全文获取类型
收费全文 | 331篇 |
免费 | 22篇 |
国内免费 | 3篇 |
专业分类
林业 | 20篇 |
农学 | 11篇 |
基础科学 | 4篇 |
66篇 | |
综合类 | 17篇 |
农作物 | 13篇 |
水产渔业 | 29篇 |
畜牧兽医 | 164篇 |
园艺 | 11篇 |
植物保护 | 21篇 |
出版年
2023年 | 4篇 |
2022年 | 10篇 |
2021年 | 14篇 |
2020年 | 11篇 |
2019年 | 8篇 |
2018年 | 12篇 |
2017年 | 11篇 |
2016年 | 12篇 |
2015年 | 11篇 |
2014年 | 17篇 |
2013年 | 19篇 |
2012年 | 33篇 |
2011年 | 24篇 |
2010年 | 14篇 |
2009年 | 16篇 |
2008年 | 26篇 |
2007年 | 22篇 |
2006年 | 15篇 |
2005年 | 15篇 |
2004年 | 10篇 |
2003年 | 14篇 |
2002年 | 21篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1990年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
排序方式: 共有356条查询结果,搜索用时 687 毫秒
321.
Nanase Kubo Mari Nishii Mayuko Osada-Oka Hajime Hatta 《The Journal of Poultry Science》2021,58(3):192
Objectives: Atopic dermatitis (AD) is one of the most common skin disorders in infants and children and is often aggravated by increased Staphylococcus aureus (S. aureus) colonization. An inhibitory effect of a specific egg yolk antibody (IgY) on S. aureus growth was demonstrated in this study. Furthermore, the effects of water- or oil-based adjuvants on the preparation of anti-S. aureus IgY and hen immunization were compared.Methods: Hens were immunized intramuscularly with formalin-killed S. aureus mixed with either a water-soluble polysaccharide λ-carrageenan, oil-based Freund''s complete adjuvant (FCA), or Freund''s incomplete adjuvant (FIA). Anti-S. aureus IgYs (FIA-IgY, FCA/FIA-IgY, and λCarra-IgY) were purified from the egg yolk of immunized hen eggs, and the activity of the IgY against S. aureus antigen was measured by ELISA. The proportion of each IgY that was absorbed by S. aureus was also determined. Then, the effect of purified anti-S. aureus IgY on S. aureus growth inhibition was investigated in vitro.Results: The yolk of eggs and purified FIA-IgY from the FIA group showed the highest antibody activity, followed by FCA/FIA-IgY and λCarra-IgY. The proportion of each IgY that was absorbed by S. aureus antigen was as follows: FIA-IgY (18.1%), FCA/FIA-IgY (12.9%), and λCarra-IgY (7.0%). Only FIA-IgY significantly inhibited S. aureus growth in liquid medium.Conclusion: A specific IgY that was produced using the FIA adjutant inhibited S. aureus growth. Although water-soluble λ-carrageenan showed an adjuvant effect on anti-S. aureus IgY induction in egg yolk, but did not inhibit S. aureus growth. The use of the oil adjuvant FIA was necessary in the preparation of anti-S. aureus IgY as a treatment for AD symptoms. 相似文献
322.
The allelic polymorphism and inheritance of MdACS1 and MdACO1 genes were analysed in 28 apple cultivars, which were derived from reciprocal crosses of the following parental pairs: ‘Golden Delicious’ × ‘James Grieve’, ‘Golden Delicious’ × ‘Jonathan’, ‘Cox's Orange Pippin’ × ‘Golden Delicious’, ‘Cox's Orange Pippin’ × ‘Jonathan’. Polymorphisms were detected by PCR and the use of two restriction enzymes (BamH1 and RsaI). In addition, new primers were designed for the further discrimination of MdACO1 alleles. Two alleles of MdACS1 gene (MdACS1‐1 and MdACS1‐2) and three alleles of MdACO1 gene (a, b and c) were detected. Cloning and sequencing of MdACO1 alleles confirmed a high conservation and some differences within the coding regions and helped to reconcile between different numbering systems. Observed segregations confirmed that alleles a, b and c belonged to the MdACO1 gene. It is apparent that polymorphisms within the MdACS1 and MdACO1 genes could aid cultivar genotyping and identification and, furthermore, that the MdACS1‐2/2 genotype is correlated with a long apple storage life. 相似文献
323.
Aleš Mráček Marián Lehocký Petr Smolka Ondřej Grulich Vladimír Velebný 《Fibers and Polymers》2010,11(8):1106-1110
This article describes the novel possibility of the polyester (polyethylene terephthalate) surface modification by plasma
treatment. Moreover, this modified polyester could be component for the composite material (with hyaluronic acid) used in
the wound healing. In this study, the experimental methods: FT-IR spectroscopy, the contact angle measurement, X-ray photoelectron
spectroscopy and scanning electron microscopy were used for the surface modified polyester characterisation. 相似文献
324.
325.
326.
Better soil disinfestation methods, such as biological soil disinfestation (BSD), that are environmentally safe are increasingly
been developed and used because of rising concerns related to environmental risks. We evaluated the efficacy of soil disinfestation
using ethanol to control the fungus Fusarium
oxysporum f. sp. lycopersici, which causes fusarium wilt of tomato. Survival of bud cells and chlamydospores declined markedly in soil saturated with
diluted ethanol solution in the laboratory. In field trials, artificially added nonpathogenic Fusarium
oxysporum and indigenous F. oxysporum were both strongly suppressed in soil saturated with 1% ethanol solution; a wheat bran treatment was not as effective. The
artificially added fungus was not detected in three of four sites treated with ethanol but was detected in three of four sites
amended with wheat bran. Using ethanol in pre-autoclaved soil was not suppressive; thus native microorganisms are essential
for the suppression. This ethanol-mediated biological soil disinfestation (Et-BSD) temporarily increased the number of anaerobic
bacteria, but the number of fungi and aerobic bacteria was stable. Polymerase chain reaction–denaturing gradient gel electrophoresis
(PCR–DGGE) analysis revealed slight but apparent differences in bacterial community structures in the soil treated with Et-BSD
compared with the structure in soils after other treatments such as water irrigation and in the control soil, which received
neither organic amendment nor irrigation after 15 days. Et-BSD is a potentially effective and easy soil disinfestation method,
and its impact on native, beneficial microorganisms is moderate. 相似文献
327.
Treatment of canine parvoviral enteritis with interferon-omega in a placebo-controlled field trial 总被引:2,自引:0,他引:2
The clinical efficacy of a recombinant feline interferon (IFN) (type omega) was evaluated under field conditions for the treatment of dogs with parvoviral enteritis. In this multicentric, double-blind, placebo-controlled trial, 94 dogs from one to 28 months old were randomly assigned to two groups which were treated intravenously either with IFN (2.5 million units/kg) or placebo once a day for three consecutive days, and monitored for clinical signs and mortality for 10 days. Each dog received individual supportive treatment The data from 92 interpretable cases (43 IFN-treated and 49 placebo) showed that the clinical signs of the IFN-treated animals improved significantly in comparison with the control animals, and that there were only three deaths in the IFN group compared with 14 deaths in the placebo group (P = 0.0096) corresponding to a 4.4-fold reduction. Alternative analyses of the data taking into account the prior vaccination status of the dogs against canine parvovirus suggested that the IFN therapy resulted in a 6.4-fold reduction in mortality (P = 0.044) in the unvaccinated cohort, a significant reduction when compared with the vaccinated cohort. 相似文献
328.
We report an unusual case of orbital cellulitis in a cat. Drainage via the pterygopalatine fossa and medical treatment were performed. Failure of resolution and loss of ocular structure and visual function with a poor prognosis led to enucleation. During surgery, plant material and purulent exudate were present in the orbit. Histopathology showed an intraocular foreign body of grass that had migrated through the sclera causing a suprachoroidal abscess with choroidal and retinal detachment. 相似文献
329.
330.