus to systemic organs, including pre-scapular draining lymph node, spleen, lungs and pulmonary artery. Furthermore, the ID inoculation resulted in early onset and increased humoral response with significant increase (P<0.01) in antibody titre at various intervals. Taken together, these data suggest that ID inoculation can be more potent in reproducing many aspects of natural infection, including clinical disease, viral and immune responses, and may be useful route in setting up experimental infections for challenge or pathogenesis studies using laboratory passaged BTVs.