Variation of soil enzyme activity and microbial biomass in poplar plantations of different genotypes and stem spacings

To improve the productivity of poplar plantations, a field experiment of split-plot design with four tree spacings and three poplar clones was established, and four soil enzyme activities and microbial biomass were monitored in the trial.Soil enzyme activities, in most cases,were significantly higher in topsoil(0–10 cm) than in lower horizons(10–20 cm).Soil cellulase, catalase and protease activities during the growing season were higher than during the non-growing season, while invertase activity followed the opposite trend.Soil invertase, cellulase and catalase activities varied by poplar clone but soil protease activity did not.Cellulase and protease activities in the plantation at 5×5 m spacing were significantly higher than in the other spacings.The highest catalase activity was recorded at 6×6 m spacing.At the same planting density, invertase activity was greater in square spacings than in rectangular spacings.Soil microbial biomass was also significantly affected by seedling spacing and poplar clone.The mean soil MBC was significantly lower in topsoil than in the lower horizon, while MBN showed the opposite pattern.Significantly positive correlations were observed among soil cellulase, protease and catalase activities(p0.01), whereas soil invertase activity was negatively and significantly correlated with cellulase, protease and catalase activities(p0.01).Soil microbial biomass and enzyme activities were not correlated except for a significantly negative correlation between soil MBC and catalase activities.Variations in soil enzyme activity and microbial biomass in different poplar plantations suggest that genotype and planting spacing should be considered when modeling soil nutrient dynamics and managing for long-term site productivity.     

Effects of forest harvesting on runoff and sediment characteristics in the Hyrcanian forests,northern Iran

Loss of canopy cover by forest harvesting generally increases the average surface runoff volume and sediment. Selective cutting (single and group selection method) is the most usual forest harvesting method in the Hyrcanian forests in the north of Iran. The purpose of this study is to find the effect of selective logging technique on the hydrological behavior of runoff and sediment in the Kheyrud forests located in northern Iran over 1 year. Four treatments were implemented: natural forest without harvesting (C), forest with selective harvesting (H) and area without canopy cover (WC) and skid trail (S). Three types of data were measured in each plot including soil chemical and physical properties, runoff and sediment load after each rainfall. The results indicate significant differences (P ≤ 0.05) in runoff generation and sediment production with respect to the treatments cover. The runoff in all treatments showed relatively similar response to rainfall, while the highest runoff and sediment were observed in skid trails, and the area without canopy cover (1.13 and 0.62 mm, and 1.2 and 0.51 g m^?2), averagely. In contrast, the natural forest without harvesting and the forest with selective harvesting treatments exhibited the lowest amounts of runoff (0.2 and 0.44 mm) and sediment (0.1 and 0.17 g m^?2), averagely. Implementation of low logging technique was useful to control the effects of logging on the runoff and sediment yield.     

First record of the entomopathogenic fungus <Emphasis Type="Italic">Entomophaga aulicae</Emphasis> on the Bihar hairy caterpillar <Emphasis Type="Italic">Spilarctia obliqua</Emphasis> in Manipur,India

Entomophaga aulicae (Hoffman in Bail) Batko (Zygomycotina: Entomophthorales) was found to cause epizootics in Spilarctia obliqua (Walker) (Arctiidae: Lepidoptera), a polyphagous pest in Manipur, India. Infected caterpillars climbed to elevated positions on plants where they hung upside down, attached by their abdominal prolegs. Infection levels reached up to 57%. The conidiophores are simple, conidia are ovoid to pyriform in shape, and the nuclei stained readily in aceto-orcein. Secondary, tertiary conidia and resting spores were also observed. No rhizoids and cystidia were found. This is the first report of E. aulicae infecting S. obliqua in Manipur.     

A novel biodegradable polyurethane based on hydroxylated polylactic acid and tung oil mixtures. I. Synthesis,physicochemical and biodegradability characterization

A novel biodegradable polylactic acid-based polyurethane (PU) was synthesized via a chain extension reaction between hydroxylated polylactic acid (PLA-OH) and hydroxylated tung oil (HTO) using 1,6-hexamethylene diisocyanate (HDI) to link the two polyols and dibutyltin dilaurate (DBTDL) as a catalyst. Both PLA-OH and HTO, as polyols, were separately synthesized in our laboratory. Three different molecular weights of PLA-OH prepolymers were used, and the molar ratio of PLA-OH to HTO was also changed to investigate the effect of these two parameters on the structure and properties of the final PUs. Chemical structures of PLA-OH, HTO, and final PUs were investigated by Fourier transform infrared (FTIR) and Hydrogen-1 nuclear magnetic resonance (¹HNMR) spectroscopies. Thermal transitions and thermal stability of the final PUs were, respectively, studied by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The FTIR and ¹HNMR results showed that the chain-extension reaction of the two polyols with HDI was sufficiently achieved. The TGA results showed that the polyurethanes based on the lower molecular weight PLA segments were more thermally stable; it was not degraded up to 270 °C. DSC results showed that incorporating HTO in the PU chains led to formation of more flexible PU chains, while the glass transition temperatures of the PUs of higher PLA-OH molecular weights were higher than those of lower ones.     

Spatial heterogeneity of soil organic carbon in tree-based intercropping systems in Quebec and Ontario,Canada

Land use affects the carbon sequestration potential of soils across landscapes. Tree-based intercropping (TBI) systems where annual crops are grown between established tree rows are expected to exhibit spatial heterogeneity in the soil organic carbon (SOC) content due to differences in carbon input and decomposition rates of litter from trees and herbaceous plants. This study aimed to quantify variability in the SOC of TBI systems, compare the SOC content of TBI and nearby conventional agroecosystems, and determine if SOC was related to soil fertility. The TBI research sites were established 4 years (St. Paulin and St. Edouard, Quebec, Canada), 8 years (St. Remi, Quebec, Canada) and 21 years (Guelph, Ontario, Canada) before soil samples were collected for this study. The SOC content was greater within 0.75 m of the tree row than in the intercropped space at the St. Edouard and St. Remi sites. At the Guelph site, the SOC was spatially heterogeneous in plots with Norway spruce (Picea abies L.) but not hybrid poplar (Populus deltoides × P. nigra clone DN-177), probably due to litterfall distribution. Formerly a tree plantation, the TBI system at St. Remi contained 77% more SOC than a nearby conventional agroecosystem, while there was 12% more SOC in the TBI system than the conventional agroecosystem at Guelph. There was no difference in the SOC content of 4-year old TBI sites and nearby conventional agroecosystems. However, an increase in SOC at all TBI sites was positively related to the plant-available N concentrations, indicating the benefit of temperate TBI systems for soil fertility.     

Influence of improved fallow systems and phosphorus application on arbuscular mycorrhizal fungi symbiosis in maize grown in western Kenya

A field study was carried out on a six-year-old on-farm field trial during long-rains season (April–August) 2003 to investigate the effect of improved fallow systems and phosphorus application on arbuscular mycorrhiza fungi (AMF) symbiosis in maize. The trial comprised of maize rotated with a fast growing leguminous Crotalaria grahamiana fallow and a non-leguminous Tithonia diversifolia fallow for 3 years followed by continuous maize. The experiment was randomized complete block design with three cropping (continuous maize, Crotalaria fallow and Tithonia fallow) systems and two phosphorus levels (0 and 50 kg P/ha). AMF colonization in maize roots, maize yield and macro-nutrients uptake were recorded. Phosphorus applications improved (P < 0.05) early (<8 weeks old maize) AMF colonization, nutrient uptake and maize yield in improved fallow systems. Greater differences due to phosphorus application were noted in maize in Tithonia fallow than in Crotalaria fallow. Following phosphorus application, a positive relationship existed between early AMF colonization and maize yield (r = 0.38), and phosphorus and nitrogen uptake (r = 0.40 and r = 0.43, respectively), demonstrating the importance of phosphorus fertilization in enhancing low-input technologies (improved fallows systems) in phosphorus deficient and acidic soils of western Kenya.     

Changes in aboveground and belowground properties during secondary natural succession of a cool-temperate forest in Japan

Forest development in temperate regions is considered to be a global carbon sink. Many studies have examined forest development after harvesting or fire from aboveground (e.g., biomass) or belowground (e.g., soil nutrient) perspectives. However, few studies have explored forest development from both perspectives simultaneously in cool-temperate forests in Japan. In this study, we examined changes over 105 years in both aboveground and belowground components during secondary natural succession. The aboveground biomass increased for 50 years and reached a plateau in a 105-year-old stand. The N mineralization rate increased during succession for 50 years, but showed a decline in the 105-year-old stand due to the decrease in the nitrification rate in late succession. The percent nitrification (i.e., relative contribution of nitrification to N mineralization) decreased significantly with increasing forest stand age. The N mineralization rates had significant relationships with N concentrations of the dominant tree foliage and litter fall and with the amount of litter fall N. Meanwhile, other belowground properties (i.e., soil pH, phenol concentration, soil microbial respiration, and litter mass loss) did not show any significant relationship with forest stand age. This may be because the soil at the study sites was heterogeneous and consisted of Cambisols and Andosols, the latter of which originally has high organic matter content, and thus may have buffered the effect of the aboveground development. These results indicate that belowground N dynamics are more closely associated with aboveground development than other belowground properties in these forests.     

Interlaboratory tests for resistance to Synchytrium endobioticum in potato by the Glynne‐Lemmerzahl method

Synchytrium endobioticum is a major quarantine pathogen of potato causing potato wart disease. In Europe, the pathotypes 1(D1), 2(G1), 6(O1) and 18(T1) are the most widespread and occur locally in almost all countries. Resistance to this disease in potato cultivars is tested for in the majority of the EU countries by the Glynne‐Lemmerzahl method. This paper describes the results of testing two different protocols of this method in five laboratories of three different countries (Germany, Poland, and the Netherlands). The four pathotypes 1(D1), 2(G1), 6(O1) and 18(T1) were tested mainly on the cultivars described in the EPPO Standard PM 7/28 Synchytrium endobioticum. For pathotype 1(D1) and in most cases also for pathotype 18(T1), the results of the cultivars tested were identical to the rating in the EPPO Standard for both protocols. For pathotypes 2(G1) and 6(O1), the cultivars Désirée, Delcora and Miriam showed different results between laboratories as well as between the two protocols. In conclusion, further research is needed to develop one harmonised methodology for resistance testing of potato cultivars to the pathotypes 2(G1), 6(O1) and 18(T1) and to develop a new differential set of potato cultivars for the identification of pathotypes of S. endobioticum.     

Trade-off between height growth and spring flushing in common beech (<Emphasis Type="Italic">Fagus sylvatica</Emphasis> L.)

• Introduction   

The annual development cycle of boreal and temperate trees results from an evolutionary trade-off between two opposing forces. These are namely, the adjustment of leaf phenology to the timing of frost occurrence at the beginning and/or the end of the growth season countered by an effective adjustment to the duration of the growth season to maximise photosynthesis and biomass production during the growing season.     

Spatial Inference of Nitrate Concentrations in Groundwater

We develop a method for multiscale estimation of pollutant concentrations, based on a nonparametric spatial statistical model. We apply this method to estimate nitrate concentrations in groundwater over the mid-Atlantic states, using measurements gathered during a period of 10 years. A map of the fine-scale estimated nitrate concentration is obtained, as well as maps of the estimated county-level average nitrate concentration and similar maps at the level of watersheds and other geographic regions. The fine-scale and coarse-scale estimates arise naturally from a single model, without refitting or ad hoc aggregation. As a result, the uncertainty associated with each estimate is available, without approximations relying on high spatial density of measurements or parametric distributional assumptions.