马动脉炎病毒RT-PCR检测方法的建立

根据已报道的马动脉炎病毒基因组保守基因核苷酸序列，设计并合成了1对引物，通过对影响PCR扩增因素的筛选，成功地从病毒感染的细胞中扩增出约200bp的片段，与理论设计值(204bp)大小一致。而正常的RK-13、BHK-21和Vero细胞和同为动脉炎病毒科的猪繁殖与呼吸道综合征病毒(PRRSV)作为对照的扩增结果均为阴性。敏感性试验表明，该方法可以检测出10^-4个TCID50的病毒含量，说明具有较好的敏感性。     

无公害肉鸡生产的关键技术

肉鸡业是我国畜牧业的重要组成部分，肉鸡产业化经营推动了地方农村经济的发展，同时也大大降低了肉鸡业的投资和生产成本，我国肉鸡业正在向高度集约化、工厂化的方向发展。像其他工业生产一样，这种高效率生产肉产品的企业也存在废物处理、产品的安全性及生产过程中对大气、水体和土壤的污染问题，这些问题如果处理不当就会造成公害。本文就目前肉鸡业存在的公害，     

隐性白肉鸡羽速自别雌雄系的选育(1)

本研究根据现代遗传学基因测交理论,共用32只表型慢羽公鸡与245只快羽母鸡组成测交试验.结果表明,所测的32只公鸡无一只为纯合慢羽基因型.但发现测交后代的快慢羽分布比例与孟德尔遗传上的分离定律完全吻合.同时通过混合精液输精,实施扩群繁育,其中选留慢羽种母雏1460只,表型慢羽种公雏150只,从而为建立隐性白鸡慢羽系奠定了基础.     

绵羊肺腺瘤病毒NM株前病毒gag基因的克隆与序列分析

参照GenBank中已发表的绵羊肺腺瘤病毒(JSRV)的全基因序列，设计合成3对引物，对JSRVNM株的gag基因分3段进行PCR扩增，经琼脂糖凝胶电泳分析，分别呈3条531、888和949 bp的特异条带，将其分别克隆人pMD-18T载体中，进行序列测定并拼接序列，得到完整的gag基因序列。分析结果表明，与南非代表株(基因序列号NC-001494)的gag基因序列比较，核苷酸同源性为89．0%，推导出的氨基酸同源性为90％。与美国代表株(基因序列号AF105220)的gag基因序列比较，核苷酸同源性为86．3％，氨基酸同源性为87％。     

The IMPDH inhibitors,ribavirin and mycophenolic acid,inhibit peste des petits ruminants virus infection

Peste des petits ruminants virus (PPRV) causes highly contagious diseases in domestic and particular wild small ruminants, leading to substantial economic loss. The development of effective and cheap antiviral medications shall help to circumvent this emerging burden. In this study, we found that ribavirin, a competitive inosine-5’-monophosphate dehydrogenase (IMPDH) inhibitor, significantly inhibits the replication of PPRV. As IMPDH is a key enzyme in purine nucleotide synthesis, supplementation of exogenous guanosine attenuate the anti-PPRV effect of ribavirin. Interestingly, an uncompetitive IMPDH inhibitor, mycophenolic acid (MPA), exerted more potent antiviral effect again PPRV. Similarly, this effect was largely restored upon supplementation of guanosine. Thus, we have demonstrated that the IMPDH inhibitors ribavirin and MPA combat PPRV infection through purine nucleotide depletion. Because both regimens have been widely used in the clinic for treating viral infection or organ rejection in transplantation patients for decades, respectively, repurposing these existing safe and cheap medications may provide a new avenue for combating PPRV infection.     

Based serum metabolomics analysis reveals simultaneous interconnecting changes during chicken embryonic development

Metabolic disorder is a major health problem and is associated with a number of metabolic diseases. Due to native hyperglycaemia and resistance to exogenous insulin, chickens as a model had used in the studies of adipose tissue biology, metabolism and obesity. But no detailed information is available about the comprehensive changes of serum metabolites at different stages of chicken embryonic development. This study employed LC/MS‐QTOF to determine the changes of major functional metabolites at incubation day 14 (E14d), 19 (E19d) and hatching day 1 (H1d), and the associated pathways of differential metabolites during chicken embryonic development were analysed using Metabolite Set Enrichment Analysis method. Results showed that 39 metabolites were significantly changed from E14d to E19d and 68 metabolites were significantly altered from E19d to H1d in chicken embryos. Protein synthesis was promoted by increasing the concentrations of L‐glutamine and threonine, and gonadal development was promoted through increasing oestrone content from E14d to E19d in chicken embryos, which indicated that serum glutamine, threonine and oestrone contents may be considered as the candidate indicators for assessment of early embryonic development. 2‐oxoglutaric acid mainly contributed to enhancing the citric cycle, and it plays an important role in improving the growth of chicken embryos at the late development; the decreasing of L‐glutamine, L‐isoleucine and L‐leucine contents from E19d to H1d in chicken embryonic development implied their possible functions as the feed additive during early posthatch period of broiler chickens to satisfy the growth. These results provided insights into understand the roles of serum metabolites at different developmental stages of chicken embryos, it also provides available information for chicken as a model to study metabolic disease or human obesity.     

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation

Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane‐impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca²⁺ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm. A low concentration of sucrose plus trehalose (S₁₅T₁₅) was optimal. Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L₁₅T₁₅) gave the best results for both A. sinensis and A. dabryanus. Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting. Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis. The results showed that relative ENO3 and PMCA2 expression levels were the highest at L₁₅T₁₅ in cryopreserved A. sinensis sperm. There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight‐line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm. Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.     

基于冗余分析的高寒草原土壤与草地退化关系

在高寒草原退化草地设置研究样地,观测植物群落特征、采集土壤样品、分析土壤物理化学性质,依据数量生态学基本原理,探讨高寒草原退化草地与土壤因子间关系。结果表明:不同退化程度样地沿冗余分析排序图第一排序轴分布,第一排序轴反映草地退化程度的变化;草地植物群落中与第一排序轴负相关且按相关程度大小排序的指标为盖度>地上生物量>地下生物量;第一、第二排序轴能够解释97.1%的土壤因子与草地退化关系;土壤沙砾、p H与第一排序轴正相关,土壤有机碳、土壤含水量、容重、全氮、有效氮与第一排序轴负相关;第一排序轴及所有排序轴所反映的土壤因子均与草地退化之间呈极显著相关关系(P<0.01);不同土壤因子与草地退化之间相关程度不同,土壤有机碳(r=-0.890)、土壤含水量(r=-0.864)、容重(r=-0.847)、全氮(r=-0.836)、有效氮(r=-0.821)等与高寒草原退化相关程度更高且相关关系极显著(P<0.01)。利用退化草地群落特征和土壤因子数据矩阵进行冗余分析能够较好地综合反映土壤因子与草地退化之间的关系及相关程度,土壤有机碳、土壤含水量、容重、全氮、有效氮是反映高寒草原退化的重要土壤指标。